[The impact of gen polymorphism of subunits of receptors of thrombocytes GP IIb/IIIa on variation of indicators of thrombocyte hemostasis in obstetrics practice].

The article presents the results of study of the impact of gen polymorphism of subunits of receptors of thrombocytes GP Ilb/IIIa on variation of quantitative, functional and morphometric parameters of thrombocytes in 408 puerperae. The heterozygous variant of mutation was established in women with physiologically progress of pregnancy and delivery and in women with clinical manifestations of thrombophilia. The homozygous variant was established only in women with thrombophilia. The relationship is established between the presence of hetero- and homozygous mutations of gen of subunits of receptors of thrombocytes GP IIb/IIIa and inclination to hypercoagulation. This relationship manifests itself in increasing of degree of thrombocytes aggregation with all inductors and as well as in increasing of functional activity and appearance of young active cells, according morphometric analysis data. The application of computer morphometry of thrombocytes in the study made it possible to ascertain the activization of thrombocyte component of hemostasis in healthy women and availability of heterozygous mutation of gen of subunits of receptors of thrombocytes GP IIb/IIIa (PlA1/PlA2).

[Oil-oxidizing potential of associative rhizobacteria of the genus Azospirillum].

The oil-oxidizing potential of associative rhizobacteria of the genus Azospirillum was studied under laboratory conditions. After screening, A. brasilense strain SR80 was chosen for further investigation. The strain was capable of degrading 56.5% of crude oil (added in a concentration of 1%) over 14 days in a medium containing malate as an additional source of carbon and energy. Studies of associative properties showed that the strain had positive chemotaxis to wheat root exudates, colonized wheat roots, and produced indolyl-3-acetic acid. The synthesis of indolyl-3-acetic acid was not inhibited by oil. Under hydroponic conditions, crude oil stimulated growth of A. brasilense SR80, which promoted development of the wheat root system in the presence of oil and enhanced the level of oil degradation by the plant-microbial association.

[Efficiency of alfalfa and reed in the phytoremediation of hydrocarbon polluted soil]. / Ispol'zovanie liutserny i trostnika dlia fitoremidiatsii zagriaznennogo uglevodorodami grunta.

The efficacy of plants as means of decontaminating hydrocarbon-polluted soil has been studied. Ditch reed (Phragmites australis) and alfalfa (Medicago sativa) markedly intensified processes of pollutant destruction, the effect being particularly pronounced in the case of polycyclic aromatic hydrocarbons (PAHs). Comparative analysis of microflora in soils (including those devoid of plants and rhizosphere) demonstrated that, in addition to preventing the pollutant-induced decrease in the amount of heterotrophic microorganisms, the plants stimulated their development, significantly increasing the population of destructors. Effects of plants on major physiological groups of soil microorganisms under conditions of pollution were ambiguous. The rhizosphere consortium of alfalfa was less susceptible to effects of pollutants than that of reed.

[Degradation of mineral oil by Acinetobacter calcoaceticus strain]. / Degradatsiia mineral'nogo masla shtammom Acinetobacter calcoaceticus.

The Acinetobacter calcoaceticus strain TM-31 has been isolated from a microbial assemblage of a pilot plant purifying waste water polluted with mineral oil. This strain is capable of efficient degradation of components of mineral oil (alkanes, isoalkanes, and alkyl residues of the naphthene and arene fraction. The strain bears stably inherited plasmids of sizes 120, 9, and 8 kb, which can be transferred into plasmid-free cells of the parental strain and into bacteria of the genus Pseudomonas and ensure the degradation of hexadecane and mineral oil.

[Strain of Pseudomonas aeruginosa--producer of bioPAV]. / Shtamm Pseudomonas aeruginosa--produtsent bioPAV.

A Pseudomonas aeruginosa strain producing an extracellular surfactant (biosurfactant) was isolated. The growth of this strain, referred to as 50.3, on a mineral glycerol-containing medium produces an emulsifying activity (60%) and decreases the surface tension of the culture liquid by a factor of 2.8 (to 25 mN/m). The optimum conditions for its growth and production of biosurfactants: intense aeration, pH 7.0-8.0, and the presence of Mg2+. The optimum biosurfactant properties were achieved when glucose was used as the only source of carbon and energy and NH4Cl was used as a source of nitrogen. The biosurfactant was isolated from the culture liquid by extraction and precipitation.

[Respiratory activity of bacteria Acinetobacter calcoaceticus TM-31 during assimilation of alkane hydrocarbons]. / Respiratornaia aktivnost' bakterii Acinetobacter calcoaceticus TM-31 pri assimiliatsii alkanovykh uglevodorodov.

The respiratory activity of Acinetobacter calcoaceticus TM-31 with resect to alkane hydrocarbons was studied. The dynamics of oxygen consumption by the cells while assimilating n-hexadecane was assayed by a modified technique using an oxygen electrode. The dependence of cell respiratory activity on the amount of n-hexadecane within the concentration range of 0.03-0.66% was determined. It was demonstrated that the cells also displayed respiratory activity towards other medium-chain n-alkanes: hexane, octane, decane, tridecane, and heptadecane. Thus, we demonstrated the possibility of determining alkanes by measuring the respiratory activities of microorganisms.