ABSTRACT
1. Ecdysteroids were detected in extracts of egg-producing adult Caenorhabditis elegans, in dauer larvae and in dietary bacteria. 2. Similar concentrations of free ecdysteroids were recorded in adults and larvae, although the two life cycle stages differed in their ratio of ecdysone: 20-hydroxyecdysone. 3. Patent adults metabolized [3H]ecdysone into apolar products and putative [3H]ecdysone 22-phosphate.
Subject(s)
Caenorhabditis/growth & development , Insect Hormones/metabolism , Animals , Biotransformation , Caenorhabditis/metabolism , Ecdysone/metabolism , LarvaABSTRACT
Both patent and prepatent adult Hymenolepis diminuta excreted 20-hydroxyecdysone into the culture medium when maintained in vitro. Patent worms also excreted ecdysone and comparatively large quantities of unidentified immunoreactive material of a relatively apolar nature. This latter material was shown to be depleted from the endogenous free ecdysteroids of patent adults during the culture period. Ecdysteroid excretion was affected both qualitatively and quantitatively when culturing conditions were varied.
Subject(s)
Ecdysterone/metabolism , Hymenolepis/metabolism , Animals , Chromatography, High Pressure Liquid , Ecdysone/metabolism , Gas Chromatography-Mass Spectrometry , In Vitro Techniques , Male , Radioimmunoassay , Rats , Rats, Inbred StrainsABSTRACT
Prepatent and patent adult Hymenolepis diminuta from the intestines of rats, H. diminuta eggs recovered from the faeces of rats harbouring patent infections, and infective cysticercoids from the beetle intermediate host were analysed for free and conjugated ecdysteroids. Adult worms and eggs contained both free ecdysteroids and hydrolysable polar conjugated ecdysteroids, with comparatively large amounts of immunoreactive material also being detected following hydrolysis of the possible apolar conjugated ecdysteroid fraction. Free ecdysteroids were not detected in the cysticercoid sample. The concentration of free ecdysteroids in H. diminuta eggs was higher than that detected in the tissues of the adult worms. Ecdysone and 20-hydroxyecdysone were the major identified compounds of the free ecdysteroid fraction, whereas in the hydrolysed polar conjugated ecdysteroid fraction these two compounds were accompanied by 20,26-dihydroxyecdysone. The free ecdysteroid fraction also contained comparatively large amounts of unidentified immunoreactive material.
Subject(s)
Hymenolepis/analysis , Invertebrate Hormones/analysis , Animals , Chromatography, High Pressure Liquid , Ecdysone/analysis , Ecdysteroids , Ecdysterone/analysis , Gas Chromatography-Mass Spectrometry , Hymenolepiasis/parasitology , Hymenolepis/growth & development , Invertebrate Hormones/isolation & purification , Male , Radioimmunoassay , Rats , Rats, Inbred Strains , TenebrioABSTRACT
Both free ecdysteroids and hydrolysable polar conjugated ecdysteroids were detected in protoscoleces of Echinococcus granulosus from the equine host, and in hydatid cyst fluid from the same source. Comparisons were made of hydatid cyst fluid from E. granulosus infections of three intermediate host species: horses, sheep and humans. Ecdysone and 20-hydroxyecdysone were identified in both protoscoleces and hydatid cyst fluids by high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by capillary gas chromatography/mass spectrometry (selected ion monitoring). The free ecdysteroid fractions of hydatid cyst fluid from horses and sheep also contained several unidentified, chromatographically unique, immunoreactive compounds which were refractory to hydrolysis with a crude Helix pomatia aryl sulphatase enzyme preparation.
