Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Mol Cell Probes ; 24(3): 115-23, 2010 Jun.
Article in English | MEDLINE | ID: mdl-19948215

ABSTRACT

In the post-eradication era of wild polioviruses, the only remaining sources of poliovirus infection worldwide would be the vaccine-derived polioviruses (VDPVs). As the preponderance of countries certified to be polio-free has switched from OPV (oral poliovirus vaccine) to IPV (inactivated poliovirus vaccine), importation of recombinant evolved derivatives of vaccinal strains would have serious implication for public health. To test the robustness of the proposed RT-PCR screening analysis, eleven recombinant vaccine-derived polioviruses that were characterized previously by sequencing by our group, in addition to three recently identified recombinant environmental isolates were assayed. Although the most definitive characterization of VDPVs is by genomic sequencing, in this study we describe a new, inexpensive and broadly applicable RT-PCR assay for the identification of the predominant recombination types S3/Sx in 2C and S2/Sx in 3D genomic regions respectively of VDPVs, that can be readily implemented in laboratories lacking sequencing facilities as a first approach for the early detection of vaccine-derived poliovirus (VDPVs).


Subject(s)
Genome, Viral/genetics , Poliovirus/genetics , RNA, Viral/genetics , Reverse Transcriptase Polymerase Chain Reaction/methods , DNA Primers/genetics , Humans , Molecular Sequence Data , Poliomyelitis/immunology , Poliomyelitis/virology , Poliovirus/immunology , Poliovirus/isolation & purification , Poliovirus Vaccine, Inactivated/immunology , Poliovirus Vaccine, Oral/immunology , Recombination, Genetic , Reproducibility of Results , Sequence Analysis, DNA , Sewage/virology , Virus Shedding
SELECTION OF CITATIONS
SEARCH DETAIL
...