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FEMS Microbiol Lett ; 125(2-3): 293-7, 1995 Jan 15.
Article in English | MEDLINE | ID: mdl-7875577

ABSTRACT

This paper describes transformation of intact conidia of Aspergillus nidulans, auxotrophic for arginine, by using the biolistic process. The plasmid employed was pFB39, carrying the argB gene. The transformation frequency obtained was 81 transformants/microgram of DNA. Classical genetics and molecular analysis were conducted to analyse transformants and to determine in which chromosome integration took place.


Subject(s)
Aspergillus nidulans/genetics , Genes, Fungal , Plasmids , Transformation, Genetic , Arginine/metabolism , Aspergillus nidulans/physiology , Chromosomes, Fungal , DNA, Fungal/isolation & purification , Gene Transfer Techniques , Genetic Techniques , Karyotyping
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