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1.
Oncogene ; 29(30): 4317-29, 2010 Jul 29.
Article in English | MEDLINE | ID: mdl-20498628

ABSTRACT

G2/M checkpoint activation after DNA damage results in G2/M cell cycle arrest that allows time for DNA repair before the entry of cells into mitosis. Activation of G2/M checkpoint involves a series of signaling events, which include activation of ataxia telangiectecia-mutated and Rad3-related (ATR) and Chk1 kinases and inhibition of Cdc2/Cyclin B activity. Studies presented in this report show that serine (Ser)/threonine (Thr) protein phosphatase 2A (PP2A) has an important role in G2/M checkpoint activation in response to gamma-irradiation (IR) exposure. Using PP2A inhibitors, as well as siRNA targeting various forms of Ser/Thr protein phosphatases, results presented in this report show that specific PP2A inhibition abrogates IR-induced activation of ATR and Chk1 kinases, as well as phosphorylation of Cdc2-Tyr15, and attenuates IR-induced G2/M arrest. These results suggest an important regulation of PP2A on IR-induced G2/M checkpoint signaling response.


Subject(s)
Cell Division/radiation effects , G2 Phase/radiation effects , Gamma Rays , Protein Phosphatase 2/physiology , Ataxia Telangiectasia Mutated Proteins , Breast Neoplasms/pathology , Cell Cycle Proteins/metabolism , Cell Cycle Proteins/physiology , Cell Line, Tumor , Checkpoint Kinase 1 , DNA-Binding Proteins/metabolism , Enzyme Activation , Female , Humans , Protein Kinases/physiology , Protein Phosphatase 2/antagonists & inhibitors , Protein Serine-Threonine Kinases/metabolism , Protein Serine-Threonine Kinases/physiology , Receptors, Neuropeptide Y/antagonists & inhibitors , Signal Transduction/physiology , Tumor Suppressor Proteins/metabolism
2.
Biochemistry ; 39(37): 11312-8, 2000 Sep 19.
Article in English | MEDLINE | ID: mdl-10985776

ABSTRACT

Both the scaffold (A) and the regulatory (R) subunits of protein phosphatase 2A regulate enzyme activity and specificity. Heterotrimeric enzymes containing different R-subunits differ in their specific activities for substrates. Kinetic parameters for the dephosphorylation of a phosphopeptide by different oligomeric forms of PP2A were determined to begin to elucidate the molecular basis of regulatory subunit effects on phosphatase activity. Using steady state kinetics and the pH dependence of kinetic parameters, we have explored the effect of the A- and R-subunits on the kinetic and chemical mechanism of PP2A. The regulatory subunits affected a broad range of kinetic parameters. The C-subunit and AC dimer were qualitatively similar with respect to the product inhibition patterns and the pH dependence of kinetic parameters. However, a 22-fold decrease in rate and a 4.7-fold decrease in K(m) can be attributed to the presence of the A-subunit. The presence of the R2alpha (Balpha or PR55alpha) subunit caused an additional decrease in K(m) and changed the kinetic mechanism of peptide dephosphorylation. The R2alpha-subunit also caused significant changes in the pH dependence of kinetic parameters as compared to the free C subunit or AC heterodimer. The data support an important role for the regulatory subunits in determining both the affinity of PP2A heterotrimers for peptide substrates and the mechanism by which they are dephosphorylated.


Subject(s)
Phosphoprotein Phosphatases/chemistry , Phosphoprotein Phosphatases/metabolism , Amino Acid Sequence , Animals , Binding, Competitive , Cattle , Chickens , Dimerization , Hydrogen-Ion Concentration , Kinetics , Molecular Sequence Data , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Phosphates/chemistry , Phosphopeptides/chemistry , Phosphopeptides/metabolism , Phosphoprotein Phosphatases/antagonists & inhibitors , Phosphorylation , Protein Phosphatase 2 , Substrate Specificity
3.
Mol Cell Biol ; 20(3): 1021-9, 2000 Feb.
Article in English | MEDLINE | ID: mdl-10629059

ABSTRACT

Initiation of DNA replication in eukaryotes is dependent on the activity of protein phosphatase 2A (PP2A), but specific phosphoprotein substrates pertinent to this requirement have not been identified. A novel regulatory subunit of PP2A, termed PR48, was identified by a yeast two-hybrid screen of a human placental cDNA library, using human Cdc6, an essential component of prereplicative complexes, as bait. PR48 binds specifically to an amino-terminal segment of Cdc6 and forms functional holoenzyme complexes with A and C subunits of PP2A. PR48 localizes to the nucleus of mammalian cells, and its forced overexpression perturbs cell cycle progression, causing a G(1) arrest. These results suggest that dephosphorylation of Cdc6 by PP2A, mediated by a specific interaction with PR48, is a regulatory event controlling initiation of DNA replication in mammalian cells.


