Anaerobic biodegradation of disposable PLA-based products: Assessing the correlation with physical, chemical and microstructural properties.

In the present study commercial Polylactic Acid-based disposable cups and plates were selected for lab scale anaerobic degradability tests. The experiments were carried out under thermophilic conditions at different inoculum to substrate ratios and test material sizes, and the specific biogas production and associated kinetics were evaluated. Maximum biogas production was comparable for almost all the experimental runs (1620 and 1830 NmL/gTOCPLA) and a biodegradation degree in the range 86-100% was attained. Moreover, physical, chemical and microscopical analyses were used to characterize the tested materials before and after the degradation. The products composition was assessed and the presence of some additives (mainly Ca-based) was detected. Potential correlations among the process parameters and product composition were derived and a delay in process kinetics with increasing amount of additives embedded in the polymeric matrix was observed, confirming the relevant influence of the chemical blend on the biodegradation process.

Bridging biotechnology and nanomedicine to produce biogreen whey-nanovesicles for intestinal health promotion.

New intestinal health-promoting biotechnological nanovesicles were manufactured by combining the main environmental pollutant generated from the cheese-making process, whey, with phospholipid, sodium hyaluronate and dextrin, thus overcoming environmental and medical challenges. An efficient, consolidated and eco-friendly preparation method was employed to manufacture the vesicles and the bioactive whey was obtained by mesophilic dark fermentation without external inoculum through a homolactic pathway, which was operated in such a way as to maximize the production of lactic acid. The biotechnological nutriosomes and hyalonutriosomes were relatively small (∼100 nm) and characterized by the net negative surface charge (>-30 mV). The addition of maltodextrin to the liposomes and especially to the hyalurosomes significantly stabilized the vesicles under acidic conditions, simulating the gastric environment, as their size and polydispersity index were significantly lower (p < 0.05) than those of the other formulations. The vesicles were effectively internalized by Caco-2 cells and protected them against oxidative stress. Nutriosomes promoted the proliferation of Streptococcus salivarius, a human commensal bacterium, to a better extent (p < 0.05) than liposomes and hyalurosomes, as a function of the concentration tested. These findings could open a new horizon in intestinal protection and health promotion by integrating biotechnology, nanomedicine, sustainability principles and bio-circular economy.

Dark fermentative volatile fatty acids production from food waste: A review of the potential central role in waste biorefineries.

Volatile fatty acids (VFAs) are high-value chemicals that are increasingly demanded worldwide. Biological production via food waste (FW) dark fermentation (DF) is a promising option to achieve the sustainability and environmental benefits typical of biobased chemicals and concurrently manage large amounts of residues. DF has a great potential to play a central role in waste biorefineries due to its ability to hydrolyze and convert complex organic substrates into VFAs that can be used as building blocks for bioproducts, chemicals and fuels. Several challenges must be faced for full-scale implementation, including process optimization to achieve high and stable yields, the development of efficient techniques for selective recovery and the cost-effectiveness of the whole process. This review aims to critically discuss and statistically analyze the existing relationships between process performance and the main variables of concern. Moreover, opportunities, current challenges and perspectives of a FW-based and fermentation-centred biorefinery layout are discussed.

A cascade biorefinery for grape marc: Recovery of materials and energy through thermochemical and biochemical processes.

The agro-industrial sector makes a high contribution to greenhouse gas emissions; therefore, proper waste management is crucial to reduce the carbon footprint of the food chain. Hydrothermal carbonization (HTC) is a promising and flexible thermochemical process for converting organic materials into energy and added-value products that can be used in different applications. In this work, grape marc residues before and after an extraction process for recovering polyphenols were hydrothermally treated at 220 °C for 1 h. The resulting hydrochar and process water were investigated to test an innovative cascade approach aimed at a multiple product and energy recovery based on the integration of HTC with anaerobic digestion. The results show that this biorefinery approach applied to grape marc could allow to diversify and integrate its potential valorisation options. The produced hydrochars possess an increased fixed carbon content compared to the feedstock (up to +70 %) and, therefore, can be used in soil, immobilizing carbon in a stable form and partially replacing peat in growing media (up to 5 % in case of hydrochar from grape marc after extraction), saving the consumption of this natural substrate. In addition, energy can be recovered from both hydrochar by combustion and from process water through anaerobic digestion to produce biogas. Hydrochars show good properties as solid fuel similar to lignite, with an energy content of around 27 MJ kg-1 (+30 % compared to the feedstock). The anaerobic digestion of the process water allowed obtaining up to 137 mL of biomethane per gram of fed COD. Finally, while HTC process waters are suitable for biological treatment, attention must be paid to the presence of inhibiting compounds that induce acute toxic effects in aerobic conditions. The proposed approach is consistent with the principles of circular economy and could increase the overall sustainability and resilience of the agro-industrial sector.

