Presence and Role of the Type 3 Fimbria in the Adherence Capacity of Enterobacter hormaechei subsp. hoffmannii.

Enterobacter hormaechei, one of the species within the Enterobacter cloacae complex, is a relevant agent of healthcare-associated infections. In addition, it has gained relevance because isolates have shown the capacity to resist several antibiotics, particularly carbapenems. However, knowledge regarding colonization and virulence mechanisms of E. hormaechei has not progressed to the same extent as other Enterobacteriaceae species as Escherichia coli or Klebsiella pneumoniae. Here, we describe the presence and role of the type 3 fimbria, a chaperone-usher assembled fimbria, which was first described in Klebsiella spp., and which has been detected in other representatives of the Enterobacteriaceae family. Eight Chilean E. cloacae isolates were examined, and among them, four E. hormaechei isolates were found to produce the type 3 fimbria. These isolates were identified as E. hormaechei subsp. hoffmannii, one of the five subspecies known. A mutant E. hormaechei subsp. hoffmannii strain lacking the mrkA gene, encoding the major structural subunit, displayed a significantly reduced adherence capacity to a plastic surface and to Caco-2 cells, compared to the wild-type strain. This phenotype of reduced adherence capacity was not observed in the mutant strains complemented with the mrkA gene under the control of an inducible promoter. Therefore, these data suggest a role of the type 3 fimbria in the adherence capacity of E. hormaechei subsp. hoffmannii. A screening in E. hormaechei genomes contained in the NCBI RefSeq Assembly database indicated that the overall presence of the type 3 fimbria is uncommon (5.94-7.37%), although genes encoding the structure were detected in representatives of the five E. hormaechei subspecies. Exploration of complete genomes indicates that, in most of the cases, the mrkABCDF locus, encoding the type 3 fimbria, is located in plasmids. Furthermore, sequence types currently found in healthcare-associated infections were found to harbor genes encoding the type 3 fimbria, mainly ST145, ST78, ST118, ST168, ST66, ST93, and ST171. Thus, although the type 3 fimbria is not widespread among the species, it might be a determinant of fitness for a subset of E. hormaechei representatives.

Distribution of papA and papG Variants among Escherichia coli Genotypes: Association with Major Extraintestinal Pathogenic Lineages.

The pyelonephritis-associated fimbria (P fimbria) is one of the most recognized adhesion determinants of extraintestinal pathogenic Escherichia coli strains (ExPECs). Twelve variants have been described for the gene encoding the P fimbria major structural subunit PapA and three variants for the gene encoding the adhesin subunit PapG. However, their distribution among the ExPEC diversity has not been comprehensively addressed. A complete landscape of that distribution might be valuable for delineating basic studies about the pathogenicity mechanisms of ExPECs and following up on the evolution of ExPEC lineages, particularly those most epidemiologically relevant. Therefore, we performed a massive descriptive study to detect the papA and papG variants along different E. coli genotypes represented by genomic sequences contained in the NCBI Assembly Refseq database. The most common papA variants were F11, F10, F48, F16, F12, and F7-2, which were found in significant association with the most relevant ExPEC genotypes, the phylogroups B2 and D, and the sequence types ST95, ST131, ST127, ST69, ST12, and ST73. On the other hand, the papGII variant was by far the most common followed by papGIII, and both were also found to have a significant association with common ExPEC genotypes. We noticed the presence of genomes, mainly belonging to the sequence type ST12, harboring two or three papA variants and two papG variants. Furthermore, the most common papA and papG variants were also detected in records representing strains isolated from humans and animals such as poultry, bovine, and dogs, supporting previous hypotheses of potential cross-transmission. Finally, we characterized a set of 17 genomes from Chilean uropathogenic E. coli strains and found that ST12 and ST73 were the predominant sequence types. Variants F7-1, F7-2, F8, F9, F11, F13, F14, F16, and F48 were detected for papA, and papGII and papGIII variants were detected for papG. Significant associations with the sequence types observed in the analysis of genomes contained in the NCBI Assembly Refseq database were also found in this collection in 16 of 19 cases for papA variants and 7 of 9 cases for the papG variants. This comprehensive characterization might support future basic studies about P fimbria-mediated ExPEC adherence and future typing or epidemiological studies to monitor the evolution of ExPECs producing P fimbria.

