Protective effects of melatonin and glucagon-like peptide-1 receptor agonist (liraglutide) on gastric ischaemia-reperfusion injury in high-fat/sucrose-fed rats.

Ischaemia-reperfusion (I-R) injury is a serious pathology that is often encountered with thrombotic events, during surgery when blood vessels are cross-clamped, and in organs for transplantation. Increased oxidative stress is the main pathology in I-R injury, as assessed in studies on the heart, kidney, and brain with little data available on gastric I-R (GI-R). Liraglutide is a GLP-1 receptor agonist that has insulinotropic and weight reducing actions, and melatonin that has been much studied as a chronotropic hormone; have also studied as being anti-oxidative stress agents. Herein, we aimed to explore the effects of liraglutide and melatonin on GI-R injury with high-fat/sucrose diet. Rats were divided into six groups; two diet-control, two melatonin- and two liraglutide-pretreated groups. All rats were subjected to 30 minutes of gastric ischaemia followed by 1 hour of reperfusion. Gastric tissues were assessed for the percentage of DNA fragmentation, myeloperoxidase activity, total oxidant status, total antioxidant capacity, oxidative stress index, BMI and histopathological examination. We showed that high-fat feeding for four weeks prior to GI-R significantly increased BMI, oxidative stress indices and decreased total antioxidant capacity, with a neutral effect on apoptosis compared to controls. Pretreatment with either melatonin (10 mg/kg per day orally) or liraglutide (25 µg/kg per day ip) reverses these effects. Furthermore, both drugs reduced weight only in HFS-fed rats. Both liraglutide and melatonin have nearly similar protective effects on gastric I-R injury through decreasing the oxidative stress and apoptosis.

Epidemiology, causative agents, and risk factors affecting human otomycosis infections.

BACKGROUND/AIM: Otomycosis, or fungal otitis externa, has typically been described as fungal infection of the external auditory canal, with infrequent complications involving the middle ear. This study assessed a mycological analysis of fungal debris from externalauditory canals of patients at Tanta University Hospital, Gharbia Governorate, Egypt. MATERIALS AND METHODS: The samples were collected over a year from the 110 patients that were clinically diagnosed to have otomycosis. The samples were then inoculated and culture plates were examined for the presence of fungal growth. RESULTS: The results showed that Aspergillus niger was the most common fungus causing otomycosis. It was more common among males aged 21-40 years. The incidence was higher in the summer and spring seasons. Manual workers and students had the highest frequency based on occupational incidence. The most common presenting complaint was itching. Trauma to the external auditory canal was the most common predisposing factor. CONCLUSION: The epidemiological profile of otomycosis infection varied according to different factors in the study population.