ABSTRACT
Babaco (Vasconcellea x heilbornii), a fruit-bearing vegetatively propagated crop native to Ecuador, is appreciated for its distinctive flavor and nutritional properties. The aim of this research was to determine a functional protocol for tissue culture propagation of virus-free babaco plants including in vitro establishment, multiplication, rooting, and acclimation. First, symptomless babaco plants from a single commercial nursery were analyzed for virus detection and cared for using different disinfection treatments in the greenhouse to reduce contamination during the in vitro establishing step, and three cytokinins, 6-(γ,γ-Dimethylallylamino) purine (2IP), 6-Benzylaminopurine (BAP), and Thidiazuron (TDZ), were used to determine the best hormone for multiplication. The best treatment for plant disinfection was the weekly application of copper sulfate at the greenhouse and a laboratory disinfection using ethanol (EtOH) (70%), Clorox (2%), and a solution of povidone iodine (2.5%), with an 80% survival during in vitro plant establishment. TDZ showed a better multiplication rate when compared with other hormones, and 70% of the rooted plants were successfully acclimated at the greenhouse. Generated plants were virus-free when tested against babaco mosaic virus (BabMV) and papaya ringspot virus (PRSV), two of the most important viruses that can affect babaco. An efficient protocol to produce virus-free babaco plants was elaborated with an integrated use of viral diagnostic tools to ensure the production of healthy start material to farmers.
ABSTRACT
Background: Parents seek support on behalf of their transgender and gender nonconforming (TGNC) children. Previous qualitative studies explore the types of support parents seek inside and outside of healthcare settings. Healthcare providers often remain unprepared to effectively provide gender-affirming services to TGNC children and their accompanying parents and may benefit from learning about the support seeking experiences of parents with TGNC children. Aims: This paper summarized qualitative research studies that address the topic of parents seeking support on behalf of their TGNC children. We produced this report for healthcare providers to review to enhance gender-affirming services for parents and TGNC children. Methods: This paper outlines a qualitative metasummary of studies from the United States or Canada with data collected from parents of TGNC children. Data collection included the steps of journal runs, database searches, reference checks, and area scans. Data analysis involved the steps of extracting, editing, grouping, abstracting, and calculating the intensity and frequency effect sizes for finding statements from qualitative research study articles. Results: The results of this metasummary yielded two primary themes, six subthemes, and 24 total findings. The first primary theme of seeking guidance had three subthemes: educational resources, community networks, and advocacy efforts. The second primary theme of seeking healthcare had three subthemes: healthcare providers, mental healthcare, and general healthcare. Discussion: These findings provide information healthcare providers can use to inform their practice. These findings also highlight the importance of providers working collaboratively with parents when serving TGNC children. This article concludes with practical tips for providers.
ABSTRACT
Una de las principales dificultades en la enseñanza de la odontología es el logro de competencias clínicas en compatibilidad con un tratamiento clínico adecuado y enseñanza de principios éticos. Los programas de formación han contemplado el entrenamiento de "requisitos" o procedimientos clínicos a cumplir. Sin embargo, la escasez de pacientes junto con el dilema asociado a la selección de pacientes han motivado el desarrollo de nuevas estrategias en docencia. En el presente estudio se evaluó la percepción de 91 estudiantes del pregrado de la carrera de Odontología de la Pontificia Universidad Católica de Chile, respecto de dos sistemas de evaluación basado en "esquemas clínicos" y en "requisitos clínicos". El estudio contempló la aplicación de encuestas y de focus group. Los resultados mostraron una amplia preferencia por el sistema de "esquemas clínicos". Dentro de los elementos destacados por los estudiantes se indicó un mejor ambiente de trabajo, reducción del estrés y aplicación de principios éticos. Entre las desventajas del sistema de "esquemas clínicos" se mencionó una mayor dificultad para el entrenamiento de destrezas clínicas específicas. Estudios futuros deberían evaluar la aplicación del sistema de evaluación en el contexto de diferentes disciplinas de la odontología y generar modificaciones minimizando sus desventajas.
One of the main difficulties in dental education is teaching clinical skills in conjunction with patient treatments and the development of solid ethical principles. Traditionally, clinical training programs consisted of teaching many different "requirements" or clinical procedures. However, the limited number of patients presenting the required clinical conditions to teach these procedures, as well as the ethical dilemma of as the selection of patients for educational purposes have motivated the development of new strategies for clinical teaching. This study assessed the perception of 91 undergraduate students of the Dentistry Program of Pontificia Universidad Católica de Chile, who compared two evaluation methods; one based on "clinical schemes" and the other based on more traditional specific clinical activities. Students who participated in this study answered surveys and participated in focus groups. Results showed a high preference for the evaluation method based on "clinical schemes". According to student's perceptions, important features were improved with this new method, such as working environment, stress reduction, and the application of ethical principles with patients. Disadvantages mentioned by the students included a greater difficulty to practice certain specific clinical skills. Future studies should evaluate this new teaching method within different dentistry disciplines and generate modifications to reduce the disadvantages found in this new system.