Vehicular Visible Light Positioning System Based on a PSD Detector.

In this paper, we explore the use of visible light positioning (VLP) technology in vehicles in intelligent transportation systems (ITS), highlighting its potential for maintaining effective line of sight (LOS) and providing high-accuracy positioning between vehicles. The proposed system (V2V-VLP) is based on a position-sensitive detector (PSD) and exploiting car taillights to determine the position and inter-vehicular distance by angle of arrival (AoA) measurements. The integration of the PSD sensor in vehicles promises exceptional positioning accuracy, opening new prospects for navigation and driving safety. The results revealed that the proposed system enables precise measurement of position and distance between vehicles, including lateral distance. We evaluated the impact of different focal lengths on the system performance, achieving cm-level accuracy for distances up to 35 m, with an optimum focal length of 25 mm, and under low signal-to-noise conditions, which meets the standards required for safe and reliable V2V applications. Several experimental tests were carried out to validate the results of the simulations.

Recent developments in the engineering of Rubisco activase for enhanced crop yield.

Rubisco activase (RCA) catalyzes the release of inhibitory sugar phosphates from ribulose-1,6-biphosphate carboxylase/oxygenase (Rubisco) and can play an important role in biochemical limitations of photosynthesis under dynamic light and elevated temperatures. There is interest in increasing RCA activity to improve crop productivity, but a lack of understanding about the regulation of photosynthesis complicates engineering strategies. In this review, we discuss work relevant to improving RCA with a focus on advances in understanding the structural cause of RCA instability under heat stress and the regulatory interactions between RCA and components of photosynthesis. This reveals substantial variation in RCA thermostability that can be influenced by single amino acid substitutions, and that engineered variants can perform better in vitro and in vivo under heat stress. In addition, there are indications RCA activity is controlled by transcriptional, post-transcriptional, post-translational, and spatial regulation, which may be important for balancing between carbon fixation and light capture. Finally, we provide an overview of findings from recent field experiments and consider the requirements for commercial validation as part of efforts to increase crop yields in the face of global climate change.

A Straightforward Cytometry-Based Protocol for the Comprehensive Analysis of the Inflammatory Valve Infiltrate in Aortic Stenosis.

Aortic stenosis (AS) is a frequent cardiac disease in old individuals, characterized by valvular calcification, fibrosis, and inflammation. Recent studies suggest that AS is an active inflammatory atherosclerotic-like process. Particularly, it has been suggested that several immune cell types, present in the valve infiltrate, contribute to its degeneration and to the progression toward stenosis. Furthermore, the infiltrating T cell subpopulations mainly consist of oligoclonal expansions, probably specific for persistent antigens. Thus, the characterization of the cells implicated in the aortic valve calcification and the analysis of the antigens to which those cells respond to is of utmost importance to develop new therapies alternative to the replacement of the valve itself. However, calcified aortic valves have been only studied so far by histological and immunohistochemical methods, unable to render an in-depth phenotypical and functional cell profiling. Here we present, for the first time, a simple and efficient cytometry-based protocol that allows the identification and quantification of infiltrating inflammatory leukocytes in aortic valve explants. Our cytometry protocol saves time and facilitates the simultaneous analysis of numerous surface and intracellular cell markers and may well be also applied to the study of other cardiac diseases with an inflammatory component.

Accuracy and Precision of Agents Orientation in an Indoor Positioning System Using Multiple Infrastructure Lighting Spotlights and a PSD Sensor.

