The effects of seasonality, management, infrastructure, and automation on the milking efficiency of herringbone and rotary milking parlors in Ireland.

The objective of this study was to document the milking efficiency of a sample of Irish dairy farms and to understand the effects of (1) seasonality, (2) management practices, (3) parlor infrastructure, and (4) parlor automations on milking efficiency metrics. A novel methodology based on empirical data from video cameras, infrastructure surveys, and milk yield data allowed for the accurate computation of milking efficiency metrics and quantification of the effects of seasonality, number of operators, and parlor automations on milking efficiency across 2 parlor types. The data for this study were collected over 2 periods: period 1 (July 28, 2020, to October 23, 2020, peak-late production) and period 2 (April 12, 2021, to May 19, 2021, early-peak production) from a sample of 16 herringbone and 10 rotary commercial Irish dairy farms. Milking efficiency was evaluated on each farm using 3 key performance indicators: (1) cows milked per hour (cows/h), (2) cows milked per operator per hour (cows/h per operator), and (3) liters of milk harvested per hour (L/h). Milking efficiency key performance indicators were calculated using "total process time," defined as the time between the first cow entering the holding yard and the end of the cleaning process. Average herd sizes for herringbone and rotary farms were 180 and 425 cows, respectively. Average system sizes for herringbone and rotary farms were 20 and 50 clusters, respectively. For herringbone farms, the average milking efficiency was 94 cows/h, 73 cows/h per operator, and 1,012 L/h, whereas rotary farms achieved an average milking efficiency of 170 cows/h, 132 cows/h per operator, and 1,534 L/h. Parlor size was strongly correlated with milking efficiency (cows/h) for herringbone parlors (0.91) but was only moderately correlated for rotary parlors (0.50). Hence, we documented the effect of parlor size on milking efficiency is relative to parlor type. Cluster utilization values on herringbone farms were 5 cows/cluster per h, 4 cows/cluster per operator per h, and 51 L/cluster per h, which were 67%, 33%, and 65% greater than rotary farms, respectively. We found for both herringbone and rotary farms hourly cow throughput (cows/h, cows/h per operator) were greatest during period 1 and that the volume of milk harvested per hour (L/h) was greatest for period 2. Thus, we documented an inverse seasonal relationship between hourly rates of cows milked and milk harvested. We observed that for herringbone farms, milking efficiency (cows/h, L/h) had a strong positive correlation (0.75, 0.74) with the levels of automation use. However, the minimal variation in automations used among rotary farms made it difficult to evaluate their effect on milking efficiency. Similarly, we found that the effect of automations on milking efficiency was dependent on parlor type. On average, a second operator at milking for both herringbone (H) and rotary (R) farms increased values for cows/h (+19%, H; +34%, R) and L/h (+21%, H; +12%, R) but lowered values for cows/h per operator (-35%, H; -12%, R). The holistic methodology applied in this study allowed us to add novel data to the literature by quantifying the effects of seasonality, the number of operators present at milking, and parlor automation use on milking efficiency across 2 parlor types.

Convergent actions of stress and stimulants via epigenetic regulation of neural circuitry.

The dorsal striatum integrates prior and current information to guide appropriate decision-making. Chronic stress and stimulant exposure interferes with decision-making, and can confer similar cognitive and behavioral inflexibilities. This review examines the literature on acute and chronic regulation of the epigenome by stress and stimulants. Recent evidence suggests that exposures to stress and stimulants share similarities in the manners in which they regulate the dorsal striatum epigenome through DNA methylation, transposable element activity, and histone post-translational modifications. These findings suggest that chronic stress and stimulant exposure leads to the accumulation of epigenetic modifications that impair immediate and future neuron function and activity. Such epigenetic mechanisms represent potential therapeutic targets for ameliorating convergent symptoms of stress and addiction.

A scenario for writing creative scenarios.

This paper examines the elements of a pathway to writing the bold, innovative scenarios necessary to promote sustainable socio-ecological integration. Innovative scenarios incorporate three virtues essential to making knowledge systemically useful: creativity, collaboration, and communication. The main features of such a scenario-writing process include: (1) the effective integration of participants from a diverse array of disciplinary perspectives; (2) an integrated approach to defining problems as a system of interrelated structures, functions, and processes; (3) the collaborative learning required to synthesize a comprehensive understanding of the interrelated components and processes of the system containing the problem(s); (4) a process of co-writing that guides the alteration of socio-ecological systems to eliminate the problem(s); and (5) communication that clearly and persuasively conveys the message required to actualize resolution.

