LINC00346 accelerates the malignant progression of colorectal cancer via competitively binding to miRNA-101-5p/MMP9.

The article "LINC00346 accelerates the malignant progression of colorectal cancer via competitively binding to miRNA-101-5p/MMP9, by W.-H. Tong, J.-F. Mu, S.-P. Zhang, published in Eur Rev Med Pharmacol Sci 2020; 24 (12): 6639-6646-DOI: 10.26355/eurrev_202006_21650-PMID: 32633353" has been withdrawn from the authors since they decided to perform further experiments. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/21650.

[Time-series analysis on the relationship between ambient PM2.5 and daily outpatient visits due to allergic conjunctivitis among children in Shenzhen].

Objective: To explore the short-term effects of ambient PM2.5 on the outpatient visits of allergic conjunctivitis among children in Shenzhen. Methods: It was a ecological study. Data on daily visits including date of visit, sex and age from children with allergic conjunctivitis were collected from Shenzhen Eye Hospital and Shenzhen Children's Hospital in 2018. Related data on air pollution (PM2.5, PM10, SO2, NO2, CO and O3) and meteorology (atmospheric pressure, temperature and relative humidity) were also collected. Pearson correlation analysis was used for normal distribution data and Spearman rank correlation analysis was used for non-normal distribution data. Generalized additive model was used to estimate the impact of PM2.5 pollution on allergic conjunctivitis outpatients and the lagging effects. Results: In 2018, there were 16 133 allergic conjunctivitis outpatients in the two hospitals. The maximum age was 18 years and the minimum age was 2 months. Males accounted for 49.3%. The daily average concentration of PM2.5 was 22 (15, 31) µg/m3. Changes of the concentration of PM2.5 had a positive correlation with the amount of allergic conjunctivitis visits, and the Spearman correlation coefficient was 0.150 (P=0.004). The single pollutant model showed that the strongest effect appeared at 3 days (RR=1.111， 95%CI:1.071-1.152). A 10 µg/m3 increase of PM2.5 would result in an excessive number of allergic conjunctivitis outpatients as much as 11.112% (95%CI：7.011%-15.212%). In the multiple air pollutants models, after the introduction of NO2, O3 and CO, the concentration of PM2.5 showed an enhanced effect on the number of hospital visits due to allergic conjunctivitis on the same day, and the difference was statistically significant (P<0.05). Conclusion: Changes of the concentration of PM2.5 had a positive correlation with daily outpatient visits of allergic conjunctivitis among children in Shenzhen. (Chin J Ophthalmol, 2020, 56: 608-614).

LINC00346 accelerates the malignant progression of colorectal cancer via competitively binding to miRNA-101-5p/MMP9.

OBJECTIVE: To clarify the promotive effect of LINC00346 on the malignant progression of colorectal cancer (CRC) by mediating miRNA-101-5p/MMP9 axis. PATIENTS AND METHODS: Expression pattern of LINC00346 in 46 paired CRC tissues and adjacent normal tissues was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Correlation between LINC00346 level and prognosis of CRC patients was analyzed, and the LINC00346 level in CRC cell lines was examined as well. Subsequently, potential influences of LINC00346 on cellular behaviors of CRC cells were evaluated through cell counting kit-8 (CCK-8), colony formation, transwell, and wound healing assays. Finally, Dual-Luciferase reporter gene assay was conducted to verify the binding relationship between LINC00346 and miRNA-101-5p/MMP9. RESULTS: LINC00346 was upregulated in CRC tissues and cell lines. Compared with CRC patients with low level of LINC00346, those with high level suffered a poorer prognosis, and higher metastatic rates (lymph node metastasis and distant metastasis). Transfection of sh-LINC00346 attenuated proliferative, migratory, and invasive abilities of CRC cells. In addition, LINC00346 was confirmed to bind to miRNA-101-5p, and the latter was binding to MMP9. Moreover, the overexpression of miRNA-101-5p decreased colony number, viability, and numbers of migratory and invasive cells. CONCLUSIONS: LINC00346 is upregulated in CRC and correlated with metastasis and poor prognosis of CRC. LINC00346 accelerates the malignant progression of CRC via targeting miRNA-101-5p/MMP9.

Expression of miR-31 in rectal cancer patients and its effect on proliferation ability of rectal cancer cells SW837.

