Hemodynamic profiles of arterial hypertension with ambulatory blood pressure monitoring.

Blood pressure (BP) measurements obtained during a twenty-four-hour ambulatory blood pressure monitoring (24 h ABPM) have not been reliably applied to extract arterial hemodynamics. We aimed to describe the hemodynamic profiles of different hypertension (HT) subtypes derived from a new method for total arterial compliance (Ct) estimation in a large group of individuals undergoing 24 h ABPM. A cross-sectional study was conducted, which included patients with suspected HT. Cardiac output, Ct, and total peripheral resistance (TPR) were derived through a two-element Windkessel model without having a pressure waveform. Arterial hemodynamics were analyzed according to HT subtypes in 7434 individuals (5523 untreated HT and 1950 normotensive controls [N]). The individuals mean age was 46.2 ± 13.0 years; 54.8% were male, and 22.1% were obese. In isolated diastolic hypertension (IDH), the cardiac index (CI) was greater than that in normotensive (N) controls (CI: IDH vs. N mean difference 0.10 L/m/m2; CI 95% 0.08 to 0.12; p value <0.001), with no significant clinical difference in Ct. Isolated systolic hypertension (ISH) and divergent systolic-diastolic hypertension (D-SDH) had lower Ct values than nondivergent HT subtype (Ct: divergent vs. nondivergent mean difference -0.20 mL/mmHg; CI 95% -0.21 to -0.19 mL/mmHg; p value <0.001). Additionally, D-SDH displayed the highest TPR (TPR: D-SDH vs. N mean difference 169.8 dyn*s/cm-5; CI 95% 149.3 to 190.3 dyn*s/cm-5; p value <0.001). A new method is provided for the simultaneous assessment of arterial hemodynamics with 24 h ABPM as a single diagnostic tool, which allows a comprehensive assessment of arterial function for hypertension subtypes. Main hemodynamic findings in arterial HT subtypes with regard to Ct and TPR. The 24 h ABPM profile reflects the state of Ct and TPR. Younger individuals with IDH present with a normal Ct and frequently increased CO. Patients with ND-SDH maintain an adequate Ct with a higher TPR, while subjects with D-SDH present with a reduced Ct, high PP and high TPR. Finally, the ISH subtype occurs in older individuals with significantly reduced Ct, high PP and a variable TPR proportional to the degree of arterial stiffness and MAP values. There was an observed increase in PP with age in relation to the changes in Ct (see also text). SBP: systolic blood pressure; DBP: diastolic blood pressure; MAP: mean arterial pressure; PP: pulse pressure; N: normotension; HT: hypertension; IDH: isolated diastolic hypertension; ND-SDH: nondivergent systole-diastolic hypertension; D-SDH: divergent systolic-diastolic hypertension; ISH: isolated systolic hypertension; Ct: total arterial compliance; TPR: total peripheral resistance; CO: cardiac output; 24 h ABPM: 24 h ambulatory blood pressure monitoring.

Qualitative Analysis of a Teacher's Reflections on Medical Students Using Public Narratives to Promote Health.

A qualitative, inductive content analysis was done to explore a teacher's reflections on the experiences of a team of medical students using public narratives to promote health within a Colombian university. Data were collected from the teacher's written reflective journal and an academic report, the latter, submitted by the medical students. 'Being mobilized' emerged as an overarching category. The experiences were described in three interrelated categories: team involvement, barriers and countering these barriers. In terms of team involvement, students were motivated, committed, connected with people and frustrated. On the other hand, barriers such as doubts and discomfort remarked, and finally these barriers were countered through training, practice and teamwork. As conclusion, the public narrative framework has changed the way medical students think about how to mobilize people for health promotion in a university. Narrative approaches are gaining strength as sources of motivation to support the adoption of healthy behaviors.

Genetic engineering of microalgae for enhanced lipid production.

Microalgae have the potential to become microbial cell factories for lipid production. Their ability to convert sunlight and CO2 into valuable lipid compounds has attracted interest from cosmetic, biofuel, food and feed industries. In order to make microalgae-derived products cost-effective and commercially competitive, enhanced growth rates and lipid productivities are needed, which require optimization of cultivation systems and strain improvement. Advances in genetic tool development and omics technologies have increased our understanding of lipid metabolism, which has opened up possibilities for targeted metabolic engineering. In this review we provide a comprehensive overview on the developments made to genetically engineer microalgal strains over the last 30 years. We focus on the strategies that lead to an increased lipid content and altered fatty acid profile. These include the genetic engineering of the fatty acid synthesis pathway, Kennedy pathway, polyunsaturated fatty acid and triacylglycerol metabolisms and fatty acid catabolism. Moreover, genetic engineering of specific transcription factors, NADPH generation and central carbon metabolism, which lead to increase of lipid accumulation are also reviewed.

