Diameter growth and age of jaboticaba trees (Plinia peruviana (Poir.) Govaerts) native from Southwest of Paraná, Brazil, based on growth-rings.

The jaboticaba tree (Plinia peruviana (Poir.) Govaerts) is a native Brazilian species, and its fruits are very popular in Brazil. The purpose of this study was to model the diameter growth, and determine the age of jaboticaba trees in four natural populations in Southwest of Paraná State, Brazil. The knowledge of the growth dynamics of this species is essential to assist management and conservation strategies and to discover when this species was established in the region. Core samples were collected to obtain complete growth series by measurement of annual growth rings. The series were crossdated, seven growth models were adjusted, and the best model for each site and a general model were selected using statistics rules. The time series spanned periods of ~75 to ~100 years, and growth ranged between 0.27 and 0.37 cm year-1. Chapman-Richards' model showed better adherence for locations individually, and Monomolecular when grouped. Because to the age reached by the jaboticaba trees and the high density of this species in these natural populations, consequently named "jaboticabais", it was inferred that there was an anthropogenic action in the dispersion and formation of the settlements through the indigenous and caboclos that inhabited the region before 1940. Also, this is the first work to register and verify the fusion of trunks for species of the Plinia genus using double piths found in samples, a feature that must be considered to avoid overestimating the age of jaboticaba trees based solely on measuring tree diameter.

Morphophysiological changes of Acca sellowiana (Myrtaceae: Myrtoideae) saplings under shade gradient / Alterações morfofisiológicas em mudas de Acca sellowiana (Myrtaceae: Myrtoideae) sob gradiente de sombreamento

Abstract Understanding morphological and physiological changes under different light conditions in native fruit species in juveniles' stage is important, as it indicate the appropriate environment to achieve vigorous saplings. We aimed to verify growth and morphophysiological changes under shade gradient in feijoa (Acca sellowiana (O. Berg) Burret) to achieve good quality saplings adequate to improve cultivation in orchards. The saplings were grown for twenty-one-month under four shading treatments (0%, 30%, 50%, and 80%). Growth, photosynthetic pigments, gas exchanges, chlorophyll fluorescence, and leaf anatomy parameters were evaluated. Saplings under full sun and 30% shade had higher height and diameter growth and dry mass accumulation due to higher photosynthesis rate. As main acclimatization mechanisms in feijoa saplings under 80% shade were developed larger leaf area, reduced leaf blade thickness, and enhanced quantum yield of photosystem II. Even so, the net CO2 assimilation and the electron transport rate was lower and, consequently, there was a restriction on the growth and dry mass in saplings under deep shade. Therefore, to obtain higher quality feijoa saplings, we recommend that it be carried out in full sun or up to 30% shade, to maximize the sapling vigor in nurseries and, later, this light environment can also be used in orchards for favor growth and fruit production.

Resumo A verificação de mudanças morfológicas e fisiológicas sob diferentes condições luminosas em espécies frutíferas nativas em estágio juvenil é importante, uma vez que indicam o ambiente adequado para a formação de mudas com alto vigor. Objetivou-se verificar o crescimento e as alterações morfofisiológicas sob gradiente de sombreamento em mudas de feijoa (Acca sellowiana (O. Berg) Burret) para obter mudas de boa qualidade, adequadas para fomentar os plantios da espécie em pomares. As mudas foram cultivadas por vinte e um meses sob quatro tratamentos de sombreamento (0%, 30%, 50% e 80%). Foram avaliados parâmetros de crescimento, pigmentos fotossintéticos, trocas gasosas, fluorescência da clorofila e anatomia foliar. Mudas a pleno sol e 30% de sombra apresentaram maior crescimento em altura, diâmetro e acúmulo de massa seca, devido à maior taxa de fotossíntese. Como principais mecanismos de aclimatação sob 80% de sombra, as mudas desenvolveram maior área foliar, redução da espessura do limbo foliar e aumento do rendimento quântico do fotossistema II. Mesmo assim, a assimilação líquida de CO2 e a taxa de transporte de elétrons foram menores e, consequentemente, houve restrição ao crescimento e acúmulo de massa seca das mudas no maior nível de sombreamento. Portanto, para a obtenção de mudas de feijoa de maior qualidade, recomendamos que seja realizada a pleno sol ou até 30% de sombra, para maximizar o vigor das mudas em viveiros e, posteriormente, este ambiente de luz também pode ser utilizado em pomares para favorecer o crescimento e a produção de frutos.

Morphophysiological changes of Acca sellowiana (Myrtaceae: Myrtoideae) saplings under shade gradient

Abstract Understanding morphological and physiological changes under different light conditions in native fruit species in juveniles stage is important, as it indicate the appropriate environment to achieve vigorous saplings. We aimed to verify growth and morphophysiological changes under shade gradient in feijoa (Acca sellowiana (O. Berg) Burret) to achieve good quality saplings adequate to improve cultivation in orchards. The saplings were grown for twenty-one-month under four shading treatments (0%, 30%, 50%, and 80%). Growth, photosynthetic pigments, gas exchanges, chlorophyll fluorescence, and leaf anatomy parameters were evaluated. Saplings under full sun and 30% shade had higher height and diameter growth and dry mass accumulation due to higher photosynthesis rate. As main acclimatization mechanisms in feijoa saplings under 80% shade were developed larger leaf area, reduced leaf blade thickness, and enhanced quantum yield of photosystem II. Even so, the net CO2 assimilation and the electron transport rate was lower and, consequently, there was a restriction on the growth and dry mass in saplings under deep shade. Therefore, to obtain higher quality feijoa saplings, we recommend that it be carried out in full sun or up to 30% shade, to maximize the sapling vigor in nurseries and, later, this light environment can also be used in orchards for favor growth and fruit production.

