ABSTRACT
AIM: Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact 30. MATERIALS AND METHODS: Species identification of 18,400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bacteriologic analyzer VITEK2 Compact30 (BioMerieuX, France) and MALDI-TOF-MS analysis method on AutofleXIII (Bruker Daltonics, Germany) mass-spectrometer. RESULTS: Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), ß-hemolytiC staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%). CONCLUSION: Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed; cost and authenticity of identification of a wide spectrum of microorganisms.
Subject(s)
Academic Medical Centers/methods , Bacteria/classification , Bacterial Typing Techniques/instrumentation , Fungi/classification , Mycological Typing Techniques/instrumentation , RNA, Ribosomal/genetics , Bacteria/genetics , Bacteria/isolation & purification , Female , Fungi/genetics , Fungi/isolation & purification , Gynecology , Humans , Infant, Newborn , Obstetrics , Perinatology , Pregnancy , RNA, Ribosomal/isolation & purification , Sequence Analysis, RNA , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Vagina/microbiologyABSTRACT
OBJECTIVE: Estimation of microbiocenosis of lower and upper genital tract in different morphological forms of chronic endometritis and endometrial polyps. MATERIAL AND METHODS: Histological examination of endometrial aspirates and microview of the lower and upper genital tract in 164 women of reproductive age with different character of menstrual and reproductive history. RESULTS: The risk of endometrial colonization in, disturbance microecology of the vagina is 3.5 times higher than that, in patients with normosenosis (p<0.01, OP=3.5 [95%. CI 1.63-8.11]). CONCLUSION: The microbiological diagnosis can be considered as a component of comprehensive diagnostics necessary to choose the appropriate management of patients with CE and PE.
