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1.
Article in English | MEDLINE | ID: mdl-36717268

ABSTRACT

BACKGROUND AND OBJECTIVE: Depleting CD20+ B cells is the primary mechanism by which ocrelizumab (OCRE) is efficient in persons with multiple sclerosis (pwMS). However, the exact role of OCRE on other immune cell subsets directly or indirectly remains elusive. The purpose of this study is to characterize the dynamics of peripheral immune cells of pwMS on OCRE. METHODS: We collected blood samples from 38 pwMS before OCRE onset (T0) and at 6 and 12 months (T6, T12) after initiation. To cover the immune cell diversity, using mass cytometry time of flight, we designed a 38-parameter panel to analyze B, T, and innate immune cell markers and CNS migratory markers. In parallel, viral-specific CD8+ T-cell responses were assessed by the quantification of interferon-γ secretion using the enzyme-linked immunospot assay on cytomegalovirus, Epstein-Barr virus, and influenza stimulations. RESULTS: Beside B-cell depletion, we observed a loss in memory CD8+CD20+ and central memory CD8+ T cells but not in CD4+CD20+ T cells already at T6 and T12 (p < 0.001). The loss of memory CD8+ T cells correlated with a lower CXCR3 expression (p < 0.001) and CNS-related LFA-1 integrin expression (p < 0.001) as well as a reduced antiviral cellular immune response observed at both time points (p < 0.001). Of note, we did not observe major changes in the phenotype of the other cell types studied. Seven of 38 (18.4%) patients in our cohort presented with infections while on OCRE; 4 of which were switched from dimethyl fumarate. Finally, using a mixed linear model on mass cytometry data, we demonstrated that the immunomodulation induced by previous disease-modifying therapies (DMTs) was prolonged over the period of the study. DISCUSSION: In addition to its well-known role on B cells, our data suggest that OCRE also acts on CD8+ T cells by depleting the memory compartment. These changes in CD8+ T cells may be an asset in the action of OCRE on MS course but might also contribute to explain the increased occurrence of infections in these patients. Finally, although more data are needed to confirm this observation, it suggests that clinicians should pay a special attention to an increased infection risk in pwMS switched from other DMTs to OCRE.


Subject(s)
Epstein-Barr Virus Infections , Multiple Sclerosis , Humans , CD8-Positive T-Lymphocytes , Herpesvirus 4, Human , Epstein-Barr Virus Infections/metabolism , Longitudinal Studies , Phenotype
2.
Plant Physiol ; 180(1): 185-197, 2019 05.
Article in English | MEDLINE | ID: mdl-30837347

ABSTRACT

Thiamin pyrophosphate (TPP) is the active form of vitamin B1 and works as an essential cofactor for enzymes in key metabolic pathways, such as the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. Although its action as a coenzyme has been well documented, the roles of TPP in plant metabolism are still not fully understood. Here, we investigated the functions of TPP in the regulation of the metabolic networks during photoperiod transition using previously described Arabidopsis (Arabidopsis thaliana) riboswitch mutant plants, which accumulate thiamin vitamers. The results show that photosynthetic and metabolic phenotypes of TPP riboswitch mutants are photoperiod dependent. Additionally, the mutants are more distinct from control plants when plants are transferred from a short-day to a long-day photoperiod, suggesting that TPP also plays a role in metabolic acclimation to the photoperiod. Control plants showed changes in the amplitude of diurnal oscillation in the levels of metabolites, including glycine, maltose, and fumarate, following the photoperiod transition. Interestingly, many of these changes are not present in TPP riboswitch mutant plants, demonstrating their lack of metabolic flexibility. Our results also indicate a close relationship between photorespiration and the TCA cycle, as TPP riboswitch mutants accumulate less photorespiratory intermediates. This study shows the potential role of vitamin B1 in the diurnal regulation of central carbon metabolism in plants and the importance of maintaining appropriate cellular levels of thiamin vitamers for the plant's metabolic flexibility and ability to acclimate to an altered photoperiod.