From process effluents to intestinal health promotion: Developing biopolymer-whey liposomes loaded with gingerol to heal intestinal wounds and neutralize oxidative stress.

As a sustainable strategy to valorize the main effluent of the cheese industry and potent environmental pollutant, whey, several biopolymer-whey vesicles loaded with gingerol were tailored for counteracting intestinal oxidative stress and boosting wound healing. An eco-friendly method was used to combine whey with four different water-dispersible biopolymers (xanthan gum, tragacanth, Arabic gum and sodium alginate), phospholipid and a natural antioxidant (gingerol). The results of cryogenic transmission microscopy and dynamic light scattering indicated that the vesicles were mostly unilamellar and small in size (∼100 nm) with low polydispersity index, high negative zeta potential and ability to entrap a high amount of gingerol (up to 94%). The vesicles could maintain their structures in acidic and neutral media and Turbiscan® technology confirmed their stability during the storage. Vesicles prepared with whey and tragacanth exhibited the highest capability to protect intestinal cells from damages induced by hydrogen peroxide. When Arabic and tragacanth gums were added to the whey vesicles, the closure rate of the scratched area was fast and no trace of the wound was observed after 72 h of treatment. These promising findings could open a new horizon in the application of whey in nanomedicine for the treatment of intestinal damages.

Extraction of the antioxidant phytocomplex from wine-making by-products and sustainable loading in phospholipid vesicles specifically tailored for skin protection.

The present study is aimed at valorizing grape pomace, one of the most abundant winery-making by-products of the Mediterranean area, through the extraction of the main bioactive compounds from the skin of grape pomace and using them to manufacture innovative nanoformulations capable of both avoiding skin damages and promoting skincare. The phytochemicals were recovered through maceration in hydroethanolic solution. Catechin, quercetin, fisetin and gallic acid, which are known for their antioxidant power, were detected as the main compounds of the extract. Liposomes and phospholipid vesicles modified with glycerol or Montanov 82® or a combination of both, were used as carriers for the extract. The vesicles were small (~183 nm), slightly polydispersed (PI ≥ 0.28), and highly negatively charged (~-50 mV). The extract was loaded in high amounts in all vesicles (~100%) irrespective of their composition. The antioxidant activity of the extract, measured by using the DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, was 84 ± 1%, and slightly increased when loaded into the vesicles (~89%, P < 0.05). The grape pomace extract loaded vesicles were highly biocompatible and able to protect fibroblasts (3T3) from the oxidative stress induced by hydrogen peroxide.

Environmental life cycle assessment of polyhydroxyalkanoates production from cheese whey.

Cheese whey (CW) is the main by-product of the dairy industry and is often considered one of the main agro-industrial biowaste streams to handle, especially within the European Union, where the diary activities play an essential role in the agrarian economy. In the paper, Life Cycle Assessment (LCA) is used to analyse the feasibility of producing polyhydroxyalkanoates (PHA) as the main output of an innovative CW valorisation route which is benchmarked against a conventional anaerobic digestion (AD) process. To this aim, the LCA inventory data are derived from lab-scale PHA accumulation tests performed on real CW, while data from the literature of concern are used for modelling both the PHA extraction from the accumulating biomass and for the alternative CW valorisation through AD. The comparison shows that AD would have better environmental performances than the baseline PHA production scenario. For example, the climate change indicator values result 44.8 and -35.7 kg CO2 eq./t CW for the baseline PHA recovery and AD, respectively. LCA proved to be a useful tool to highlight the weak points of innovative processes and suggest proper improvements. Once improved and again analysed through the LCA, the PHA production process from CW shows that environmental performance comparable to AD may be achieved. With reference, again, to the climate change indicator the value can be reduced to -50.3 kg CO2 eq./t CW for the improved PHA production process.

