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1.
Mar Pollut Bull ; 204: 116562, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38850757

ABSTRACT

The rise of plastics in the 20th century revolutionized modern life but inadvertently exacerbated the marine litter crisis. The proliferation of wastes such as single-use plastics has escalated pollution along the coastline, demanding coordinated, decisive, and unified action. While the absence of specific national marine litter policies is concerning, there is growing recognition of urgency to address this issue. A group of experts and stakeholders was involved through a multi-staged workshop to assess the generated information based on scientific evidence and formulate a framework for the National Marine Litter Policy (NMLP). This paper proposes policy options (4 targets & 20 strategies) to address marine litter pollution, especially plastics, and aims to elucidate the urgency and significance of implementing an NMLP as a comprehensive strategy to combat plastics pollution. Implementing dedicated policies and action plans tailored to the unique challenges faced by each country is a vital step towards sustainable oceans.


Subject(s)
Environmental Policy , Plastics , India , Environmental Monitoring , Water Pollution/prevention & control , Water Pollution/statistics & numerical data , Waste Products
2.
Mar Environ Res ; 199: 106595, 2024 Jul.
Article in English | MEDLINE | ID: mdl-38879903

ABSTRACT

A massive fish mortality of the major species, viz., Mugil cephalus, Chanos chanos, and Oreochromis mossambicus, occurred on November 27, 2017 in the Adyar estuary. This catastrophe followed a spell of heavy rainfall and flash floods. A detailed study of water quality parameters (pH, water temperature, salinity, total suspended matter, dissolved oxygen, biochemical oxygen demand, and nutrients) and heavy metals, i.e., chromium (Cr), copper (Cu), cadmium (Cd), nickel (Ni), lead (Pb), and zinc (Zn), in the sediment and fish tissues were analyzed. Pollution indices like the enrichment factor (EF), geo-accumulation index (Igeo), contamination factor (CF), pollution load index (PLI), potential ecological risk index (PERI), and biota sediment accumulation factor (BSAF) were used to measure heavy metals. The pollution indices revealed that metals were significantly enriched in the sediments. The Igeo indicates that there was moderate contamination by Cd (2.27-3.25), whereas CF shows high contamination by Cd (7.22-9.72) and moderate contamination by Pb (2.5-3.25). The PLI (1.04-1.13) suggests that heavy metal contamination of sediments has occurred. Results showed that high concentrations of the toxic metals Pb and Cd were found in the sediment as well as in fish tissues. Length and weight of fish are significantly correlated (r2 = 0.98, p < 0.05). The study revealed that the mass fish kill was due to impulsive changes in the water quality and heavy metal pollution from untreated urban sewage discharges in this region.


Subject(s)
Environmental Monitoring , Estuaries , Fishes , Metals, Heavy , Water Pollutants, Chemical , Animals , Metals, Heavy/analysis , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/toxicity , India , Geologic Sediments/chemistry , Bays
4.
Protist ; 175(2): 126019, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38309038

ABSTRACT

The present investigation focused on studying the phylogenetic position of the green Noctiluca endosymbiont, Pedinomonas noctilucae, collected from the Gulf of Mannar, India. In this study, we re-examined the evolutionary position of this endosymbiotic algae using rbcL sequences. The phylogenetic analysis revealed that P. noctilucae is distantly related to the Pedinomonas species, and formed a monophyletic clade with Marsupiomandaceae. Based on the phylogenetic association of endosymbiont with Maruspiomonadales it was concluded that the endosymbiont belongs to an independent genus within the family Marsupiomonadaceae. At the site of the bloom, Noctiluca scintillans was found to exhibit a dense monospecific proliferation, with an average cell density of 27.l88 × 103 cells L-1. The investigation revealed that the green Noctiluca during its senescent phase primarily relied on autotrophic nutrition, which was confirmed by the presence of a high number of trophonts, vegetatively reproducing cells (1.45 × 103 cells L-1) and the absence of food vacuoles.