Subject(s)
Arabidopsis/physiology , Photoperiod , Thiamine Pyrophosphate/metabolism , Acclimatization , Amino Acids/metabolism , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Circadian Rhythm/physiology , Citric Acid Cycle , Gene Expression Regulation, Plant , Iron-Sulfur Proteins/genetics , Iron-Sulfur Proteins/metabolism , Mutation , Riboswitch/genetics
4.
J Biol Chem ; 289(21): 14692-706, 2014 May 23.
Article in English | MEDLINE | ID: mdl-24706747

ABSTRACT

Unwanted enzyme side reactions and spontaneous decomposition of metabolites can lead to a build-up of compounds that compete with natural enzyme substrates and must be dealt with for efficient metabolism. It has recently been realized that there are enzymes that process such compounds, formulating the concept of metabolite repair. NADH and NADPH are vital cellular redox cofactors but can form non-functional hydrates (named NAD(P)HX) spontaneously or enzymatically that compete with enzymes dependent on NAD(P)H, impairing normal enzyme function. Here we report on the functional characterization of components of a potential NAD(P)H repair pathway in plants comprising a stereospecific dehydratase (NNRD) and an epimerase (NNRE), the latter being fused to a vitamin B6 salvage enzyme. Through the use of the recombinant proteins, we show that the ATP-dependent NNRD and NNRE act concomitantly to restore NAD(P)HX to NAD(P)H. NNRD behaves as a tetramer and NNRE as a dimer, but the proteins do not physically interact. In vivo fluorescence analysis demonstrates that the proteins are localized to mitochondria and/or plastids, implicating these as the key organelles where this repair is required. Expression analysis indicates that whereas NNRE is present ubiquitously, NNRD is restricted to seeds but appears to be dispensable during the normal Arabidopsis life cycle.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , NADP/metabolism , NAD/metabolism , Amino Acid Sequence , Arabidopsis/genetics , Arabidopsis Proteins/chemistry , Arabidopsis Proteins/genetics , Blotting, Western , Gene Expression Regulation, Plant , Hydro-Lyases/chemistry , Hydro-Lyases/genetics , Hydro-Lyases/metabolism , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Metabolic Networks and Pathways/genetics , Microscopy, Confocal , Mitochondria/metabolism , Models, Molecular , Molecular Sequence Data , Molecular Structure , Mutation , NAD/chemistry , NADP/chemistry , Plants, Genetically Modified , Plastids/metabolism , Protein Structure, Tertiary , Racemases and Epimerases/chemistry , Racemases and Epimerases/genetics , Racemases and Epimerases/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid
5.
Proc Natl Acad Sci U S A ; 110(36): 14622-7, 2013 Sep 03.
Article in English | MEDLINE | ID: mdl-23959877

ABSTRACT

Thiamin (vitamin B1) is an essential micronutrient needed as a cofactor for many central metabolic enzymes. Animals must have thiamin in their diet, whereas bacteria, fungi, and plants can biosynthesize it de novo from the condensation of a thiazole and a pyrimidine moiety. Although the routes to biosynthesize these two heterocycles are not conserved in different organisms, in all cases exogenous thiamin represses expression of one or more of the biosynthetic pathway genes. One important mechanism for this control is via thiamin-pyrophosphate (TPP) riboswitches, regions of the mRNA to which TPP can bind directly, thus facilitating fine-tuning to maintain homeostasis. However, there is little information on how modulation of riboswitches affects thiamin metabolism in vivo. Here we use the green alga, Chlamydomonas reinhardtii, which regulates both thiazole and pyrimidine biosynthesis with riboswitches in the THI4 (Thiamin 4) and THIC (Thiamin C) genes, respectively, to investigate this question. Our study reveals that regulation of thiamin metabolism is not the simple dogma of negative feedback control. Specifically, balancing the provision of both of the heterocycles of TPP appears to be an important requirement. Furthermore, we show that the Chlamydomonas THIC riboswitch is controlled by hydroxymethylpyrimidine pyrophosphate, as well as TPP, but with an identical alternative splicing mechanism. Similarly, the THI4 gene is responsive to thiazole. The study not only provides insight into the plasticity of the TPP riboswitches but also shows that their maintenance is likely to be a consequence of evolutionary need as a function of the organisms' environment and the particular pathway used.


