ABSTRACT
The study aimed to evaluate the antitumor and toxicogenetic effects of liposomal nanoformulations containing citrinin in animal breast carcinoma induced by 7,12-dimethylbenzanthracene (DMBA). Mus musculus virgin females were divided into six groups treated with (1) olive oil (10 mL/kg); (2) 7,12-DMBA (6 mg/kg); (3) citrinin, CIT (2 mg/kg), (4) cyclophosphamide, CPA (25 mg/kg), (5) liposomal citrinin, LP-CIT (2 µg/kg), and (6) LP-CIT (6 µg/kg). Metabolic, behavioral, hematological, biochemical, histopathological, and toxicogenetic tests were performed. DMBA and cyclophosphamide induced behavioral changes, not observed for free and liposomal citrinin. No hematological or biochemical changes were observed for LP-CIT. However, free citrinin reduced monocytes and caused hepatotoxicity. During treatment, significant differences were observed regarding the weight of the right and left breasts treated with DMBA compared to negative controls. Treatment with CPA, CIT, and LP-CIT reduced the weight of both breasts, with better results for liposomal citrinin. Furthermore, CPA, CIT, and LP-CIT presented genotoxic effects for tumor, blood, bone marrow, and liver cells, although less DNA damage was observed for LP-CIT compared to CIT and CPA. Healthy cell damage induced by LP-CIT was repaired during treatment, unlike CPA, which caused clastogenic effects. Thus, LP-CIT showed advantages for its use as a model of nanosystems for antitumor studies.
ABSTRACT
Introdução: o plasma rico em plaquetas é definido como um concentrado de plaquetas autólogas, obtido por centrifugação de sangue total. Trata-se de uma técnica inovadora, simples e de baixo custo, que apresenta muitos benefícios, podendo ser aplicada em diferentes áreas da saúde. Objetivo: a presente pesquisa buscou desenvolver um protocolo operacional padrão para obtenção e aplicação do plasma sanguíneo rico em plaquetas para o tratamento de alterações estéticas faciais. Metodologia: o estudo tratou de uma pesquisa de campo experimental, de caráter qualitativo e quantitativo. Um processo seletivo foi realizado para recrutar pacientes voluntários para o estudo, que foram selecionados de acordo com os critérios de inclusão e exclusão. Através de testes foi possível chegar ao tempo de centrifugação e velocidade ideais para a produção do plasma rico em plaquetas e testá-lo no tratamento de alterações estéticas faciais. Resultados e discussão: as principais mudanças que foram notadas pelos pacientes durante e após o tratamento foram a redução das cicatrizes de acne e aumento da hidratação e viscosidade da pele. A melhora das alterações estéticas tratadas foi notada por 100% dos pacientes, não havendo piora em nenhum paciente. Conclusão: com base nos testes e resultados obtidos, foi possível padronizar um protocolo operacional padrão ideal para a obtenção e aplicação do plasma rico em plaquetas, comprovando sua eficácia no tratamento de alterações estéticas faciais como rugas, flacidez, linhas de expressões acentuadas, acne, cicatrizes de acne, além de ter-se observado uma melhora significativa na hidratação da pele e redução de poros dilatados.
Introduction: platelet-rich plasma is defined as a concentrate of autologous platelets, obtained by centrifuging whole blood. It is an innovative, simple and low-cost technique that has many benefits, and can be applied in different areas of health. Objective: the present research sought to develop a standard operating protocol for obtaining and applying platelet-rich blood plasma for the treatment of facial aesthetic changes. Metodology: the study was a qualitative and quantitative field research. A selection process was carried out to recruit volunteer patients for the study, who were selected according to the inclusion and exclusion criteria. Through tests it was possible to reach the ideal centrifugation time and speed for the production of platelet-rich plasma and to test it in the treatment of facial aesthetic changes. Results and discussion: the main changes that were noted by patients during and after treatment were the reduction of acne scars and increased hydration and skin viscosity. The improvement of the aesthetic changes treated was noticed by 100% of the patients, with no worsening in any patient. Conclusion: based on the tests and results obtained, it was possible to standardize an ideal standard operating protocol for obtaining and applying platelet-rich plasma, proving its effectiveness in the treatment of facial aesthetic changes such as wrinkles, sagging, accentuated expression lines, acne, scars from acne, in addition to a significant improvement in skin hydration and reduction of enlarged pores.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Therapeutics , Intercellular Signaling Peptides and Proteins , Esthetics , Platelet-Rich Plasma , Cicatrix , Acne Vulgaris , Evaluation Studies as TopicABSTRACT
BACKGROUND: Cardiovascular diseases are a group of disorders of the heart and blood vessels that account for a major cause of premature death worldwide. Diosgenin saponin is a steroid for therapeutic application in different clinical settings such as cardiovascular diseases, hyperglycemia and dyslipidemia control, antimicrobial, anti-viral and anti-inflammatory agent. The aim of the study was to conduct a systematic review of Diosgenin, its pharmaceutical applications and perspectives on the application in the cardiovascular system diseases. METHODS: Periodic basis ScienceDirect, PubMed and Virtual Health Library were used as well as the European Office of the technological bases Patent Office, World Intellectual Property Organization, the Office US Patent and Trademark Office and the Brazilian database National Institute of Industrial Property. The keywords used were Diosgenin, cardiovascular system, hypertension, atherosclerosis and dyslipidemia and their correlations in English and Portuguese, with publications from January 2010 to June 2015. RESULTS: After analysis of the technological and scientific bases, the results show that there is a limited number of patents showing that the Diosgenin action on the cardiovascular system is centered in the areas for chemical studies of steroid Diosgenin. In the scientific basis, the results show that there is an interesting Diosgenin action on the cardiovascular system. CONCLUSION: Therefore, studies with Diosgenin are promising on account of its significant pharmacological potential from scientific and technological viewpoints, reaching for a technology transfer to generate economic and industrial growth.