Resumo A verificação de mudanças morfológicas e fisiológicas sob diferentes condições luminosas em espécies frutíferas nativas em estágio juvenil é importante, uma vez que indicam o ambiente adequado para a formação de mudas com alto vigor. Objetivou-se verificar o crescimento e as alterações morfofisiológicas sob gradiente de sombreamento em mudas de feijoa (Acca sellowiana (O. Berg) Burret) para obter mudas de boa qualidade, adequadas para fomentar os plantios da espécie em pomares. As mudas foram cultivadas por vinte e um meses sob quatro tratamentos de sombreamento (0%, 30%, 50% e 80%). Foram avaliados parâmetros de crescimento, pigmentos fotossintéticos, trocas gasosas, fluorescência da clorofila e anatomia foliar. Mudas a pleno sol e 30% de sombra apresentaram maior crescimento em altura, diâmetro e acúmulo de massa seca, devido à maior taxa de fotossíntese. Como principais mecanismos de aclimatação sob 80% de sombra, as mudas desenvolveram maior área foliar, redução da espessura do limbo foliar e aumento do rendimento quântico do fotossistema II. Mesmo assim, a assimilação líquida de CO2 e a taxa de transporte de elétrons foram menores e, consequentemente, houve restrição ao crescimento e acúmulo de massa seca das mudas no maior nível de sombreamento. Portanto, para a obtenção de mudas de feijoa de maior qualidade, recomendamos que seja realizada a pleno sol ou até 30% de sombra, para maximizar o vigor das mudas em viveiros e, posteriormente, este ambiente de luz também pode ser utilizado em pomares para favorecer o crescimento e a produção de frutos.

Morphophysiological changes of Acca sellowiana (Myrtaceae: Myrtoideae) saplings under shade gradient.

Understanding morphological and physiological changes under different light conditions in native fruit species in juveniles' stage is important, as it indicate the appropriate environment to achieve vigorous saplings. We aimed to verify growth and morphophysiological changes under shade gradient in feijoa (Acca sellowiana (O. Berg) Burret) to achieve good quality saplings adequate to improve cultivation in orchards. The saplings were grown for twenty-one-month under four shading treatments (0%, 30%, 50%, and 80%). Growth, photosynthetic pigments, gas exchanges, chlorophyll fluorescence, and leaf anatomy parameters were evaluated. Saplings under full sun and 30% shade had higher height and diameter growth and dry mass accumulation due to higher photosynthesis rate. As main acclimatization mechanisms in feijoa saplings under 80% shade were developed larger leaf area, reduced leaf blade thickness, and enhanced quantum yield of photosystem II. Even so, the net CO2 assimilation and the electron transport rate was lower and, consequently, there was a restriction on the growth and dry mass in saplings under deep shade. Therefore, to obtain higher quality feijoa saplings, we recommend that it be carried out in full sun or up to 30% shade, to maximize the sapling vigor in nurseries and, later, this light environment can also be used in orchards for favor growth and fruit production.

Protective effects of the angiotensin type 1 receptor antagonist losartan in infection-induced and arthritis-associated alveolar bone loss.

BACKGROUND AND OBJECTIVE: The angiotensin type 1 (AT1) receptor has been implicated in the pathogenesis of inflammatory bone disorders. This study aimed to investigate the effect of an AT1 receptor antagonist in infection-induced and arthritis-associated alveolar bone loss in mice. MATERIAL AND METHODS: Mice were subjected to Aggregatibacter actinomycetemcomitans oral infection or antigen-induced arthritis and treated daily with 10 mg/kg of the prototype AT1 antagonist, losartan. Treatment was conducted for 30 d in the infectious condition and for 17 d and 11 d in the preventive or therapeutic regimens in the arthritic model, respectively. The mice were then killed, and the maxillae, serum and knee joints were collected for histomorphometric and immunoenzymatic assays. In vitro osteoclast assays were performed using RAW 264.7 cells stimulated with A. actinomycetemcomitans lipopolysacharide (LPS). RESULTS: Arthritis and A. actinomycetemcomitans infection triggered significant alveolar bone loss in mice and increased the levels of myeloperoxidase and of TRAP(+) osteoclasts in periodontal tissues. Losartan abolished such a phenotype, as well as the arthritis joint inflammation. Both arthritis and A. actinomycetemcomitans conditions were associated with the release of tumor necrosis factor alpha (TNF-α), interferon-gamma, interleukin-17 and chemokine (C-X-C motif) ligand 1 and an increased RANKL/osteoprotegerin ratio in periodontal tissues, but such expression decreased after losartan treatment, except for TNF-α. The therapeutic approach was as beneficial as the preventive one. In vitro, losartan prevented LPS-induced osteoclast differentiation and activity. CONCLUSION: The blockade of AT1 receptor exerts anti-inflammatory and anti-osteoclastic effects, thus protecting periodontal tissues in distinct pathophysiological conditions of alveolar bone loss.

The relevance of leukotrienes for bone resorption induced by mechanical loading.

5-Lipoxygenase (5-LO) metabolites are important pro-inflammatory lipid mediators. However, much still remains to be understood about the role of such mediators in bone remodeling. This study aimed to investigate the effect of 5-LO metabolites, LTB4 and CysLTs, in a model of mechanical loading-induced bone remodeling. Strain-induced tooth movement and consequently alveolar bone resorption/apposition was achieved by using a coil spring placed on molar and attached to incisors of C57BL6 (wild-type-WT), 5-LO deficient mice (5-LO(-/-)) and mice treated with 5-LO inhibitor (zileuton-ZN) or with antagonist of CysLTs receptor (montelukast-MT). The amount of bone resorption and the number of osteoclasts were determined morphometrically. The expression of inflammatory and bone remodeling markers in periodontium was analyzed by qPCR. Osteoclast differentiation and TNF-α production were evaluated in vitro using RAW 264.7 cells treated with LTB4 or LTD4. Bone resorption, TRAP(+) cells and expression of Tnfa, Il10 and Runx2 were significantly diminished in 5-LO(-/-), ZN- and MT-treated mice. The expression of Rank was also reduced in 5-LO(-/-) and MT-treated mice. Accordingly, LTB4 and LTD4 in association with RANKL promoted osteoclast differentiation and increased TNF-α release in vitro. These data demonstrate that the absence of 5-LO metabolites, LTB4 and CysLTs reduces osteoclast recruitment and differentiation, consequently diminishing bone resorption induced by mechanical loading. Thus, 5-LO might be a potential target for controlling bone resorption in physiological and pathological conditions.