Subject(s)
Cell Cycle Proteins/metabolism , DNA Replication , Phosphoprotein Phosphatases/metabolism , Saccharomyces cerevisiae Proteins , Amino Acid Sequence , Animals , Cell Line , Cloning, Molecular , Female , Gene Library , HeLa Cells , Humans , Macromolecular Substances , Mice , Molecular Sequence Data , Phosphoprotein Phosphatases/chemistry , Phosphoprotein Phosphatases/genetics , Phylogeny , Placenta/enzymology , Pregnancy , Protein Phosphatase 2 , Protein Structure, Quaternary , Rabbits , Recombinant Fusion Proteins/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , Sequence Alignment , Sequence Homology, Amino Acid , Spodoptera , Transfection
4.
Brain Res Mol Brain Res ; 73(1-2): 68-77, 1999 Nov 10.
Article in English | MEDLINE | ID: mdl-10581399

ABSTRACT

Protein phosphatase 2A (PP2A) is a ubiquitously expressed serine/threonine phosphatase composed of a heterodimeric core enzyme that associates with a variety of regulatory subunits. A fraction of brain PP2A associates with microtubules and may play a role in regulating phosphorylation of microtubule-associated proteins. We examined the isoform specificity and the mechanism involved in the association of PP2A with brain microtubules. Only the R2alpha (B/PR55alpha) and R2beta (B/PR55beta) regulatory subunits associated with endogenous neural microtubules. Neither the R2gamma (B/PR55gamma) nor members of the R5 (B'/PR56) family of regulatory subunits co-sedimented with microtubules, although abundant amounts of these proteins were detected in brain. The efficient association of PP2A with microtubules in vitro was dependent on an anchoring activity present in a brain protein fraction containing microtubule-associated and microtubule-interacting proteins. Anchoring factor-dependent association of PP2A with microtubules was specific for the heterotrimeric form of PP2A. The core dimer and the isolated subunits of PP2A had very little affinity for microtubules. Characterization of a fraction enriched in the anchoring factor showed that the activity was a heat labile protein that does not correspond to classical microtubule-associated proteins. The anchoring factor associated with microtubules independently of PP2A. These results indicate the association of PP2A with microtubules can be mediated by an anchoring factor that interacts in an isoform-specific manner with heterotrimeric forms of the phosphatase.


Subject(s)
Brain/metabolism , Microtubules/metabolism , Phosphoprotein Phosphatases/metabolism , Animals , Biological Transport , Brain Chemistry , Cattle , Cell Line , Chemical Fractionation , Hot Temperature , Isoenzymes/chemistry , Isoenzymes/metabolism , Microtubule-Associated Proteins/chemistry , Microtubule-Associated Proteins/isolation & purification , Microtubule-Associated Proteins/metabolism , Microtubules/chemistry , Phosphoprotein Phosphatases/chemistry , Protein Binding , Protein Phosphatase 2 , Rats , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Tissue Extracts/chemistry
5.
J Biol Chem ; 274(45): 31917-24, 1999 Nov 05.
Article in English | MEDLINE | ID: mdl-10542219

ABSTRACT

The function of the retinoblastoma protein (pRB) in controlling the G(1) to S transition is regulated by phosphorylation and dephosphorylation on serine and threonine residues. While the roles of cyclin-dependent kinases in phosphorylating and inactivating pRB have been characterized in detail, the roles of protein phosphatases in regulating the G(1)/S transition are not as well understood. We used cell-permeable inhibitors of protein phosphatases 1 and 2A to assess the contributions of these phosphatases in regulating cyclin-dependent kinase activity and pRB phosphorylation. Treating asynchronously growing Balb/c 3T3 cells with PP2A-selective concentrations of either okadaic acid or calyculin A caused a time- and dose-dependent decrease in pRB phosphorylation. Okadaic acid and calyculin A had no effect on pRB phosphatase activity even though PP2A was completely inhibited. The decrease in pRB phosphorylation correlated with inhibitor-induced suppression of G(1) cyclin-dependent kinases including CDK2, CDK4, and CDK6. The inhibitors also caused decreases in the levels of cyclin D2 and cyclin E, and induction of the cyclin-dependent kinase inhibitors p21(Cip1) and p27(Kip1). The decrease in cyclin-dependent kinase activities were not dependent on induction of cyclin-dependent kinase inhibitors since CDK inhibition still occurred in the presence of actinomycin D or cycloheximide. In contrast, selective inhibition of protein phosphatase 1 with tautomycin inhibited pRB phosphatase activity and maintained pRB in a highly phosphorylated state. The results show that protein phosphatase 1 and protein phosphatase 2A, or 2A-like phosphatases, play distinct roles in regulating pRB function. Protein phosphatase 1 is associated with the direct dephosphorylation of pRB while protein phosphatase 2A is involved in pathways regulating G(1) cyclin-dependent kinase activity.