Subject(s)
Chlamydomonas reinhardtii/genetics , Chlamydomonas reinhardtii/metabolism , Riboswitch/genetics , Thiamine/metabolism , Algal Proteins/genetics , Algal Proteins/metabolism , Alternative Splicing , Biosynthetic Pathways/genetics , Gene Expression Regulation, Plant , Molecular Structure , Phosphotransferases (Phosphate Group Acceptor)/genetics , Phosphotransferases (Phosphate Group Acceptor)/metabolism , Plant Proteins/genetics , Plant Proteins/metabolism , Point Mutation , Protein Binding , Pyrimidines/biosynthesis , Pyrimidines/chemistry , Reverse Transcriptase Polymerase Chain Reaction , Thiamine/chemistry , Thiamine Pyrophosphate/chemistry , Thiamine Pyrophosphate/metabolism , Thiazoles/chemistry , Thiazoles/metabolism
6.
Front Plant Sci ; 4: 160, 2013.
Article in English | MEDLINE | ID: mdl-23755056

ABSTRACT

Thiamin (vitamin B1) is made by plants and microorganisms but is an essential micronutrient in the human diet. All organisms require it as a cofactor in its form as thiamin pyrophosphate (TPP) for the activity of key enzymes of central metabolism. In humans, deficiency is widespread particularly in populations where polished rice is a major component of the diet. Considerable progress has been made on the elucidation of the biosynthesis pathway within the last few years enabling concrete strategies for biofortification purposes to be devised, with a particular focus here on genetic engineering. Furthermore, the vitamin has been shown to play a role in both abiotic and biotic stress responses. The precursors for de novo biosynthesis of thiamin differ between microorganisms and plants. Bacteria use intermediates derived from purine and isoprenoid biosynthesis, whereas the pathway in yeast involves the use of compounds from the vitamin B3 and B6 groups. Plants on the other hand use a combination of the bacterial and yeast pathways and there is subcellular partitioning of the biosynthesis steps. Specifically, thiamin biosynthesis occurs in the chloroplast of plants through the separate formation of the pyrimidine and thiazole moieties, which are then coupled to form thiamin monophosphate (TMP). Phosphorylation of thiamin to form TPP occurs in the cytosol. Therefore, thiamin (or TMP) must be exported from the chloroplast to the cytosol for the latter step to be executed. The regulation of biosynthesis is mediated through riboswitches, where binding of the product TPP to the pre-mRNA of a biosynthetic gene modulates expression. Here we examine and hypothesize on genetic engineering approaches attempting to increase the thiamin content employing knowledge gained with the model plant Arabidopsis thaliana. We will discuss the regulatory steps that need to be taken into consideration and can be used a prerequisite for devising such strategies in crop plants.

7.
Plant J ; 75(1): 40-52, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23551747

ABSTRACT

Vitamin B6 is a cofactor for more than 140 essential enzymatic reactions and was recently proposed as a potent antioxidant, playing a role in the photoprotection of plants. De novo biosynthesis of the vitamin has been described relatively recently and is derived from simple sugar precursors as well as glutamine. In addition, the vitamin can be taken up from exogenous sources in a broad range of organisms, including plants. However, specific transporters have been identified only in yeast. Here we assess the ability of the family of Arabidopsis purine permeases (PUPs) to transport vitamin B6. Several members of the family complement the growth phenotype of a Saccharomyces cerevisiae mutant strain impaired in both de novo biosynthesis of vitamin B6 as well as its uptake. The strongest activity was observed with PUP1 and was confirmed by direct measurement of uptake in yeast as well as in planta, defining PUP1 as a high affinity transporter for pyridoxine. At the tissue level the protein is localised to hydathodes and here we use confocal microscopy to illustrate that at the cellular level it is targeted to the plasma membrane. Interestingly, we observe alterations in pyridoxine recycling from the guttation sap upon overexpression of PUP1 and in a pup1 mutant, consistent with the role of the protein in retrieval of pyridoxine. Furthermore, combining the pup1 mutant with a vitamin B6 de novo biosynthesis mutant (pdx1.3) corroborates that PUP1 is involved in the uptake of the vitamin.


