ABSTRACT
In this work a biofunctional composite coating architecture for controlled corrosion activity and enhanced cellular adhesion of AZ31 Mg alloys is proposed. The composite coating consists of a polycaprolactone (PCL) matrix modified with nanohydroxyapatite (HA) applied over a nanometric layer of polyetherimide (PEI). The protective properties of the coating were studied by electrochemical impedance spectroscopy (EIS), a non-disturbing technique, and the coating morphology was investigated by field emission scanning electron microscopy (FE-SEM). The results show that the composite coating protects the AZ31 substrate. The barrier properties of the coating can be optimized by changing the PCL concentration. The presence of nanohydroxyapatite particles influences the coating morphology and decreases the corrosion resistance. The biocompatibility was assessed by studying the response of osteoblastic cells on coated samples through resazurin assay, confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). The results show that the polycaprolactone to hydroxyapatite ratio affects the cell behavior and that the presence of hydroxyapatite induces high osteoblastic differentiation.
Subject(s)
Alloys/chemistry , Coated Materials, Biocompatible/chemistry , Durapatite/chemistry , Magnesium/chemistry , Materials Testing , Osteoblasts/metabolism , Polyesters/chemistry , Cell Line , Corrosion , Humans , Osteoblasts/cytologyABSTRACT
The high corrosion rate of magnesium alloys is the main drawback to their widespread use, especially in biomedical applications. There is a need for developing new coatings that provide simultaneously corrosion resistance and enhanced biocompatibility. In this work, a composite coating containing polyether imide, with several diethylene triamine and hydroxyapatite contents, was applied on AZ31 magnesium alloys pre-treated with hydrofluoric acid by dip coating. The coated samples were immersed in Hank's solution and the coating performance was studied by electrochemical impedance spectroscopy and scanning electron microscopy. In addition, the behavior of MG63 osteoblastic cells on coated samples was investigated. The results confirmed that the new coatings not only slow down the corrosion rate of AZ31 magnesium alloys in Hank's solution, but also enhance the adhesion and proliferation of MG63 osteoblastic cells, especially when hydroxyapatite nanoparticles were introduced in the coating formulation.
Subject(s)
Absorbable Implants , Alloys/pharmacology , Coated Materials, Biocompatible/pharmacology , Magnesium/pharmacology , Polymers/pharmacology , Cell Line , Cell Proliferation/drug effects , Cell Survival/drug effects , Corrosion , Dielectric Spectroscopy , Electrochemical Techniques , Humans , Osteoblasts/cytology , Osteoblasts/drug effects , Osteoblasts/ultrastructureABSTRACT
The present study investigates the pitting-corrosion behaviour of AISI 316L stainless steel in human physiological fluids. The emphasis is on the effect of diabetic serum with glucose and proteins, but reference solutions of isotonic saline solution with and without antibiotics were also used. Polarization experiments were carried out, and the results point to the innocuity of those fluids on the performance of the alloy as far as pitting corrosion is concerned.