In indoor localization there are applications in which the orientation of the agent to be located is as important as knowing the position. In this paper we present the results of the orientation estimation from a local positioning system based on position-sensitive device (PSD) sensors and the visible light emitted from the illumination of the room in which it is located. The orientation estimation will require that the PSD sensor receives signal from either 2 or 4 light sources simultaneously. As will be shown in the article, the error determining the rotation angle of the agent with the on-board sensor is less than 0.2 degrees for two emitters. On the other hand, by using 4 light sources the three Euler rotation angles are determined, with mean errors in the measurements smaller than 0.35° for the x- and y-axis and 0.16° for the z-axis. The accuracy of the measurement has been evaluated experimentally in a 2.5 m-high ceiling room over an area of 2.2 m2 using geodetic measurement tools to establish the reference ground truth values.

Intermediate-Grade Teachers' Adaptations for Weaker Writers: A National Survey in Urban Schools in Chile.

Two hundred fifty-four intermediate-grade teachers (88% female) in urban public schools in Chile indicated how frequently they made writing instructional adaptations for their weakest writers, which included students with learning and other disabilities. A majority of teachers reported making the following adaptations for their weakest writers at least once a week: individual tutoring, assistance from a peer when writing, and extra instruction in grammar/spelling, planning/revising, handwriting, sentence construction, and text structure. On a monthly basis or more often, a majority of teachers reported they provided weaker writers with choice about writing assignments, the opportunity to complete an alternative writing assignment, and extra writing instruction via computer technology. How often an adaptation was applied did not differ by grade. The perceived adequacy of teacher undergraduate preparation to teach writing, their efficacy to teach writing, and the proportion of students with disability in their classes each made a unique and statistically significant contribution to predicting how frequently teachers applied adaptations for their weakest writers. The Chilean teachers in this study reportedly made adaptations for weaker writers in their class more often than teachers in studies conducted in other countries, including the United States.

Weak Calibration of a Visible Light Positioning System Based on a Position-Sensitive Detector: Positioning Error Assessment.

Reduced deployment and calibration requirements are key for scalable and cost-effective indoor positioning systems. In this work, we propose a low-complexity, weak calibration procedure for an indoor positioning system based on infrastructure lighting and a positioning-sensitive detector. The proposed calibration relies on genetic algorithms to obtain the relevant system parameters in the real positioning environment without a priori information, and requires a low number of simple measurements. The achievable performance of the proposal was assessed by direct comparison with a formal offline calibration method requiring complex dedicated infrastructure and instruments. The comparative error assessment showed that the maximum accuracy reduction compared to the significantly more costly formal calibration was below 25 mm, and the overall absolute positioning error was smaller than 35 mm with orientation errors of around 0.25°. The performance achieved with the proposed weak calibration procedure is sufficient for many indoor positioning applications and largely reduces the cost and complexity of setting up the positioning system in real environments.

Effects of Progressed and Nonprogressed Volume-Based Overload Plyometric Training on Components of Physical Fitness and Body Composition Variables in Youth Male Basketball Players.