Subject(s)
Cyclin-Dependent Kinases/metabolism , Isoenzymes/metabolism , Phosphoprotein Phosphatases/metabolism , Pyrans , Retinoblastoma Protein/metabolism , Spiro Compounds , 3T3 Cells , Animals , Antifungal Agents/pharmacology , Cyclin G , Cyclin G1 , Cyclins/metabolism , Enzyme Inhibitors/pharmacology , Marine Toxins , Mice , Mice, Inbred BALB C , Okadaic Acid/pharmacology , Oxazoles/pharmacology , Phosphorylation , Protein Phosphatase 1 , Protein Phosphatase 2
6.
Neuron ; 24(2): 481-9, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10571241

ABSTRACT

The large extracellular matrix protein Reelin is produced by Cajal-Retzius neurons in specific regions of the developing brain, where it controls neuronal migration and positioning. Genetic evidence suggests that interpretation of the Reelin signal by migrating neurons involves two neuronal cell surface proteins, the very low density lipoprotein receptor (VLDLR) and the apoE receptor 2 (ApoER2) as well as a cytosolic adaptor protein, Disabled-1 (Dab1). We show that Reelin binds directly and specifically to the ectodomains of VLDLR and ApoER2 in vitro and that blockade of VLDLR and ApoER2 correlates with loss of Reelin-induced tyrosine phosphorylation of Disabled-1 in cultured primary embryonic neurons. Furthermore, mice that lack either Reelin or both VLDLR and ApoER2 exhibit hyperphosphorylation of the microtubule-stabilizing protein tau. Taken together, these findings suggest that Reelin acts via VLDLR and ApoER2 to regulate Disabled-1 tyrosine phosphorylation and microtubule function in neurons.


Subject(s)
Cell Adhesion Molecules, Neuronal/metabolism , Extracellular Matrix Proteins/metabolism , Nerve Tissue Proteins/metabolism , Receptors, LDL/metabolism , Receptors, Lipoprotein/metabolism , Tyrosine/metabolism , tau Proteins/metabolism , Animals , Cell Adhesion Molecules, Neuronal/genetics , Extracellular Matrix Proteins/genetics , Humans , Low Density Lipoprotein Receptor-Related Protein-1 , Mice , Mice, Knockout/genetics , Phosphorylation , Protein Isoforms/metabolism , Receptors, LDL/genetics , Receptors, Lipoprotein/genetics , Reelin Protein , Serine Endopeptidases
7.
J Biol Chem ; 274(36): 25490-8, 1999 Sep 03.
Article in English | MEDLINE | ID: mdl-10464280

ABSTRACT

Hyperphosphorylated forms of the neuronal microtubule (MT)-associated protein tau are major components of Alzheimer's disease paired helical filaments. Previously, we reported that ABalphaC, the dominant brain isoform of protein phosphatase 2A (PP2A), is localized on MTs, binds directly to tau, and is a major tau phosphatase in cells. We now describe direct interactions among tau, PP2A, and MTs at the submolecular level. Using tau deletion mutants, we found that ABalphaC binds a domain on tau that is indistinguishable from its MT-binding domain. ABalphaC binds directly to MTs through a site that encompasses its catalytic subunit and is distinct from its binding site for tau, and ABalphaC and tau bind to different domains on MTs. Specific PP2A isoforms bind to MTs with distinct affinities in vitro, and these interactions differentially inhibit the ability of PP2A to dephosphorylate various substrates, including tau and tubulin. Finally, tubulin assembly decreases PP2A activity in vitro, suggesting that PP2A activity can be modulated by MT dynamics in vivo. Taken together, these findings indicate how structural interactions among ABalphaC, tau, and MTs might control the phosphorylation state of tau. Disruption of these normal interactions could contribute significantly to development of tauopathies such as Alzheimer's disease.


Subject(s)
Microtubules/metabolism , Neurons/metabolism , Phosphoprotein Phosphatases/metabolism , tau Proteins/metabolism , Alzheimer Disease/metabolism , Animals , Cattle , Humans , Neurons/ultrastructure , Phosphorylation , Protein Phosphatase 2
8.
Curr Opin Neurobiol ; 9(3): 336-42, 1999 Jun.
Article in English | MEDLINE | ID: mdl-10395578

ABSTRACT

All of the known protein serine/threonine phosphatases are expressed in the brain. These enzymes participate in a variety of signaling pathways that modulate neuronal activity. The multifunctional activity of many serine/threonine phosphatases is achieved through their association with targeting proteins. Identification and analysis of targeting molecules has led to new insights into the functions of protein phosphatases in neuronal signaling. The recent use of transgenic mice has also increased our understanding of the physiological roles of these enzymes in the brain.


