[The study of quality characteristics of the hydrogel ointments and films based on copolymers divinyl esters of diethylene glycol].

The possibility of using a hydrogel based on divinyl ether co- and terpolymer of diethylene glycol as the backbone polymer for incorporating water-soluble medicinal substances was examined. The character of the influence of emulsifiers, plasticizers, high-boiling liquids and bioactive substances is defined within the changes of physical-chemical properties of obtained hydrogels. The obtained polyelectrolyte hydrogels by their homogeneity, dehydration and rheological characteristics may be of concern in function of matrices to create external prolonged-action dosage forms.

[Oxidative modification of serum proteins in rats exposed to nonsymmetric dimethylhydrazine poisoning].

Studies of serum proteins modifications both spontaneous and catalyzed by metals in rats under single exposure to nonsymmetric dimethylhydrazine revealed reliable, significant increase in oxidative destruction of proteins. That proves deep peroxidative syndrome in the experimental animals.

[Effects of asymmetric dimethyl hydrazine on physical and chemical properties of blood plasma if experimental animals].

Asymmetric dimethyl hydrazine (ADH), a highly toxic propellant component, is known to cause metabolic disturbances in humans and laboratory animals. Focus of this investigation was placed on crystal-forming properties of blood serum, and types of plasma protein destructions in ADH-intoxicated rats. Object of investigation was blood plasma collected from rats exposed to 100 mg/kg of per oral ADH total (5 mg/kg per a day, 5 days a wk, 4 wks.). The crystal-forming ability of plasma was assessed with the teziographic technique. Severity of proteins destruction was determined by the content of carbonyl products of oxidative protein modification in plasma. Chronic exposure to low ADH doses caused oxidative destruction of proteins in blood plasma and, consequently, significant degradation of the plasma ability to form crystals reflected in an altered zonal phase structure and phase bursting on teziograms. Symmetry gave way to pathological structures, e.g. petals and loops. Amorphous areas grew in size, bursting lines thinned out. Concretions became less numerous. These data suggest a close relationship between the impairments in physical and chemical properties of blood plasma of experimental animals by highly toxic asymmetric dimethyl hydrazine.

[Oxidative metabolism and crystal-forming properties of blood of the experimental animals subjected to nonsymmetrical dimethylhydrazine].

We have studied parameters of oxidative metabolism and crystal-forming properties of the rat blood under conditions of intoxication by nonsymmetrical dimethylhydrazine (NDMH). We have found the increase of secondary and end-products of lipid peroxidation in blood erythrocytes 7 days after NDMH injection at a dose of 5 mg/kg. Administration of a higher dose of NDMH (70 mg/kg) results in essential accumulation of initial, secondary and end-products of lipid peroxidation in rat erythrocytes, accumulation of products of protein oxidative modification in blood plasma. Studies of plasma crystal-forming properties have shown, that the increase of a dose of the injected toxicant causes the loss of ash value and symmetry in arrangement of basic elements of facies (concretions, separateness, cracks), changes of their shape or their full disappearance. There are abnormal fields (individual or plural tongue structures, "wrinkles", sheet structures, garrot-like structures etc.). Thus the bright picture of the plasma structure-forming properties is noted already at low NDMH doses. The results obtained suggest close interrelation between the impairments of physical and chemical properties of blood plasma in experimental animals under canditions of toxic action of NDMH.

[The influence of cytaphate on the status of oxidative metabolism in rats with diabetic nephropathy].

The antioxidant effect of cytaphate (0,0-dimethyl-N-cytisinyl phosphate) has been studied in outbred white rats with alloxan-induced insulin-dependent diabetes mellitus. The content of malonic dialdehyde, diene conjugates, ketodienes, middle-weight molecules, primary and secondary products of lipid peroxidation (LPO), and Schiff bases and the activity of catalase, glutathione peroxidase, and adenosine deaminase in erytrocytes have been evaluated. The state of oxidative protein modification, the content of malonic dialdehyde and diene conjugates, and the superoxide-anion production in the blood plasma have been determined. In the blood of animals with model diabetes, the LPO and oxidative protein modification processes were intensified and the level of superoxide anion production was increased. The administration of cytaphate led to a decrease of the LPO intensity and oxidative protein modification and to normalization of the level of superoxide anion production and the activity of enzymes involved in the antioxidant protection system.

[Effects of nutrition characteristics on oxidative metabolism in electric welders]. / Vliianie kharaktera pitaniia na okislitel'nyi metabolizm u élektrosvarshchikov.

Analysis of actual feeding and of oxidative metabolism in electric welders of enterprises was carried out. On the basis of obtained data the hypothesis concerning mechanism of oxidative metabolism violation developing in these conditions (oxidative stress) and its consequences for health is offered. The findings of investigation can be used of in development of products of a treatment-and-prophylactic feeding with antioxidative direction.

[Lipid peroxidation and antioxidant defense in renal affections of exotoxic etiology]. / Pereokisnoe okislenie lipidov i sistema antioksidatnoi zashchity pri porazheniiakh pochek ekzotoksicheskoi etiologii.

Exogenic poisoning is often complicated by toxic nephropathy pathogenesis of which remains obscure. In view of this, the authors have performed a clinical study of lipid peroxidation (LPO) and antioxidant defense (AOD) in acute exogenic poisoning with renal lesion. Acute poisoning results in LPO activation and inhibition of AOD enzymes activity. Emerging toxic nephropathy aggravates disorders in the system LPO-AOD actively participating in pathogenesis of this complication.

[Changes in the pulmonary surfactant system and induced chemiluminescence of pulmonary washings after fasting and the effect of alpha-tocopherol on them]. / Izmeneniia surfaktantnoi sistemy legkikh i indutsirovannoi khemiliuminestsentsii legochnykh smyvov posle golodaniia i vliianie na nikh alpha-tokoferola.

A complex of methods was used to study the pulmonary surfactant system and the intensity of induced chemoluminescence of pulmonary washings in rats after 4-week fasting and the effect produced on them by alpha-tocopherol. During fasting changes occurred in the values of surface activity of pulmonary washings, extracts, stability coefficient, and intensity of induced chemoluminescence; this was evidence of exhaustion of the intracellular surfactant reserve and intensification of lipid peroxidation. Administration of alpha-tocopherol during fasting reduced the disturbances in the pulmonary surfactant system of the animals and the degree of increased lipid peroxidation activity which indicates that the antioxidant protects the unsaturated fatty acids from oxidation.