Comparative study of two carnivorous fish (Parupeneus forsskali and Thalassoma klunzingeri) from the Red Sea: Hemato-biochemical parameters and cellular characterization.

Although, the Red sea is highly rich with fish fauna but still the information known is so limited for the researchers especially about the fish physiology baselines. So, in the present study we investigated the heamto-biochemical parameters and cell characterization of two fish having the same feeding habitats. Fish specimens of Red Sea goatfish (Parupeneus forsskali) and Klunzinger's wrasse (Thalassoma klunzingeri) were captured from Hurghada, Egypt. Haematological and biochemical analysis as well as blood cells characterization were performed. The morphological and cytochemical aspects of peripheral blood cells of the two species were studied by light microscopy. Thalassoma klunzingeri showed lower Hct and Hb values and RBCs count. Fusiform to spindle shape thrombocytes were found only in the blood of Parupeneus forsskali while spiked thrombocytes were found only in the blood of Thalassoma klunzingeri. This investigation may be helpful as a tool to monitor the health status of the two species and will be used as biomarkers for clinical pathology.

SDS-PAGE technique as biomarker for fish toxicological studies.

Although many studies on the hematological and biochemical parameters in fishes have been done, still there are some shortage in the estimation and evaluation of the baseline's values of marine and freshwater fishes. Recently, the use of hematology and biochemistry of fishes in toxicology, aquaculture, environmental pollution, feeding, and antioxidants studies has been increased. In this study we introduced the importance of those parameters and their importance as biomarkers in fish toxicology from previous literature and as new findings. Hemato-biochemical parameters were widely used in fish toxicological studies. Many researches have used the protein electrophoresis as a valid tool to determining intra and inter-specific variation among species. Protein profile was extensively used in determining the health of fish, as indicators of anemia or other diseases provide information about the existence of the disease, and in the diagnosis of disease. So, to carry out the aim of this study, we reported one of the more advanced techniques used SDS-PAGE as molecular biomarker for protein profile analysis in fish with shedding the light on the importance of hematological and biochemical parameters in fish toxicological studies.