Seletividade de inseticidas utilizados na cultura algodoeira a ovos e larvas de terceiro instar de Cycloneda sanguinea / Selectivity of insecticides used in the cotton crop to eggs and third-instar larvae of Cycloneda sanguinea

Objetivou-se avaliar a seletividade fisiológica de inseticidas utilizados em algodoeiro para ovos e larvas de terceiro instar de Cycloneda sanguinea (Linnaeus, 1763) (Coleoptera: Coccinellidae). Os produtos utilizados em g i.a. L-1 de água foram triflumurom 0,048 (Certero 480 SC), espinosade 0,24 (Tracer 480 SC), clorfenapir 1,2 (Pirate 240 SC), clotianidina 0,33 (Focus 500 PM) e imidaclopride 0,33 + ß-ciflutrina 0,042 (Connect 100 + 12,5 SC). Utilizou-se água destilada como tratamento testemunha. Tanto ovos quanto larvas foram distribuídos em placas de Petri e tratados com os inseticidas por meio de pulverização em torre de Potter. O delineamento experimental utilizado foi o inteiramente ao acaso, com cinco repetições e seis tratamentos constituídos pelos cinco inseticidas e pela testemunha, sendo que cada parcela foi composta por dez ovos ou dez larvas de terceiro instar. Avaliaram-se o período embrionário e a viabilidade de ovos tratados; sobrevivência e duração de larvas de terceiro instar; sobrevivência e duração dos estágios de desenvolvimento larval e pupal, razão sexual e o total de ovos colocados pelas fêmeas provenientes de ovos e larvas de terceiro instar tratados. Os bioensaios foram conduzidos a 25 ± 2º C, UR 60 ± 10% e fotofase de 12h. O inseticida triflumurom 0,048 foi seletivo a ovos do predador e levemente nocivo a larvas de terceiro instar. Espinosade 0,24 foi levemente nocivo a ovos e larvas de terceiro instar, e clorfenapir 1,2; clotianidina 0,33 e imidaclopride 0,33 + ß-ciflutrina 0,042 foram prejudiciais a ovos e larvas de terceiro instar de C. sanguinea.

SELECTIVITY OF INSECTICIDES USED IN THE COTTON CROP TO EGGS AND THIRD-INSTAR LARVAE OF CYCLONEDA SANGUINEA. The objective of this work was to evaluate the effects of synthetic insecticides used in the cotton crop on eggs and third-instar larvae of the ladybeetle Cycloneda sanguinea (Linnaeus, 1763) (Coleoptera: Coccinellidae). The products used in g a.i. L-1 of water were triflumuron 0.048 (Certero 480 SC), spinosad 0.24 (Tracer 480 SC), chlorfenapyr 1.2 (Pirate 240 SC), chlothianidin 0.33 (Focus 500 WP) and imidacloprid 0.33 + ß-cyfluthrin 0.042 (Connect 100 + 12.5 SC). Distilled water was used as a control. Both eggs and larvae were distributed in Petri dishes and treated with the insecticides by spraying in a Potter’s tower. A fully randomized experimental design, with five replications and six treatments consisting of five pesticides and one control was used. Each plot consisted of ten eggs or third-instar larvae. The embryonic period and viability of the treated eggs; survival and duration of third-instar larvae; survival and duration of larval and pupal stages of development; sexual ratio, and the total number of eggs laid by the surviving mated females from treated eggs and third-instar larvae were evaluated. The tests were carried out at 25 ± 2º C, RH 60 ± 10% and 12h-photophase. Triflumuron 0.048 was innocuous to the predator’s eggs and slightly harmful to third-instar larvae. Spinosad 0.24 was slightly harmful to eggs and third-instar larvae, whereas chlofernapyr 1.2, chlothianidin 0.33 and imidacloprid 0.33 + ß-cyfluthrin 0.042 were harmful to eggs and third-instar larvae of C. sanguinea.

Seletividade de inseticidas utilizados na cultura algodoeira a ovos e larvas de terceiro instar de Cycloneda sanguinea / Selectivity of insecticides used in the cotton crop to eggs and third-instar larvae of Cycloneda sanguinea

Objetivou-se avaliar a seletividade fisiológica de inseticidas utilizados em algodoeiro para ovos e larvas de terceiro instar de Cycloneda sanguinea (Linnaeus, 1763) (Coleoptera: Coccinellidae). Os produtos utilizados em g i.a. L-1 de água foram triflumurom 0,048 (Certero 480 SC), espinosade 0,24 (Tracer 480 SC), clorfenapir 1,2 (Pirate 240 SC), clotianidina 0,33 (Focus 500 PM) e imidaclopride 0,33 + ß-ciflutrina 0,042 (Connect 100 + 12,5 SC). Utilizou-se água destilada como tratamento testemunha. Tanto ovos quanto larvas foram distribuídos em placas de Petri e tratados com os inseticidas por meio de pulverização em torre de Potter. O delineamento experimental utilizado foi o inteiramente ao acaso, com cinco repetições e seis tratamentos constituídos pelos cinco inseticidas e pela testemunha, sendo que cada parcela foi composta por dez ovos ou dez larvas de terceiro instar. Avaliaram-se o período embrionário e a viabilidade de ovos tratados; sobrevivência e duração de larvas de terceiro instar; sobrevivência e duração dos estágios de desenvolvimento larval e pupal, razão sexual e o total de ovos colocados pelas fêmeas provenientes de ovos e larvas de terceiro instar tratados. Os bioensaios foram conduzidos a 25 ± 2º C, UR 60 ± 10% e fotofase de 12h. O inseticida triflumurom 0,048 foi seletivo a ovos do predador e levemente nocivo a larvas de terceiro instar. Espinosade 0,24 foi levemente nocivo a ovos e larvas de terceiro instar, e clorfenapir 1,2; clotianidina 0,33 e imidaclopride 0,33 + ß-ciflutrina 0,042 foram prejudiciais a ovos e larvas de terceiro instar de C. sanguinea. (AU)

