ABSTRACT
Bacterial biomass may serve as an important environmental cleaning agent to toxic heavy metal ions at the expense of chemical processes which are not environmentally friendly. This study aimed at characterizing bacterial agents which could serve as a potential in situ bioremediation agent at the site of isolation. The characterization was performed using both phenotypic and molecular approaches. A novel Pseudomonas aeruginosa strain Pseudomonas aeruginosa Zambia SZK17 Kabwe1 was successfully isolated, identified, and characterized. The strain showed a promising tolerance to heavy metals such as copper (2 mM), zinc, nickel (2 mM), cobalt (1 mM), and cadmium (0.5 mM) at the laboratory level. The bacterium has shown the bioaccumulation of at least 60% of copper (II) sulfate (0.3655 mg/l) with R = 69.75%, cadmium (II) chloride (0.0241 mg/l) with R = 69.98%, zinc (II) chloride (0.1389 mg/l) with R = 69.91%, nickel (II) chloride (0.1155 mg/l) with R = 69.92%, and cobalt (II) chloride (0.593 mg/l) with R = 69.92%. The highest bioaccumulation has been observed in heavy metals cadmium, zinc, nickel, and cobalt. Characterization of the bacterium on pH has revealed that at a very high pH (≥ 9) and lower (≤ 5.5) pH, the bacterium tended to have reduced growth with optimum growth at pH 8. The high temperature at around 40 °C had a negative effect on the growth performance of the bacterium while optimum growth was observed at 28 °C. This novel P. aeruginosa strain has shown the phenotypic attributes to become a potential bioremediation agent; however, further investigation needs to be done to understand the genes and or molecular mechanisms that drive their tolerance to multiple heavy metals.