ABSTRACT
Los niños menores de 3 años son llevados a la consulta médica de forma rutinaria. Sin embargo, la consulta odontológica es muy infrecuente a esta edad. Por otro lado, los elementos que median en la decisión de consultar o no al dentista en esta etapa son hasta el momento poco conocidos. El objetivo de este estudio cualitativo fue identificar imaginarios, creencias y actitudes que podrían intervenir en la decisión de realizar una consulta odontopediátrica en pacientes menores de 3 años. Se aplicaron 10 entrevistas semi-estructuradas a mujeres profesionales, madres de niños entre 8 y 36 meses. Luego, se realizaron 4 entrevistas grupales en profundidad a 13 mujeres que no habían participado en la entrevista inicial. A pesar del alto nivel educativo de las madres se detectó un importante desconocimiento en temas de salud oral. Las madres sub-dimensionan el potencial de la consulta odontopediátrica en esta etapa. En segundo lugar, las madres consideran que una consulta odontopediátrica en este período es abrumadora considerando las altas demandas de esta etapa de la maternidad. Es importante mejorar los conocimientos de salud oral en este grupo de la población y modificar estrategias de prevención y promoción de la salud en preescolares.
Parents of children under 3 years old frequently take their children to medical check-ups. However, dental check-ups are infrequent at this age. The reasons behind this behavior are not well known. The present qualitative study aimed to identify social imaginary, attitudes and beliefs that may influence the decision to consult a pediatric dentist in parents of children under 3 years old. Semi-structured interviews were applied to 10 working mothers that had children between 8 and 36 months. These interviews allowed the identification of the most significant issues to be explored in this study. Afterwards, a total of 13 mothers who did not participate in the initial interviews were divided in 4 groups and in-depth group interviews were performed. Mothers involved in this study had a high educational level. However, their knowledge concerning oral health issues was very poor. Moreover, a frequent finding in this study was the notion that a dental consultation at this stage is considered to be overwhelming by these women because of their very demanding duties . It is important to increase the knowledge regarding oral health topics in this group and to modify policies about oral health at this stage of a child's life.
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Adult , Health Knowledge, Attitudes, Practice , Oral Health , Dental Care/psychology , Mothers/psychology , Pediatrics , Toothbrushing , Interviews as Topic , Decision Making , Qualitative Research , Educational Status , Mother-Child RelationsABSTRACT
In Older Adults (OAs), Electroencephalogram (EEG) slowing in frontal lobes and a diminished muscle atonia during Rapid Eye Movement sleep (REM) have each been effective tracers of Mild Cognitive Impairment (MCI), but this relationship remains to be explored by non-linear analysis. Likewise, data provided by EEG, EMG (Electromyogram) and EOG (Electrooculogram)-the three required sleep indicators-during the transition from REM to Non-REM (NREM) sleep have not been related jointly to MCI. Therefore, the main aim of the study was to explore, with results for Detrended Fluctuation Analysis (DFA) and multichannel DFA (mDFA), the Color of Noise (CN) at the NREM to REM transition in OAs with MCI vs. subjects with good performances. The comparisons for the transition from NREM to REM were made for each group at each cerebral area, taking bilateral derivations to evaluate interhemispheric coupling and anteroposterior and posterior networks. In addition, stationarity analysis was carried out to explore if the three markers distinguished between the groups. Neuropsi and the Mini-Mental State Examination (MMSE) were administered, as well as other geriatric tests. One night polysomnography was applied to 6 OAs with MCI (68.1 ± 3) and to 7 subjects without it (CTRL) (64.5 ± 9), and pre-REM and REM epochs were analyzed for each subject. Lower scores for attention, memory and executive funcions and a greater index of arousals during sleep were found for the MCI group. Results confirmed that EOGs constituted significant markers of MCI, increasing the CN for the MCI group in REM sleep. The CN of the EEG from the pre-REM to REM was higher for the MCI group vs. the opposite for the CTRL group at frontotemporal areas. Frontopolar interhemispheric scaling values also followed this trend as well as right anteroposterior networks. EMG Hurst values for both groups were lower than those for EEG and EOG. Stationarity analyses showed differences between stages in frontal areas and right and left EOGs for both groups. These results may demonstrate the breakdown of fractality of areas especially involved in executive functioning and the way weak stationarity analyses may help to distinguish between sleep stages in OAs.