ABSTRACT: Palma-Muñoz, I, Ramírez-Campillo, R, Azocar-Gallardo, J, Álvarez, C, Asadi, A, Moran, J, and Chaabene, H. Effects of progressed and nonprogressed volume-based overload plyometric training on components of physical fitness and body composition variables in youth male basketball players. J Strength Cond Res 35(6): 1642-1649, 2021-This study examined the effect of 6 weeks of progressed and nonprogressed volume-based overload plyometric training (PT) on components of physical fitness and body composition measures in young male basketball players, compared with an active control group. Subjects were randomly assigned to a progressed PT (PPT, n = 7; age = 14.6 ± 1.1 years), a non-PPT (NPPT, n = 8, age = 13.8 ± 2.0 years), or a control group (CG, n = 7, age = 14.0 ± 2.0 years). Before and after training, body composition measures (muscle mass and fat mass), countermovement jump with arms (CMJA) and countermovement jump without arms (CMJ), horizontal bilateral (HCMJ) and unilateral jump with right leg (RJ) and left leg (LJ), 20-cm drop jump (DJ20), sprint speed (10 m sprint), and change of direction speed (CODS [i.e., T-test]) were tested. Significant effects of time were observed for muscle and fat mass, all jump measures, and CODS (all p < 0.01; d = 0.37-0.83). Significant training group × time interactions were observed for all jump measures (all p < 0.05; d = 0.24-0.41). Post hoc analyses revealed significant pre-post performance improvements for the PPT (RJ and LJ: ∆18.6%, d = 0.8 and ∆22.7%, d = 0.9, respectively; HCMJ: ∆16.4%, d = 0.8; CMJ: ∆22.4%, d = 0.7; CMJA: ∆23.3%, d = 0.7; and DJ20: ∆39.7%, d = 1.1) and for the NPPT group (LJ: ∆14.1%, d = 0.4; DJ20: ∆32.9%, d = 0.8) with greater changes after PPT compared with NPPT for all jump measures (all p < 0.05; d = 0.21-0.81). The training efficiency was greater (p < 0.05; d = 0.22) after PPT (0.015% per jump) compared with NPPT (0.0053% per-jump). The PPT induced larger performance improvements on measures of physical fitness as compared to NPPT. Therefore, in-season progressive volume-based overload PT in young male basketball players is recommended.

Smart Video Surveillance System Based on Edge Computing.

New processing methods based on artificial intelligence (AI) and deep learning are replacing traditional computer vision algorithms. The more advanced systems can process huge amounts of data in large computing facilities. In contrast, this paper presents a smart video surveillance system executing AI algorithms in low power consumption embedded devices. The computer vision algorithm, typical for surveillance applications, aims to detect, count and track people's movements in the area. This application requires a distributed smart camera system. The proposed AI application allows detecting people in the surveillance area using a MobileNet-SSD architecture. In addition, using a robust Kalman filter bank, the algorithm can keep track of people in the video also providing people counting information. The detection results are excellent considering the constraints imposed on the process. The selected architecture for the edge node is based on a UpSquared2 device that includes a vision processor unit (VPU) capable of accelerating the AI CNN inference. The results section provides information about the image processing time when multiple video cameras are connected to the same edge node, people detection precision and recall curves, and the energy consumption of the system. The discussion of results shows the usefulness of deploying this smart camera node throughout a distributed surveillance system.

Machine-learning model led design to experimentally test species thermal limits: The case of kissing bugs (Triatominae).

Species Distribution Modelling (SDM) determines habitat suitability of a species across geographic areas using macro-climatic variables; however, micro-habitats can buffer or exacerbate the influence of macro-climatic variables, requiring links between physiology and species persistence. Experimental approaches linking species physiology to micro-climate are complex, time consuming and expensive. E.g., what combination of exposure time and temperature is important for a species thermal tolerance is difficult to judge a priori. We tackled this problem using an active learning approach that utilized machine learning methods to guide thermal tolerance experimental design for three kissing-bug species: Triatoma infestans, Rhodnius prolixus, and Panstrongylus megistus (Hemiptera: Reduviidae: Triatominae), vectors of the parasite causing Chagas disease. As with other pathogen vectors, triatomines are well known to utilize micro-habitats and the associated shift in microclimate to enhance survival. Using a limited literature-collected dataset, our approach showed that temperature followed by exposure time were the strongest predictors of mortality; species played a minor role, and life stage was the least important. Further, we identified complex but biologically plausible nonlinear interactions between temperature and exposure time in shaping mortality, together setting the potential thermal limits of triatomines. The results from this data led to the design of new experiments with laboratory results that produced novel insights of the effects of temperature and exposure for the triatomines. These results, in turn, can be used to better model micro-climatic envelope for the species. Here we demonstrate the power of an active learning approach to explore experimental space to design laboratory studies testing species thermal limits. Our analytical pipeline can be easily adapted to other systems and we provide code to allow practitioners to perform similar analyses. Not only does our approach have the potential to save time and money: it can also increase our understanding of the links between species physiology and climate, a topic of increasing ecological importance.