Subject(s)
Arabidopsis/metabolism , Gene Expression Regulation, Plant , Nucleobase Transport Proteins/metabolism , Pyridoxine/metabolism , Arabidopsis/genetics , Arabidopsis/ultrastructure , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Biological Transport , Cell Membrane/metabolism , Gene Expression , Genetic Complementation Test , Microscopy, Confocal , Multigene Family , Mutation , Nucleobase Transport Proteins/genetics , Phenotype , Plant Epidermis/genetics , Plant Epidermis/metabolism , Plant Epidermis/ultrastructure , Plant Exudates/analysis , Plant Roots/genetics , Plant Roots/metabolism , Plant Roots/ultrastructure , Plants, Genetically Modified , Pyridoxine/chemistry , Recombinant Fusion Proteins , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism
8.
Plant J ; 69(4): 589-600, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21988537

ABSTRACT

The Arabidopsis ACCELERATED CELL DEATH 2 (ACD2) protein protects cells from programmed cell death (PCD) caused by endogenous porphyrin-related molecules like red chlorophyll catabolite or exogenous protoporphyrin IX. We previously found that during bacterial infection, ACD2, a chlorophyll breakdown enzyme, localizes to both chloroplasts and mitochondria in leaves. Additionally, acd2 cells show mitochondrial dysfunction. In plants with acd2 and ACD2 (+) sectors, ACD2 functions cell autonomously, implicating a pro-death ACD2 substrate as being cell non-autonomous in promoting the spread of PCD. ACD2 targeted solely to mitochondria can reduce the accumulation of an ACD2 substrate that originates in chloroplasts, indicating that ACD2 substrate molecules are likely to be mobile within cells. Two different light-dependent reactive oxygen bursts in mitochondria play prominent and causal roles in the acd2 PCD phenotype. Finally, ACD2 can complement acd2 when targeted to mitochondria or chloroplasts, respectively, as long as it is catalytically active: the ability to bind substrate is not sufficient for ACD2 to function in vitro or in vivo. Together, the data suggest that ACD2 localizes dynamically during infection to protect cells from pro-death mobile substrate molecules, some of which may originate in chloroplasts, but have major effects on mitochondria.


Subject(s)
Apoptosis Regulatory Proteins/metabolism , Apoptosis/physiology , Arabidopsis Proteins/metabolism , Arabidopsis/enzymology , Mitochondria/enzymology , Oxidoreductases/metabolism , Reactive Oxygen Species/metabolism , Apoptosis Regulatory Proteins/genetics , Arabidopsis/genetics , Arabidopsis/physiology , Arabidopsis/radiation effects , Arabidopsis Proteins/genetics , Chlorophyll/metabolism , Chloroplasts/enzymology , Light , Models, Biological , Mutation , Oxidoreductases/genetics , Phenotype , Plant Leaves/enzymology , Plant Leaves/genetics , Plant Leaves/physiology , Plant Leaves/radiation effects , Respiratory Burst
9.
Biochem Soc Trans ; 39(2): 652-7, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21428956

ABSTRACT

Riboswitches are regions of mRNA to which a metabolite binds in the absence of proteins, resoulting in alteration of transcription, translation or splicing. The most widespread forms of riboswitches are those responsive to TPP (thiamine pyrophosphate) the active form of vitamin B1, thiamine. TPP-riboswitches have been found in all bacterial genomes examined, and are the only ones found in eukaryotes. In each case, the riboswitch appears to regulate the expression of a gene involved in synthesis or uptake of the vitamin. Riboswitches offer an attractive target for chemical intervention, and identification of novel ligands would allow a detailed study on structure-activity relationships, as well as potential leads for the development of antimicrobial compounds. To this end, we have developed a medium-throughput methodology for screening libraries of small molecules using biophysical methods.


