Complete genome sequence and description of Lactococcus garvieae M14 isolated from Algerian fermented milk.

We describe using a polyphasic approach that combines proteomic by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) analysis, genomic data and phenotypic characterization the features of Lactococcus garvieae strain M14 newly isolated from the fermented milk (known as raib) of an Algerian cow. The 2 188 835 bp containing genome sequence displays a metabolic capacity to form acid fermentation that is very useful for industrial applications and encodes for two bacteriocins responsible for its eventual bioprotective properties.

Chloride current modulation during meiosis in Xenopus oocytes.

Using the voltage clamp technique, the calcium-dependent transient outward chloride current in Xenopus oocytes was monitored during in vitro meiotic maturation induced by progesterone. This Cl conductance increased during the first 5-8 hours following progesterone stimulation. It then decreased until germinal vesicle breakdown (GVBD). Before GVBD, the Cl conductance could be activated by A23187 ionophore. After GVBD, the transient current totally disappeared, and only a single voltage-gated current could be observed.