Subject(s)
Brain/enzymology , Calcineurin/metabolism , Neurons/enzymology , Phosphoprotein Phosphatases/metabolism , Saccharomyces cerevisiae Proteins , Signal Transduction/physiology , Animals , Brain/cytology , Protein Phosphatase 2 , Protein Phosphatase 2C
9.
J Biol Chem ; 274(29): 20313-7, 1999 Jul 16.
Article in English | MEDLINE | ID: mdl-10400653

ABSTRACT

The search for potential targets for ceramide action led to the identification of ceramide-activated protein phosphatases, which include protein phosphatase-2A (PP2A) and protein phosphatase-1 (PP1) with roles in regulating apoptosis and cell growth. Thus far, in vitro studies on ceramide-activated protein phosphatases have been restricted to the use of short chain ceramides, limiting the extent of mechanistic insight. In this study, we show that the long chain D-erythro-C18-ceramide activated PP2A (AB'C trimer), PP2Ac (catalytic subunit of PP2A), and PP1gammac and -alphac (catalytic subunits of PP1gamma and -1alpha isoforms, respectively) 2-6-fold in the presence of dodecane, a lipid-solubilizing agent, with 50% maximal activation achieved at approximately 10 microM D-erythro-C18-ceramide. The diastereoisomers of D-erythroC18-ceramide, D-threo-, and L-threo-C18-ceramide, as well as the enantiomeric L-erythro-C18-ceramide, did not activate PP1 or PP2A, but they inhibited PP1 and PP2A activity. The addition of phosphatidic acid decreased the basal activity of PP1c but also increased the stimulation by D-erythro-C18-ceramide from 1.8- to 2. 8-fold and decreased the EC50 of D-erythro-C18-ceramide to 4.45 microM. The addition of 150 mM KCl decreased the basal activity of PP1 and the dose of D-erythro-C18-ceramide necessary to activate PP1c (EC50 = 6.25 microM) and increased the ceramide responsiveness up to 10-17-fold. These studies disclose stereospecific activation of PP1 and PP2A by long chain natural ceramides under near physiologic ionic strengths in vitro. The implications of these studies for mechanisms of ceramide action are discussed.


Subject(s)
Ceramides/pharmacology , Phosphatidic Acids/pharmacology , Phosphoprotein Phosphatases/metabolism , Cations , Enzyme Activation , Osmolar Concentration , Protein Phosphatase 1 , Protein Phosphatase 2
10.
J Biol Chem ; 273(52): 35339-46, 1998 Dec 25.
Article in English | MEDLINE | ID: mdl-9857076

ABSTRACT

SV40 small tumor antigen (small-t) was used as a model to identify structural elements involved in the interactions between regulatory proteins and protein phosphatase 2A (PP2A). Using mutant proteins and synthetic peptides, we identified a small domain within small-t that is a major site for interaction with the dimeric form of PP2A. A series of small-t truncation mutants identified a region surrounding the first of two conserved cysteine clusters that was critical for interaction with PP2A. These mutants also identified additional regions of small-t that contribute to high affinity interaction. Deletion of residues 110-119, which encompass the first cysteine cluster, resulted in a protein that failed to bind to PP2A. Synthetic peptides that contained residues 105-122 of small-t blocked binding of small-t to PP2A. These peptides also inhibited the phosphatase activity of PP2A in a manner analogous to full-length small-t. The active small-t peptides adopt a beta-strand structure that was essential for high affinity interaction with the PP2A dimer. Based on circular dichroism measurements, the same cysteine cluster-containing peptides that bind to PP2A also interact with zinc. Interaction with zinc required the conserved cysteines but was not required for interaction with PP2A.


Subject(s)
Antigens, Polyomavirus Transforming/metabolism , Phosphoprotein Phosphatases/metabolism , Simian virus 40/immunology , Amino Acid Sequence , Antigens, Polyomavirus Transforming/genetics , Binding Sites , Circular Dichroism , Molecular Sequence Data , Mutation , Peptide Fragments/drug effects , Peptide Fragments/metabolism , Phosphoprotein Phosphatases/antagonists & inhibitors , Protein Binding , Protein Conformation , Protein Phosphatase 2 , Sequence Homology, Amino Acid , Zinc/pharmacology
11.
Mol Cell Biol ; 18(11): 6595-604, 1998 Nov.
Article in English | MEDLINE | ID: mdl-9774674

ABSTRACT

We have previously shown that a WD-40 repeat protein, TRIP-1, associates with the type II transforming growth factor beta (TGF-beta) receptor. In this report, we show that another WD-40 repeat protein, the Balpha subunit of protein phosphatase 2A, associates with the cytoplasmic domain of type I TGF-beta receptors. This association depends on the kinase activity of the type I receptor, is increased by coexpression of the type II receptor, which is known to phosphorylate and activate the type I receptor, and allows the type I receptor to phosphorylate Balpha. Furthermore, Balpha enhances the growth inhibition activity of TGF-beta in a receptor-dependent manner. Because Balpha has been characterized as a regulator of phosphatase 2A activity, our observations suggest possible functional interactions between the TGF-beta receptor complex and the regulation of protein phosphatase 2A.