Subject(s)
Chlorophyta , Dinoflagellida , Phytoplankton , Phylogeny , Biological Evolution
5.
Mar Environ Res ; 191: 106161, 2023 Oct.
Article in English | MEDLINE | ID: mdl-37704539

ABSTRACT

In India, intertidal seaweed resources are widely investigated and utilized for various applications, whereas reef-associated seaweed resources and their impacts on corals are lesser known. Thus, the present study investigated the diversity and impacts of macroalgae and cyanobacteria on coral reefs distributed in 21 islands under the Gulf of Mannar Marine Biosphere Reserve (GoMMBR), Tamil Nadu. About 140 macroalgal species representing 53 species of Chlorophyta, 32 species of Ochrophyta (Phaeophyta), and 55 species of Rhodophyta were recorded. Only three cyanobacterial species were documented during this study. All the documented species were categorized as edible, medicinal, smothering, bloom-forming, sediment trapping, and auxiliary. Diversity indices and multivariate analysis indicated latitudinal gradient distribution of macroalgae, where the maximum diversity was observed from the Mandapam group of Islands. The predominant genera observed in all the islands were Caulerpa, Halimeda, Turbinaria, and Sargassum. The updated checklist of seaweeds and cyanobacteria of India revealed 1118 and 258 species, correspondingly, on Indian coasts, including coral reef regions. The use of traditional morphology-based techniques in this study without molecular approaches to identify all of the specimens limits our investigation. Thus, molecular taxonomy is necessary to revalidate and confirm the actual genetic diversity existing in the Indian waters. Results of this study would benefit the scientific community and industries in various aspects, such as molecular taxonomy, biomass utilization, reef conservation, and industrial applications.


Subject(s)
Anthozoa , Cyanobacteria , Phaeophyceae , Seaweed , Animals , Coral Reefs , India , Cyanobacteria/genetics , Ecosystem
6.
Environ Monit Assess ; 195(10): 1203, 2023 Sep 13.
Article in English | MEDLINE | ID: mdl-37702824

ABSTRACT

The spatial distribution of total petroleum hydrocarbons (TPH) were analysed in the seawater and sediment samples collected from 27 locations along the Southeast coast of India. A first-time assessment was carried out on the distribution of TPH in both water and sediments for the entire coastline of Tamil Nadu. The concentration of TPH in seawater showed large spatial variation ranging from below detection level (BDL) to 47.5 µg/L and 0.01 to 53.12 µg/L in the surface and bottom waters, respectively. TPH levels exceeded the regulatory limits specified by FAO, China's Marine Monitoring Standards and the European Community in the seawater samples of Thoothukudi harbour (S2 station). The results showed that seawaters of southern stations were comparatively more polluted with TPH. TPH values in sediment were between 2.33 and 30.07 µg/g, and their levels remained below the Marine Sediment Quality Standard (500 µg/g). The spatial profile of TPH in sediments were contrasting to that observed for seawater. Higher TPH values were observed in sediments of the northern region than southern. TPH contents are strongly correlated with clay (R2 = 0.776; P < 0.001) and silt (R2 = 0.648; P < 0.001); conversely, there is a significant negative correlation between TPH and sand (R2 = 0.753; P < 0.001). ANOVA analysis demonstrated a significant difference (F = 11.75; p < 0.01) between the TPH concentrations of water and sediments. Non-metric multidimensional scaling (nMDS) was performed to determine the similarity among sampling stations that formed five crusted groups. Sediment along the southeast coast can be categorised as slightly polluted with respect to TPH as per the ATSDR standards.


Subject(s)
Environmental Monitoring , Petroleum , India , Seawater , Water , Hydrocarbons
7.
Mar Pollut Bull ; 195: 115523, 2023 Oct.
Article in English | MEDLINE | ID: mdl-37716131

ABSTRACT

Dense and green-coloured patches were encountered on the sea surface waters of the Tuticorin coast on 22nd October 2022. Microscopic investigation revealed that the discoloration is caused by plankton, green Noctiluca scintillans. In order to find out the causes that trigger the bloom of N. scintillans, plankton samples were collected for 5 days in fourteen days duration from 22nd October to 4th November. During the peak bloom period, the abundance and biovolume of N. scintillans reached 1.56 × 104 cells/L and 21.8 × 1010µm3/L, respectively. The highest concentration (73.65 mg/m3) of chlorophyll-a was recorded during blooming period that was caused by Gonyaulax polygramma and endosymbiont, Pedinomonas noctilucae in N. scintillans. Formation of G. polygramma bloom is being reported for the first time in Tuticorin, southeast coast of India, with a species abundance of 36.9 × 104 cells/L. Present study concluded that besides the optimum hydrological conditions and eutrophic nature of the system, abundant prey (G. polygramma) facilitated the N. scintillans bloom.