SELECTIVITY OF INSECTICIDES USED IN THE COTTON CROP TO EGGS AND THIRD-INSTAR LARVAE OF CYCLONEDA SANGUINEA. The objective of this work was to evaluate the effects of synthetic insecticides used in the cotton crop on eggs and third-instar larvae of the ladybeetle Cycloneda sanguinea (Linnaeus, 1763) (Coleoptera: Coccinellidae). The products used in g a.i. L-1 of water were triflumuron 0.048 (Certero 480 SC), spinosad 0.24 (Tracer 480 SC), chlorfenapyr 1.2 (Pirate 240 SC), chlothianidin 0.33 (Focus 500 WP) and imidacloprid 0.33 + ß-cyfluthrin 0.042 (Connect 100 + 12.5 SC). Distilled water was used as a control. Both eggs and larvae were distributed in Petri dishes and treated with the insecticides by spraying in a Potters tower. A fully randomized experimental design, with five replications and six treatments consisting of five pesticides and one control was used. Each plot consisted of ten eggs or third-instar larvae. The embryonic period and viability of the treated eggs; survival and duration of third-instar larvae; survival and duration of larval and pupal stages of development; sexual ratio, and the total number of eggs laid by the surviving mated females from treated eggs and third-instar larvae were evaluated. The tests were carried out at 25 ± 2º C, RH 60 ± 10% and 12h-photophase. Triflumuron 0.048 was innocuous to the predators eggs and slightly harmful to third-instar larvae. Spinosad 0.24 was slightly harmful to eggs and third-instar larvae, whereas chlofernapyr 1.2, chlothianidin 0.33 and imidacloprid 0.33 + ß-cyfluthrin 0.042 were harmful to eggs and third-instar larvae of C. sanguinea. (AU)

Seletividade de inseticidas utilizados na cultura algodoeira a ovos e larvas de terceiro instar de Cycloneda sanguinea

The objective of this work was to evaluate the effects of synthetic insecticides used in the cotton crop on eggs and third-instar larvae of the ladybeetle Cycloneda sanguinea (Linnaeus, 1763) (Coleoptera: Coccinellidae). The products used in g a.i. L-1 of water were triflumuron 0.048 (Certero 480 SC), spinosad 0.24 (Tracer 480 SC), chlorfenapyr 1.2 (Pirate 240 SC), chlothianidin 0.33 (Focus 500 WP) and imidacloprid 0.33 + -cyfluthrin 0.042 (Connect 100 + 12.5 SC). Distilled water was used as a control. Both eggs and larvae were distributed in Petri dishes and treated with the insecticides by spraying in a Potter's tower. A fully randomized experimental design, with five replications and six treatments consisting of five pesticides and one control was used. Each plot consisted of ten eggs or third-instar larvae. The embryonic period and viability of the treated eggs; survival and duration of third-instar larvae; survival and duration of larval and pupal stages of development; sexual ratio, and the total number of eggs laid by the surviving mated females from treated eggs and third-instar larvae were evaluated. The tests were carried out at 25 ± 2º C, RH 60 ± 10% and 12h-photophase. Triflumuron 0.048 was innocuous to the predator's eggs and slightly harmful to third-instar larvae. Spinosad 0.24 was slightly harmful to eggs and third-instar larvae, whereas chlofernapyr 1.2, chlothianidin 0.33 and imidacloprid 0.33 + -cyfluthrin 0.042 were harmful to eggs and third-instar larvae of C. sanguinea.

Objetivou-se avaliar a seletividade fisiológica de inseticidas utilizados em algodoeiro para ovos e larvas de terceiro instar de Cycloneda sanguinea (Linnaeus, 1763) (Coleoptera: Coccinellidae). Os produtos utilizados em g i.a. L-1 de água foram triflumurom 0,048 (Certero 480 SC), espinosade 0,24 (Tracer 480 SC), clorfenapir 1,2 (Pirate 240 SC), clotianidina 0,33 (Focus 500 PM) e imidaclopride 0,33 + -ciflutrina 0,042 (Connect 100 + 12,5 SC). Utilizou-se água destilada como tratamento testemunha. Tanto ovos quanto larvas foram distribuídos em placas de Petri e tratados com os inseticidas por meio de pulverização em torre de Potter. O delineamento experimental utilizado foi o inteiramente ao acaso, com cinco repetições e seis tratamentos constituídos pelos cinco inseticidas e pela testemunha, sendo que cada parcela foi composta por dez ovos ou dez larvas de terceiro instar. Avaliaram-se o período embrionário e a viabilidade de ovos tratados; sobrevivência e duração de larvas de terceiro instar; sobrevivência e duração dos estágios de desenvolvimento larval e pupal, razão sexual e o total de ovos colocados pelas fêmeas provenientes de ovos e larvas de terceiro instar tratados. Os bioensaios foram conduzidos a 25 ± 2º C, UR 60 ± 10% e fotofase de 12h. O inseticida triflumurom 0,048 foi seletivo a ovos do predador e levemente nocivo a larvas de terceiro instar. Espinosade 0,24 foi levemente nocivo a ovos e larvas de terceiro instar, e clorfenapir 1,2; clotianidina 0,33 e imidaclopride 0,33 + -ciflutrina 0,042 foram prejudiciais a ovos e larvas de terceiro instar de C. sanguinea.