Enseñanza y aprendizaje de los determinantes sociales en salud en la región de las Américas / Teaching and learning of social determinants in health in the region of the Americas

Introducción: La región de las Américas es considerada como la más desigual del mundo. Los recursos humanos en salud resultan fundamentales para superar estas diferencias mediante el abordaje de los Determinantes Sociales. Objetivo: Revisar los procesos de enseñanza y aprendizaje de los Determinantes Sociales en programas de ciencias de la salud en la región de las Américas. Métodos: Se realizó una revisión de la literatura mediante la búsqueda de documentos oficiales y artículos en las bases de datos Web of Science, Clinicalkey, PubMed, Science Direct y Lilacs. Se seleccionaron 68 documentos para su análisis. Resultados: La región de las Américas ha tenido un avance significativo en cuanto a la inclusión de las políticas públicas que actúan sobre los Determinantes y que ayudan a que los recursos humanos en salud se formen con enfoque de atención primaria. Sin embargo, es importante que las facultades de ciencias de la salud de la región profundicen en el conocimiento de estrategias pedagógicas y de investigación, y que faciliten la enseñanza y el aprendizaje de los Determinantes. A su vez, se observan avances en la inclusión de la Educación Interprofesional en la región. Conclusiones: Los programas de ciencias de la salud deben profundizar en la aplicación de estrategias pedagógicas y de investigación, que faciliten la enseñanza y el aprendizaje de los Determinantes, los cuales promueven la reflexión del papel de profesional en la disminución de las inequidades sociales y el mejoramiento en la salud de la población(AU)

Introduction: The region of the Americas is considered as the most unequal in the world. Human resources in health are essential for overcoming these differences, by means of addressing social determinants. Objective: To review the processes of teaching and learning about social determinants in health science programs in the region of the Americas. Methods: A literature review was carried out by searching for official documents and articles in the databases ofWeb of Science, Clinicalkey, PubMed, Science Direct and Lilacs databases. Sixty-eight documents were selected for the analysis. Results: The region of the Americas has made significant progress in terms of including public policies influencing on determinants and helping human resources in health to be trained under a primary care approach. However, it is important thathealth sciences schools in the region deepen their knowledge of pedagogical and research strategies, as well as they facilitate teaching and learning of the determinants. At the same time, progress has been observed regarding the inclusion of interprofessional education in the region. Conclusions: Health sciences programs should deepen the application of pedagogical and research strategies that facilitate teaching and learning of the determinants, which foster reflection about the role of professionals in reducing social inequalities and improving population health(AU)

New obstetric systemic inflammatory response syndrome criteria for early identification of high-risk of sepsis in obstetric patients.

OBJECTIVES: We aimed to establish new cut-off values for SIRS (Systemic Inflammatory Response Syndrome) variables in the obstetric population. METHODS: A prospective cohort study in pregnant and postpartum women admitted with systemic infections between December 2017 and January 2019. Patients were divided into three cohorts: Group A, patients with infection but without severe maternal outcomes (SMO); Group B, patients with infection and SMO or admission to the intensive care unit (ICU); and Group C, a control group. Outcome measures were ICU admission and SMO. The relationship between SIRS criteria and SMO was expressed as the area under the receiver operating characteristics curve (AUROC), selecting the best cut-off for each SIRS criterion. RESULTS: A total of 541 obstetric patients were enrolled, including 341 with infections and 200 enrolled as the reference group (Group C). The patients with infections included 313 (91.7%) in Group A and 28 (8.2%) in Group B. There were significant differences for all SIRS variables in Group B, compared with Groups A and C, but there were no significant differences between Groups A and C. The best cut-off values were the following: temperature 38.2 °C, OR 4.1 (1.8-9.0); heart rate 120 bpm, OR 2.9 (1.2-7.4); respiratory rate 22 bpm, OR 4.1 (1.6-10.1); and leukocyte count 16,100 per mcl, OR 3.5 (1.6-7.6). CONCLUSIONS: The cut-off values for SIRS variables did not differ between healthy and infected obstetric patients. However, a higher cut-off may help predict the population with a higher risk of severe maternal outcomes.

A novel method for the noninvasive estimation of cardiac output with brachial oscillometric blood pressure measurements through an assessment of arterial compliance.

OBJECTIVE: To propose and validate a new method for estimating cardiac output based on the total arterial compliance (Ct) formula that does not need an arterial waveform and to apply it to brachial oscillometric blood pressure measurements (OBPMs). METHODS: One hundred subjects with normal heart anatomy and function were included. Reference values for cardiac output were measured with echocardiography, and Ct was calculated with a two-element Windkessel model. Then, a statistical model of arterial compliance (Ce) was used to estimate cardiac output. Finally, the measured and estimated cardiac output values were compared for accuracy and reproducibility. RESULTS: The model was derived from the data of 70 subjects and prospectively tested with the data from the remaining 30 individuals. The mean age of the whole group was 43.4 ± 12.8 years, with 46% women. The average blood pressure (BP) was 107.1/65.0 ± 15.0/9.6 mmHg and the average heart rate was 67.7 ± 11.4 beats/min. The average Ct was 1.39 ± 0.27 mL/mmHg and the average cardiac output was 5.5 ± 1.0 L/min. The mean difference in the cardiac output estimated by the proposed methodology vs. that measured by Doppler echocardiography was 0.022 L/min with an SD of 0.626 L/min. The intraclass correlation coefficient was 0.93, and the percentage error was 19%. CONCLUSION: Cardiac output could be reliably and noninvasively obtained with brachial OBPMs through a novel method for estimating Ct without the need for an arterial waveform. The new method could identify hemodynamic factors that explain BP values in an ambulatory care setting.

