Factors Affecting Village Apparatus, Integrated Service Post and Early Childhood Education in Stunting Prevention.

Background: The study aimed to identify the achievement of stunting prevention based on the contribution of the Village Apparatus, Integrated Health Services Post and Early Childhood Education. Moreover, it determined the effect of the personnel factors of each agency on the achievement of stunting prevention. Methods: A cross-sectional study was conducted on 175 respondents in Surakarta residences covering Klaten, Boyolali, Karanganyar, Surakarta, Sukoharjo, and Sragen districts from August to October 2021. The researcher conducted a line plot to describe the score of stunting prevention efforts through SINAR APD in planning, funding, implementation, and monitoring. Linear regression and One Way Anova were analyzed using SPSS to determine the effect of the personnel factors. Results: Personnel factors had a significant role in the achievement of funding for Stunting Prevention. The data of stunting showed knowledge (F=3.3; P<0.05), attitudes towards authoritative parenting (F=5.6; P<0.05), and attitudes towards permissive parenting (F=6.1; P<0.05). Conclusions: The main problem was the lack of achievement in the funding aspect. The researcher recommended further research to increase knowledge on stunting for village apparatus, Integrated Health Services Post and Early Childhood Education. Understanding good parenting patterns can change mindsets and attitudes to avoid applying parenting methods that are too authoritarian or permissive.

Relationship Between Gender Equality and Husband Support in the Use of Postpartum Family Planning (PPFP).

Introduction: Postpartum family planning (PPFP) has been reported to decrease the risk of stunting by increasing the interval between pregnancies by 0.9 percent every month. In Indonesia, the prevalence of stunting affects 21.6% in 2022; however, it is expected that by 2024, the figure would drop to 14%. Objective: This study aims to analyze the relationship between gender equality and husband support in the use of PPFP. Methods: The study was conducted using a cross-sectional method and took place from August to October 2022. The participants comprised 210 women who had given birth in the first 4 to 12 months in Kulon Progo, Yogyakarta, Indonesia. The data was collected from women who visited the pediatrics and family planning clinics of community health centers from August to October 2022, using a structured questionnaire and analyzed using both the Chi-Square Test and Binary Logistic Regression Analysis. Results: The results showed that 38.1% of the participants used PPFP. The estimated results reveal that variables such as education, husband support, gender equality, home visits, and postnatal visits (p < 0.05) influenced the implementation of postpartum contraception. While other variables such as age, occupation, income, number of children, and parity did not affect the model (p > 0.05). Conclusion: Participating in postpartum family planning requires the husband's support and gender equality. We recommend a deliberate effort on improving postnatal mothers using postpartum family planning, one of the strategies is to increase intensive outreach to pregnant women with higher education to their husbands about the importance of postpartum family planning.

DeepCov: Effective Prediction Model of COVID-19 Using CNN Algorithm.

COVID-19 outbreak prediction is a challenging and complicated problem in a vast dataset. Several communities have proposed various methods to predict the COVID-19-positive cases. However, conventional techniques remain drawbacks to predicting the actual trend cases. In this experiment, we adopt CNN to build our model by analyzing features from the vast COVID-19 dataset to predict long-term outbreaks to present early prevention. Our model can achieve adequate accuracy with a tiny loss based on the experiment results. In this study, we calculate the function which produces RMSE 0.00070 and MAPE 0.02440 to predict new cases and get RMSE 0.00468 and MAPE 0.06446 for predicting new deaths. Therefore, our proposed method can accurately predict the trend of positive cases in the COVID-19 outbreak.

Users' perceptions of the "My Medicine" mobile app usability.

Introduction: One of the causes of health problems is the use of drugs irrationally, which can result in therapy becoming less effective and inefficient. According to World Health Organization (WHO), more than 50% of the world's drugs are prescribed and used improperly. People still lack information about drugs, how to use drugs and how to get drugs. My Medicine is a drug data information system, how to use drugs, and map locations of pharmacies that sell drugs. This study aims to evaluate the user perception of the use of my medicine. Materials and methods: The method in this study was a quantitative study with a cross-sectional approach. One hundred twenty respondents were included in this study involving communities in Pekalongan district by using univariate analysis. Results: The result was 82% of the respondents explained that the performance or interface user of the My Medicine App was very good and the language was easy to understand, 82% of the respondents said that the information on the My Medicine App is needed daily, 84% of the respondents said that My Medicine App easy to access from the mobile phone, 80% of the respondents explained the data provided in the My Medicine App was accurate and as what they are expected. Conclusion: The conclusion is My Medicine App has a very good performance, the language is easy to understand, the information is needed daily, the app is easy to access through a mobile phone. The data presented in the My Medicine Application is accurate, and by user expectations.

