ABSTRACT
In this study monoclonal antibodies to oestrogen receptors in the ER-ICA kit (Abbott) and to progesterone receptors (Biogenex) were used to determine the presence of those receptors in fine needle aspirates and cryostat sections. The degree of positivity is semi-quantitated and the results compared to biochemical assays (Dextran-Coated Charcoal Method) performed on the same specimens. Out of 14 cases that were positive on the biochemical assay, 11 (77%) were positive on ER-ICA performed on the FNA material, and there was a 100% agreement on the cryostat sections (11/11). PR-ICA on the cytospin smears and cryostat sections agreed in 63% and 90% respectively.
Subject(s)
Antibodies, Monoclonal , Biopsy, Needle/methods , Breast Neoplasms/chemistry , Breast Neoplasms/pathology , Cryopreservation , Immunohistochemistry/methods , Receptors, Estrogen/analysis , Receptors, Progesterone/analysis , Breast Neoplasms/epidemiology , Evaluation Studies as Topic , Humans , Receptors, Estrogen/immunology , Receptors, Progesterone/immunology , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
During 1990-1991, all patients seen at the surgical out-patient clinic at the Kenyatta National Hospital (KNH) with breast lumps had a fine-needle aspiration biopsy for diagnosis prior to open biopsy. Out of 105 that had corresponding surgical biopsies, 82 aspirates were considered satisfactory. Of these, the correct diagnosis was made in 59 (71%), another 12 (21%) were called benign but the correct histologic diagnosis was not made, 7 (8.5%) were correctly called negatives. The diagnostic accuracy of this method in differentiating benign from malignant was 95% in this study.
