ABSTRACT
Diosgenin can inhibit the proliferation and cause apoptosis of various tumor cells, and its inhibitory effect on oral squamous cell carcinoma (OSCC) and its mechanism are still unclear. In this study, we predicted the targets of diosgenin for the treatment of OSCC through the database, then performed bioinformatics analysis of the targets, and further verified the effect of diosgenin on the activity of OSCC cell line HSC-3, the transcriptional profile of the targets and the molecular docking of the targets with diosgenin. The results revealed that there were 146 potential targets of diosgenin for OSCC treatment, which involved signaling pathways such as Ras, TNF, PI3K-AKT, HIF, NF-κB, and could regulate cellular activity through apoptosis, autophagy, proliferation and differentiation, inflammatory response, DNA repair, etc. Diosgenin significantly inhibited HSC-3 cell activity. The genes such as AKT1, MET1, SRC1, APP1, CCND1, MYC, PTGS2, AR, NFKB1, BIRC2, MDM2, BCL2L1, MMP2, may be important targets of its action, not only their expression was regulated by diosgenin but also their proteins had a high binding energy with diosgenin. These results suggest that diosgenin may have a therapeutic effect on OSCC through AKT1, MMP2 and other targets and multiple signaling pathways, which is of potential clinical value.
Subject(s)
Carcinoma, Squamous Cell , Diosgenin , Head and Neck Neoplasms , Mouth Neoplasms , Humans , Carcinoma, Squamous Cell/drug therapy , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/metabolism , Squamous Cell Carcinoma of Head and Neck , Matrix Metalloproteinase 2/pharmacology , Diosgenin/pharmacology , Phosphatidylinositol 3-Kinases/metabolism , Molecular Docking Simulation , Mouth Neoplasms/drug therapy , Mouth Neoplasms/genetics , Mouth Neoplasms/metabolism , Apoptosis , Cell Line, Tumor , Cell Proliferation , Proto-Oncogene Proteins c-akt/metabolismABSTRACT
Su wen shi yi (Annotated Plain Questions), compiled by Zhang Qi in the 9th year of Daoguang reign, was an important revised edition of Su wen (Plain Questions) which has over 570 entries of revised texts. Among them, about a half was inferred proofreadings which is a unique point of this book. This is closely related to the prevalence of scholastic atmosphere of textual research at that time. During his inferred proofreading, Zhang checked the original texts of Plain Questions and Wang Bing's texts as well. The exact method for his inferred proofreading included checking based on the original texts, checking based on medical doctrines, and combined method of both. The inferred proofreading method is of high reference value, exerting certain influence on the studies of Nei jing (Inner Canon) both domestically and abroad.