Levantamento e flutuação populacional de parasitoides de moscasdas-frutas (Diptera: Tephritidae) de ocorrência em goiabeira (Psidium guajava L) em Fortaleza, Ceará / Survey and populational dynamics of parasitoids of fruit flies (Diptera: Tephritidae) occuring in guava (Psidium guajava L) in Fortaleza, Ceará, Brazil

Objetivou-se com o presente trabalho conhecer os himenópteros parasitoides associados às moscas-das-frutas (Diptera: Tephritidae) de ocorrência em pomar urbano de goiabeira (Psidium guajava L.) (Myrtaceae), em Fortaleza, Ceará, bem como determinar a flutuação populacional desses organismos nesse ecossistema. Realizaram-se coletas de frutos maduros em plantas de goiabeira e frutos recém-caídos, no período de novembro de 1999 a outubro de 2000. Os parasitoides obtidos pertencem às famílias Braconidae, Chalcididae, Figitidae (Eucoilinae), Diapriidae (Diapriinae) e Eulophidae, sendo que os da família Figitidae são os mais comuns. As maiores incidências desses organismos ocorreram nos meses de janeiro e março de 2000.

This study was aimed to identify the parasitoids associated to fruit-fly species (Diptera: Tephritidae) occurring in an urban guava (Psidium guajava L.) (Myrtaceae) orchard located in Fortaleza, Ceará State, Brazil, as well as to determine the populational dynamics of these organ isms in the ecosystem. Ripe and newly fallen guava fruits, from November 1999 to December 2000 were collected. The parasitoids obtained belong to the families Braconidae, Chalcididae, Figitidae (Eucoilinae), Diapriidae (Diapriinae) and Eulophidae. The most common parasitoids belong to the family Figitidae. The highest occurrences were in January and March 2000.

LEVANTAMENTO E FLUTUAÇÃO POPULACIONAL DE PARASITOIDES DE MOSCASDAS-FRUTAS (DIPTERA: TEPHRITIDAE) DE OCORRÊNCIA EM GOIABEIRA (PSIDIUM GUAJAVA L.) EM FORTALEZA, CEARÁ

ABSTRACT This study was aimed to identify the parasitoids associated to fruit-fly species (Diptera: Tephritidae) occurring in an urban guava (Psidium guajava L.) (Myrtaceae) orchard located in Fortaleza, Ceará State, Brazil, as well as to determine the populational dynamics of these organ isms in the ecosystem. Ripe and newly fallen guava fruits, from November 1999 to December 2000 were collected. The parasitoids obtained belong to the families Braconidae, Chalcididae, Figitidae (Eucoilinae), Diapriidae (Diapriinae) and Eulophidae. The most common parasitoids belong to the family Figitidae. The highest occurrences were in January and March 2000.

RESUMO Objetivou-se com o presente trabalho conhecer os himenópteros parasitoides associados às moscas-das-frutas (Diptera: Tephritidae) de ocorrência em pomar urbano de goiabeira (Psidium guajava L.) (Myrtaceae), em Fortaleza, Ceará, bem como determinar a flutuação populacional desses organismos nesse ecossistema. Realizaram-se coletas de frutos maduros em plantas de goiabeira e frutos recém-caídos, no período de novembro de 1999 a outubro de 2000. Os parasitoides obtidos pertencem às famílias Braconidae, Chalcididae, Figitidae (Eucoilinae), Diapriidae (Diapriinae) e Eulophidae, sendo que os da família Figitidae são os mais comuns. As maiores incidências desses organismos ocorreram nos meses de janeiro e março de 2000.

Preparation, characterization, and spectroscopic properties of PC/PMMA doped blends: study of the effect of rare-earth doping on luminescence, quenching rate, and lifetime enhancement.

Optical properties of highly transparent blends formed by bisphenol A polycarbonate (PC) and poly(methyl methacrylate) (PMMA) and doped with either europium(III) thenoyltrifluoroacetone hydrate [Eu(TTA)(3)-hydrate] or terbium(III) acetylacetonate hydrate [Tb(ACAC)(3)-hydrate] were studied. From the absorption/emission spectra (200 to 800 nm), percolation limits of less than 0.4 wt/wt% for Eu and above 0.7 wt/wt% for Tb were found. Lifetimes for 20/80 and 60/40 Tb-doped blends were from 1.0 to 1.5 ms, respectively, about 5 times longer than for a similar blend prepared with Langmuir-Blodgett film. For blends doped with Eu, the lifetimes were from 0.4 to 0.8 ms, almost double that for the pure complex. The results indicated interactions between the PC/PMMA blends and the rare-earth ion complex. Particularly, photoluminescent spectra evidenced the incorporation of the Eu(3+) and Tb(3+) ions into the blend, with quenching observed near 1.0 wt/wt%, except for the 20/80 blend doped with Eu ion, in which luminescence increased to 5 wt/wt %.

Seletividade fisiológica de inseticidas utilizados em cultura cafeeira sobre ovos e adultos de Cryptolaemus montrouzieri Mulsant / Physiologic selectivity of pesticides used on coffee plantations on eggs and adults of Cryptolaemus montrouzieri Mulsant

Em cafeeiros, a associação de inimigos naturais com produtos fitossanitários seletivos é uma importante estratégia no manejo de pragas. O objetivo do presente estudo foi avaliar os efeitos letais e subletais de produtos fitossanitários utilizados na cultura cafeeira sobre ovos e adultos de Cryptolaemus montrouzieriMulsant em laboratório. Os bioensaios foram realizados sob 25 ± 2º C, UR de 70 ± 10% e fotofase de 12h. Os inseticidas utilizados e suas respectivas dosagens de aplicação, em g ou mL de i.a. L-1 foram: tiametoxam (0,5), imidacloprido (0,7), óleo mineral (13,3), endossulfam (2,63) e dimetoato (0,48). A testemunha foi composta apenas por água destilada. A aplicação dos produtos foi realizada por meio de torre de Potter. Avaliaram-se a viabilidade de ovos, a sobrevivência dos espécimes e os efeitos dos compostos sobre os parâmetros reprodutivos do predador. Tiametoxam provocou prolongamento na duração do período embrionário (7,1 dias) em ovos tratados e junto com imidacloprido foram classificados como nocivos, seguidos do endossulfam e dimetoato que apresentaram toxicidade intermediária e do óleo mineral que foi seletivo. Em função da seletividade do óleo mineral, pode-se recomendá-lo visando à compatibilização com o predador C. montrouzieri em programas de manejo integrado de pragas na cultura cafeeira.