OBJECTIVE: This study aimed to investigate the expression of miR-31 in rectal cancer patients and its effect on the proliferation and invasion ability of human rectal cancer cells SW837. PATIENTS AND METHODS: 55 rectal cancer cancerous tissue specimens and 55 corresponding adjacent tissue (tissue adjacent to carcinoma) specimens were collected from rectal cancer patients treated in The First Hospital of Jilin University from March 2014 to March 2015. Real Time-quantitative Polymerase Chain Reaction was used for detecting the expression level of miR-31 in cancerous tissue and corresponding adjacent tissues. Differences in the expression of miR-31 were compared between the two groups. Different miR-31 expression vectors were established and rectal cancer cells SW837 were transfected. MTT was used for detecting the proliferation ability of the cells in the miR-31-mimics group, miR-31-inhibitor group and miR-control group. RESULTS: The expression level of miR-31 was significantly higher in rectal cancer tissues than that in the adjacent tissues (p<0.05). The expression of miR-31 was higher in the miR-31-mimics group (23.6±4.6) than that in the miR-control group (1.63±0.65), while the expression of miR-31 was lower in the miR-31-inhibitor group (0.65±0.23) than that in the miR-control group. The proliferation ability of cells at the 6th, 12th, 24th, 48th, and 72nd hours was higher in the miR-31-mimics group than in the miR-31-inhibitor group, while that of cells was significantly lower in the miR-31-inhibitor group than in the miR-control group, with statistically significant differences (p<0.05). The number of invasive membrane cells (cell membrane number) counted under a microscope was (84.2±10.6) cells in the miR-31-mimics group, (12.3±4.1) cells in the miR-31-inhibitor group, and (45.2±10.6) cells in the miR-control group. The invasion ability in vitro of SW837 cells significantly increased after the overexpression of miR-31 (p<0.05). CONCLUSIONS: miR-31 is increasingly expressed in rectal cancer. Low expression of miR31 can inhibit the proliferation and invasion ability of the cells. MiR-31 is expected to become a current biotherapeutic target.

Monocytes-macrophages phagocytosis as a potential marker for disease resistance in generation 1 of dwarf chickens.

Monocytes-macrophages play an indispensable role in the immune system. The current study investigated the effect of selection for monocytes-macrophages phagocytosis on disease resistance in generation 1 (G1) of dwarf chickens. Five hundred dwarf chickens of generation 0 (G0) were divided into high and low phagocytic index (PI) groups (HPIG and LPIG, respectively) based on their PI of monocytes-macrophages at 290 d of age. Then, 2 x 2 mating combinations were conducted. Sixty G0 chickens from another dwarf chicken group were used to measure the levels of monocytes-macrophages phagocytosis at different developmental stages. Among a total of 2,500 randomly selected G1 chickens, 2,100 individuals were used for a surviving and growing test under adverse feeding circumstances, and the other 400 individuals were tested for Salmonella Pullorum challenge. The results showed that progenies of HPIG hens (female symbol) were more resistant to Salmonella Pullorum. After challenge, the death rate of progeny from HPIG female symbol (28.9%) was only 58% that of progeny from LPIG female symbol (49.4%, P < 0.001). In addition, the natural infection rate of Salmonella Pullorum before 207 d for offspring from HPIG female symbol (35.0%) was significantly lower than that for offspring from LPIG female symbol (48.3%, P < 0.001). The natural mortality before 56 d in progeny of HPIG female symbol (22.6%) was significantly lower than that in progeny of LPIG female symbol (29.1%) with a P-value of 0.001. The G1 chickens of HPIG G0 female symbol weighed more than those born to LPIG G0 female symbol at 28 and 42 d of age, whereas the difference was not statistically significant at 56 d of age. The heritability of monocytes-macrophages phagocytosis was 0.40, which was moderate. The PI values were at a low level before 126 d and increased dramatically until they declined significantly after 294 d. It could be concluded that phagocytosis of monocytes-macrophages is a marker for breeding excellent progeny with strong disease resistance.

Purification, characterization and sequence determination of a double-headed trypsin inhibitor peptide from Trichosanthes kirilowii (a Chinese medical herb).

A double-headed trypsin inhibitor peptide was isolated and purified from the root of Trichosanthes kirilowii Maxim (Cucurbitaceae), a Chinese medical herb, by 2.5% trichloroacetic acid and heat treatment followed by affinity chromatography with immobilized trypsin and ion-exchange chromatography. This inhibitor, consisting of 41 amino-acid residues with three pairs of disulfide bonds was sequenced. Two active domains were found to be located at two disulfide loops composed of eight (Pos. 17-24) and nine (Pos. 29-37) amino-acid residues, respectively. It inhibits two molecules of trypsin simultaneously and might be regarded as the smallest double-headed trypsin inhibitor (Mr = 4575) so far known. The chemical modification of the inhibitor with cyclohexandione and citraconic anhydride showed that Arg20-Gly21 and Lys30-Leu31 corresponded to the two reactive sites, respectively. The discovery of the Trichosanthes inhibitor is of importance not only for the study on the structure-function relationship of proteinase inhibitor peptides but also for the search for low molecular mass inhibitors of clinical value among Chinese medical herbs.