Subject(s)
Activin Receptors, Type I , Phosphoprotein Phosphatases/chemistry , Protein Serine-Threonine Kinases/metabolism , Receptors, Transforming Growth Factor beta/metabolism , 3T3 Cells , Animals , Cell Division/physiology , Cross-Linking Reagents/metabolism , Cyclin A/metabolism , Gene Expression Regulation, Enzymologic/genetics , Genes, Reporter , Membrane Proteins/metabolism , Mice , Phosphorylation , Protein Kinases/metabolism , Protein Phosphatase 2 , Receptor, Transforming Growth Factor-beta Type I , Receptor, Transforming Growth Factor-beta Type II , Recombinant Fusion Proteins/metabolism , Signal Transduction/physiology
12.
J Cell Biol ; 142(6): 1399-411, 1998 Sep 21.
Article in English | MEDLINE | ID: mdl-9744873

ABSTRACT

The regulated sorting of proteins within the trans-Golgi network (TGN)/endosomal system is a key determinant of their biological activity in vivo. For example, the endoprotease furin activates of a wide range of proproteins in multiple compartments within the TGN/endosomal system. Phosphorylation of its cytosolic domain by casein kinase II (CKII) promotes the localization of furin to the TGN and early endosomes whereas dephosphorylation is required for efficient transport between these compartments (Jones, B.G., L. Thomas, S.S. Molloy, C.D. Thulin, M.D. Fry, K.A. Walsh, and G. Thomas. 1995. EMBO [Eur. Mol. Biol. Organ.] J. 14:5869-5883). Here we show that phosphorylated furin molecules internalized from the cell surface are retained in a local cycling loop between early endosomes and the plasma membrane. This cycling loop requires the phosphorylation state-dependent furin-sorting protein PACS-1, and mirrors the trafficking pathway described recently for the TGN localization of furin (Wan, L., S.S. Molloy, L. Thomas, G. Liu, Y. Xiang, S.L. Ryback, and G. Thomas. 1998. Cell. 94:205-216). We also demonstrate a novel role for protein phosphatase 2A (PP2A) in regulating protein localization in the TGN/endosomal system. Using baculovirus recombinants expressing individual PP2A subunits, we show that the dephosphorylation of furin in vitro requires heterotrimeric phosphatase containing B family regulatory subunits. The importance of this PP2A isoform in directing the routing of furin from early endosomes to the TGN was established using SV-40 small t antigen as a diagnostic tool in vivo. The role of both CKII and PP2A in controlling multiple sorting steps in the TGN/endosomal system indicates that the distribution of itinerant membrane proteins may be acutely regulated via signal transduction pathways.


Subject(s)
Carrier Proteins , Endosomes/metabolism , Isoenzymes/metabolism , Phosphoprotein Phosphatases/metabolism , Subtilisins/metabolism , Amino Acid Sequence , Binding Sites , Biological Transport , Capsid , Furin , Golgi Apparatus , HeLa Cells , Humans , Molecular Sequence Data , Phosphorylation , Protein Phosphatase 2 , Subtilisins/genetics , Vesicular Transport Proteins
13.
Biochemistry ; 37(32): 11232-8, 1998 Aug 11.
Article in English | MEDLINE | ID: mdl-9698369

ABSTRACT

Ceramide has emerged as a potential regulator of diverse cellular functions, and a few direct targets have been identified for its action including protein kinases and phosphatases. In this study, we have purified the predominant ceramide-activated protein phosphatase (CAPP) from rat brain. Utilizing a novel chromatographic approach, CAPP was purified to near homogeneity using hydrophobic interaction chromatography on phenyl Sepharose followed by anion-exchange chromatography on MonoQ. The purified protein was composed of three major bands on SDS-polyacrylamide gel electrophoresis which comigrated with the three subunits of heterotrimeric PP2A. Immunologic studies further identified CAPP to be composed predominantly of heterotrimeric AB'C and ABalphaC as well as heterodimeric PP2A (AC), where C is the catalytic subunit, and A and B are regulatory subunits. These results were also supported by the coelution of CAPP with trimeric and dimeric PP2A on size-exclusion chromatography. These studies provide a convenient and efficient method for the isolation of trimeric and dimeric PP2A, and they allow the biochemical investigation of CAPP.