On coffee plantations, the association between selective compounds and natural enemies composes an important tool for the pest management. The objective of this study was to evaluate some pesticides used on coffee plantations in regard to their lethal and sublethal effects on eggs and adults of Cryptolaemus montrouzieri Mulsant under laboratory conditions. The bioassays were carried out in the Laboratory of Selectivity Studies, Department of Entomology of the Universidade Federal de Lavras ­ UFLA, Brazil, under controlled conditions (climatic chamber) at 25 ± 2º C, RH of 70 ± 10% with a 12 h photophase. The pesticides and doses in g or mL of i.a. L-1 were: thiamethoxam (0.5), imidacloprid (0.7), mineral oil (13.3), endosulfan (2.63) and dimethoate (0.48). Distilled water was used as a control. The sprayings of the pesticides were accomplished using a Potter's tower. The parameters evaluated were: specimen survival after the application of the compounds, oviposition and eggs viability. Thiamethoxam, imidacloprid and endosulfan were the most harmful to the treated individuals. When sprayed on eggs, thiamethoxam increased the embryonic period length (7.1 days). Mineral oil was the only compound that did not affect the reproduction of C. montrouzieri. In function of the selectivity presented by mineral oil, it can be recommended to be used in integrated pest management on coffee plantations in association with this predator.

SELETIVIDADE FISIOLÓGICA DE INSETICIDAS UTILIZADOS EM CULTURA CAFEEIRA SOBRE OVOS E ADULTOS DE CRYPTOLAEMUS MONTROUZIERI MULSANT

ABSTRACT On coffee plantations, the association between selective compounds and natural enemies composes an important tool for the pest management. The objective of this study was to evaluate some pesticides used on coffee plantations in regard to their lethal and sublethal effects on eggs and adults of Cryptolaemus montrouzieri Mulsant under laboratory conditions. The bioassays were carried out in the Laboratory of Selectivity Studies, Department of Entomology of the Universidade Federal de Lavras UFLA, Brazil, under controlled conditions (climatic chamber) at 25 ± 2º C, RH of 70 ± 10% with a 12 h photophase. The pesticides and doses in g or mL of i.a. L-1 were: thiamethoxam (0.5), imidacloprid (0.7), mineral oil (13.3), endosulfan (2.63) and dimethoate (0.48). Distilled water was used as a control. The sprayings of the pesticides were accomplished using a Potters tower. The parameters evaluated were: specimen survival after the application of the compounds, oviposition and eggs viability. Thiamethoxam, imidacloprid and endosulfan were the most harmful to the treated individuals. When sprayed on eggs, thiamethoxam increased the embryonic period length (7.1 days). Mineral oil was the only compound that did not affect the reproduction of C. montrouzieri. In function of the selectivity presented by mineral oil, it can be recommended to be used in integrated pest management on coffee plantations in association with this predator.

RESUMO Em cafeeiros, a associação de inimigos naturais com produtos fitossanitários seletivos é uma importante estratégia no manejo de pragas. O objetivo do presente estudo foi avaliar os efeitos letais e subletais de produtos fitossanitários utilizados na cultura cafeeira sobre ovos e adultos de Cryptolaemus montrouzieriMulsant em laboratório. Os bioensaios foram realizados sob 25 ± 2º C, UR de 70 ± 10% e fotofase de 12h. Os inseticidas utilizados e suas respectivas dosagens de aplicação, em g ou mL de i.a. L-1 foram: tiametoxam (0,5), imidacloprido (0,7), óleo mineral (13,3), endossulfam (2,63) e dimetoato (0,48). A testemunha foi composta apenas por água destilada. A aplicação dos produtos foi realizada por meio de torre de Potter. Avaliaram-se a viabilidade de ovos, a sobrevivência dos espécimes e os efeitos dos compostos sobre os parâmetros reprodutivos do predador. Tiametoxam provocou prolongamento na duração do período embrionário (7,1 dias) em ovos tratados e junto com imidacloprido foram classificados como nocivos, seguidos do endossulfam e dimetoato que apresentaram toxicidade intermediária e do óleo mineral que foi seletivo. Em função da seletividade do óleo mineral, pode-se recomendá-lo visando à compatibilização com o predador C. montrouzieri em programas de manejo integrado de pragas na cultura cafeeira.

Chaotic advection in blood flow.

In this paper we argue that the effects of irregular chaotic motion of particles transported by blood can play a major role in the development of serious circulatory diseases. Vessel wall irregularities modify the flow field, changing in a nontrivial way the transport and activation of biochemically active particles. We argue that blood particle transport is often chaotic in realistic physiological conditions. We also argue that this chaotic behavior of the flow has crucial consequences for the dynamics of important processes in the blood, such as the activation of platelets which are involved in the thrombus formation.

