Bio-separation of value-added products from Kraft lignin: A promising two-stage lignin biorefinery via microbial electrochemical technology.

The most ubiquitous aromatic biopolymer in nature, lignin offers a promising foundation for the development of bio-based chemicals with wide-ranging industrial uses attributable to its aromatic structure. Lignin must first be depolymerized into smaller oligomeric and monomeric units at the initial stage of lignin bioconversion, followed by separation to recover valuable products. This study demonstrates an integrative biorefinery idea based on in-situ depolymerization of the lignin via microbial electro-Fenton reaction in a microbial peroxide-producing cell and recovery of the identified products i.e., phenolic or aromatic monomers by one step high throughput chromatography. The yield percentage of acetovanillone, ethylvanillin, and ferulic acid recovered from the depolymerized lignin using the integrative biorefinery strategy were 2.1 %, 9.1 %, and 9.04 %, respectively. These products have diverse industrial usage and can be employed as platform chemicals. The development of a novel system for efficient simultaneous lignin depolymerization and subsequent quality separation are demonstrated in this study.

Degradation of ß-lactam antibiotic ampicillin using sustainable microbial peroxide producing cell system.

The enormous use of synthetic antibiotic and personal care products has impacted the natural microbiome and ecosystem. Overtime, treatment technologies developed suffered due to incomplete removal hence, a pilot dual-chambered microbial peroxide-producing cell that degrades ampicillin catalyzed by homogenous Fenton-reaction was designed. The system reported maximum current at 16.714 ± 0.048 µAcm-2, power output of 1.956 ± 0.015 mW m-2; 88 ± 2.90 mM of H2O2 generation with Na2SO4 that degraded 95.9 ± 3.00 to 97.8 ± 3.20% of 10 mg L-1ampicillin within 72 hrs with electro-active Shewanella putrefaciens. An E. coli bioactivity assay with ampicillin exhibited no sensitivity zone due to the loss of activity. Analytical spectroscopic studies reveal ß-Lactam ring deformation; Liquid Chromatography-Mass Spectroscopy clearly shows the presence of degradation metabolites. A sustainable wastewater treatment with 72 ± 4.5% reduction in anodic chemical oxygen demand was achieved. Present results designate the technology, as promising for effective antibiotics removal for wastewater treatment concomitant with electricity generation.

Microbial peroxide producing cell mediated lignin valorization.

Lignin is one of the most abundant naturally occurring polymers and can produce value-added products such as vanillin and p-coumaric acid. In the current work, in-situ depolymerization of lignin for its valorization in a microbial peroxide-producing cell (MPPC) system was performed. It is an electrochemical cell that requires no external energy to produce H2O2 for the advanced oxidation process. Lignin in the MPPC system undergoes oxidative depolymerization to generate value-added products. The maximum open-circuit voltage (OCV) was 1.143 V, the current density and power densities were 14 mA/cm2 and 13 mW/cm2, respectively, along with the production of 26 mM of H2O2. The degradation of signature linkages such as ß-ß bond and ß-0-4 bond were analyzed and confirmed using FTIR spectroscopy. Vanillin, p-coumaric acid, ferulic acid, etc. were generated during depolymerization and were detected using LC-QTOF-MS.