ABSTRACT
BACKGROUND: Cardiac rehabilitation is a secondary prevention strategy which it includes a set of activities that would assure cardiac patients a place as normal as it could be into the society, being also essential for going back to work, by improving their quality of life and reducing costs for institutions. METHODS: A non-randomized clinical study was conducted at the "Siglo XXI" Cardiology Hospital; We included patients with the diagnosis of ischemic heart disease and/or valve disease, the response variables were: percentage of patients going back to work and disability time upon return to duty. Cardiac rehabilitation program was applied for 1 month and followed up at 2 months and 1 year. RESULTS: Two groups were formed, the ones who received cardiac rehabilitation, N = 40 (experimental group) against a control group, N = 25. The percentage of patients going back to work with a cardiac rehabilitation was 75 % versus 60 % of the group did not receive cardiac rehabilitation, p = 0.2, with a mean of 68 days of disability in the experimental group against 128 in the control group, p = 0.001. CONCLUSIONS: The experimental group showed a higher percentage of patients who returned to work, working time reentry (in days) was lower compared to the control group.
Introducción: en un estudio observacional, retrospectivo evaluamos la frecuencia, presentación clínica y tratamiento de linfocele en pacientes pediátricos con trasplante renal. Métodos: de enero de 2004 a enero de 2009 se realizaron 242 trasplantes renales, 197 de donantes vivos y 45 trasplantes de donantes cadavéricos. La técnica quirúrgica utilizada fue la habitual y la de los implantes uretrales fue la técnica Ricard modificada. El tratamiento fue por punción cutánea y drenajes internos mediante una ventana peritoneal laparoscópica. Resultados: se diagnosticaron siete pacientes con linfoceles (2.9 % con un IC 95 % 0.6-5.2 %). Una paciente del sexo femenino y seis del sexo masculino, todos fueron sometidos a drenajes por punción cutánea, seis pacientes fueron recidivados y tratados exitosamente por drenaje interno mediante una ventana peritoneal laparoscópica. Conclusiones: nuestra frecuencia es igual a la reportada en otros estudios (0.6-18 %). El drenaje interno mediante la ventana peritoneal laparoscópica parece ser lo más apropiado en pacientes pediátricos.
Subject(s)
Cardiac Rehabilitation , Heart Valve Diseases/rehabilitation , Myocardial Ischemia/rehabilitation , Return to Work/statistics & numerical data , Adult , Aged , Female , Follow-Up Studies , Humans , Male , Middle Aged , Prospective Studies , Treatment OutcomeABSTRACT
Multiple biotic and abiotic environmental stress factors affect negatively various aspects of plant growth, development, and crop productivity. Plants, as sessile organisms, have developed, in the course of their evolution, efficient strategies of response to avoid, tolerate, or adapt to different types of stress situations. The diverse stress factors that plants have to face often activate similar cell signaling pathways and cellular responses, such as the production of stress proteins, upregulation of the antioxidant machinery, and accumulation of compatible solutes. Over the last few decades advances in plant physiology, genetics, and molecular biology have greatly improved our understanding of plant responses to abiotic stress conditions. In this paper, recent progresses on systematic analyses of plant responses to stress including genomics, proteomics, metabolomics, and transgenic-based approaches are summarized.
Subject(s)
Biotechnology/methods , Plant Physiological Phenomena , Plants/metabolism , Stress, Physiological , Antioxidants/chemistry , Crops, Agricultural/metabolism , Gene Expression Regulation, Plant , Genomics , Hormones/metabolism , Metabolomics , Proteomics , Signal Transduction , TransgenesABSTRACT
BACKGROUND AND OBJECTIVE: As the periodontal lesion develops, the junctional epithelium migrates apically in conjunction with the dissolution of the most coronal Sharpey's fibers. Because matrix metalloproteinase-9 (MMP-9) has been identified in migrating epithelial cells and invading tumors, we propose that this enzyme is produced by gingival keratinocytes in advanced periodontal lesions. METHODS: To test this idea, biopsies of inflamed gingival tissues were obtained from patients with advanced periodontitis. Healthy gingival tissue samples were utilized as controls. The presence and activity of MMP-9 was evaluated by combining indirect immunofluorescence of gingival tissue samples and gelatin zymography of gingival epithelium separated from connective tissue. RESULTS AND CONCLUSIONS: The staining pattern showed the presence of MMP-9 in junctional and pocket gingival epithelial cells, polymorphonuclear neutrophils (PMNs) and as a scattered deposit along connective tissues of periodontitis-affected gingival tissues. Gelatin zymography permitted the identification of pro-MMP-9 in surcular/pocket epithelium derived from inflamed gingival tissues. Lower levels of MMP-9 were detected in epithelium not exposed to inflammation. These observations suggest a role for MMP-9 in gingival epithelial response to periodontal infection.