Estimación del dolor durante el perioperatorio: Una revisión narrativa de los dispositivos disponibles / Estimative pain measurement during perioperative period: a review of current available devices

Pain is a complex subjective organic function which is influenced by sensorial, emotional, cognitive and behavioral elements. Despite the wide offer of pain measurement devices in the perioperative period, none of them is completely validated for their transverse use in the anesthetic practice. The aim of this review is to present the existing devices for objective pain evaluation during the perioperative period along with the scientific evidence supporting each of them. Articles from the PubMed/MEDLINE literature search engine were reviewed. As result, 37 articles were selected due to its relevance, from which 13 pain assessment devices were described, regarding its clinical relevance as well as the amount of scientific evidence found. Among them are ANI, NOL, pupillometry, qNOX, and others. The nociceptive measurement performed by most of these is based mainly on the evaluation of the autonomic nervous system activity and variations of the electroencephalographic signal. However, it is not possible to recommend any particular device. This review aims to offer a broad overview of the available options in order to estimate the role that each of them could play in clinical anesthesiology practice.

El dolor es una experiencia subjetiva compleja en la que inciden elementos sensoriales, emocionales, cognitivos y conductua- les. A pesar de una amplia oferta de dispositivos para medir dolor en el perioperatorio, hoy no existe un instrumento de medición de analgesia validado y utilizado transversalmente en la práctica anestésica. El objetivo de esta revisión es presentar las actuales opciones disponibles para la medición del dolor agudo utilizadas en el período perioperatorio junto con la evidencia científica que respalda cada una de ellas. Se realizó una revisión de la literatura utilizando como fuente de búsqueda bibliográfica la base de datos MEDLINE/pubMed utilizando términos MESH. Como resultado, se seleccionaron 37 artículos de acuerdo a su importancia, a partir de los cuales se describen 13 dispositivos de valoración nociceptiva, a propósito de su relevancia clínica como también por la cantidad de evidencia científica encontrada. Entre ellos destacan ANI, NOL, pupilometría, qNOX, entre otros. La medición nociceptiva realizada por la mayoría de estos se basa principalmente en la evaluación de la actividad del sistema nervioso autónomo y variaciones de la señal electroencefalográfica. Sin embargo, no es posible recomendar algún dispositivo en particular. Esta revisión pretende ofrecer una visión amplia de las opciones disponibles con el fin de estimar el rol que cada uno de ellos podría desempeñar en la práctica clínica anestesiológica.

Accuracy and Precision Assessment of AoA-Based Indoor Positioning Systems Using Infrastructure Lighting and a Position-Sensitive Detector.

Unlike GNSS-based outdoor positioning, there is no technological alternative for Indoor Positioning Systems (IPSs) that generally stands out from the others. In indoor contexts, the measurement technologies and localization strategies to be used depend strongly on the application requirements and are complementary to each other. In this work, we present an optical IPS based on a Position-Sensitive Detector (PSD) and exploiting illumination infrastructure to determine the target position by Angle of Arrival (AoA) measurements. We combine the proposed IPS with different positioning strategies depending on the number of visible emitters (one, two, or more) and available prior or additional information about the scenario and target. The accuracy and precision of the proposal is assessed experimentally for the different strategies in a 2.47 m high space covering approximately 2.2 m2, using high-end geodetic equipment to establish the reference ground truth. When the orientation of the target is known from external measurements, an average positioning error of 8.2 mm is obtained using the signal received from only one emitter. Using simultaneous observations from two emitters, an average positioning error of 9.4 mm is obtained without external information when the target movement is restricted to a plane. Conversely, if four signals are available, an average positioning error of 4.9 cm is demonstrated, yielding the complete 3D pose of the target free of any prior assumption or additional measurements. In all cases, a precision (2σ) better than 5.9 mm is achieved across the complete test space for an integration time of 10 ms. The proposed system represents a prospectively useful alternative for indoor positioning applications requiring fast and reliable cm-level accuracy with moderate cost when smart illumination infrastructure is available in the environment.