Subject(s)
Ceramides/physiology , Phosphoprotein Phosphatases/chemistry , Phosphoprotein Phosphatases/isolation & purification , Animals , Blotting, Western , Brain , Chromatography, Agarose , Chromatography, Gel , Chromatography, Ion Exchange , Electrophoresis, Polyacrylamide Gel , Enzyme Activation/drug effects , Phosphoprotein Phosphatases/drug effects , Rats , Sepharose/analogs & derivatives , Sphingosine/analogs & derivatives , Sphingosine/pharmacology
14.
J Biol Chem ; 272(24): 15220-6, 1997 Jun 13.
Article in English | MEDLINE | ID: mdl-9182545

ABSTRACT

Inhibitors of type 1 and type 2A protein phosphatases were used to examine the involvement of protein phosphorylation in regulating the functions of endogenous p53. Exposure of Balb/c 3T3 cells to okadaic acid, an inhibitor of protein phosphatases 1 and 2A, increased the phosphorylation of p53 without changing p53 levels. Okadaic acid treatment enhanced the binding of p53 to a consensus DNA target sequence and caused a 5-8-fold increase in p53 transcriptional activity. Transient expression of SV40 small tumor antigen, a specific inhibitor of protein phosphatase 2A, caused a 4-fold increase in p53 transcriptional activity. Incubation of Balb/c 3T3 cells with okadaic acid also induced programmed cell death in a dose- and time-dependent manner. Decreases in viability, morphological changes, and the appearance of DNA fragmentation were dependent on p53 since cells lacking functional p53 were resistant to okadaic acid-induced apoptosis. The p53-dependent apoptosis induced by okadaic acid was rapid and did not require p53 transcriptional activity. The fact that SV40 small tumor antigen did not induce apoptosis provides additional evidence that p53 transcriptional activity is not sufficient for p53-mediated apoptosis. These results indicate that signaling pathways involving protein phosphorylation play critical roles in controlling the apoptotic activity of p53. Furthermore, a basal level of protein phosphatase 1 or 2A activity is necessary to prevent p53-dependent apoptosis.


Subject(s)
Apoptosis/genetics , Phosphoprotein Phosphatases/antagonists & inhibitors , Transcriptional Activation , Tumor Suppressor Protein p53/metabolism , 3T3 Cells , Animals , Antigens, Polyomavirus Transforming/metabolism , Apoptosis/drug effects , DNA Fragmentation/drug effects , Enzyme Inhibitors/pharmacology , Mice , Mice, Inbred BALB C , Okadaic Acid/pharmacology , Phosphorylation , Protein Phosphatase 1 , Protein Phosphatase 2 , Simian virus 40/immunology , Transcription, Genetic , Tumor Suppressor Protein p53/genetics
15.
Arch Biochem Biophys ; 339(1): 210-7, 1997 Mar 01.
Article in English | MEDLINE | ID: mdl-9056251

ABSTRACT

Alterations in protein phosphatase 2A (PP2A) during retinoic acid-induced differentiation of HL-60 cells have been investigated. PP2A activity of HL-60 cells for phosphorylated myelin basic protein showed a sharp and transient increase after 18-h treatment with 1 microM retinoic acid, which corresponded to G1/S boundary of the cell cycle. This PP2A of the 18-h treated cells was eluted from a DEAE-Sepharose column with 0.13 M NaCl, while PP2A from control cells was eluted with 0.23 M NaCl. The phosphorylase phosphatase activity of PP2A in the 0.13 M eluate was greatly enhanced in the presence of protamine compared with that of the later eluting PP2A. Immunoblot analyses with antisera against B' and B alpha subunits showed that the PP2A in the 0.13 M NaCl eluate from 18-h retinoic acid-treated cells was PP2A0 (AC-B'), whereas the PP2A eluted with 0.23 M NaCl from 24-h retinoic acid-treated cells and 0-, 18-, and 24-h control cells was PP2A1 (AC-B alpha). These results strongly suggest that PP2A undergoes a transient and reversible interconversion of holoenzyme forms during the initial stage of retinoic acid-induced granulocytic differentiation. PP2A activity assayed after dissociation of the catalytic subunit, for phosphorylase as substrate, showed a sharp and transient decrease in S phase of HL-60 cells irrespective of the presence or absence of retinoic acid. Immunoblot analyses with antisera against C-terminus and N-terminus of the catalytic subunit of PP2A suggested that a modification at the C-terminus is responsible for the decrease in PP2A activity. Immunoreactivity to the C-terminal antibody was restored after treatments of the S-phase extract with alkali or ethanol, the conditions which remove the methyl group from the C-terminus. These results suggest that the C-terminus of PP2A catalytic subunit is transiently methylated in S phase of HL-60 cells.