The dairy biorefinery: Integrating treatment processes for cheese whey valorisation.

With an estimated worldwide production of 190 billion kg per year, and due to its high organic load, cheese whey represents a huge opportunity for bioenergy and biochemicals production. Several physical, chemical and biological processes have been proposed to valorise cheese whey by producing biofuels (methane, hydrogen, and ethanol), electric energy, and/or chemical commodities (carboxylic acids, proteins, and biopolymers). A biorefinery concept, in which several value-added products are obtained from cheese whey through a cascade of biotechnological processes, is an opportunity for increasing the product spectrum of dairy industries while allowing for sustainable management of the residual streams and reducing disposal costs for the final residues. This review critically analyses the different treatment options available for energy and materials recovery from cheese whey, their combinations and perspectives for implementation. Thus, instead of focusing on a specific valorisation platform, in the present review the most relevant aspects of each strategy are analysed to support the integration of different routes, in order to identify the most appropriate treatment train.

Organic waste biorefineries: Looking towards implementation.

The concept of biorefinery expands the possibilities to extract value from organic matter in form of either bespoke crops or organic waste. The viability of biorefinery schemes depends on the recovery of higher-value chemicals with potential for a wide distribution and an untapped marketability. The feasibility of biorefining organic waste is enhanced by the fact that the biorefinery will typically receive a waste management fee for accepting organic waste. The development and implementation of waste biorefinery concepts can open up a wide array of possibilities to shift waste management towards higher sustainability. However, barriers encompassing environmental, technical, economic, logistic, social and legislative aspects need to be overcome. For instance, waste biorefineries are likely to be complex systems due to the variability, heterogeneity and low purity of waste materials as opposed to dedicated biomasses. This article discusses the drivers that can make the biorefinery concept applicable to waste management and the possibilities for its development to full scale. Technological, strategic and market constraints affect the successful implementations of these systems. Fluctuations in waste characteristics, the level of contamination in the organic waste fraction, the proximity of the organic waste resource, the markets for the biorefinery products, the potential for integration with other industrial processes and disposal of final residues are all critical aspects requiring detailed analysis. Furthermore, interventions from policy makers are necessary to foster sustainable bio-based solutions for waste management.

Propionate Production by Bioelectrochemically-Assisted Lactate Fermentation and Simultaneous CO2 Recycling.

Production of volatile fatty acids (VFAs), fundamental building blocks for the chemical industry, depends on fossil fuels but organic waste is an emerging alternative substrate. Lactate produced from sugar-containing waste streams can be further processed to VFAs. In this study, electrofermentation (EF) in a two-chamber cell is proposed to enhance propionate production via lactate fermentation. At an initial pH of 5, an applied potential of -1 V vs. Ag/AgCl favored propionate production over butyrate from 20 mM lactate (with respect to non-electrochemical control incubations), due to the pH buffering effect of the cathode electrode, with production rates up to 5.9 mM d-1 (0.44 g L-1 d-1). Microbial community analysis confirmed the enrichment of propionate-producing microorganisms, such as Tyzzerella sp. and Propionibacterium sp. Organisms commonly found in microbial electrosynthesis reactors, such as Desulfovibrio sp. and Acetobacterium sp., were also abundant at the cathode, indicating their involvement in recycling CO2 produced by lactate fermentation into acetate, as confirmed by stoichiometric calculations. Propionate was the main product of lactate fermentation at substrate concentrations up to 150 mM, with a highest production rate of 12.9 mM d-1 (0.96 g L-1 d-1) and a yield of 0.48 mol mol-1 lactate consumed. Furthermore, as high as 81% of the lactate consumed (in terms of carbon) was recovered as soluble product, highlighting the potential for EF application with high-carbon waste streams, such as cheese whey or other food wastes. In summary, EF can be applied to control lactate fermentation toward propionate production and to recycle the resulting CO2 into acetate, increasing the VFA yield and avoiding carbon emissions and addition of chemicals for pH control.

A parametric response surface study of fermentative hydrogen production from cheese whey.