Theatre Connect: Key Strategies for Facilitating LGBTQQ Youth Theatre Programs.

Youth who identify as lesbian, gay, bisexual, transgender, queer, or questioning (LGBTQQ) often experience heterosexism, homophobia, prejudice, and bullying in addition to the typical demands of adolescent development. Applied theatre programs have been shown to empower youth, improve mental health and well-being, and create positive identity and interpersonal relationships and, as such, have the potential to strengthen a range of protective factors for LGBTQQ youth. However, when programs engage participants in personal narratives, practitioners must be prepared to deftly navigate between the two domains of theatre in health and drama therapy. Since 2017, the University of Florida's (UF) Center for Arts in Medicine has offered an afterschool theatre program for LGBTQQ youth in partnership with clinicians from UF Health's Youth Gender Clinic and faculty in the Mental Health Counseling training program in the College of Education. Theatre practitioners lead the program in partnership with mental health professionals, who participate in sessions and are "on call" for participants. Program facilitators have developed a set of guidelines for organizations attempting to start LGBTQQ or other youth theatre programs in their local communities, which include the following recommendations: (1) prioritize safe and ethical practice through creating sustainable partnerships between mental health counsellors, experienced theatre practitioners, and local LGBTQQ organizations; (2) develop a clear contract between participants and facilitators regarding program goals; (3) utilize Baim's drama spiral as a conceptual tool and limit program activities to the first four spiral rings; (4) emphasize "play" and skill building rather than LGBTQQ topics.

Susceptibility to Insecticides and Natural Infection in Aedes aegypti: An Initiative to Improve the Mosquito Control Actions in Boyacá, Colombia.

Background: Integrated management strategies for dengue prevention and control have been the main way to decrease the transmission of arboviruses transmitted by A. aegypti in Colombia. However, the increase of chikungunya (CHIKV), Zika, and dengue (DENV) fever cases suggests deficiencies in vector control strategies in some regions from this country. Objective: This work aimed to establish a baseline susceptibility profile of A. aegypti to insecticides, determine the presence of kdr mutations associated with resistance to pyrethroids, and detect natural arbovirus infection in this vector from Moniquirá - Boyacá, one of the most endemic cities in Colombia. Methods: Mosquitos were collected in six neighborhoods, and colonies established in the laboratory. Susceptibility to malathion and lambda-cyhalothrin insecticides was evaluated, and we examined the point mutations present in portions of domains I, II, III, and IV of the sodium channel gene using a simple allele-specific PCR-based assay (AS-PCR). Findings: A. aegypti from Moniquirá showed decreased susceptibility to pyrethroid insecticides, and kdr mutations 419L, 1016I, and 1558C with allelic frequencies of 0.39, 0.40 and 0.95, respectively, were observed. The minimal infection rate (MIR) to DENV-1 was 44.1, while to CHIKV was 14.7. Conclusions: We establish a baseline insecticide resistance, kdr mutations, and arbovirus circulation, which contain the elements necessary for the consolidation of a local surveillance strategy with an early warning system and rational selection and rotation of insecticides.

Effect of Single and Combined Expression of Lysophosphatidic Acid Acyltransferase, Glycerol-3-Phosphate Acyltransferase, and Diacylglycerol Acyltransferase on Lipid Accumulation and Composition in Neochloris oleoabundans.

Microalgal lipids are promising feedstocks for food and biofuels. Since lipid production by microalgae is not yet economically feasible, genetic engineering is becoming a promising strategy to achieve higher lipid accumulation and productivities. Enzymes involved in the Kennedy pathway such as glycerol-3-phosphate acyltransferase (GPAT), lysophosphatidic acid acyltransferase (LPAT), and diacylglycerol acyltransferase (DGAT) catalyze key steps in the formation of triacylglycerol, which is the main constituent of lipids in N. oleoabundans. The overexpression of these enzymes in the targeted strain has a great potential to further increase their triacylglycerol content. We overexpressed single and multiple encoding genes for LPAT, GPAT, and DGAT from Acutodesmus obliquus in N. oleoabundans. Strains overexpressing single genes produced up to 52% and 45% g · gDW-1, which corresponds to 1.3- and 1.4-fold increase in total fatty acids and triacylglycerols, respectively. The orchestrated expression of the three genes resulted in 49% and 39% g · gDW-1, which is 1.2-folds increase in total fatty acids and triacylglycerols. Single expression of LPAT, GPAT, and DGAT genes resulted in higher lipid productivities during starvation without a significant effect on growth and photosynthetic activity during replete conditions. On the other hand, the simultaneous expression of LPAT, GPAT, and DGAT genes resulted in 52% lower growth rate, 14% lower photosynthetic activity and 4-folds increase in cell diameter. Moreover, the multigene expressing line showed a decrease in carbohydrates and protein content and an increase in pigments during nitrogen starved condition. The single and multiple expression of heterologous genes LPAT, GPAT, and DGAT showed to significantly enhanced the lipid accumulation in N. oleoabundans. Single gene expression resulted in higher lipid production and productivities without having a significant impact in the physiological status of the strains. This approach shows the potential for the generation of microalgal strains with higher economical potential for the production of lipids.