8.
Indian J Occup Environ Med ; 27(2): 143-147, 2023.
Article in English | MEDLINE | ID: mdl-37600643

ABSTRACT

Background: Avian diseases should be known to people who raise birds as pets or for commercial purposes. Exposure to high levels of airborne dust and endotoxins is known to cause allergies. This study, therefore, intends to assess avian allergy and its associated factors among poultry workers and bird fanciers in urban Mysuru. Methods: A cross-sectional study was done over three months among 60 poultry workers and 60 bird fanciers in a selected rural population of the Mysuru district. The participants were selected by simple random sampling method and their data were obtained by interview method using a pre-tested semi-structured questionnaire. Chi-square test/Fisher's exact test was used to check the associations between the demographic variables' avian allergy. Results: The survey revealed that 31.67% of poultry workers and 53.33% of bird fanciers had symptoms. The majority of the study participants were illiterate and were not using personal protective equipment (PPE) regularly. The results showed a significant association between education (P = 0.013) and the use of PPE (P = 0.020) with avian allergy. Observing the health hazards, 42.10% had respiratory problems, 42.10% had skin problems, and 26.31% had eye problems. Further, a significant association was also found between education and knowledge about diseases (P = 0.001). Conclusion: The avian allergy among the bird fanciers was higher when compared to poultry workers. It is necessary to organize routine counseling, awareness, and training programs about diseases and prevention strategies related to their work and living environments and keep them updated.

9.
Heliyon ; 9(4): e15416, 2023 Apr.
Article in English | MEDLINE | ID: mdl-37151638

ABSTRACT

Melanoma is an abnormal proliferation of skin cells that arises and develops in most of the cases on surface of skin that is exposed to copious amounts of sunlight. This common type of cancer may develop in areas of the skin that are not exposed to a much abundant sunlight. The research addresses the problem of Segmentation and Classification of Melanoma Skin Cancer. Melanoma is the fifth most common skin cancer lesion. Bio-medical Imaging and Analysis has become more promising, interesting, and beneficial in recent years to address the eventual problems of Melanoma Skin Cancerous Tissues that may develop on Skin Surfaces. The evolved research finds that Attributes Selected for Classification with Color Layout Filter model. The research has produced an optimal result in terms of certain performance metrics accuracy, precision, recall, PRC (what is PRC? Expansion is needed in Abstract), The proposed method has yielded 90.96% of accuracy and 91% percent of precise and 0.91 of recall out of 1.0, 0.95 of ROC AUC, 0.87 of Kappa Statistic, 0.91 of F-Measure. It has been noticed a lowest error with reference to proposed method on certain dataset. Finally, this research recommends that the Attribute Selected Classifier by implementing one of the image enhancement techniques like Color Layout Filter is showing an efficient outcome.

10.
Indian J Tuberc ; 70(2): 226-231, 2023 Apr.
Article in English | MEDLINE | ID: mdl-37100580

ABSTRACT

INTRODUCTION: In the context of changing over from an intermittent treatment regimen to a daily regimen, it becomes crucial to understand the impact of a daily regimen on the treatment process and outcome. It enables health professionals to strengthen strategies, to enhance the quality of treatment as well as the quality of life of TB patients. The perspective of each stakeholder involved in the process is important in assessing the impact of the daily regimen. OBJECTIVES: To understand patients' and providers' perspectives on the daily regimen of Tuberculosis treatment. METHODOLOGY: A qualitative study was conducted between March 2020 to June 2020, including in-depth interviews with TB patients on treatment and DOT providers, and Key Informant Interview(KII) with TB Health Visitors(TBHV) and family members of TB patients. A thematic-network analysis approach was utilized to get the results. RESULTS: Two sub-themes emerged: (i) Acceptance of the daily regimen of treatment; (ii) operational difficulties of the daily regimen. No injections in the regimen, fewer side effects of drugs as dose depends on weight band, family members can be treatment supporter, awareness about disease and treatment, the drugs are as same as private drugs available, adherence has improved, monthly DBT were found to some of the enablers in the study. The Barriers found in the study were traveling daily to get drugs, loss of daily wages, accompanying patients daily, tracing private patients, pyridoxine is not given free in this regimen, increased workload for treatment providers, etc. CONCLUSION: The study points out that acceptance of the patient to the daily regimen is better as they have lesser side effects. The operational difficulties in the implementation of the daily regimen can be addressed by providing family members as treatment supporters.


