ABSTRACT
In the present paper, we report the fabrication of a new sensing membrane for fluorescence detection of metronidazole (MNZ). Briefly, a pyrenebutyric acid derivative, 2-(methacryloyloxy) ethyl-4-(1-pyrenyl) butanoate (MPB) with a double bond, was synthesized and copolymerized with 2-hydroxyethylmethacrylate (HEMA) on the activated glass surface by thermal initiation in the presence of cross-linker. The sensor responds linearly to metronidazole in the concentration range of 1.23~35.48 mg.L(-1) in aqueous solution with a detection limit of 0.36 mg.L(-1). The lifetime is enhanced by covalently immobilizing the pyrenebutyric acid derivative on glass slide, which hinders leaching of the dye from the membrane. The sensor could be regenerated after use by washing in methanol (RSD = 2.42 %), and it shows sufficient stability, and selectivity. Interference of other pharmaceuticals on membrane performance is discussed. The developed membrane has been successfully applied for the direct determination of metronidazole in human serum sample without pretreatment.