An educational strategy that promotes healthy habits in elderly people with hypertension in a municipality of Colombia: a participatory action research study. / Estrategia educativa que promueve los hábitos saludables en adultos mayores con hipertensión arterial en un municipio de Colombia: estudio de investigación-acción participativa.

OBJECTIVE: To identify resources of the municipality of Sopó-Cundinamarca, Colombia, that are also opportunities to strengthen the development of an educational strategy that promotes healthy habits (healthy diet and exercise) as part of the comprehensive management of hypertension in the elderly. METHODS: A qualitative study of a participatory-action research initiative in the Community Day Center of Sopó in the second semester of 2015. It was developed in three stages: first, a community diagnosis showed the need to integrate the culture, traditions and resources of the municipality as inputs that allow the adherence of healthy styles by the elderly for the control of hypertension; in the second stage, a work plan was established based on actions provided by the community; and in the third stage, we reflected on the results. RESULTS: An effective and sustainable intervention for the elderly can be achieved through the following activities: appropriation of the agricultural resources, the strengthening of dance as a form of exercise, use of motivational strategies, support of institutions that work with the welfare of the elderly, and the empowerment of facilitators. CONCLUSIONS: Interventions aimed at supporting the adherence of healthy lifestyles to the elderly should include and preserve the context of the community of which they are part, where community resources are the inputs that allow health promotion.

OBJETIVO: Identificar los recursos del municipio de Sopó-Cundinamarca, Colombia, como oportunidades de fortalecimiento para el desarrollo de una estrategia educativa que promueva los hábitos saludables (alimentación y actividad física) como parte del manejo integral de la hipertensión arterial en el adulto mayor. MÉTODOS: Estudio cualitativo, tipo investigación-acción participativa en la comunidad del centro día de Sopó en el segundo semestre de 2015. Se desarrolló en tres etapas. En la primera, a partir del contacto con la comunidad, se obtuvo un diagnóstico comunitario. Este fue la necesidad de integrar la cultura, las tradiciones y los recursos del municipio como insumos que permitan la adherencia de estilos de vida saludables por parte del adulto mayor para el control de la hipertensión arterial. En la segunda se estableció el plan de trabajo por medio de acciones proporcionadas por la comunidad; y en la tercera etapa se dio la reflexión de los resultados obtenidos. RESULTADOS: A partir de la apropiación de los recursos agrícolas, el fortalecimiento del baile como actividad física, la incorporación de estrategias motivacionales, el soporte de instituciones que le apuestan al bienestar del adulto mayor y el empoderamiento de los facilitadores; se logra una intervención eficaz y sostenible para el cuidado de la salud del adulto mayor. CONCLUSIONES: Las intervenciones encaminadas a promover la adherencia de estilos de vida saludables en los adultos mayores, deben incluir y preservar el contexto de la comunidad de la cual ellos son parte, donde los recursos de la misma sean los insumos que permitan la promoción de la salud.

The pursuit of healthier communities through a community health medical education program.

BACKGROUND: Distinct periods in the community health undergraduate medical program at the University of La Sabana (Colombia) were identified in its evolution from 1999 to 2013. We describe each period and explain the succesion of changes toward improvement. METHODS: An ordered review of the community health program was constructed based on the retrospective recollection, classification, and analysis of information from document archives and interviews with participants. The review of the experience reconstructs periods of the program, organizing the evolution of its learned lessons and identified changes across the development of community health projects (CHPs) and the phases followed in their implementation. RESULTS: Two principal stages were identified, the first when students' CHPs involved only schools, and the second when students worked in a broader array of community settings. Identified phases of the community health cycle leading to identifying changes across the program timeline were focus of the community-campus partnership; development of relationships among participants; health and health determinants' assessment; defining project goals and objectives; devising a project activity plan; implementing and gathering results; disseminating project achievements; and building sustainability of program activities. Periods were bounded by important new characteristics introduced in the pursuit of healthier communities. DISCUSSION: Understanding the evolution of the program revealed the key concepts and practices in setting community health apprenticeship scenarios for the various participants. Overall, trust and commitment from stakeholders requires competent facilitators able to build meaningful and sustainable collaborations that can translate the purpose of community health practice into an effective teaching-learning experience. Institutional capacity building and collaborative practice contribute to improvements in the community health program and its ability to be flexible to adapt to different contexts. Periods reflecting improvement in this school's programs over time can help others identify key elements that need to be integrated into a community health medical education program.

Performance of the Obstetric Early Warning Score in critically ill patients for the prediction of maternal death.