Toxicidade de óleo de nim para pupas e adultos de chrysoperla externa (hagen) (neuroptera: chrysopidae)

ABSTRACT The objective of this work was to evaluate the effects of Nim-I-Go on pupae and adults of Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae). The neem-based insecticide Nim-I-Go was used in the following concentrations (%) in the pupae trial: 0.5; 1.0; 2.0 and 5.0; equivalent to (in mg of azadirachtin/L of water) 5 mg/L, 10 mg/L, 20 mg/L and 50 mg/L, respectively. In the larvae trial the following concentrations were used: 0.25; 0.5; 0.75; 1.0 and 2.0; equivalent to (in mg of azadirachtin/L) 2.5 mg/L, 5.0 mg/L, 7.5 mg/L, 10 mg/L and 20 mg/L, respectively. Chlorpyrifos was used as a negative control (0.48 g a.i./L) in both trials, and water as a positive control. The bioassays were carried out at 25 ± 2o C, RH of 70 ± 10%, and 12-h of photophase. The sprayings were carried out using a Potters tower, ensuring the application of 1.5 ± 0.5 mg/cm2. Survival of pupae and adults, and the effects of the insecticides on the reproductive capacity of this predator were evaluated. The total toxic effect (E) of the insecticides was calculated according to the standard methodology established by IOBC. Nim-I-Go was harmless to pupae and adults of C. externa. Chlorpyrifos was harmful to this natural enemy.

RESUMO O objetivo do presente trabalho foi avaliar os efeitos do Nim-I-Go® sobre pupas e adultos de Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae). As concentrações (%) do Nim-I-Go® utilizadas no experimento com pupas foram as seguintes: 0,5; 1,0; 2,0 e 5,0; correspondendo a (mg de azadirachtina/L de água) 5 mg/L, 10 mg/L, 20 mg/L e 50 mg/L, respectivamente. No experimento com adultos foram utilizadas as seguintes concentrações: 0,25; 0,5; 0,75; 1,0 e 2,0; correspondendo a (mg de azadirachtina/L) 2,5 mg/L, 5,0 mg/L, 7,5 mg/L, 10 mg/L e 20 mg/L, respectivamente. Clorpirifós foi utilizado como testemunha negativa (0,48 g i.a./L) nos dois experimentos e água como testemunha positiva. Os bioensaios foram conduzidos a 25 ± 2 o C, UR de 70 ± 10% e fotofase de 12 horas. As pulverizações foram realizadas por meio de torre de Potter com aplicação de 1,5 ± 0,5 mg de calda química/cm2. Avaliaram-se a viabilidade dos ovos, a sobrevivência de pupas e adultos e o efeito dos inseticidas sobre a capacidade reprodutiva dos indivíduos. A toxicidade foi calculada em função do efeito total (E), conforme metodologia padrão estabelecida pela IOBC. Nim-I-Go® nas diversas concentrações foi inócuo a pupas e adultos do predador. Clorpirifós foi nocivo para pupas e adultos de C. externa.

Toward an understanding of intermediate- and short-range defects in ZnO single crystals. A combined experimental and theoretical study.

A joint use of experimental and theoretical techniques allows us to understand the key role of intermediate- and short-range defects in the structural and electronic properties of ZnO single crystals obtained by means of both conventional hydrothermal and microwave-hydrothermal synthesis methods. X-ray diffraction, Raman spectra, photoluminescence, scanning electronic and transmission electron microscopies were used to characterize the thermal properties, crystalline and optical features of the obtained nano and microwires ZnO structures. In addition, these properties were further investigated by means of two periodic models, crystalline and disordered ZnO wurtzite structure, and first principles calculations based on density functional theory at the B3LYP level. The theoretical results indicate that the key factor controlling the electronic behavior can be associated with a symmetry breaking process, creating localized electronic levels above the valence band.

Ocorrência de aceria guerreronis keifer e steneotarsonemus furcatus de leon (acari: eriophyidae; tarsonemidae) em coqueiro na região do vale do rio doce

ABSTRACT This work was carried out to identify the mite species on coconut fruits (Cocus nucifera) (Arecaceae) in the Vale do Rio Doce region, eastern Minas Gerais State, Brazil. Sampling of fruits was made from March of 2002 to October of 2003, on 42 farms. Two species of mites were found: Aceria guerreronis Keifer (Eriophyidae) and Steneotarsonemus furcatus De Leon (Tarsonemidae). This is the first report of A. guerreironis and S. furcatus in the Vale do Rio Doce region.

RESUMO O presente trabalho teve por objetivo identificar as espécies de ácaro encontradas em frutos de coqueiro (Cocus nucifera) (Arecaceae) na região do Vale do Rio Doce, leste do Estado de Minas Gerais. As coletas de frutos de coqueiro foram realizadas no período de março de 2002 a outubro de 2003, em 42 propriedades agrícolas. Duas espécies de ácaro foram registradas: Aceria guerreronis Keifer (Acari: Eriophyidae) e Steneotarsonemus furcatus De Leon (Acari: Tarsonemidae). Este é o primeiro registro de A. guerreronis e S. furcatus na Região do Vale do Rio Doce.

Efeitos de inseticidas botânico e sintéticos sobre ovos e larvas de cycloneda sanguinea (linnaeus) (coleoptera: coccinellidae) em condições de laboratório

ABSTRACT The objective of this work was to evaluate the effects of azadirachtin and some synthetic insecticides used in the cotton crops on eggs and firstand fourth-instar larvae of Cycloneda sanguinea (Linnaeus). The neem-based insecticide Nim-I-Go was used in the following concentrations: 1% (10 mg of azadirachtin/L), 5% (50 mg of azadirachtin/L) and 10% (100 mg of azadirachtin/L). The synthetic insecticides teflubenzuron (Nomolt 150 SC) (0.15 g a.i./ L), lambdacyhalothrin (Karate Zeon 250 CS (0.08 g a.i./L) and chlorpyrifos (Vexter 480 CE) (1.6 g a.i./L) also were used in this study. Distilled water was used as control. A fully randomized experimental design was used, with seven treatments and six replicates, each one formed of three eggs or larvae of C. sanguinea. A manual sprayer was used to spew the solutions. The bioassays were kept in a room at 25±2oC, RH of 70±10% and 12h photophase. The insects were fed with Schizaphis graminum (Rondani). Lambdacyhalothrin and chlorpyrifos were harmful to all stages of C. sanguinea. Azadirachtin at 50 and 100 mg;L was toxic to fourth-instar larvae of C. sanguinea. Based in its low toxicity, azadirachtin 10 mg/L and teflubenzuron present favorable characteristics for use in the integrated pest management where C. sanguinea is an important factor of natural mortality of pests.