Subject(s)
High-Throughput Screening Assays/methods , Ligands , Riboswitch , Thiamine Pyrophosphate/metabolism , Animals , Base Sequence , Humans , Models, Biological , Models, Molecular , Nucleic Acid Conformation , Riboswitch/physiology , Small Molecule Libraries/analysis , Small Molecule Libraries/chemistry , Structure-Activity Relationship , Thiamine Pyrophosphate/chemistry
10.
Sci Rep ; 1: 187, 2011.
Article in English | MEDLINE | ID: mdl-22355702

ABSTRACT

The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. This suggested that it might affect interactions between infected plants and aphids, insects that transmit CMV. We found that infection of tobacco with a 2b gene deletion mutant (CMVΔ2b) induced strong resistance to aphids (Myzus persicae) while CMV infection fostered aphid survival. Using electrical penetration graph methodology we found that higher proportions of aphids showed sustained phloem ingestion on CMV-infected plants than on CMVΔ2b-infected or mock-inoculated plants although this did not increase the rate of growth of individual aphids. This indicates that while CMV infection or certain viral gene products might elicit aphid resistance, the 2b protein normally counteracts this during a wild-type CMV infection. Our findings suggest that the 2b protein could indirectly affect aphid-mediated virus transmission.


Subject(s)
Aphids/physiology , Cucumovirus/genetics , Gene Silencing , Nicotiana/genetics , Viral Proteins/genetics , Animals , Behavior, Animal , Cucumovirus/physiology , Cyclopentanes/metabolism , Feeding Behavior , Gene Deletion , Mutation , Nicotine/metabolism , Oxylipins/metabolism , Phloem/metabolism , Plant Diseases , RNA, Small Interfering/metabolism , Nicotiana/virology , Viral Proteins/physiology
11.
Trends Plant Sci ; 15(9): 488-98, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20598625

ABSTRACT

Tetrapyrroles such as chlorophyll and heme are co-factors for essential proteins involved in a wide variety of crucial cellular functions. Nearly 2% of the proteins encoded by the Arabidopsis thaliana genome are thought to bind tetrapyrroles, demonstrating their central role in plant metabolism. Although the enzymes required for tetrapyrrole biosynthesis are well characterized, there are still major questions about the regulation of the pathway, and the transport of tetrapyrroles within cells. These issues are important, as misregulation of tetrapyrrole metabolism can lead to severe photo-oxidative stress, and because tetrapyrroles have been implicated in signaling pathways coordinating interactions between plant organelles. In this review, we discuss the cell biology of tetrapyrrole metabolism and its implications for tetrapyrroles as signaling molecules.


Subject(s)
Plants/metabolism , Tetrapyrroles/metabolism , Animals , Biological Transport , Carrier Proteins/metabolism , Humans , Plastids/metabolism , Protein Binding
12.
Mol Plant Microbe Interact ; 23(7): 835-45, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20521947

ABSTRACT

The Cucumber mosaic virus (CMV) 2b counter-defense protein disrupts plant antiviral mechanisms mediated by RNA silencing and salicylic acid (SA). We used microarrays to investigate defensive gene expression in 2b-transgenic Arabidopsis thaliana plants. Surprisingly, 2b inhibited expression of few SA-regulated genes and, in some instances, enhanced the effect of SA on certain genes. Strikingly, the 2b protein inhibited changes in the expression of 90% of genes regulated by jasmonic acid (JA). Consistent with this, infection of plants with CMV, but not the 2b gene-deletion mutant CMVDelta2b, strongly inhibited JA-inducible gene expression. JA levels were unaffected by infection with either CMV or CMVDelta2b. Although the CMV-Arabidopsis interaction is a compatible one, SA accumulation, usually considered to be an indicator of plant resistance, was increased in CMV-infected plants but not in CMVDelta2b-infected plants. Thus, the 2b protein inhibits JA signaling at a step downstream of JA biosynthesis but it primes induction of SA biosynthesis by another CMV gene product or by the process of infection itself. Like many plant viruses, CMV is aphid transmitted. JA is important in plant defense against insects. This raises the possibility that disruption of JA-mediated gene expression by the 2b protein may influence CMV transmission by aphids.