Batch factorial experiments were performed on cheese whey+wastewater sludge mixtures to evaluate the influence of pH and the inoculum-to-substrate ratio (ISR) on fermentative H2 production and build a related predictive model. ISR and pH affected H2 potential and rate, and the fermentation pathways. The specific H2 yield varied from 61 (ISR=0, pH=7.0) to 371L H2/kg TOCwhey (ISR=1.44gVS/g TOC, pH=5.5). The process duration range was 5.3 (ISR=1.44gVS/g TOC, pH=7.5) - 183h (ISR=0, pH=5.5). The metabolic products included mainly acetate and butyrate followed by ethanol, while propionate was only observed once H2 production had significantly decreased. The multiple metabolic products suggested that the process was governed by several fermentation pathways, presumably overlapping and mutually competing, reducing the conversion yield into H2 compared to that expected with clostridial fermentation.

Biomass ash reutilisation as an additive in the composting process of organic fraction of municipal solid waste.

In this work the effects of selected types of biomass ash on the composting process and final product quality were studied by conducting a 96-day long experiment where the source separated organic fraction of municipal waste, mixed with wood prunings that served as bulking agent, was added with 0%, 2%, 4% and 8% wt/wt of biomass ash. The evolution over time of the main process parameters was observed, and the final composts were characterised. On the basis of the results, both the composting process and the quality of the final product were improved by ash addition. Enhanced volatile solids reduction and biological stability (up to 32% and 52%, respectively, as compared to the unamended product) were attained when ash was added, since ash favored the aerobic degradation by acting asa physical conditioner. In the final products, higher humification of organic matter (expressed in terms of the humification index, that was 2.25 times higher in the most-enriched compost than in the unamended one) and total Ca, K, Mg and P content were observed when ash was used. The latter aspect may influence the composts marketability positively, particularly with regards to potassium and phosphorus. The heavy metals content, that is regarded as the main environmental disadvantage when using ash asa composting additive, did not negatively affect the final composts quality. However, some other controversial effects of ash, related to the moisture and temperature values attained during the process, pH (8.8-9.2 as compared to 8.2 of the unamended compost) and electrical conductivity levels (up to 53% higher as compared to the unamended compost) in the final composts, were also observed.

Energy recovery from one- and two-stage anaerobic digestion of food waste.

One- and two-stage anaerobic digestion of food waste aimed at recovering methane (CH4) and hydrogen and methane (H2+CH4), respectively, were compared in order to assess the potential benefits from the two-stage process in terms of overall energy recovery. Results suggest that a two-stage process where the first reactor is properly operated in order to achieve a significant net hydrogen production, may display a 20% comparatively higher energy recovery yield as a result, mainly, of enhanced methane production as well as of the associated hydrogen production. The highest methane production of the two-stage process was due to improved hydrolysis and fermentation of food waste, with increased amounts of volatile fatty acids being readily available to methanogenesis.

Enhanced electrokinetic treatment of marine sediments contaminated by heavy metals and PAHs.

Dredged sediments contaminated by heavy metals and PAHs were subjected to both unenhanced and enhanced electrokinetic remediation under different operating conditions, obtained by varying the applied voltage and the type of conditioning agent used at the electrode compartments in individual experiments. While metals were not appreciably mobilized as a result of the unenhanced process, metal removal was found to be significantly improved when both the anodic and cathodic reservoirs were conditioned with the chelating agent EDTA, with removal yields ranging from 28% to 84% depending on the contaminant concerned. As for the effect on organic contaminants, under the conditions tested the electrokinetic treatment displayed a poor removal capacity towards PAHs, even when a surfactant (Tween 80) was used to promote contaminant mobilization, indicating the need for further investigation on this issue. Further research on organics removal from this type of materials through electrokinetic remediation is thus required. Furthermore, a number of technical and environmental issues will also require a careful evaluation with a view to full-scale implementation of electrokinetic sediment remediation. These include controlling side effects during the treatment (such as anodic precipitation, oxidation of the conditioning agent, and evolution of toxic gases), as well as evaluating the potential ecotoxicological effects of the chemical agents used.

A direct comparison amongst different technologies (aerobic granular sludge, SBR and MBR) for the treatment of wastewater contaminated by 4-chlorophenol.