A case study of document readiness in the Administration and Management Working Group on accreditation results at Lalolae Health Center.

OBJECTIVE: The results of the self-assessment assessment at the Lalolae Public Health Center which was the target of the 2017-2019 accreditation assessment in East Kolaka Regency showed that most of the low scores were in the administration and management group. This study aims to determine the relations between the readiness of accreditation documents in the Administration and Management Working Group on the accreditation results of the Lalolae Public Health Center in East Kolaka Regency. METHODS: This type of research is quantitative using a cross sectional design. The number of samples in this study were all people in charge of the administrative and management working group at the Lalolae Public Health Center who were determined by the purposive sampling method. This research was carried out from May 1 to July 20, 2020 and took place at the Lalolae Health Center. The sample in this study is 30 respondents. RESULTS: There are 3 documents in chapter I that have unfulfilled categories, there are 4 documents in chapter ii that have unfulfilled categories, and there are 4 documents in chapter III that have unfulfilled categories. CONCLUSION: There is a relation between the readiness of accreditation documents in the administration and management groups to the results of accreditation of Public Health Center.

Antimutagenic activity of nanoparticles of Rhaphidophora pinnata leaves in mice using micronucleus assay.

Cancer is one of the deadliest diseases in the world. Cancer may occur due to gene mutation. Rhaphidophora pinnata is a plant that has many benefits, especially in the leaves which have been used traditionally to treat cancer. The aim of this research is to test the antimutagenic activity of nanoparticles R. pinnata using the micronucleus method. The mice were induced with cyclophosphamide and then followed with the administration of nanoparticles of R. pinnata at the doses of 50, 100, 200 mg/kg for 7 days. The antimutagenic activity was evaluated at the decrease in the number of micronucleus in 200 polychromatic erythrocytes (PCE) cells of mice bone marrow. The result showed that the reduction of amount of micronucleus in PCE of a negative control group, treatment groups, and normal group is 22.65%, 60.3%, 79.6%, 93.8%, and 100%. These results indicate that the antimutagenic activity of nanoparticle of R. pinnata increases proportionally as the doses were increased. It can be concluded that nanoparticles R. pinnata at the doses of 50, 100, and 200 mg/kg have antimutagenic activity.

Emotional Regulation as a Remedy for Teacher Burnout in Special Schools: Evaluating School Climate, Teacher's Work-Life Balance and Children Behavior.

This research aimed to identify whether improvement in working conditions, children's classroom behavior and work-life balance can lower teacher burnout ratio in Pakistan's special schools by using techniques such as emotions regulation. The researcher employed a quantitative research methodology to fulfill the research's purpose. The data for this research was collected using a questionnaire-based instrument. The confirmatory factor analysis and structural equation modeling techniques were used to test the construct validity and underlying structural relationships. The findings demonstrated that the impacts of all three variables are significant in reducing job burnout in teachers. Emotional regulation helps decrease the impact of working conditions and the children's behavior. Nevertheless, it does not aid work-life balance as it requires other techniques of emotional regulation. The research is significant as it highlights the importance of overall working conditions' improvement for teachers working with special needs children. The improvements are essential because the teachers must take extra effort and emotions into their job compared to a typical teacher. The researcher has highlighted the key finding, implications and limitations of this research besides suggesting directions for future research to facilitate peer researchers.

A case study of document readiness in the Administration and Management Working Group on accreditation results at Lalolae Health Center

Objective: The results of the self-assessment assessment at the Lalolae Public Health Center which was the target of the 2017–2019 accreditation assessment in East Kolaka Regency showed that most of the low scores were in the administration and management group. This study aims to determine the relations between the readiness of accreditation documents in the Administration and Management Working Group on the accreditation results of the Lalolae Public Health Center in East Kolaka Regency. Methods: This type of research is quantitative using a cross sectional design. The number of samples in this study were all people in charge of the administrative and management working group at the Lalolae Public Health Center who were determined by the purposive sampling method. This research was carried out from May 1 to July 20, 2020 and took place at the Lalolae Health Center. The sample in this study is 30 respondents. Results: There are 3 documents in chapter I that have unfulfilled categories, there are 4 documents in chapter ii that have unfulfilled categories, and there are 4 documents in chapter III that have unfulfilled categories. Conclusion: There is a relation between the readiness of accreditation documents in the administration and management groups to the results of accreditation of Public Health Center. (AU)

Xerostomia Therapy Due to Ionized Radiation Using Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells.