Impact of alkaloids in food consumption, metabolism and survival in a blood-sucking insect.

The sense of taste provides information about the "good" or "bad" quality of a food source, which may be potentially nutritious or toxic. Most alkaloids taste bitter to humans, and because bitter taste is synonymous of noxious food, they are generally rejected. This response may be due to an innate low palatability or due to a malaise that occurs after food ingestion, which could even lead to death. We investigated in the kissing bug Rhodnius prolixus, whether alkaloids such as quinine, caffeine and theophylline, are merely distasteful, or if anti-appetitive responses are caused by a post-ingestion physiological effect, or both of these options. Although anti-appetitive responses were observed for the three alkaloids, only caffeine and theophylline affect metabolic and respiratory parameters that reflected an underlying physiological stress following their ingestion. Furthermore, caffeine caused the highest mortality. In contrast, quinine appears to be a merely unpalatable compound. The sense of taste helps insects to avoid making wrong feeding decisions, such as the intake of bitter/toxic foods, and thus avoid potentially harmful effects on health, a mechanism preserved in obligate hematophagous insects.

Analysis of Multiple-Access Discrimination Techniques for the Development of a PSD-Based VLP System.

There are several technologies and techniques available when developing indoor positioning systems (IPS). Recently, the development of positioning systems based on optical signals has aroused great interest, mainly those using visible light from the lighting infrastructure. In this work, we analyze which techniques give better results to lay the foundations for the development of a Visible Light Positioning system (VLP). Working only with a receiver, it is analyzed what the result of determining the position of different emitters is when they emit simultaneously and without any synchronism. The results obtained by Frequency Division Multiple Access (FDMA) (with digital bandpass filters, I/Q demodulation, and FFT) and Code Division Multiple Access (CDMA) are compared. The interference between signals when emitted simultaneously from multiple emitters is analyzed as well as the errors they cause and how these effects can be mitigated. As a result of the research, the advantages and disadvantages using different multiple-access determination techniques are determined. In addition, advantages and disadvantages of using FDMA and CDMA techniques as well as hardware requirements that make one more feasible than the other are presented. The system behavior, in terms of errors, is established using FDMA and different configurations such as: I/Q, RMS, or FFT. The work also determines the error rates that can be obtained with the different FDMA and CDMA configurations, considering different error scenarios and integration time. Synthetic emulations and empirical tests were performed, which concluded that IPS systems based on optical signals and PSD sensors can achieve very high measurement accuracies and a high measurement rate. Obtained positioning errors in a room of 3 m height are less than 1 cm when working in noisy environments.

Bloqueo atrioventricular completo, taquicardia ventricular y muerte súbita en una paciente gestante a término / Complete atrioventricular block, ventricular tachycardia and sudden death in a pregnant woman at term

Resumen Se presenta un caso clínico de una mujer gestante que presentó bloqueo atrioventricular completo en labor de parto, bradicardia severa, intervalo QT prolongado secundario, torsión de puntas y paro cardiorrespiratorio, del cual fue recuperada. Se discuten los mecanismos potenciales que pueden desencadenar este escenario.

Abstract A clinical case of a pregnant woman who presented with complete atrioventricular block in labor, severe bradycardia, secondary prolonged QT interval, Torsades de pointes and aborted cardiorespiratory arrest is presented. Potential mechanisms that can trigger this scenario are discussed.

Clinical manifestations of homozygote allele carriers in Huntington disease.