BACKGROUND: Every day, about 830 women die worldwide from preventable causes related to pregnancy and childbirth. Obstetric early warning scores have been proposed as a potential tool to reduce maternal morbidity and mortality, based on the identification of predetermined abnormal values in the vital signs or laboratory parameters, to generate a rapid and effective medical response. Several early warning scores have been developed for obstetrical patients, but the majority are the result of a clinical consensus rather than statistical analyses of clinical outcome measures (ie, maternal deaths). In 2013, the Intensive Care National Audit and Research Center Case Mix Program reported the first statistically validated early warning scoring system for pregnant women. OBJECTIVE: We sought to assess the performance of the Intensive Care National Audit and Research Center Obstetric Early Warning Score in predicting death among pregnant women who required admission to the intensive care unit. STUDY DESIGN: This retrospective cohort study included pregnant women admitted to the intensive care unit at a tertiary referral center from January 2006 through December 2011 in Colombia, a developing country, with direct and indirect obstetric-related conditions. The Obstetric Early Warning Score was calculated based on data collected during the first 24 hours of intensive care unit admission. The Obstetric Early Warning Score is calculated based on values of the following variables: systolic and diastolic blood pressure, respiratory rate, heart rate, fraction of inspired oxygen (FiO2) required to maintain an oxygen saturation ≥96%, temperature, and level of consciousness. The performance of the Obstetric Early Warning Score was evaluated using the area under the receiver operator characteristic curve. Outcomes selected were: maternal death, need for mechanical ventilation, and/or vasoactive support. Statistical methods included distribution appropriate univariate analyses and multivariate logistic regression. RESULTS: During the study period, 50,897 births were recorded. There were 724 obstetric admissions to critical care, for an intensive care unit admission rate of 14.22 per 1000 deliveries. A total of 702 women were included in the study, with 29 (4.1%) maternal deaths, and a mortality ratio of 56.98 deaths per 100,000 live births. The most frequent causes of admission were direct, obstetric-related conditions (n = 534; 76.1%). The Obstetric Early Warning Score value was significantly higher in nonsurvivors than in survivors [12 (interquartile range 10-13) vs 7 (interquartile range 4-9); P < .001]. Peripartum women with normal values of Obstetric Early Warning Score had 0% mortality rate, while those with high Obstetric Early Warning Score values (>6) had a mortality rate of 6.3%. The area under the receiver operator characteristic curve of the Obstetric Early Warning Score in discrimination of maternal death was 0.84 (95% confidence interval, 0.75-0.92). The overall predictive value of the Obstetric Early Warning Score was better when the main cause of admission was directly related to pregnancy or the postpartum state. The area under the receiver operator characteristic curve of the score in conditions directly related to pregnancy and postpartum was 0.87 (95% confidence interval, 0.79-0.95), while in indirectly related conditions the area under the receiver operator characteristic curve was 0.77 (95% confidence interval, 0.58-0.96). CONCLUSION: Although there are opportunities for improvement, Obstetric Early Warning Score obtained upon admission to the intensive care unit can predict survival in conditions directly related to pregnancy and postpartum. The use of early warning scores in obstetrics may be a highly useful approach in the early identification of women at an increased risk of dying.

Coinfección por virus de inmunodeficiencia humana y micobacterias en un hospital universitario de alta complejidad en Colombia / Co-infection with human immunodeficiency virus and mycobacteria in a tertiary referral hospital in Colombia

Antecedentes: La coinfección por virus de inmunodeficiencia humana (VIH) y micobacterias tiene un efecto dual; el riesgo de enfermedad extrapulmonar y diseminada por micobacterias se incrementa y la progresión de la enfermedad VIH se acelera. La tuberculosis (TB) es la entidad que más amenaza la vida en estos pacientes. Metodología: Estudio observacional, descriptivo, retrospectivo. Objetivo: Determinar las características epidemiológicas, clínicas y el perfil de resistencia en los pacientes con coinfección VIH y micobacterias. Resultados: De 159 pacientes con diagnóstico de infección por VIH, 44 (27,7%) tenían coinfección por micobacterias. La edad promedio fue de 36,7 años ± 11,3. El 86% fueron hombres. Al ingreso 66% tenía síndrome de inmunodeficiencia adquirida (SIDA), 20% historia de TB y 11% historia de otras enfermedades de transmisión sexual. El 50% tenía linfocitos T CD4 <50/mm³. Las principales comorbilidades fueron neoplasias hematológicas 11%, insuficiencia cardiaca 4,5% y enfermedad linfoproliferativa 4,5%. La incidencia anual fue de 4,6%. Las formas diseminadas fueron el 54% de los casos. Se identificó M. tuberculosisen 77,3%, micobacterias no tuberculosas (MNT) en 18,3% y en el 4,5% no fue posible establecerla especie. El 2,5% de los aislamientos de M. tuberculosis fueron multifarmacorresistentes (TB-MDR) y en 18,2% se identificó resistencia a un solo fármaco o resistencia combinada. En el 54%de los pacientes se documentó compromiso pulmonar. La letalidad fue del 9,1%. Conclusión: En nuestros pacientes, la infección por M. tuberculosis es la más frecuente y es llamativa la alta prevalencia de resistencia al menos a un fármaco y la TB-MDR. Las infecciones por MNT son cada vez más frecuentes. Las formas diseminadas y extrapulmonares son muy importantes. La incidencia anual es alta.