Subject(s)
Granulocytes/cytology , HL-60 Cells/enzymology , Phosphoprotein Phosphatases/metabolism , S Phase , Tretinoin/pharmacology , Blotting, Western , Cell Differentiation/drug effects , Cell Division , HL-60 Cells/cytology , Humans , Macromolecular Substances , Methylation , Phosphorylation , Protein Phosphatase 2
16.
Neuron ; 17(6): 1201-7, 1996 Dec.
Article in English | MEDLINE | ID: mdl-8982166

ABSTRACT

Recently, we reported that a pool of protein phosphatase 2A (PP2A) is associated with microtubules. Here, we demonstrate that specific isoforms of PP2A bind and dephosphorylate the neuronal microtubule-associated protein tau. Coexpression of tau and SV40 small t, a specific inhibitor of PP2A, in CV-1, NIH 3T3, or NT2 cells induced the phosphorylation of tau at multiple sites, including Ser-199, Ser-202, Thr-205, Ser-396, and Ser-404. Immunofluorescent and biochemical analyses revealed that hyperphosphorylation correlated with dissociation of tau from microtubules and a loss of tau-induced microtubule stabilization. Taken together, these results support the hypothesis that PP2A controls the phosphorylation state of tau in vivo.


Subject(s)
Microtubules/metabolism , Phosphoprotein Phosphatases/metabolism , tau Proteins/metabolism , 3T3 Cells , Animals , Cell Line , Drug Stability , Epitopes , Isoenzymes/metabolism , Mice , Phosphorylation , Protein Phosphatase 2 , tau Proteins/chemistry , tau Proteins/immunology
17.
Mol Cell Biol ; 16(11): 6593-602, 1996 Nov.
Article in English | MEDLINE | ID: mdl-8887688

ABSTRACT

We and others previously showed that cyclin G is a transcriptional target of the p53 tumor suppressor protein. However, cellular proteins which might form a complex with cyclin G have not yet been identified. To gain insight into the biological role of cyclin G, we used the yeast two-hybrid screen and isolated two mouse cDNAs encoding cyclin G-interacting proteins. Interestingly, both positive cDNAs encoded B' regulatory subunits of protein phosphatase 2A (PP2A). One clone encodes B'alpha, while the other clone codes for a new member of the B' family, B'beta. B'beta is 70% identical to other members of the B' family. B'alpha associated both in vitro and in vivo with cyclin G but not with the other mammalian cyclins. Furthermore, cyclin G formed a complex with B'alpha only after induction of p53 in p53 temperature-sensitive cell lines. These results indicate that cyclin G forms a specific complex with the B' subunit of PP2A and that complex formation is regulated by p53. Potential roles for the cyclin G-B' complex in p53-mediated pathways are discussed.


Subject(s)
Cyclins/metabolism , Protein Tyrosine Phosphatases/metabolism , Tumor Suppressor Protein p53/metabolism , Alternative Splicing , Amino Acid Sequence , Animals , Baculoviridae , COS Cells , Cell Line , Cloning, Molecular , Cyclin G , Cyclin G1 , Cyclins/chemistry , Macromolecular Substances , Mammals , Mice , Molecular Sequence Data , Open Reading Frames , Protein Phosphatase 2 , Protein Tyrosine Phosphatases/chemistry , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/growth & development , Sequence Homology, Amino Acid , Spodoptera , Transfection
18.
J Biol Chem ; 271(9): 5164-70, 1996 Mar 01.
Article in English | MEDLINE | ID: mdl-8617797

ABSTRACT

Differential association of regulatory B subunits with a core heterodimer, composed of a catalytic (C) and a structural (A) subunit, is an important mechanism that regulates protein phosphatase 2A (PP2A). We have isolated and characterized three novel cDNAs related to the B' subunit of bovine cardiac PP2A. Two human (B'alpha1 and B'alpha2) and a mouse (B'alpha3) cDNA encode for alternatively spliced variants of the B subunit. The deduced primary sequences of these clones contain 12 of 15 peptides derived from the purified bovine B' subunit. Differences between the deduced sequences of the B alpha splice variants and the cardiac peptide sequences suggest the existence of multiple isoforms of the B' subunit. Comparison of the protein and nucleotide sequences of the cloned cDNAs show that all three forms of B'alpha diverge at a common splice site near the 3'-end of the coding regions. Northern blot and reverse transcription-polymerase chain reaction analyses revealed that the B'alpha transcripts (4.3-4.4 kb) are widely expressed and very abundant in heart and skeletal muscle. The expressed human and mouse B'alpha proteins readily associated with the PP2A core enzyme in both in vitro and in vivo complex formation assays. Immunofluorescence microscopy revealed that epitope-tagged B'alpha was localized in both the cytosol and nuclei of transiently transfected cells. The efficiency of binding of all three expressed proteins to a glutathione S-transferase-A subunit fusion protein was greatly enhanced by the addition of the C subunit. Expression of the B'alpha subunits in insect Sf9 cells resulted in formation of AC.B'alpha heterotrimers with the endogenous insect A and C subunits. These results show that the B' subunit, which is the predominant regulatory subunit in cardiac PP2A, is a novel protein whose sequence is unrelated to other PP2A regulatory subunits. The nuclear localization of expressed B'alpha suggests that some variants of the B' subunit are involved in the nuclear functions of PP2A.