Subject(s)
Drug-Related Side Effects and Adverse Reactions , Tuberculosis , Humans , Tuberculosis/drug therapy , Antitubercular Agents , Quality of Life , Qualitative Research , Medication Adherence , Drug-Related Side Effects and Adverse Reactions/drug therapy
11.
Mar Pollut Bull ; 190: 114838, 2023 May.
Article in English | MEDLINE | ID: mdl-37002963

ABSTRACT

Seven expeditions were carried out during pre-monsoon, monsoon and post monsoon in 2018-2019 for marine plastic collection in surface waters of Northern Indian Ocean. PE and PP (83 %) is the dominant type of polymer found in the surface waters. Colored particles account for 67 % of all particles, with fibre/line accounting for 86 %. The average (Mean ± SD) microplastics concentration in the Northern Indian Ocean during pre-monsoon is 15,200 ± 7999 no./km2, Monsoon is 18,223 ± 14,725 no./km2 and post monsoon is 72,381 ± 77,692 no./km2. BoB during pre-monsoon and post monsoon the microplastic concentration remains same except in the northern BoB this change is caused due to weak winds. Microplastics concentration varied both spatially, temporal and heterogeneity in nature. These differences are caused by effect of wind and seasonal reversal of currents. Microplastics collected in the anticyclonic eddy are 129,000 no./km2.


Subject(s)
Microplastics , Water Pollutants, Chemical , Plastics , Indian Ocean , Seasons , Environmental Monitoring , Water Pollutants, Chemical/analysis
12.
Mar Pollut Bull ; 186: 114405, 2023 Jan.
Article in English | MEDLINE | ID: mdl-36493514

ABSTRACT

The present study evaluates the Pan-India beach litter density (items/m2), weight (kg/m2), composition (%), and probable sources based on the citizen science approach. A total of 33 beaches in 2019 and 30 beaches in 2021 were studied. Based on density, the national beach litter average was 0.475± 0.51 and 0.3 ± 0.4 items/m2 in 2019 and 2021, respectively. Plastic was the dominant litter type (65 % in 2019; 74 % in 2021) and Single-Use Plastics (SUPs) were predominant. Based on Clean-Coast Index, six beaches in 2019 and three beaches in 2021 are classified as "extremely dirty". The principal (∼60 %) sources of litter were tourism and public littering. Improving solid waste management, stringent implementation of environmental legislation, leveraging the polluter pays principle, monitoring the effectiveness of SUPs ban, generating awareness, and beach clean-up at regular intervals by engaging the public, educational, and non-governmental organizations will improve and sustain the cleanliness of beaches.


Subject(s)
Citizen Science , Environmental Monitoring , Waste Products/analysis , Bathing Beaches , Solid Waste , Plastics
13.
PLoS One ; 17(9): e0273070, 2022.
Article in English | MEDLINE | ID: mdl-36048892

ABSTRACT

BACKGROUND: Cervical cancer is an important area of action because of the mortality and morbidity of the disease, and the potential for effective prevention by screening. Involving the social support groups by health education improves cervical cancer screening and early detection of the disease in the community. In the ongoing efforts to strengthen cervical cancer prevention, control, and management, digital health and technology will have a significant role to play. OBJECTIVE: To assess the effectiveness of the mHealth-based intervention on cervical cancer preparedness among women social support groups. MATERIALS & METHODS: A pre-post interventional study was conducted among women social support groups from lower socio-economic status, identified from the field practice area. Purposive sampling technique was employed. A Cervical Cancer Awareness Measure (CAM) instrument was used to assess the cancer preparedness among the social support group women After taking inputs from the stakeholders' mobile health application was developed. The mHealth educational intervention was given to 102 women. Both pre-and post-test questionnaires were administered through mHealth application to assess the change in knowledge after a gap of 1 month to 2 months. The data obtained was coded and entered into Microsoft Excel worksheet 2016 and was later imported and analyzed using SPSS version 22 (licensed to the Institute). The difference in median scores of knowledge and practice were interpreted as statistically significant at p value of < 0.05. RESULTS & CONCLUSION: Before the intervention only 13 (12.7%) of them had heard about cervical cancer. There was a significant increase in the knowledge about warning signs & symptoms, risk factors of cervical cancer, and HPV vaccination. Around 5% increase in Pap smear test uptake.


