ABSTRACT
It is demonstrated by experiments with rabbits that the Ca2+-ATP-ase activity is stabilized when using combined anesthetics (diacetylcholine + halothane + N2O) as distinct from application of halothane. A decrease in the cholinesterase activity is less pronounced than under the halothane action but more than with the diacetylcholine application. A decrease in the Na+, K+-ATP-ase activity is observed with all types of anesthesia. A considerable inhibition of creatine kinase under the action of combined anesthesia and halothane and an increase of the lactate dehydrogenase activity under diacetylcholine application in mitochondria are shown. Reliable differences in the succinic dehydrogenase activity are not detected.
Subject(s)
Anesthetics/pharmacology , Myocardium/enzymology , Anesthesia, Inhalation , Anesthetics/administration & dosage , Animals , Calcium-Transporting ATPases/antagonists & inhibitors , Calcium-Transporting ATPases/metabolism , Cholinesterase Inhibitors , Cholinesterases/metabolism , Creatine Kinase/antagonists & inhibitors , Creatine Kinase/metabolism , Halothane/administration & dosage , Halothane/pharmacology , In Vitro Techniques , L-Lactate Dehydrogenase/antagonists & inhibitors , L-Lactate Dehydrogenase/metabolism , Mitochondria, Heart/enzymology , Nitrous Oxide/administration & dosage , Nitrous Oxide/pharmacology , Rabbits , Sarcolemma/enzymology , Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors , Sodium-Potassium-Exchanging ATPase/metabolism , Succinate Dehydrogenase/antagonists & inhibitors , Succinate Dehydrogenase/metabolism , Succinylcholine/administration & dosage , Succinylcholine/pharmacologyABSTRACT
The creatine kinase activity in the skeletal muscles and heart of rats is investigated under the effect of preparations of the depolarizing (myorelaxin) and nondepolarizing (tubocurarin, arduan) action. It is stated that arduan and tubocurarin inhibit the enzyme activity in the myocardium but not in skeletal muscles. The creatine kinase activity in the myocardium extract decreases twice under the tubocurarin effect and more than thrice under the arduan effect. Inhibition of the enzyme activity in the myocardium mitochondria under arduan effect is pronounced more greatly.
Subject(s)
Creatine Kinase/antagonists & inhibitors , Muscle Contraction/drug effects , Muscle Relaxation/drug effects , Muscles/enzymology , Myocardium/enzymology , Androstane-3,17-diol/analogs & derivatives , Androstane-3,17-diol/pharmacology , Animals , Pipecuronium , Piperazines/pharmacology , Rats , Succinylcholine/pharmacology , Tubocurarine/pharmacologyABSTRACT
Ca2+-ATPase of rats and rabbits skeletal muscle sarcolemma was studied as affected by relaxants: tubocurarine, myorelaxin and dioxonium, and by anesthetic fluothane in experiments in vitro and in vivo with lung artificial ventilation during curarization. The musclular relaxant, myorelaxin and anesthetic fluothane both in the experiments in vitro and in vivo inhibited the activity of the enzyme, dioxonium under these conditions caused no significant changes. Tubocurarin in vitro activated the enzyme and in vivo, vice versa, induced inhibition of the enzymic activity. The latter evidences for a many-sided character of the tubocurarine metabolic effect in the living organism. Combination of tubocurarine with fluothane had a less pronounced inhibitory effect on the activity of Ca2+-ATPase than the components used separately.