RESUMO Este trabalho objetivou avaliar os efeitos do azadirachtina e de alguns inseticidas sintéticos utilizados na cultura do algodoeiro sobre ovos e larvas de primeiro e quarto instares do predador Cycloneda sanguinea (Linnaeus). Nim-I-Go® (azadirachtina) foi utilizado nas concentrações de 1%, 5% e 10%, correspondendo a 10 mg/L, 50 mg/L e 100 mg/L de azadirachtina, respectivamente. Os inseticidas utilizados foram teflubenzurom (Nomolt 150 SC) (0,15 g i.a./L), lambdacialotrina (Karate Zeon 250 CS) (0,08 g i.a./L) e clorpirifós (Vexter 480 CE) (1,6 g i.a./L). O tratamento testemunha foi composto somente por água destilada. Utilizaram-se sete tratamentos com seis repetições, sendo cada parcela formada por três ovos ou larvas de C. sanguinea, sendo as aplicações realizadas com um pulverizador manual. Os bioensaios foram mantidos a 25 ± 2o C, UR de 70 ± 10% e fotofase de 12h. Os insetos foram alimentados ad libitum com o pulgão-verde-do-sorgo Schizaphis graminum (Rondani). Todos o compostos avaliados reduziram a viabilidade de ovos. Lambdacialotrina e clorpirifós foram altamente tóxicos para larvas de 1 o e 4o instar de C. sanguinea. Azadirachtina a 50 e 100 mg/L foi tóxico para larvas de quarto instar de C. sanguinea. Em função da baixa toxicidade apresentada, azadirachtina 10 mg/L e teflubenzuron apresentam as características mais favoráveis para uso em associação com C. sanguinea, para o controle de pragas na cultura algodoeira, dentre os compostos testados.

Pesquisa de coliformes termotolerantes, totais e salmonella spp. em carnes caprinas comercializadas na cidade do recife, pernambuco

ABSTRACT The present study was aimed to evaluate the contamination by thermotolerant and total coliforms of in natura andunfrozencaprinemeatcommercializedinthemarketsofthecityof Recife, Pernambuco, Brazil, while also identifying, typifying and evaluating the profile of in-vitro susceptibility of the samples of Salmonella spp. Twenty-four samples were analyzed, using official methodologies established by the Animal Laboratory Coordination of the Ministry of Agriculture, Livestock and Supply. The results obtained demonstrate that 15 (62.50%) of the samples analyzed were not within the microbiologic standards. As for total coliforms, 5 (20.83%) samples were found to be in satisfactory sanitary conditions and 19 (79.16%) were found to be outside the meat standard. In the Salmonella spp. research, 7 (29.17%) samples presented contamination by Salmonella spp., there being 8 sorotypes typified: 4 (50%) Salmonella enterica subsp. houtena (O:53), 2 (25%), S. Anatum, 1 (12.50%) S. Rubislaw, 1 (12.50%) S. Derby. The profile of susceptibility obtained in vitro was 100% for norfloxacine, 75% for sulpha + trimetoprim, 62.50% for tetracycline, with a variation of 100% in the resistance to penicillin, novobiocin and oxaciline and of 87.50% for amoxiciline. The study showed that there is a high rate of contamination by thermotolerant and by total coliforms, besides confirming the presence of Salmonella spp. in caprine meat commercialized in the markets of the city of the Recife, constituting a risk factor for public health. Intervention by the departments

RESUMO Objetivou-se com este trabalho avaliar a contaminação na carne caprina in natura e resfriada comercializada nos mercados da Cidade do Recife, PE, por coliformes termotolerantes e totais, além de identificar, tipificar e avaliar o perfil de sensibilidade in vitro das amostras de Salmonella spp. Foram analisadas 24 amostras, utilizando-se metodologias oficiais preconizadas pela Coordenação de Laboratório Animal do Ministério da Agricultura, Pecuária e Abastecimento. Os resultados obtidos demonstram que 15 (62,50%) amostras analisadas encontram-se fora dos padrões microbiológicos. Em relação aos coliformes totais, 5 (20,83%) amostras encontravam-se em condições sanitárias satisfatórias e 19 (79,16%) encontravam-se fora dos padrões para carnes. Na pesquisa de Salmonella spp., 7 (29,17%) amostras apresentaram contaminação por Salmonella spp., sendo tipificados 8 sorotipos: 4 (50%) Salmonella enterica subsp. houtenae (O:53), 2 (25%), S. Anatum, 1 (12,50%) S. Rubislaw, 1 (12,50%) S. Derby. O perfil de sensibilidade in vitro obtido foi de 100% para norfloxacina, 75% para sulfa + trimetoprim, 62,50% para tetraciclina, com variação de 100 na resistência à penicilina, novobiocina e oxacilina e de 87,50% para amoxicilina. Conclui-se que existe um elevado índice de contaminação por coliformes totais e termotolerantes, além de confirmar a presença de Salmonella spp. em carnes caprinas comercializadas nos mercados da Cidade do Recife, PE, constituindo um fator de risco para a saúde pública. A intervenção dos órgãos de Vigilância Sanitária é necessária para melhorar as condições de produção, manuseio e de venda deste produto, além de estabelecer padrões microbiológicos para coliformes totais e termotolerantes para carne caprina resfriada, congelada ou in natura.