Over 20 Years of Machine Learning Applications on Dairy Farms: A Comprehensive Mapping Study.

Machine learning applications are becoming more ubiquitous in dairy farming decision support applications in areas such as feeding, animal husbandry, healthcare, animal behavior, milking and resource management. Thus, the objective of this mapping study was to collate and assess studies published in journals and conference proceedings between 1999 and 2021, which applied machine learning algorithms to dairy farming-related problems to identify trends in the geographical origins of data, as well as the algorithms, features and evaluation metrics and methods used. This mapping study was carried out in line with PRISMA guidelines, with six pre-defined research questions (RQ) and a broad and unbiased search strategy that explored five databases. In total, 129 publications passed the pre-defined selection criteria, from which relevant data required to answer each RQ were extracted and analyzed. This study found that Europe (43% of studies) produced the largest number of publications (RQ1), while the largest number of articles were published in the Computers and Electronics in Agriculture journal (21%) (RQ2). The largest number of studies addressed problems related to the physiology and health of dairy cows (32%) (RQ3), while the most frequently employed feature data were derived from sensors (48%) (RQ4). The largest number of studies employed tree-based algorithms (54%) (RQ5), while RMSE (56%) (regression) and accuracy (77%) (classification) were the most frequently employed metrics used, and hold-out cross-validation (39%) was the most frequently employed evaluation method (RQ6). Since 2018, there has been more than a sevenfold increase in the number of studies that focused on the physiology and health of dairy cows, compared to almost a threefold increase in the overall number of publications, suggesting an increased focus on this subdomain. In addition, a fivefold increase in the number of publications that employed neural network algorithms was identified since 2018, in comparison to a threefold increase in the use of both tree-based algorithms and statistical regression algorithms, suggesting an increasing utilization of neural network-based algorithms.

Distinct cortical-amygdala projections drive reward value encoding and retrieval.

The value of an anticipated rewarding event is a crucial component of the decision to engage in its pursuit. But little is known of the networks responsible for encoding and retrieving this value. By using biosensors and pharmacological manipulations, we found that basolateral amygdala (BLA) glutamatergic activity tracks and mediates encoding and retrieval of the state-dependent incentive value of a palatable food reward. Projection-specific, bidirectional chemogenetic and optogenetic manipulations revealed that the orbitofrontal cortex (OFC) supports the BLA in these processes. Critically, the function of ventrolateral and medial OFC→BLA projections is doubly dissociable. Whereas lateral OFC→BLA projections are necessary and sufficient for encoding of the positive value of a reward, medial OFC→BLA projections are necessary and sufficient for retrieving this value from memory. These data reveal a new circuit for adaptive reward valuation and pursuit and provide insight into the dysfunction in these processes that characterizes myriad psychiatric diseases.

Habits Are Negatively Regulated by Histone Deacetylase 3 in the Dorsal Striatum.

BACKGROUND: Optimal behavior and decision making result from a balance of control between two strategies, one cognitive/goal-directed and one habitual. These systems are known to rely on the anatomically distinct dorsomedial and dorsolateral striatum, respectively. However, the transcriptional regulatory mechanisms required to learn and transition between these strategies are unknown. Here we examined the role of one chromatin-based transcriptional regulator, histone modification via histone deacetylases (HDACs), in this process. METHODS: We combined procedures that diagnose behavioral strategy in rats with pharmacological and viral-mediated HDAC manipulations, chromatin immunoprecipitation, and messenger RNA quantification. RESULTS: The results indicate that dorsal striatal HDAC3 activity constrains habit formation. Systemic HDAC inhibition following instrumental (lever press → reward) conditioning increased histone acetylation throughout the dorsal striatum and accelerated habitual control of behavior. HDAC3 was removed from the promoters of key learning-related genes in the dorsal striatum as habits formed with overtraining and with posttraining HDAC inhibition. Decreasing HDAC3 function, either by selective pharmacological inhibition or by expression of dominant-negative mutated HDAC3, in either the dorsolateral striatum or the dorsomedial striatum accelerated habit formation, while HDAC3 overexpression in either region prevented habit. CONCLUSIONS: These results challenge the strict dissociation between dorsomedial striatum and dorsolateral striatum function in goal-directed versus habitual behavioral control and identify dorsostriatal HDAC3 as a critical molecular directive of the transition to habit. Because this transition is disrupted in many neurodegenerative and psychiatric diseases, these data suggest a potential molecular mechanism for the negative behavioral symptoms of these conditions and a target for therapeutic intervention.