OBJECTIVE: Because patients homozygous for Huntington disease (HD) receive the gain-of-function mutation in a double dose, one would expect a more toxic effect in homozygotes than in heterozygotes. Our aim was to investigate the phenotypic differences between homozygotes with both alleles ≥36 CAG repeats and heterozygotes with 1 allele ≥36 CAG repeats. METHODS: This was an international, longitudinal, case-control study (European Huntington's Disease Network Registry database). Baseline and longitudinal total functional capacity, motor, cognitive, and behavioral scores of the Unified Huntington's Disease Rating Scale (UHDRS) were compared between homozygotes and heterozygotes. Four-year follow-up data were analyzed using longitudinal mixed-effects models. To estimate the association of age at onset with the length of the shorter and larger allele in homozygotes and heterozygotes, regression analysis was applied. RESULTS: Of 10,921 participants with HD (5,777 female [52.9%] and 5,138 male [47.0%]) with a mean age of 55.1 ± 14.1 years, 28 homozygotes (0.3%) and 10,893 (99.7%) heterozygotes were identified. After correcting for multiple comparisons, homozygotes and heterozygotes had similar age at onset and UHDRS scores and disease progression. In the multivariate linear regression analysis, the longer allele was the most contributing factor to decreased age at HD onset in the homozygotes (p < 0.0001) and heterozygotes (p < 0.0001). CONCLUSIONS: CAG repeat expansion on both alleles of the HTT gene is infrequent. Age at onset, HD phenotype, and disease progression do not significantly differ between homozygotes and heterozygotes, indicating similar effect on the mutant protein. CLASSIFICATION OF EVIDENCE: This study provides Class II evidence that age at onset, the motor phenotype and rate of motor decline, and symptoms and signs progression is similar in homozygotes compared to heterozygotes.

Characterization of Multipath Effects in Indoor Positioning Systems by AoA and PoA Based on Optical Signals.

In this paper, we characterize and measure the effects of the errors introduced by the multipath when obtaining the position of an agent by means of Indoor Positioning Systems (IPS) based on optical signal. These effects are characterized in Local Positioning Systems (LPSs) based on two different techniques: the first one by determining the Angle of Arrival (AoA) of the infrared signal (IR) to the detector; and the second one by working with the measurement of the Phase shift of signal Arrival from the transmitter to a receiver (PoA). We present the obtained results and conclusions, which indicate that using Position Sensitive Devices (PSD) the multipath effects for AoA have little impact on the measurement, while for PoA the positioning errors are very significant, making the system useless in many cases.

FPGA embedded multichannel analyzer.

An multichannel analyzer has been designed, and its performance has been evaluated. The multichannel analyzer is embedded into a Field programmable gate array. The design incudes the virtual instrument in order to hand and to visualize the pulse height spectrum. Two commercially available multichannel analyzers using a NaI(Tl) and HPGe detectors were used to obtain the pulse height spectra of 137Cs, 60Co and 152Eu sources and were compared with the pulse height spectra obtained with the embedded multichannel analyzer, being alike the spectra obtained with the commercial multichannel analyzer. Our design is smaller, low cost and it has options to add other features.

Modeling the Effect of Optical Signal Multipath.

Here, we propose a model to determine the effect of multipath in indoor environments when the shape and characteristics of the environment are known. The main paper goal is to model the multipath signal formation to solve, as much as possible, the negative effects in light communications, as well as the indoor positioning errors due to this phenomenon when using optical signals. The methodology followed was: analyze the multipath phenomenon, establish a theoretical approach and propose different models to characterize the behavior of the channel, emitter and receiver. The channel impulse response and received signal strength are obtained from different proposed algorithms. We also propose steps for implementing a numerical procedure to calculate the effects of these multipaths using information that characterizes the environment, transmitter and receiver and their corresponding positions. In addition, the results of an empirical test in a controlled environment are compared with those obtained using the model, in order to validate the latter. The results may largely vary with respect to the cell size used to discretize the environment. We have concluded that a cell size whose side is 20-times smaller than the minimum distance between emitter and receiver (i.e., 10 cm × 10 cm for a 2-m distance) provides almost identical results between the empirical tests and the proposed model, with an affordable computational load.