ABSTRACT
OBJECTIVES: To examine the prevalence of Human papillomavirus and Chlamydia trachomatis DNA in cervical samples among women with normal and abnormal cervical cytology from La Plata, Argentina. METHODS: Two hundred and seventy-nine women (200 with cervical neoplasia or ICC and 79 women with normal cytology) provided cervical samples for the detection of HPV and C. trachomatis DNA by PCR-based assays. RESULTS: HPV DNA increased with the cervical lesion severity, ranging from 30% among women with normal cytology to 99-100% among women with HSIL or ICC. C. trachomatis DNA prevalence increased from low levels in women with normal cytology (11%) to 47% in those with HSIL, but was uncommon among ICC patients (20%). Among women with normal cytology, C. trachomatis prevalence was higher in HPV DNA positive (12.5%) than HPV DNA negative women (10.9%), but this difference was not significant. CONCLUSIONS: HPV prevalence in the general population is slightly higher than those reported for other developing countries. C. trachomatis DNA positivity was associated with a higher risk of both LSIL and HSIL lesions, but not with ICC.
Subject(s)
Chlamydia Infections/epidemiology , Chlamydia trachomatis/genetics , Papillomaviridae/genetics , Papillomavirus Infections/epidemiology , Uterine Cervical Diseases/epidemiology , Uterine Cervical Diseases/microbiology , Adolescent , Adult , Aged , Argentina/epidemiology , Chlamydia Infections/microbiology , DNA, Viral/analysis , Female , Humans , Middle Aged , Papillomavirus Infections/virology , Polymerase Chain Reaction , Uterine Cervical Diseases/virology , Uterine Cervical Neoplasms/epidemiology , Uterine Cervical Neoplasms/microbiology , Uterine Cervical Neoplasms/virology , Uterine Cervical Dysplasia/epidemiology , Uterine Cervical Dysplasia/microbiology , Uterine Cervical Dysplasia/virologyABSTRACT
Se analizaron 69 muestras positivas para el virus papiloma humano (VPH) tipos -6, -16 y -18, y 24 muestras con citología cervical normal negativas para el VPH como grupo control. El análisis de la amplificación génica del proto-oncogén HER-2/neu se realizó utilizando la técnica de coamplificación con locus de referencia. Se encontró una asociación entre la amplificación del gen HER-2/neu y el grupo viral de "bajo riesgo" (VPH-6) (p < 0.005). Dentro de este grupo, se observó una asociación entre la amplificación del proto-oncogén y el status citopatológico CIN I (p < 0.01). Debido a que la mayoría de las muestras CIN I analizadas presentaron un patrón coilocítico, la amplificación de HER-2/neu parecería estar relacionada con este tipo de alteración celular. Por otra parte, sería importante estudiar la amplificación génica y la expresión de HER-2/neu en los diferentes estadios de las neoplasias intraepiteliales cervicales a fin de poder evaluar su papel en la progresión del cáncer cervical.