Subject(s)
Telemedicine , Uterine Cervical Neoplasms , Early Detection of Cancer , Female , Health Knowledge, Attitudes, Practice , Humans , Mass Screening/methods , Papanicolaou Test/methods , Social Class , Social Support , Surveys and Questionnaires , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/etiology , Uterine Cervical Neoplasms/prevention & control , Vaginal Smears
14.
Chemosphere ; 303(Pt 2): 135135, 2022 Sep.
Article in English | MEDLINE | ID: mdl-35640690

ABSTRACT

The present study investigates the abundance, distribution, and characterization (shape, size, colour, chemical composition) of microplastics (MPs) in surface water and sediment from the shelf region of the central east coast of India. The surface water and sediment samples were collected at varying depths (12.8-63 m) from 21 locations covering ∼1200 km. The mean abundance of MPs in surface water and sediments were 5.3 × 104 particles. km-2, 209 ± 99 particles. kg-1 of dry weight, respectively. Stereomicroscopy, Raman spectroscopy, and micro Fourier Transform Infra-red Spectroscopy (FTIR) were employed for the quantification and characterization of the polymers. Polyolefin (polyethylene and polypropylene) were the dominant polymers in both surface water and sediments indicating their source primarily land based. Surface water and sediment MPs were mostly blue coloured. Fibre (77%) and fragment (38%) were the dominant morphotypes in surface water and sediments, respectively. Surface characteristics studies using Scanning Electron Microscope (SEM) highlight the breakdown progress of the particles; Small MPs (<1 mm) account for >50% of the whole and dominant in the offshore region (10 km). The results reveal that the primary sources of MPs are most likely to be originating from riverine fluxes and fishing-based activities.


Subject(s)
Microplastics , Water Pollutants, Chemical , Bays , Environmental Monitoring/methods , Geologic Sediments/chemistry , Plastics/chemistry , Water , Water Pollutants, Chemical/analysis
15.
Indian J Anaesth ; 66(2): 140-145, 2022 Feb.
Article in English | MEDLINE | ID: mdl-35359484

ABSTRACT

Background and Aims: Out-of-hospital cardiac arrest is one of the leading causes of death in India. Only 1.3% of these arrests receive bystander cardiopulmonary resuscitation (CPR). Bystander CPR increases a victim's chances of survival; training school children in Hands-Only CPR (HOCPR) is a proven method of increasing bystander CPR rates. Heart Rescue India is an international project working to improve care for cardiovascular diseases, and as a part of it, a ten module Cardiovascular disease (CVD) educational programme, including HOCPR training, was conducted in ten schools in 2017-18. The objective of our study was to assess the effectiveness of HOCPR training for 8th-grade high school students. Methods: Four hundred fourteen of the 530 enroled students from ten schools of Bengaluru participated in the study. The participants attended a one-hour didactic session about the recognition of cardiac arrest and HOCPR in three simple steps. Subsequently, students received hands-on training for HOCPR. The sessions included pre- and post-assessment of knowledge and skills. The results were statistically analysed using paired t-test and the McNemar test. Results: The mean overall pre-assessment score for knowledge was 62.07 ± 28.38%, and the post-assessment score was 72.42 ± 26.58% (P < 0.001). In addition, there was a statistically significant improvement in the post-training scores for HOCPR in all three parameters, namely compressions per minute, depth and chest recoil. Conclusion: The study demonstrated a simple yet effective HOCPR programme for high school children.

16.
Mar Pollut Bull ; 176: 113424, 2022 Mar.
Article in English | MEDLINE | ID: mdl-35176547

ABSTRACT

Environmental contamination due to plastic waste mismanagement is a growing global concern. Plastic problem is of particular concern to the Indian Ocean nations as Asia currently contributes to the highest share of mismanaged plastic waste. Consequently, there is a worldwide interest to understand the distribution and transboundary movement of plastic from this region, which is crucial for implementing management measures. This review article focuses on current knowledge of plastic research, policies, waste management, socio-economics, challenges, and research opportunities. To date, marine plastic studies have focused on a few locations, providing an analysis of distribution and plastic-organism interactions in the Indian marine system. Along with scientific investigation, enforcement, improvisation, and, if necessary, framing new policies, integrated technologies to manage plastic waste, and behavioural changes are essential to mitigate plastic pollution. Such measures will be effective through a combination of actions among national and international researchers, industries, environmental managers, and the public.