Subject(s)
Cucumovirus/metabolism , Gene Expression Regulation, Viral/physiology , RNA Interference/physiology , RNA, Viral/metabolism , Arabidopsis/genetics , Arabidopsis/metabolism , Cucumovirus/genetics , Cyclopentanes/metabolism , Oxylipins/metabolism , Plants, Genetically Modified , RNA, Viral/genetics , Salicylic Acid/metabolism , Signal Transduction/physiology , Viral Proteins/genetics , Viral Proteins/metabolism
13.
Proc Natl Acad Sci U S A ; 105(39): 15178-83, 2008 Sep 30.
Article in English | MEDLINE | ID: mdl-18818314

ABSTRACT

Chloroplast biogenesis involves careful coordination of both plastid and nuclear gene expression, which is achieved in part by retrograde signaling from the chloroplast to the nucleus. This can be demonstrated by the fact that the herbicide, Norflurazon (NF), which causes bleaching of chloroplasts, prevents the light induction of photosynthesis-related genes in the nucleus. It has been proposed that the tetrapyrrole pathway intermediate Mg-protoporphyrin IX acts as the signaling molecule in this pathway and accumulates in the chloroplasts and cytosol of the cell after NF treatment. Here we present data that demonstrate that this model is too simplistic. We have developed a sensitive liquid chromatography-mass spectrometry (LC/MS) method to measure tetrapyrrole intermediates and have shown that no Mg-protoporphyrin IX, nor indeed any other chlorophyll-biosynthesis intermediate, can be detected in NF-treated plants under conditions in which nuclear gene expression is repressed. Conversely when endogenous Mg-protoporphyrin IX levels are artificially increased by supplementation with the tetrapyrrole precursor, 5-aminolevulinic acid, the expression of nuclear-encoded photosynthetic genes is induced, not repressed. We also demonstrate that NF-treatment leads to a strong down-regulation of tetrapyrrole biosynthesis genes, consistent with the absence of an accumulation of tetrapyrrole intermediates. Finally, there is no correlation between nuclear-gene expression and any of the chlorophyll biosynthetic intermediates over a range of growth conditions and treatments. Instead, it is possible that a perturbation of tetrapyrrole synthesis may lead to localized ROS production or an altered redox state of the plastid, which could mediate retrograde signaling.


Subject(s)
Arabidopsis/metabolism , Chlorophyll/biosynthesis , Plastids/metabolism , Protoporphyrins/metabolism , Arabidopsis/drug effects , Arabidopsis/genetics , Arabidopsis/ultrastructure , Cell Nucleus/drug effects , Cell Nucleus/metabolism , Chlorophyll/analysis , Chlorophyll/genetics , Chlorophyll A , Chromatography, Liquid/methods , Gene Expression Regulation, Plant/drug effects , Herbicides/pharmacology , Mass Spectrometry/methods , Plastids/drug effects , Plastids/genetics , Pyridazines/pharmacology , Seedlings/drug effects , Seedlings/genetics , Seedlings/metabolism , Seedlings/ultrastructure , Signal Transduction
14.
Proc Natl Acad Sci U S A ; 104(52): 20770-5, 2007 Dec 26.
Article in English | MEDLINE | ID: mdl-18093957

