Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Nucleic Acids Res ; 26(10): 2481-7, 1998 May 15.
Article in English | MEDLINE | ID: mdl-9580703

ABSTRACT

The atomic force microscope (AFM) was used to assay the extent of DNA condensation in approximately 100 different complexes of DNA with polylysine (PL) or PL covalently attached to the glycoproteins asialoorosomucoid (AsOR) or orosomucoid (OR). The best condensation of DNA was obtained with 10 kDa PL covalently attached to AsOR, at a lysine:nucleotide (Lys:nt) ratio of 5:1 or higher. These conditions produce large numbers of toroids and short rods with contour lengths of 300-400 nm. Some DNA condensation into shortened thickened structures was seen with 10 kDa PL attached to AsOR at Lys:nt ratios of 1.6:1 and 3:1. Some DNA condensation was also seen with 4 kDa PL at Lys:nt ratios of 3:1 and higher. Little DNA condensation was seen with PL alone or with PL convalently attached to OR at Lys:nt ratios up to 6:1. AsOR-PL enhanced gene expression in the mouse liver approximately 10- to 50-fold as compared with PL alone.


Subject(s)
DNA/genetics , DNA/ultrastructure , Gene Transfer Techniques , Microscopy, Atomic Force/methods , Animals , Asialoglycoproteins , Liver/metabolism , Luciferases/genetics , Mice , Mice, Inbred BALB C , Orosomucoid/analogs & derivatives , Plasmids , Polylysine
SELECTION OF CITATIONS
SEARCH DETAIL
...