First X-ray crystal structure and internal reference diffusion-ordered NMR spectroscopy study of the prototypical Posner reagent, MeCu(SPh)Li(THF)3.

Grow slow: The usual direct treatment of MeLi and CuSPh did not yield X-ray quality crystals of MeCu(SPh)Li. An indirect method starting from Me2CuLi⋅LiSPh and chalcone afforded the desired crystals by the slow reaction of the intermediate π-complex (see scheme). This strategy produced the first X-ray crystal structure of a Posner cuprate. A complementary NMR study showed that the contact ion pair was also the main species in solution.

π-Complexes from acyl cyanides and lithium dimethylcuprate(I).

Rapid injection of pyruvonitrile or benzoyl cyanide into solutions of Me2CuLi in THF-d8 at -100 °C gave complexes that were stable at this temperature. 1D NMR with multiply labelled substrates ((13)C/(15)N) and 2D NMR ((1)H/(13)C) identified them as the first cuprate-carbonyl π-complexes.

Rapid injection NMR reveals η3 'π-allyl' Cu(III) intermediates in addition reactions of organocuprate reagents.

By using rapid injection NMR, it has now been possible to prepare and characterize the η(3) 'π-allyl' copper(III) intermediate that has been proposed for addition reactions of organocopper(I) reagents and α,ß-unsaturated carbonyl compounds.

Minimization of organocuprate complexity through self-organization: remarkable orientation effect in mixed cuprate π complexes.

They "know" where to go: a powerful orientation effect has been observed in complexes of mixed organocuprates [R(T)R(NT)CuLi] and substrates with C-C, C-N, and C-S double bonds (see scheme; Th=thienyl). The preferred geometry of the intermediate complex sets up the facile addition of R(T) to the double bond, rather than addition of the "dummy ligand", R(NT) .

Complexes of Gilman reagents with C-S and C-N double bonds: sigma or pi bonding?

Upon rapid injection, a variety of thiocarbonyl compounds react with the Gilman reagent Me(2)CuLi at -100 degrees C inside the probe of an NMR spectrometer to give high yields of complexes. Typical examples of substrates include carbon disulfide, methyl dithioacetate, methyl dithiobenzoate, thiobenzophenone, ethylene trithiocarbonate, and phenyl isothiocyanate. Evidence suggesting the formal oxidation state of copper in these complexes to be Cu(III) is presented. The last example was particularly interesting, since it involved a transient intermediate that was identified as a complex with a C-N double bond. Methyl isothiocyanate gave a stable C-N double-bond complex.

Serendipity strikes again--efficient preparation of lithium tetramethylcuprate(III) via rapid injection NMR.

Lithium tetramethylcuprate(III), Me(4)CuLi, the Cu(III) analog of the Gilman reagent, has been prepared in high yield from halo-Gilman reagents Me(2)CuLi.LiX (X = Cl, Br, I) and 2,3-dichloropropene and found to have surprising thermal stability. The cyano-Gilman reagent (X = CN) follows a different pathway.

Organocuprate(III) chemistry: synthesis and reactivity of amido, cyano, phosphido and thiolato ate complexes of copper(III).

Lithium tetramethylcuprate(III) 1 reacts readily at -100 degrees C with appropriate sources of H(+) or X(+) (X = Br, I) to remove a methyl and in some cases incorporate the counterion (e.g., arylthio or cyano) to give stable complexes. These derivatives can in turn serve as starting materials for other Cu(III) complexes.

Opening the 'black box': oscillations in organocuprate conjugate addition reactions.

Under the conditions of a rapid injection experiment, the conjugate addition reactions of butyl Gilman reagents with 2-cyclohexenone undergo oscillations of a complex nature.

Rapid-injection NMR study of iodo- and cyano-Gilman reagents with 2-cyclohexenone: observation of pi-complexes and their rates of formation.

Rapid-injection is a very useful technique for the preparation of temperature-sensitive and air-sensitive compounds in the cold, nitrogen-filled probe of an NMR spectrometer. We have used this method to prepare solutions of pi-complexes from 2-cyclohexenone and prototypical cuprates Me2CuLi.LiI and Me2CuLi.LiCN, and we have assigned structures on the basis of 1H and 13C NMR. In each case two pi-complexes were observed, and in the former, their rates of formation were measured by rapid-injection 1H NMR and EXSY spectroscopy. These results provide insights into the normal and anomalous conjugate addition reactions of organocuprates.