ABSTRACT
OBJECTIVES: The aim of this study was to evaluate the addition of chitosan nanoparticle with concentrations of 0, 10, 20, and 30% to the epoxy resin-based (ERB) sealer on its antibacterial and cytotoxicity effect. METHODS AND MATERIALS: This research was divided into two studies, the first study was the addition of chitosan with a concentration of 0% (as control), 10, 20, and 30% to an ERB sealer on its antibacterial effect, and the second study was on its cytotoxicity. An agar diffusion test was employed to determine the antibacterial effect on Enterococcus faecalis. An MTT (3-{4,5-dimethylthiazol-2-yl}-2,5-diphenyl tetrazolium bromide) assay was utilized to test the cytotoxicity by evaluating cell viability. STATISTICAL ANALYSIS: One-way analysis of variance and Tukey's test (α = 0.05) were used to analyze data obtained from each evaluation with a significance level of 95%. RESULTS: The addition of chitosan nanoparticles at concentrations 10, 20, and 30% produced a greater inhibition zone of E. faecalis (p < 0.05), however, had less cytotoxicity compared with no addition of chitosan (0%) (p < 0.05). CONCLUSION: The addition of chitosan nanoparticles at concentrations 10, 20, and 30% to the ERB sealer produced greater antibacterial and less cytotoxicity compared with no addition of chitosan (0%).
ABSTRACT
Context: Vehicles combined with calcium hydroxide, as an intracanal medicament, play a key factor in affecting antibacterial, calcium release, and pH. Aims: The aim of this study is to investigate the effect of three vehicles (glycerin, chlorhexidine gluconate/CHX, and chitosan nanoparticle) combined with calcium hydroxide as an intracanal medicament on antibacterial efficacy against Enterococcus faecalis, calcium ion release, and pH of at different interval times of 7 and 14 days. Settings and Design: The research was experimental laboratory. Materials and Methods: Each study used 24 samples of eight teeth each and was randomly divided into three groups based on the vehicle of calcium hydroxide: group 1: glycerin, group 2: CHX, and group 3: chitosan nanoparticles. Each vehicle group was then further divided into two subgroups of four teeth based on the interval times (group A: 7 days and group B: 14 days). The antibacterial efficacy was determined using an agar diffusion method. Calcium ion release was analyzed with atomic absorption spectrometry, and pH was measured using a pH meter. Statistical Analysis Used: Data from each study were analyzed by two-way ANOVA, followed by Tukey's test with a significance level of 95%. Results: The results exhibited that chitosan nanoparticles had the highest antibacterial efficacy against E. faecalis, calcium ion release, and pH, while the lowest was glycerin at 7 and 14 days (P < 0.05). Conclusion: Calcium hydroxide combined with chitosan nanoparticle as an intracanal medicament produced the highest antibacterial efficacy against E. faecalis, calcium ion release, and pH than glycerin and CHX at intervals of 7 and 14 days.
ABSTRACT
BACKGROUND: The success of root canal treatment is influenced by hermetic root canal obturation. This study was conducted to analyze the apical sealing ability after the addition of calcite-synthesized hydroxyapatite (HA) as an epoxy resin sealer filler. METHODS: Calcite-synthesized HA powder was prepared using the microwave hydrothermal process. HA resin sealer powder and epoxy resin paste (3:1) were mixed, and concentrations of 10%, 20%, 30%, 40%, and 50% were prepared. A sample of thirty maxillary incisors were prepared in the root canal and then, the crown was cut to leave 13 mm of the root and a working length of 12 mm. The root canal was prepared using the crown-down technique and irrigated using 2.5% sodium hypochlorite and 17% ethylenediaminetetraacetic acid alternately. The samples were divided into six groups, with each group consisting of five roots. Group I was obturated with gutta percha using an epoxy resin sealer without HA (HA-0%) as a control group. In each of the Groups II, III, IV, V, and VI, 10% HA resin sealer, 20% HA, 30% HA, 40% HA, and 50% HA were used. All the samples were incubated in a 10-ml simulated body fluid solution at 37°C for 4 weeks. Apical closure density measurement was done using a scanning electron microscope, and the results were analyzed using the Kruskal-Wallis and Mann-Whitney U-tests. RESULTS: A significant increase in the apical sealing ability was observed in the HA-20% sealer group and the HA-30% and HA-40% groups compared to that in the control group. However, the HA-50% sealer group showed a decrease in the apical sealing ability, whereas the HA-10% sealer group showed no difference. The HA-30% had the highest sealing ability than other concentrations. CONCLUSION: The addition of calcite-synthesized HA as a filler at concentrations of 20%, 30%, and 40% increased the apical sealing ability of the epoxy resin sealer.