Background: Co-infection with the human inmunodeficiency virus (HIV) and mycobacteria hasa synergistic effect; the risk of extrapulmonary and disseminated mycobacterium disease isincreased and the progression of HIV disease is accelerated. Tuberculosis (TB) is the entity thatis most life threatening to these patients. Methodology: Observational, descriptive and retrospective study. Objective: To determine the epidemiological and clinical characteristics and the resistanceprofile in patients coinfected with HIV and mycobacteria. Results: Of 159 patients diagnosed with HIV, 44 (27.7%) patients were co-infected with myco-bacteria. The average age was 36.7 years ± 11.3. Some 86% were men. At admission, 66% hadacquired immune deficiency syndrome (AIDS), 20% a history of TB and 11% a history of othersexually transmitted diseases. A total of 50% reported a count of CD4 lymphocytes less than50 cells/mm 3 . Major comorbidities were haematological malignancies in 11%, heart failure in4.5%, and lymphoproliferative disease in 4.5%. The annual incidence was 4.6%. Disseminatedforms were found in 54% of cases. M. tuberculosis was identified in 77.3%, non-tuberculousmycobacteria (NTM) in 18.3% and in 4.5%, it was not possible to establish the species. Some2.5% of isolates of M. tuberculosis were multidrug-resistant (MDR-TB) and 18.2% were resistantto a single drug or had combined resistance. There was documented pulmonary involvement In54% of patients. Mortality was 9.1%. Conclusion: In our patients, infection with M. tuberculosis was the most common and the highprevalence of resistance to at least one drug and MDR-TB was striking. NTM infections are becoming more common. The extra-pulmonary and disseminated forms are common, and annual incidence is high.

Bacteremia por Staphylococcus coagulasa negativo con concentración inhibitoria mínima para vancomicina ≥ 2 / Coagulase-negative Staphylococcus bacteremia with Vancomycin minimum inhibitory concentrations ≥ 2

Introducción: La bacteremia por Staphylococcus coagulasa negativo (SCN) es una infección que actualmente genera significativa morbimortalidad dado el tipo de pacientes a los que afecta y el cambio en el perfil de susceptibilidad a los antibióticos. Objetivo: Describir características de los pacientes con aislamientos de SCN en sangre con CIM para vancomicina ≥2. Metodología: Estudio observacional descriptivo en pacientes hospitalizados ≥ 15 años. Resultados: De 130 aislamientos, 38 (29,23%) tenían CIM para vancomicina ≥ 2. La mediana de edad fue de 54 años y la razón hombre:mujer, de 1,37:1. Las especies más frecuentes fueron: S. epidermidis (71,1%), S. haemolyticus (13,2%) y S. hominis (7,9%). El 44,7% tenían antecedentes de inmunosupresión, siendo las más importantes: neoplasias (28,9%), enfermedad reumatológica (5,2%) y VIH (2,6%). El 81,5% tenían accesos vasculares, el 97,3% antecedente de hospitalización previa y el 60,5% habían requerido manejo en la UCI. El 81,4% tuvieron exposición previa a antibióticos, y los más utilizados fueron: betalactámicos (78,9%) y vancomicina (50%). En el 2,6% se documentó endocarditis infecciosa. De los aislamientos de SCN con CIM para vancomicina ≥2 µg/ml, el 26,3% fueron sensibles a meticilina. Los principales tratamientos recibidos fueron: daptomicina (31,5%), vancomicina (21%), linezolid (15,7%) y betalactámicos (10,5%). Se utilizó terapia combinada en el 10,5%. La mortalidad general fue del 15,8%, y la mortalidad atribuible, del 33,3%. Conclusión: Un porcentaje considerable de aislamientos tenían heterorresistencia para vancomicina. La bacteremia estuvo asociada con accesos vasculares, hospitalizaciones previas, tratamientos en cuidado intensivo y exposición previa a antibióticos. La inmunosupresión es la comorbilidad más importante, y la mortalidad es significativa.

Introduction: Currently, coagulase-negative Staphylococcus (CNS) bacteremia is an infection that leads to significant morbidity and mortality given the type of patients affected and the recent changes in antimicrobial susceptibility. Objective: To describe the characteristics of patients with CNS blood isolates with vancomycin MIC ≥ 2. Methodology: Descriptive observational study on hospitalised patients ≥ 2 15 years of age. Results: Of 130 isolates, 38 (29,23%) contained vancomycin MIC ≥ 2. The median age was 54 years and the male:female ratio was 1.37:1. The most frequent species were S. epidermidis (71.1%), S. haemolyticus (13.2%) and S. hominis (7.9%). Some 44.7% of patients had a history of immunosuppression, including: neoplasms (28.9%), rheumatologic disease (5.2%) and HIV (2.6%). Some 81,5% had vascular access; 97.3% had previous hospitalisations and 60.5% had required intensive care. A total of 81.4% of the patients had prior exposure to antibiotics and the most commonly used were beta-lactams (78.9%) and vancomycin (50%). Infective endocarditis was documented in 2.6%. Of the CNS isolates with vancomycin MIC ≥ 2, 26.3% were sensitive to methicillin. The main treatments received were: daptomycin (31.5%), vancomycin (21%), linezolid (15.7%) and betalactams (10.5%). Combined therapy was performed in 10.5%. The overall mortality was 15.8% and attributable mortality was 33.3%. Conclusion: A significant proportion of isolates were hetero-resistant to vancomycin. The bacteremia was associated with vascular access, previous hospitalisations, intensive care treatments and prior antibiotic exposure. Immunosuppression is the most important comorbidity and mortality is significant.