Environmental concern on chlorinated phenols is rising due to their extreme toxicity even at low concentrations and their persistency in water and soils. Since the high amount of published data often lacks in terms of uniformity, direct comparisons amongst different treatment technologies are very difficult, or even impossible. In this study, granular sludge developed in an acetate-fed Granular sludge Sequencing Batch Reactor (GSBR) was used for the aerobic degradation of low chlorinated 4-chlorophenol (4CP), with readily biodegradable sodium acetate (NaAc) as growth substrate. A conventional Sequencing Batch Reactor (SBR) and a Membrane BioReactor (MBR) were operated in parallel under the same 4CP influent concentrations and/or 4CP volumetric organic loading rates as the GSBR, in order to carry out a direct comparison in terms of 4CP removal efficiencies and specific removal rates, effluent quality, waste sludge production, system simplicity, land area requirement, start-up times, NaAc dosage as growth substrate and maximum applied 4CP volumetric organic loading rate. A decision matrix was built to define the best technology to suit different scenarios: the GSBR was proved to be the most suitable technology when system simplicity, low land area requirement and short start-up times were considered as critical parameters for decision making.

Enhanced electrokinetic treatment of different marine sediments contaminated by heavy metals.

In the present work, the application of an assisted electrokinetic process for the removal of heavy metals from real contaminated sediments was investigated. The process made use of both chemical and physical methods, including addition of chelating and acid agents, as well as application of a hydraulic gradient. Lab-scale electrokinetic runs were applied on two different dredged sediments varying the applied voltage gradient and the treatment duration. The use of EDTA significantly improved the overall performance of the electrokinetic treatment for sediment V (more than 60% mobilized for each metal), while only aggressive acid conditioning with nitric acid was able to remove significant amounts of heavy metals (up to 40.5% for Pb) from sediment S due to the strong buffering capacity of this material. This clearly assesses that the specific characteristics of the materials under concern and the reactions occurring at the electrodes must be carefully evaluated when applying an electroremediation process.

Chromate adsorption in a transformed red mud permeable reactive barrier using electrokinesis.

Electrokinetic removal of chromium from artificially contaminated clayey soil in a bench-scale cell was enhanced by a reactive barrier (RB) of transformed Red Mud (TRM, BAUXSOL) fitted near the anode. When using 0.75 wt% of TRM in a soil spiked up to a Cr (VI) concentration of 1000 mg/kg dry weight, about 54 wt% of total Cr was removed after 6 days, as compared to only 36 wt% from the control cell without TRM RB. Increasing the duration of the treatment up to 12 days and using 1.5 wt% of TRM resulted in the increase of the above mentioned removal efficiencies up to 93 wt% and 57 wt%, respectively. The reduction of Cr (VI) into less mobile Cr (III) was limited by the TRM RB; in fact, lower reduction percentages for the tests with TRM RB as compared to the tests without were observed (respectively, 42% versus 72% for 6 days test, and 60% versus 93% for 12 days test). The obtained results show that Cr (VI) removal efficiency is proportional to the duration of the treatment and enhanced by the development of favorable pH conditions caused by the activity of the anodic TRM RB. It is suggested that electrokinesis may increase the efficiency of metal-oxyanion adsorption on TRM, as well as that the use of a TRM RB may improve the efficiency of contaminated soil remediation by means of electrokinesis.

Use of stone wool by-products in the construction of sanitary landfills and tailing dams.

The delay of the introduction of a used item or residual material in the "waste circuit" is a key factor of an effective and environmentally sound waste management policy. This principle has been fully adopted by the European legislation and consequently, in most of the member countries. In the same time, re-use of low cost materials or, even better, by-products in environment protection works (sanitary landfills, mineral processing residues dams, etc.) could make easier the effective implementation of an environmentally sound waste management policy, especially in developing countries. However, the assessment of the recovery options has to be performed on the basis of proper technical specifications concerning the kind of reuse proposed and of an accurate investigation on the technical and environmental properties of the residue. The present article reports the results of a research programme aiming at evaluating the feasibility of use of stone wool by-products, usually directly disposed in landfills, as construction materials for sanitary landfills and tailing dams.

Selection of plants for zinc and lead phytoremediation.

This article describes a laboratory scale experiment of phytoremediation that has the aim to identify a vegetable species that is able to survive to the high metal concentration of soil taken from Montevecchio, Sardinia, and verify how metal content in soil can influence phytoextraction performances. The experiments allowed finding a plant (Festuca arundinacea) that is able to tolerate Montevecchio soil and to accumulate high quantities of Zinc in the shoots and to evaluate Helichrysum italicum and Mirabilis jalapa performances in zinc and lead phytoextraction.