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1.
Am J Hematol ; 52(4): 305-9, 1996 Aug.
Article in English | MEDLINE | ID: mdl-8701949

ABSTRACT

A 13-year-old African-American female with erythrocytosis and three different beta globins on electrophoresis beta A, beta S, and beta Osler, raised the possibility that one chromosome 11 might contain a duplicated beta globin gene, since there are normally only 2 beta globin genes. DNA sequence analysis showed GTG at codon 6 in exon 1, corresponding to Hb S and AAT at codon 145 in exon 3, indicating a substitution of Asn for Tyr. Thus, Hb Osler undergoes spontaneous post-translational deamidation, beta 145 Asn-->beta 145 Asp. Unmodified Hb Osler (Asn) co-migrates with Hb A on electrophoresis and co-elutes with Hb A on HPLC; therefore it has not been identified previously. All previous studies have incorrectly identified the mutation as being beta 145 (HC 2) Tyr-->Asp.


Subject(s)
DNA/chemistry , Globins/genetics , Hemoglobin, Sickle/genetics , Hemoglobins, Abnormal/genetics , Mutation , Adolescent , Base Sequence , Chromosomes, Human, Pair 11 , Exons , Female , Humans , Molecular Sequence Data , Sequence Analysis, DNA , Twins, Dizygotic
2.
Biochemistry ; 35(22): 7181-7, 1996 Jun 04.
Article in English | MEDLINE | ID: mdl-8679546

ABSTRACT

Hydrogen peroxide oxidation of human erythrocytes induces a transfer of phospholipid from the membrane into the cytosol [Brunauer, L.S., Moxness, M.S., & Huestis, W.H. (1994) Biochemistry 33, 4527-4532]. The current study examines the mechanism of lipid reorganization in oxidized cells. Exogenous phosphatidylserine was introduced into the inner monolayer of erythrocytes, and its distribution was monitored by microscopy and radioisotopic labeling. Pretreatment of cells with carbon monoxide prevented both hemoglobin oxidation and the transfer of phosphatidyserine into the cytosolic compartment. The roles of the various hemoglobin oxidation products in lipid extraction were investigated using selective oxidants. Nitrite treatment of intact cells produced almost complete conversion to methemoglobin, but no detectable lipid extraction. Treatments designed to produce the green hemoglobin derivatives, sulfhemoglobin and choleglobin, resulted in cytosolic extraction of phosphatidylserine. Ion exchange and size exclusion chromatography of oxidized cytosolic components revealed a lipid-hemoglobin complex. The interaction between lipid and hemoglobin oxidation products was verified in a model system. Purified hemoglobin, enriched in sulfhemoglobin and choleglobin by treatment with H2O2, H2S, or ascorbate, extracted phospholipid from small unilamellar phospholipid vesicles. Electron paramagnetic resonance studies demonstrated that hemoglobin oxidation products also adsorb fatty acids from solution. This newly described activity of hemoglobin may play a role in the clearance of oxidatively damaged and senescent cells from circulation.


Subject(s)
Erythrocyte Membrane/metabolism , Hemoglobins/metabolism , Membrane Lipids/metabolism , Phospholipids/metabolism , Ascorbic Acid/pharmacology , Carbon Monoxide/pharmacology , Cell Size/drug effects , Cyclic N-Oxides/metabolism , Cytoplasm/metabolism , Electron Spin Resonance Spectroscopy , Globins/metabolism , Humans , Hydrogen Peroxide/pharmacology , Hydrogen Sulfide/pharmacology , Metalloporphyrins/metabolism , Methemoglobin/metabolism , Nitrites/pharmacology , Oxidation-Reduction , Phosphatidylserines/metabolism , Sulfhemoglobin/metabolism
3.
Biochemistry ; 33(15): 4527-32, 1994 Apr 19.
Article in English | MEDLINE | ID: mdl-8161507

ABSTRACT

The effects of oxidative damage on membrane phospholipid organization were examined in human erythrocytes. Exposure to H2O2 induced shape changes in these cells; normal discocytes became echinocytic, and stomatocytes generated by foreign phosphatidylserine incorporation reverted to discoid morphology. H2O2 treatment also inhibited phosphatidylserine transport from the outer to inner membrane monolayer, consistent with earlier reports on oxidative sensitivity of the aminophospholipid translocator. The morphological changes are consistent with movement of inner monolayer lipids to the outer monolayer, as might be expected if aminophospholipid sequestration is compromised. However, lipid extraction and prothrombinase activation assays showed no increased exposure of phosphatidylserine on the cell surface. Instead, phosphatidylserine was found associated with the cytosolic fraction of H2O2-treated cells. These observations suggest that oxidative damage alters the lipid organization of erythrocyte membranes, not by randomizing the lipid classes within the bilayer, but by inducing extraction of inner monolayer components into the cytosol.


Subject(s)
Cytosol/metabolism , Erythrocyte Membrane/metabolism , Erythrocytes/drug effects , Hydrogen Peroxide/pharmacology , Phospholipids/blood , Adult , Calcium/pharmacology , Erythrocytes/cytology , Erythrocytes/metabolism , Humans , Hydrogen Peroxide/chemistry , Oxidation-Reduction , Phosphatidylserines/blood , Phosphatidylserines/pharmacology , Thromboplastin/metabolism , Vanadates/pharmacology
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