Necesidades de formación para el desarrollo integral de la primera infancia: perspectiva desde un estudio regional / Training needs for comprehensive early childhood development: perspective from a regional study

Objetivo: Caracterizar las fortalezas y debilidades de los programas de formación de profesionales de la salud para el desarrollo de competencias en cuidado de la salud para el desarrollo integral (CSDI) de niños menores de 6 años, en 16 municipios de Cundinamarca y Boyacá (Colombia). Métodos: Estudio descriptivo mixto (cuantitativo y cualitativo) mediante entrevistas (n=126) y grupos de discusión (n=3) con profesionales en formación, entrevistas a informantes clave (n=3), revisión de contenidos de planes de estudio (n=9), encuestas a profesionales de la salud (n=111) y acompañantes (n=111) y revisión de historias clínicas (n=54) relacionadas con la atención de niños menores de 6 años. Los resultados se analizaron según cuatro dominios: Preparación para la vida, Prácticas cotidianas para el cuidado de la salud, Seguridad e integridad corporal y Educación inicial. Resultados: Se destaca la inclusión de contenidos sobre preparación para la vida y prácticas cotidianas para el cuidado de la salud, como también la orientación del currículo hacia la atención integral; así como la ausencia de contenidos sobre seguridad e integridad corporal y sobre educación inicial. Un elemento importante de la atención integral hace referencia al conocimiento de los signos de alarma por parte de las familias; pero un 40 % de estas familias no han recibido esta información por parte de profesionales de la salud. Por último, el entorno, redes de apoyo y otras vulnerabilidades son poco exploradas en los servicios de salud. Conclusiones: En programas de formación en salud se destaca la orientación hacia la atención integral. Atención integral que se puede fortalecer en el profesional de la salud en formación desde las capacidades del ser y saber.

Objective: To characterize the strengths and weaknesses of the training programs of health professionals for the development of skills in Healthcare for Integral Development (HID) in early childhood, in 16 municipalities of Cundinamarca and Boyacá (Colombia). Methods: A mixed-methods descriptive study (quantitative and qualitative), carried out through interviews (n = 126) and focus groups (n = 3) with health professionals in training, key informant interviews (n = 3), revision of curriculum content (n = 9), surveys of health professionals (n = 111) and companions (n = 111), and medical record review (n = 54), related to the care of early childhood. The results were analyzed according to four domains: preparation for life, everyday practices for health care, safety and bodily integrity and early education. Results: It is highlighted the inclusion of content on life skills and daily practices for health care, as well as the orientation of the curriculum toward comprehensive care; and the absence of contents on security and bodily integrity, and early education. An important element of comprehensive care refers to the knowledge of the warning signs on the part of families, but 40 % of these families have not received this information from health professionals. Finally, the environment, support networks and other vulnerabilities are little explored in health services. Conclusions: It is highlighted the orientation toward comprehensive care in health training programs. Comprehensive care can strengthen the health professional training from the capabilities of being and knowing.

Reflexiones sobre el concepto de salud comunitaria y consideraciones para su aplicación / Reflections on the concept of community health and considerations for application

Aproximarse a la salud comunitaria exige precisar conceptos como salud y comunidad, sus relaciones, y el contexto en el que ocurren, pues estos conceptos condicionan formas específicas de intervención. La medicina moderna está estructurada sobre las ciencias positivistas que se han desarrollado a partir de la revolución científica de los siglos XV y Las intervenciones de promoción de la salud también están condicionadas por los conceptos clásicos de enfermedad, transmisión y riesgo, los cuales orientan las intervenciones comunitarias. Los sistemas de atención sanitaria están llamados a pasar de un enfoque asistencialista y curativo, hacia un enfoque preventivo y promotor de la salud y la vida, como lo menciona la estrategia de Atención Primaria en Salud. Para ello es crucial revisar el concepto de salud imperante, a la luz de una flexibilización o apertura epistemológica.

Approaching community health requires to clarify concepts such as health and community, its relationships, and the context in which they occur, as these concepts determine specific forms of intervention. Modern medicine is structured on the positivist science developed from the scientific revolution of the fifteenth and sixteenth centuries. The interventions of health promotion are also conditioned by the classical concepts of disease, transmission and risk, which guide community interventions. The health care systems are called to move from a welfare and curative approach to a preventive approach and promoting health and life, as mentioned by the strategy of Primary Health Care. It is crucial to review the concept of health prevailing, in the light of an easing or epistemological opening.