OBJECTIVES: The aim of this study was to describe the process of regeneration of damaged salivary glands due to ionizing radiations by bone marrow mesenchymal stem cells (BM-MSCs) transplantation that have been given hypoxic preconditioning with 1% O2 concentration. MATERIALS AND METHODS: Stem cell culture was performed under normoxic (O2: 21%) and hypoxic conditions by incubating the cells for 48 hours in a low oxygen tension chamber consisting of 95% N2, 5% CO2, and 1% O2. Thirty male Wistar rats were divided into four groups: two groups of control and two groups of treatment. A single dose of 15 Gy radiation was provided to the ventral region of the neck in all treatment groups, damaging the salivary glands. BM-MSCs transplantation was performed in the treatment groups for normoxia and hypoxia 24-hour postradiation. STATISTICAL ANALYSIS: Statistical analysis was done using normality test, followed by MANOVA test (p < 0.05). RESULTS: There was a significant difference in the expression of binding SDF1-CXCR4, Bcl-2 (p < 0.05) and also the activity of the enzyme α-amylase in all groups of hypoxia. CONCLUSION: BM-MSCs transplantation with hypoxic precondition increases the expression of binding SDF1-CXCR4, Bcl-2 that contributes to cell migration, cell survival, and cell differentiation.

Hypoxic preconditioning effect on stromal cells derived factor-1 and C-X-C chemokine receptor type 4 expression in Wistar rat's (Rattus norvegicus) bone marrow mesenchymal stem cells (in vitro study).

AIM: To examine the effect of hypoxic preconditions on the ability of bone marrow stem cells culture mediated expression C-X-C chemokine receptor type 4 (CXCR4) and stromal cells derived factor-1 (SDF-1) in vitro. MATERIALS AND METHODS: Bone marrow mesenchymal stem cells (BMSCs) were derived from 12 femurs of 200 g Wistar male rats. The animals were euthanized before BMSCs isolation. BMSCs were divided into two groups, control group: Normoxic condition 21% O2 and treatment group: Hypoxic condition 1% O2. The characterization of BMSCs was analyzed using flow cytometry by cluster differentiation 34 and cluster differentiation 105. The expression of CXCR4 and SDF-1 measured using immunocytochemistry immunofluorescence label after 48-h incubation in a low-tension oxygen chamber with an internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2. All data were subjected to a normality test and then analyzed using t-test statistic (p<0.05). RESULTS: The characterization of bone marrow stem cells showed positive cluster differentiation 34 and cluster differentiation 105. A hypoxic precondition (1% O2) in culture increases CXCR4 (p=0.000) and SDF-1 expression than normoxic conditions (p=0.000) (p<0.05). CONCLUSION: Hypoxic preconditioning with 1% O2 increase CXCR4 and SDF1 expression.

Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids.

The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M. A completely randomized design and the least significant difference test were used in the experimental design, and all measurements were performed in triplicate. The highest yield (29.17%) was obtained from pretreatment with 0.9 M HCl. The gel strength did not differ significantly (p>0.05) according to acid type (280.26-259.62 g Bloom), and the highest viscosity was obtained from the 0.6 M citric acid pretreatment. All the gelatins contained α- and ß-chain components and several degraded peptides (24-66 kDa). The color and Fourier-transform infrared spectrum of the gelatin extracted using 0.9 M HCl were similar to those of commercial bovine skin gelatin. In general, the physicochemical properties of the gelatin complied with the industry standard set by the Gelatin Manufacturers Institute of America, revealing that buffalo hide could serve as a potential alternative source of gelatin.

The thioesterase Bhp is involved in the formation of beta-hydroxytyrosine during balhimycin biosynthesis in Amycolatopsis balhimycina.

The putative hydrolase gene bhp from the balhimycin biosynthetic gene cluster has been cloned and overexpressed in Escherichia coli. The corresponding enzyme Bhp was purified to homogeneity by nickel-chelating chromatography and characterized. Although Bhp has sequence similarities to hydrolases with "haloperoxidase"/perhydrolase activity, it did not show any enzymatic activity with standard "haloperoxidase"/perhydrolase substrates (e.g., monochlorodimedone and phenol red), nonspecific esterase substrates (such as p-nitrophenyl acetate, p-nitrophenyl phosphate and S-thiophenyl acetate) or the model lactonase substrate dihydrocoumarin. However, Bhp could be shown to catalyse the hydrolysis of S-beta-hydroxytyrosyl-N-acetyl cysteamine thioester (beta-OH-Tyr-SNAC) with 15 times the efficiency of S-L-tyrosyl-N-acetyl cysteamine thioester (L-Tyr-SNAC). This is in agreement with the suggestion that Bhp is involved in balhimycin biosynthesis, during which it was supposed to catalyse the hydrolysis of beta-OH-Tyr-S-PCP (PCP=peptidyl carrier protein) to free beta-hydroxytyrosine (beta-OH-Tyr) and strongly suggests that Bhp is a thioesterase with high substrate specificity for PCP-bound beta-OH-Tyr and not a "haloperoxidase"/perhydrolase or nonspecific esterase.