Purification of the monoclonal heparin antibody H-1.18.

An antibody of the (immunoglobulin) IgG1 subclass against heparin was purified. Here we report on the purification of the heparin antibody. Ammonium sulfate precipitation was performed and showed a high purity of the precipitate. In the heparin radioimmunoassay it showed a high heparin binding. Capillary electrophoresis showed that albumin and other proteins were separated from the heparin antibody. The purification method allowed a large scale production of the heparin antibody.