Subject(s)
Gene Expression , Muscle, Skeletal/enzymology , Myocardium/enzymology , Phosphoprotein Phosphatases/biosynthesis , 3T3 Cells , Amino Acid Sequence , Animals , Base Sequence , Cattle , Cloning, Molecular , DNA Primers , DNA, Complementary , Gene Library , Glutathione Transferase/biosynthesis , Humans , Macromolecular Substances , Mice , Molecular Sequence Data , Organ Specificity , Phosphoprotein Phosphatases/chemistry , Polymerase Chain Reaction , Protein Multimerization , Protein Phosphatase 2 , Recombinant Fusion Proteins/biosynthesis , Sequence Homology, Amino Acid , T-Lymphocytes/enzymology , Transcription, Genetic , Transfection
19.
J Cell Biol ; 128(6): 1131-44, 1995 Mar.
Article in English | MEDLINE | ID: mdl-7896877

ABSTRACT

Immunofluorescence microscopy revealed the presence of protein phosphatase 2A (PP2A) on microtubules in neuronal and nonneuronal cells. Interphase and mitotic spindle microtubules, as well as centrosomes, were all labeled with antibodies against individual PP2A subunits, showing that the AB alpha C holoenzyme is associated with microtubules. Biochemical analysis showed that PP2A could be reversibly bound to microtubules in vitro and that approximately 75% of the PP2A in cytosolic extracts could interact with microtubules. The activity of microtubule-associated PP2A was differentially regulated during the cell cycle. Enzymatic activity was high during S phase and intermediate during G1, while the activity in G2 and M was 20-fold lower than during S phase. The amount of microtubule-bound PP2A remained constant throughout the cell cycle, implying that cell cycle regulation of its enzymatic activity involves factors other than microtubules. These results raise the possibility that PP2A regulates cell cycle-dependent microtubule functions, such as karyokinesis and membrane transport.


Subject(s)
Microtubules/metabolism , Phosphoprotein Phosphatases/metabolism , Animals , Cattle , Cell Cycle , Cells, Cultured , Fluorescent Antibody Technique , Haplorhini , Mice , Protein Binding , Protein Phosphatase 2 , Rats
20.
Cancer Res ; 55(4): 774-80, 1995 Feb 15.
Article in English | MEDLINE | ID: mdl-7850788

ABSTRACT

To elucidate the roles of protein phosphatases type 1 (PP1) and type 2A (PP2A) in 1,25-dihydroxy-cholecalciferol [1,25(OH)2D3]-induced differentiation of HL-60 cells into monocytes, we examined the enzyme activity and the protein and gene expressions of PP1 and PP2A in these cells. Calyculin-A augmented the 1,25(OH)2D3-induced differentiation of the cells. Treatment of the cells with 1,25(OH)2D3 led to a decrease in PP1-like activity in the cytosol fraction, with a concomitant increase in the membrane and nuclear PP1-like activity, as determined when protein phosphatase activity was assayed using myosin light chain as substrate in the presence of 5 nM okadaic acid. Western blot analysis with antibodies specific for PP1 catalytic subunit isozymes (PP1 alpha, PP1 gamma, and PP1 delta) showed that all three PP1 isozymes were expressed but were differentially distributed in each cellular fraction. Subcellular redistribution of PP1-like activity during 1,25(OH)2D3-induced differentiation was mainly attributed to PP1 gamma and PP1 alpha proteins. In contrast, the localizations of PP1 delta and PP2A catalytic and regulatory subunits were not significantly affected by 1,25(OH)2D3 treatment. The gene expressions of PP1 alpha and PP1 gamma appeared to be constant during processes of monocytic differentiation. The correlation between phenotypic and functional changes of HL-60 cells on the one hand and subcellular redistribution of PP1-like activity on the other suggest that the translocations of PP1 alpha and PP1 gamma isozymes may contribute to the 1,25(OH)2D3-induced monocytic differentiation of HL-60 cells.


Subject(s)
Calcitriol/pharmacology , Isoenzymes/genetics , Leukemia, Promyelocytic, Acute/enzymology , Monocytes/drug effects , Monocytes/enzymology , Phosphoprotein Phosphatases/genetics , Translocation, Genetic , Catalysis , Cell Differentiation/drug effects , Humans , Isoenzymes/metabolism , Leukemia, Promyelocytic, Acute/drug therapy , Leukemia, Promyelocytic, Acute/pathology , Macromolecular Substances , Marine Toxins , Monocytes/cytology , Oxazoles/pharmacology , Phosphoprotein Phosphatases/antagonists & inhibitors , Phosphoprotein Phosphatases/metabolism , Protein Phosphatase 1 , RNA, Messenger/genetics
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