ABSTRACT

In bacteria, many genes involved in the biosynthesis of cofactors such as thiamine pyrophosphate (TPP) are regulated by ribo switches, regions in the 5' end of mRNAs to which the cofactor binds, thereby affecting translation and/or transcription. TPP riboswitches have now been identified in fungi, in which they alter mRNA splicing. Here, we show that addition of thiamine to cultures of the model green alga Chlamydomonas reinhardtii alters splicing of transcripts for the THI4 and THIC genes, encoding the first enzymes of the thiazole and pyrimidine branches of thiamine biosynthesis, respectively, concomitant with an increase in intracellular thiamine and TPP levels. Comparison with Volvox carteri, a related alga, revealed highly conserved regions within introns of these genes. Inspection of the sequences identified TPP riboswitch motifs, and RNA transcribed from the regions binds TPP in vitro. The THI4 riboswitch, but not the promoter region, was found to be necessary and sufficient for thiamine to repress expression of a luciferase-encoding reporter construct in vivo. The pyr1 mutant of C. reinhardtii, which is resistant to the thiamine analogue pyrithiamine, has a mutation in the THI4 riboswitch that prevents the THI4 gene from being repressed by TPP. By the use of these ribo switches, thiamine biosynthesis in C. reinhardtii can be effectively regulated at physiological concentrations of the vitamin.


Subject(s)
Alternative Splicing , Chlamydomonas reinhardtii/metabolism , Eukaryota/physiology , Gene Expression Regulation , Animals , Base Sequence , Biochemistry/methods , Codon , Eukaryota/metabolism , Genes, Reporter , Luciferases/metabolism , Models, Chemical , Molecular Sequence Data , Mutation , Nucleic Acid Conformation , Photosynthesis , Plasmids/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Thiamine/chemistry , Thiamine/metabolism
15.
Curr Opin Plant Biol ; 10(3): 266-75, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17434786

ABSTRACT

Over recent years, the pathways for the biosynthesis of many vitamins have been elucidated at the molecular level in plants, and several unique features are emerging. One is that the mitochondrion plays an important role in the synthesis of folate (vitamin B9), biotin (B7), pantothenate (B5), ascorbate (C), and possibly thiamin (B1). Second, the production of some of these cofactors is regulated by developmental cues, and perhaps more surprisingly, by environmental signals such as high light and salinity. Moreover, the biosynthesis of thiamin in Arabidopsis may be negatively regulated by a riboswitch, a novel method of gene regulation that is characteristic of cofactor biosynthesis in bacteria. Vitamin B12 is unique in that it is not found in vascular plants, but is abundant in algae; recent molecular work has revealed that algae do not synthesise the vitamin but instead obtain it from bacteria.


Subject(s)
Plants/metabolism , Vitamins/biosynthesis , Biosynthetic Pathways/physiology , Mitochondria/metabolism
16.
Anal Biochem ; 308(2): 320-7, 2002 Sep 15.
Article in English | MEDLINE | ID: mdl-12419346

ABSTRACT

A novel procedure to specifically quantify low amounts of pipecolic acid and structurally related compounds in several types of biological materials has been characterized. From crude extracts of various types of biological material, the first step was to clear all low-molecular-weight compounds containing primary amino groups by a treatment of nitrous acid. Using a microwave-assisted reaction, the remaining substances containing secondary amino groups were then derivatized with ninhydrin and made soluble in glacial acetic acid. The derivatives produced were resolved by reverse-phase HPLC and detected by spectrophotometry at 570nm. This procedure allowed more rapid determination of pipecolic acid since microwave heating shortened the time needed for derivatization compared with heating at 95 degrees C in a water bath. The complete analysis of the chromogens for pipecolic acid and related substances was achieved in 20min. Under such conditions, the detection threshold for pipecolic acid was about 20pmol. The suitability of the technique was assessed in various biological matrices known to contain significant amounts of this amino acid. The data obtained are in accordance with those available in the literature. To our knowledge, this is the first method using the ninhydrin reaction in a precolumn, microwave-assisted derivatization procedure for detection and determination of heterocyclic alpha-amino acids.


Subject(s)
Chromatography, High Pressure Liquid , Indicators and Reagents/chemistry , Ninhydrin/chemistry , Pipecolic Acids/analysis , Brassica napus/chemistry , Brassica napus/drug effects , Brevibacterium/chemistry , Brevibacterium/drug effects , Chromatography, Ion Exchange , Humans , Microwaves , Nitrous Acid/pharmacology , Plant Leaves/chemistry , Plant Leaves/drug effects , Seeds/chemistry , Seeds/drug effects
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