Pyramiding of insecticidal compounds for control of the cowpea bruchid (Callosobruchus maculatus F.).

The cowpea bruchid (Callosobruchus maculatus F.) (Chrysomelidae: Bruchini) is a major pest of stored cowpea grain. With limited available technologies for controlling the bruchid, transgenic cowpeas with bruchid resistance genes engineered into them could become the next management tools. An investigation was made of two different sets of potential transgenic insecticidal compounds using an artificial seed system: (i) CIP-PH-BT-J and recombinant egg white avidin, and (ii) avidin and wheat alpha-amylase inhibitor. CIP-PH-BT-J (0.1%; 1000 mg kg(-1)) and recombinant egg white avidin (0.006%; 60 mg kg(-1)) incorporated separately into artificial seeds caused 98.2 and 99% larval mortality rates respectively. Combining CIP-PH-BT-J and avidin in the same artificial seed provided additional mortality compared with each factor incorporated singly; no insects survived in seeds with the combined toxins. Similarly, when avidin and wheat alpha-amylase inhibitor (alphaAI) (1%; 10 g kg(-1)) were incorporated separately into artificial seeds, this caused 99.8 and 98% mortality respectively. However, in combination, avidin and alphaAI did not increase mortality, but they did cause a significant increase in developmental time of the cowpea bruchids. These results emphasize that the joint action of potential insecticidal compounds cannot be predicted from results obtained separately for each compound, and they suggest potential transgenes for further consideration.

Comparison of chemical characteristics of three soybean cysteine proteinase inhibitors.

Three recombinant soybean cysteine proteinase inhibitors (rSCPIs) L1, R1, and N2 were chemically characterized. These inhibitors have the potential to inhibit the growth and development of three major agricultural crop pests known to utilize cysteine proteinases (CPs) for protein digestion: Western corn rootworm, Colorado potato beetle, and cowpea weevil. Characterization data obtained show differences between the inhibitors and will be needed to consider the use of rSCPIs to create insect resistance in plants.