Assessment of hybrid fixed and moving bed biofilm applications for wastewater treatment capacity increase - In situ tests in El-Gouna WWTP, Egypt.

This paper provides a procedure for comparing the performance of different biofilm carrier medias and their surrounding suspended biomass through oxygen uptake rate (OUR) tests. For in situ (oxygen uptake rate (OUR) measurements, three identical lab scale biofilm reactors were set up at the El Gouna wastewater treatment plant (WWTP). In this setup, two options of media for moving-bed biofilm reactors (MBBR) and one media for fixed-bed biofilm reactors (FBBR) were compared. The WWTP also used the same carrier in a real scale hybrid application to analyze how the interactions between the carrier type and the suspended biomass influences the overall performance. The in situ OUR approach is recommended to measure the contribution of the biofilm fixed biomass under site specific conditions. Specifically, settleability and diffusion limitations are the two opposite poles that cannot be predicted adequately for mild climate conditions based on the literature. A biofilm carrier application can add but actually can also reduce the capacity in a hybrid activated sludge system: The added MBBR-media was able to grind down the sludge flocs forming a poorly settleable suspended biomass. The added FBBR-media can lead to extracellular polymeric substances (EPS) rich biofilms that contribute very little as substrate and oxygen are unavailable for the microorganisms present in the biofilm. In this application of the comparison procedure, Kaldnes K1 like MBBR media was compared with a recycling MBBR carrier option (poly propylene bottle caps) and Jäger Envirotech "BioCurlz™" FBBR media. The study showed higher average rates for the MBBR but decreased settleability. The FBBR showed higher peak rates when flushed to break up the biofilm and well settleable sludge. The determination of OUR per g of volatile solids (SOUR) showed comparable results for all the carriers and in warm conditions, only the capacity to accommodate biomass determines the contribution of the carrier.

Mathematical modelling and comparative analysis of treatment technologies for upgrading wastewater treatment plants: A case study of biofilm reactors in El-Gouna, Egypt.

In recent years, Moving Bed Biofilm Reactors (MBBRs) have been preferred to conventional processes with suspended biomass. The main reason for this preference is that it can achieve better removal efficiencies than conventional systems with smaller footprints. However, unlocking the full potential of MBBRs in large-scale WWTPs remains challenging in real life. In this study, the performance of three different treatment technologies, Extended Aeration Activated Sludge (EAAS), Hybrid Fixed Bed Biofilm Reactor (HFBBR), and Hybrid Moving Bed Biofilm Reactor (HMBBR), was investigated over a year in a WWTP located in El-Gouna, Egypt. The COD removal efficiencies of the three systems were comparable, with the EAAS achieving 93.5%, HFBBR 94%, and HMBRR 95%. Nevertheless, the NH4 removal efficiency of the EAAS was slightly lower (97.5%) than that of the HFBBR and the HMBBR, that achieved a removal efficiency of 98%. BioWin Software was able to mimic the real case of the WWTP of El-Gouna and critically defined all plant limitations and operational data. Different simulations were modeled to test the hydraulic and organic loading capacities of the three systems under different scenarios and operating conditions. The HMBBR system failed to withstand the increase in organic load because of the biomass sloughing effect and subsequently high TSS loads in the settlers. Biomass sloughing overloaded the settlers and lead to biomass loss in the effluent. As the settleability of the HMBBR sludge was significantly lower than for the HFBBR the TSS loss in the effluent happened that much earlier that the moving carrier application had an adverse effect contradicting with the primary purpose of adding media carriers. Model simulations and data analysis findings were used to recommend the most suitable configuration for upgrading an existing system using the attached growth technique with all kinetic parameters and operational conditions. The recommended configuration focuses mainly on the separation of plastic media in a compartment with a very low hydraulic retention time to absorb the incoming shock load.

Temperature effects on the aerobic metabolism of glycogen-accumulating organisms.

Short-term temperature effects on the aerobic metabolism of glycogen-accumulating organisms (GAO) were investigated within a temperature range from 10 to 40 degrees C. Candidatus Competibacter Phosphatis, known GAO, were the dominant microorganisms in the enriched culture comprising 93 +/- 1% of total bacterial population as indicated by fluorescence in situ hybridization (FISH) analysis. Between 10 and 30 degrees C, the aerobic stoichiometry of GAO was insensitive to temperature changes. Around 30 degrees C, the optimal temperature for most of the aerobic kinetic rates was found. At temperatures higher than 30 degrees C, a decrease on the aerobic stoichiometric yields combined with an increase on the aerobic maintenance requirements were observed. An optimal overall temperature for both anaerobic and aerobic metabolisms of GAO appears to be found around 30 degrees C. Furthermore, within a temperature range (10-30 degrees C) that covers the operating temperature range of most of domestic wastewater treatment systems, GAOs aerobic kinetic rates exhibited a medium degree of dependency on temperature (theta = 1.046-1.090) comparable to that of phosphorus accumulating organisms (PAO). We conclude that GAO do not have metabolic advantages over PAO concerning the effects of temperature on their aerobic metabolism, and competitive advantages are due to anaerobic processes.

Short-term temperature effects on the anaerobic metabolism of glycogen accumulating organisms.

Proliferation of glycogen accumulating organisms (GAO) has been identified as a potential cause of enhanced biological phosphorus removal (EBPR) failure in wastewater treatment plants (WWTP). GAO compete for substrate with polyphosphate accumulating organisms (PAO) that are the microorganisms responsible for the phosphorus removal process. In the present article, the effects of temperature on the anaerobic metabolism of GAO were studied in a broad temperature range (from 10 to 40 degrees C). Additionally, maximum acetate uptake rate of PAO, between 20 and 40 degrees C, was also evaluated. It was found that GAO had clear advantages over PAO for substrate uptake at temperatures higher than 20 degrees C. Below 20 degrees C, maximum acetate uptake rates of both microorganisms were similar. However, lower maintenance requirements at temperature lower than 30 degrees C give PAO metabolic advantages in the PAO-GAO competition. Consequently, PAO could be considered to be psychrophilic microorganisms while GAO appear to be mesophilic. These findings contribute to understand the observed stability of the EBPR process in WWTP operated under cold weather conditions. They may also explain the proliferation of GAO in WWTP and thus, EBPR instability, observed in hot climate regions or when treating warm industrial effluents. It is suggested to take into account the observed temperature dependencies of PAO and GAO in order to extend the applicability of current activated sludge models to a wider temperature range.