ABSTRACT
Biological assay guided fractionation of a dichloromethane extract of Synaptolepis kirkii led to the isolation of four new and five known daphnane-type diterpene orthoesters, whose structure was established by spectroscopic data. Full spectroscopic data of the new and known natural products are reported here for the first time. Pronounced neurotrophic and substantial antileukaemia activities of these compounds were found in in vitro assays.
Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Nerve Growth Factors/isolation & purification , Animals , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Cell Survival/drug effects , Chick Embryo , Diterpenes/chemistry , Diterpenes/isolation & purification , Diterpenes/pharmacology , Ganglia, Spinal/cytology , Ganglia, Spinal/drug effects , Humans , Leukemia/drug therapy , Models, Molecular , Molecular Structure , Nerve Growth Factors/chemistry , Nerve Growth Factors/pharmacology , Neurons/drug effects , Terpenes/chemistry , Terpenes/isolation & purification , Terpenes/pharmacology , Thymelaeaceae/chemistry , Triterpenes/chemistry , Triterpenes/isolation & purification , Triterpenes/pharmacology , Tumor Cells, CulturedABSTRACT
A new homoprenylated benzochromene, methyl-5,10-dihydroxy-7-methoxy-3-methyl-3-[4-methyl-3-pentenyl]-3H-benzo[f]chromene-9-carboxylate (1), was isolated from the roots of Pentas bussei, collected from Kenya. The structure of this new compound was determined by spectroscopic data interpretation.
Subject(s)
Benzopyrans/isolation & purification , Plants, Medicinal/chemistry , Rubiaceae/chemistry , Benzopyrans/chemistry , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Kenya , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Plant Roots/chemistryABSTRACT
Bioassay-guided fractionation of the dichloromethane extracts of the roots and the bark of Zanthoxylum usambarense led to the isolation of two physiologically active compounds, i.e. canthin-6-one 1 (fungicide) and pellitorine 4 (insecticide). Together with oxychelerythrine 2, norchelerythrine 3, (+)-sesamin 5 and (+)-piperitol-3,3-dimethylallyl ether 6, they were isolated for the first time from this plant.
