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1.
BMC Genomics ; 16: 524, 2015 Jul 14.
Article in English | MEDLINE | ID: mdl-26169280

ABSTRACT

BACKGROUND: The Xanthomonas citri pv. citri (X. citri) is a phytopathogenic bacterium that infects different species of citrus plants where it causes canker disease. The adaptation to different habitats is related to the ability of the cells to metabolize and to assimilate diverse compounds, including sulfur, an essential element for all organisms. In Escherichia coli, the necessary sulfur can be obtained by a set of proteins whose genes belong to the cys regulon. Although the cys regulon proteins and their importance have been described in many other bacteria, there are no data related to these proteins in X. citri or in the Xanthomonas genus. The study of the relevance of these systems in these phytopathogenic bacteria that have distinct mechanisms of infection is one essential step toward understanding their physiology. In this work, we used bioinformatics, molecular modeling and transcription analysis (RT-PCR) to identify and characterize the putative cys regulon genes in X. citri. RESULTS: We showed that the ATP Binding Cassette Transporter (ABC transporter) SbpCysUWA for sulfate uptake is conserved in X. citri and translated in presence of sulfate. On the other hand, differently from what is predicted in databases, according molecular modeling and phylogenetic analysis, X. citri does not show a proper taurine transporter, but two different ABC systems related to the alkanesulfonate/sulfonate transport that were recently acquired during evolution. RT-PCR analysis evidenced that these genes and their putative transcriptional regulator CysB are rather transcripted in XAM1, a medium with defined concentration of sulfate, than LB. CONCLUSIONS: The presence of at least three distinct systems for sulfate and sulfonates assimilation in X. citri evidenced the importance of these compounds for the bacterium. The transcription of genes involved with alkanesulfonate/sulfur compounds in XAM1 along to CysB suggests that despite the differences in the transporters, the regulation of these systems might be similar to the described for E. coli. Altogether, these results will serve as a foundation for further studies aimed to understanding the relevance of sulfur in growth, virulence and pathogenesis of X. citri and related bacteria.


Subject(s)
ATP-Binding Cassette Transporters/genetics , Phylogeny , Sulfur/metabolism , Xanthomonas/metabolism , ATP-Binding Cassette Transporters/metabolism , Alkanesulfonates/metabolism , Biological Transport/genetics , Citrus/microbiology , Escherichia coli/genetics , Plant Diseases/genetics , Plant Diseases/microbiology , Virulence/genetics , Xanthomonas/genetics
2.
Biochim Biophys Acta ; 1784(2): 393-9, 2008 Feb.
Article in English | MEDLINE | ID: mdl-18088604

ABSTRACT

In Xanthomonas axonopodis pv. citri (Xac or X. citri), the modA gene codes for a periplasmic protein (ModA) that is capable of binding molybdate and tungstate as part of the ABC-type transporter required for the uptake of micronutrients. In this study, we report the crystallographic structure of the Xac ModA protein with bound molybdate. The Xac ModA structure is similar to orthologs with known three-dimensional structures and consists of two nearly symmetrical domains separated by a hinge region where the oxyanion-binding site lies. Phylogenetic analysis of different ModA orthologs based on sequence alignments revealed three groups of molybdate-binding proteins: bacterial phytopathogens, enterobacteria and soil bacteria. Even though the ModA orthologs are segregated into different groups, the ligand-binding hydrogen bonds are mostly conserved, except for Archaeglobus fulgidus ModA. A detailed discussion of hydrophobic interactions in the active site is presented and two new residues, Ala38 and Ser151, are shown to be part of the ligand-binding pocket.


Subject(s)
Molybdenum/chemistry , Molybdenum/metabolism , Periplasmic Binding Proteins/chemistry , Periplasmic Binding Proteins/metabolism , Xanthomonas axonopodis/chemistry , Xanthomonas axonopodis/metabolism , Amino Acid Sequence , Binding Sites , Conserved Sequence , Crystallography, X-Ray , Ligands , Molecular Sequence Data , Periplasmic Binding Proteins/genetics , Phylogeny , Plant Diseases/microbiology , Protein Binding , Protein Structure, Tertiary , Sequence Alignment , Structural Homology, Protein , Xanthomonas axonopodis/genetics , Xanthomonas axonopodis/pathogenicity
3.
Protein Expr Purif ; 50(2): 215-22, 2006 Dec.
Article in English | MEDLINE | ID: mdl-16879982

ABSTRACT

The modABC operon of phytopathogen Xanthomonas axonopodis pv. citri (X. citri) encodes a putative ABC transporter involved in the uptake of the molybdate and tungstate anions. Sequence analyses showed high similarity values of ModA orthologs found in X. campestris pv. campestris (X. campestris) and Escherichia coli. The X. citri modA gene was cloned in pET28a and the recombinant protein, expressed in the E. coli BL21 (DE3) strain, purified by immobilized metal affinity chromatography. The purified protein remained soluble and specifically bound molybdate and tungstate with K(d) 0.29+/-0.12 microM and 0.58+/-0.14 microM, respectively. Additionally binding of molybdate drastically enhanced the thermal stability of the recombinant ModA as compared to the apoprotein. This is the first characterization of a ModA ortholog expressed by a phytopathogen and represents an important tool for functional, biochemical and structural analyses of molybdate transport in Xanthomonas species.


