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Biotechnol Prog ; 20(1): 393-6, 2004.
Article in English | MEDLINE | ID: mdl-14763869

ABSTRACT

Magnetic effects induced in ethanolic fermentation by Saccharomyces cerevisiae strain DAUFPE-1012 were studied during a 24 h exposure to 220 mT steady magnetic fields (SMF) at 23 +/- 1 degrees C, produced by NdFeB rod magnets. The magnets were attached diametrically opposed (N to S) to a cylindrical tube reactor. The biomass growth in the reactor culture media (yeast extract + glucose 2%) during 24 h was monitored by measurements of optical density, which was correlated to cell dry weight. Ethanol concentration and glucose level were measured every 2 h. The pH of the culture media was maintained between 4 and 5. As a result, biomass (g/L) increased 2.5-fold and ethanol concentration 3.4-fold in magnetized cultures (n = 8) as compared with SMF nonexposed cultures (n = 8). Glucose consumption was higher in magnetized cultures, which correlated to the ethanol yield.


Subject(s)
Bioreactors/microbiology , Cell Culture Techniques/methods , Electromagnetic Fields , Ethanol/metabolism , Glucose/metabolism , Saccharomyces cerevisiae/physiology , Saccharomyces cerevisiae/radiation effects , Cell Division/physiology , Cell Division/radiation effects , Fermentation/physiology , Fermentation/radiation effects , Radiation Dosage , Saccharomyces cerevisiae/cytology
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