Subject(s)
Dermatitis/epidemiology , Leukemia, Lymphocytic, Chronic, B-Cell/pathology , Skin Neoplasms/epidemiology , Skin/pathology , Aged , Aged, 80 and over , Comorbidity , Dermatitis/genetics , Dermatitis/pathology , Disease Progression , Female , Genes, Immunoglobulin Heavy Chain/genetics , Humans , Leukemia, Lymphocytic, Chronic, B-Cell/epidemiology , Leukemia, Lymphocytic, Chronic, B-Cell/genetics , Male , Middle Aged , Mutation , Neoplasm Staging , Risk Factors , Skin Neoplasms/genetics , Skin Neoplasms/pathology , Tumor Suppressor Protein p53/geneticsSubject(s)
Burkitt Lymphoma/genetics , DNA Methylation , Herpesvirus 4, Human , High-Throughput Nucleotide Sequencing , Burkitt Lymphoma/virology , Cell Line, Tumor , Exome , Gene Expression Regulation, Neoplastic , Herpesvirus 4, Human/genetics , High-Throughput Nucleotide Sequencing/methods , Humans , Karyotyping , Polymorphism, Single NucleotideABSTRACT
The recently discovered MLT/MALT1 gene is fused with the API2 gene in the t(11;18)(q21;q21), which characterizes about one-third of MALT lymphomas. In order to screen for variant translocations and amplifications of MLT/MALT1, we have developed a novel, undirected two-color interphase fluorescence in situ hybridization (FISH) assay with two PAC clones flanking MLT/MALT1. This assay was applied to 108 marginal zone B-cell lymphomas (MZBCLs), including 72 extranodal MALT lymphomas, 17 nodal, and 19 splenic MZBCL. In 19 MALT lymphomas (26%), but in none of the nodal or splenic MZBCL, separated hybridization signals of the MLT/MALT1 flanking probes, were found. Further FISH analyses showed that 12 of these 19 cases displayed the classical t(11;18) and the remaining seven cases revealed the novel t(14;18)(q32;q21), involving the MLT/MALT1 and IGH genes. The frequency at which these translocations occurred varied significantly with the primary location of disease. The t(11;18) was mainly detected in gastrointestinal MALT lymphomas, whereas the t(14;18) occurred in MALT lymphomas of the parotid gland and the conjunctiva. Amplification of MLT/MALT1 was not observed in any of the lymphomas analyzed. We conclude that the translocations t(11;18)(q21;q21) and t(14;18)(q21;q32) represent the main structural aberrations involving MLT/MALT1 in MALT lymphomas, whereas true amplifications of MLT/MALT1 occur rarely in MZBCL.
