[Isolation and various properties of leucine aminopeptidase from biological fluids of patients with multiple sclerosis]. / Vydelenie i nekotorye svoistva spetsificheskoi leitsinaminopeptidaziz biologicheskikh zhidkostei bol'nykh rasseiannym sklerozom.

Specific form of leucine aminopeptidase (which was distinct from other forms of the enzyme found in blood) was characterized by its physico-chemical properties--pH optimum, substrate specificity, electrophoretic mobility and molecular mass. The enzyme was isolated from biological fluids of patients with multiple sclerosis. Free and bound forms of the enzyme were detected in blood and cerebrospinal fluid. Estimation of the bound enzyme activity has a diagnostic significance.

[Phosphorus and calcium metabolism and its hormonal regulation in chronic kidney failure]. / Fosforno-kal'tsievyi obmen i ego gormonal'naia reguliatsiia pri khronicheskoi pochechnoi nedostatochnosti.

In order to elucidate the blood serum calcium, inorganic phosphorus, parathyroid hormone (PTH) and calcitonin (CT) content in the course of the development of chronic renal failure (CRF), 80 patients with chronile glomerulonephritis were examined. Of these, in 24 glomerular filtration (GF) was normal, whereas 56 had CRF of varying degree. Thirty-three out of the 80 patients had the nephrotic syndrome (NS). The parameters enumerated were also determined in 45 patients with CRF treated by hemodialysis. In patients without the NS, hypocalcemia was discovered only at the final predialysis stage of CRF, while in the presence of the NS, it was detectable in normal GF and was aggravated as CRF progressed. The PTH level ascended if GF was lower than 40 ml/min, not correlating with calcium concentration in the serum. A direct correlation was disclosed between the PTH level and that of inorganic phosphorus. The role of the latter in stimulation of PTH and CT secretion in CRF is discussed. In patients treated by hemodialysis, the PTH level significantly rose with treatment time increase. There was also a tendency toward elevation of CT concentration. The activity of the osseous fraction of blood serum alkaline phosphatase in patients treated by hemodialysis was increased in 61-84% of cases depending on the treatment period.

[Kinetic method for determination of isoenzymes of alkaline phosphatase in human blood serum]. / Kineticheskii metod opredeleniia izofermentov shchelochnoi fosfatazy syvorotki krovi cheloveka.

Resistance of liver, bone, intestinal and placental human alkaline phosphatase isozymes to the effect of L-phenylalanine and urea was studied. The combined treatment with urea and L-phenylalanine increased an accuracy of estimation due to inhibition of bone and intestinal isozymes. A special equation enabled to simplify the calculations. As shown by laboratory and clinical tests, the method developed is suitable for quantitative estimation of alkaline phosphatase isoenzymes.