Subject(s)
ATP-Binding Cassette Transporters/isolation & purification , Bacterial Proteins/isolation & purification , Molybdenum/metabolism , Periplasmic Binding Proteins/isolation & purification , Xanthomonas/genetics , ATP-Binding Cassette Transporters/genetics , ATP-Binding Cassette Transporters/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Cloning, Molecular , Operon , Periplasmic Binding Proteins/genetics , Periplasmic Binding Proteins/metabolism , Protein Folding , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Thermodynamics , Tungsten Compounds/metabolism , Xanthomonas/metabolism
4.
Protein Expr Purif ; 43(2): 103-10, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16139753

ABSTRACT

The uptake of maltose and maltodextrins in gram-negative bacteria is mediated by an ATP-dependent transport complex composed of a periplasmic maltose-binding protein (MBP) and membrane-associated proteins responsible for the formation of a membrane pore and generation of energy to drive the translocation process. In this work, we report the purification and in vitro functional analysis of MBP, encoded by the malE gene, of the plant pathogen Xanthomonas citri, responsible for the canker disease affecting citrus plants throughout the world. The X. citri MBP is composed of 456 amino acids, displaying a low amino acid identity (16% throughout the sequence) compared to the Escherichia coli K12 ortholog. The X. citri malE gene was cloned into a pET28a vector, and the encoded protein was expressed and purified by affinity chromatography as a His-tag N-terminal fusion peptide produced by the E. coli BL21 strain. Enhanced levels of soluble protein were achieved with static cultures kept overnight at 23 degrees C. Ability to bind immobilized amylose, the emission of intrinsic fluorescence and circular dichroism spectra indicated that the purified recombinant protein preserved both conformation and biological activity of the native protein. The availability of the recombinant MBP will contribute to the functional and structural analysis of the maltose and maltodextrin uptake system of the plant pathogen X. citri.


Subject(s)
Bacterial Proteins/chemistry , Bacterial Proteins/isolation & purification , Carrier Proteins/chemistry , Carrier Proteins/isolation & purification , Plants/microbiology , Xanthomonas , Amino Acid Sequence , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Carrier Proteins/genetics , Carrier Proteins/metabolism , Escherichia coli K12/genetics , Escherichia coli K12/metabolism , Gene Expression , Maltose-Binding Proteins , Molecular Sequence Data , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , Xanthomonas/genetics , Xanthomonas/metabolism , Xanthomonas/pathogenicity
5.
Curr Microbiol ; 48(5): 354-9, 2004 May.
Article in English | MEDLINE | ID: mdl-15060731

ABSTRACT

The oligopeptide permease (Opp), a protein-dependent ABC transporter, has been found in the genome of Xanthomonas axonopodis pv. citri ( Xac), but not in Xanthomonas campestris pv. campestris ( Xcc). Sequence analysis indicated that 4 opp genes ( oppA, oppB, oppC, oppD/F), located in a 33.8-kbp DNA fragment present only in the Xac genome, are arranged in an operon-like structure and share highest sequence similarities with Streptomyces roseofulvus orthologs. Nonetheless, analyses of the GC content, codon usage, and transposon positioning suggested that the Xac opp operon does not have an exogenous origin. The presence of a stop codon at one of the ATP-binding domains of OppD/F would render the uptake system nonfunctional, but detection of a single polycistronic mRNA and periplasmic OppA in actively growing bacteria suggests that the Opp permease is active and could contribute to the distinct nutritional requirements and host specificities of the two Xanthomonas species.


Subject(s)
Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Membrane Transport Proteins/genetics , Membrane Transport Proteins/metabolism , Xanthomonas/enzymology , Xanthomonas/genetics , Bacterial Proteins/analysis , Bacterial Proteins/chemistry , Bacterial Proteins/isolation & purification , Base Composition , Blotting, Northern , Blotting, Western , Carrier Proteins/analysis , Carrier Proteins/genetics , Carrier Proteins/isolation & purification , Codon/analysis , Codon/genetics , Codon, Terminator , DNA Transposable Elements , Genes, Bacterial , Lipoproteins/analysis , Lipoproteins/genetics , Lipoproteins/isolation & purification , Membrane Transport Proteins/chemistry , Operon , Phylogeny , Protein Structure, Tertiary , RNA, Bacterial/analysis , RNA, Bacterial/isolation & purification , RNA, Messenger/analysis , RNA, Messenger/isolation & purification , Streptomyces/genetics , Xanthomonas/growth & development
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