Construcción de entornos saludables en el marco de la Atención Primaria en Salud: el caso de la enseñanza de Salud Comunitaria en el Programa de Medicina de la Universidad de La Sabana / Assembling healthy environments under a Primary Health Care framework: the teachinglearning experience of community health at Universidad de La Sabana undergraduate medical program

Este artículo presenta la reflexión de una experiencia educativa de salud comunitaria en el marco de la Atención Primaria en Salud (APS) en el Programa de Medicina de la Universidad de La Sabana; proceso que articula a estudiantes, ciudadanos y gestores de equipos interdisciplinarios para trabajar por la salud de la población a través del apoyo a la gestión de "entornos saludables". Se profundiza en los elementos que han caracterizado el ejercicio de enseñanza y aprendizaje en APS, el proceso de construcción del programa académico, la confluencia de la Salud Pública y la Medicina Familiar en la APS, la orientación pedagógica de las actividades docente - asistenciales hacia la formulación y ejecución de planes de mejoramiento en salud, proyectos de salud comunitaria y el fomento de entornos saludables en las distintas organizaciones y comunidades abordadas, y se describe la evolución del proceso. Por último, se concluye resaltando aquellos aspectos estratégicos en las competencias médicas y de otros profesionales de la salud necesarios para fortalecer la salud de la población a través de este tipo de programas, y se destaca la importancia de la APS en los procesos de docencia, investigación, proyección social y su articulación con otros sectores afines a la iniciativa.

This article presents the reflection of an education experience in community health, within the framework of Primary Health Care (PHC) in the program of Medicine at Universidad de La Sabana, a process that articulates students, citizens and managers of interdisciplinary teams to work for populations’ health collaborating in the making of "healthy environments". It delves into the elements that have characterized the experience of teaching and learning in PHC, the process of building the academic program, the confluence of Public Health and Family Medicine in PHC, the pedagogical orientation of theoretical and practical activities in health care education, the formulation and implementation of improvement plans in health, community health projects and promoting healthy environments in different organizations and communities, while describing the evolution of the process. Conclusions emphasize strategic areas necessary in physicians and other health professionals, competencies necessary to strengthen population health through this kind of programs, stressing the importance of PHC and healthy environments in teaching, researching, and social action in coordination with other sectors related to the initiative.

Infección por VIH posterior a exposición ocupacional de riesgo biológico en trabajadores de la salud / HIV infection after biohazard occupational exposure to HIV in health care

Se describe el caso de una mujer auxiliar de enfermería quien sufrió accidente de riesgo biológico luego de punción con aguja, al canalizar una vena periférica. La auxiliar de enfermería era residente en área rural y fue atendida en urgencias de su hospital local. El origen de la exposición (fuente) fue positivo para VIH y negativo para VHB y VHC. La trabajadora de la salud accidentada fue negativa para VIH, VHB y VHC y no recibió profilaxis antirretroviral (ARV) postexposición y luego fue remitida a nuestra institución. Este es el primer reporte en Colombia de seroconversión al VIH luego de exposición ocupacional.

We report a postexposure HIV infection in a woman nursing assistant, resident in rural area. She suffered biohazard exposure after needlestick incident during channeling of peripheral vein. She was attended at their local hospital emergency room. The serostatus on the patient's exposure source (source) was HIV positive and negative for HBV and HCV. The health worker was negative for HIV, HBV and HCV and she did not receive antiretroviral (ARV) prophylaxis at the initial consultation. Thereafter she was referred to our institution. This is the first report of HIV infection after occupational exposure in Colombia.

Maternal mortality secondary to acute respiratory failure in Colombia: a population-based analysis.

PURPOSE: To estimate the mortality rate and trends of respiratory failure in the pregnant and postpartum population of Colombia. METHODS: A retrospective analysis of the national registry of mortality in Colombia was performed from 1998 to 2009. Maternal death was defined as death that occurred during pregnancy or up to 42 days postpartum. Two independent investigators reviewed maternal deaths to determine deaths caused by respiratory failure. Inter-rater agreement was assessed by kappa correlation coefficient. Causes of respiratory failure were identified according to the International Classification of Diseases (ICD-10). RESULTS: During the study period, 8,637,486 live births were reported with 6,676 maternal deaths for an overall maternal mortality rate (MMR) of 82.9 per 100,000 live births. Of these, a total of 835 cases were related to respiratory failure, with a specific MMR of 9.69 per 100,000 live births. The main causes of maternal deaths due to respiratory failure included pulmonary sepsis (284 cases, or 3.58 per 100,000 live births), pulmonary embolism (119 cases or 1.50 per 100,000 live births), and preeclampsia-related pulmonary edema (112 cases or 1.41 per 100,000 live births). All-cause maternal mortality ratio decreased yearly from 1998 to 2009 by -3.76% (95% CI -4.83 to -2.67), while the trend of mortality secondary to respiratory failure remained stable over time (P = 0.449). CONCLUSIONS: Respiratory failure is an important cause of mortality in the obstetric population in Colombia, with pulmonary sepsis as the lead cause of respiratory failure among maternal deaths. While overall maternal mortality rates have decreased in the last decade, respiratory failure-related deaths have remained stable over time.