Subject(s)
Plastics , Waste Management , Environmental Monitoring , Environmental Pollution , Indian Ocean , Policy , Waste Products/analysis
17.
J Midlife Health ; 13(4): 294-299, 2022.
Article in English | MEDLINE | ID: mdl-37324790

ABSTRACT

Background: In India, the elderly (aged 60 and above) constitute 8.2% of the total population and are expected to increase to 10% by the year 2020. Globally, around 450 million people are suffering from diabetes mellitus. Frailty is regarded as a predisability state and, therefore, if identified early, may avert many adverse health outcomes in the elderly. Diabetes and frailty are found to be close associates. Materials and Methods: This community-based cross-sectional study was conducted among 104 elderlies with diabetes mellitus residing in an urban slum situated in Mysuru for a period of 6 months. Pretested structured questionnaire was used to collect the information on sociodemographic characteristics and details of diabetes. The Tilburg's Frailty Scale was used to assess frailty, and the Mini Nutritional Assessment Scale was used to assess the nutritional status. Results: The prevalence of frailty among the study population was 53.8%. 51% of the subjects were found to have their glycemic status under control, 16.3% were malnourished, and 70.2% were at risk of malnutrition (RMN). The majority of the subjects with malnourishment were frail (76.5%) followed by those at RMN, 36 (49.3%). Gender, marital status, engaging in occupation, socio economic status, poor glycemic control were found to be significantly associated with frailty. Conclusion: The prevalence of frailty is significantly higher among elderly diabetics. The poorer glycemic control is a significant factor associated with frailty, and malnourished elderlies are more at risk of developing frailty.

18.
J Educ Health Promot ; 10(1): 168, 2021.
Article in English | MEDLINE | ID: mdl-34250102

ABSTRACT

BACKGROUND: Following the COVID-19 pandemic, the Government of India announced lockdown from March 25, 2020, which included measures such as social distancing, canceling mass gatherings, and closure of schools and colleges. Since the present generation of students spends a lot of time on social media, especially WhatsApp, the utility of the same to discuss the various public health domains of COVID-19 through multiple-choice questions (MCQs) was explored. MATERIALS AND METHODS: This longitudinal study was conducted among 154 participants (undergraduates, interns, postgraduates, and teaching faculty) for 3 months. MCQs on COVID-19 were disseminated through WhatsApp status of the investigator, and feedback regarding the same was taken at the end of 3 months. The activity log was maintained, and the information was downloaded as a comma-separated value (CSV) file and exported to MS Excel. Descriptive statistics were applied. The location of the participants was entered into the Microsoft Excel sheet and converted into CSV file. The Geographical data were analyzed in the Quantum Geographical information System (QGIS). RESULTS: Among the 154 study participants, 48% were undergraduates, 10.4% were interns, 30.6% were postgraduates, and 11% were teaching faculty. Eighty-nine percent of the participants felt that WhatsApp can be used as a platform for discussing MCQs. 89%, 93%, and 89.6% of the participants felt that the exercise improved their analytical skills, helped in self-directed learning, and improved collaborative learning, respectively. CONCLUSIONS: Majority of the participants responded that WhatsApp can be used as a platform to discuss MCQs and that the exercise helped in collaborative and self-directed learning.

19.
Mar Pollut Bull ; 170: 112593, 2021 Sep.
Article in English | MEDLINE | ID: mdl-34126444

ABSTRACT

In this study, we identified the in situ bacterial groups and their community structure in coastal waters influenced by anthropogenic inputs. The use of environmental DNA (eDNA) and high throughput sequencing (HTS) were employed to derive accurate and reliable information on bacterial abundance. The V3 and V4 hypervariable regions of the 16S rRNA gene were amplified and the sequences were clustered into operational taxonomic units to analyze the site-specific variations in community composition. The percentage composition within the bacterial orders varied significantly among nearshore anthropogenic hotspots and offshore (5 km) samples. The microbial network constructed taking the bacterial abundance as nodes displayed strong positive and negative correlations within the bacterial families. Overall, the use of eDNA coupled with HTS is an incredible means for monitoring and assessing the abundance of bacterial communities and also serves as a biomonitoring tool to understand the degree of anthropogenic contamination in coastal waters.


Subject(s)
DNA, Environmental , Bacteria/genetics , Catalytic Domain , DNA, Bacterial , High-Throughput Nucleotide Sequencing , Humans , RNA, Ribosomal, 16S , Seawater
20.
Mar Pollut Bull ; 169: 112468, 2021 Aug.
Article in English | MEDLINE | ID: mdl-34049072

ABSTRACT

The quantity of floating plastic debris (FPD) is continuously being increased in the oceans. To assess their size, structure, and composition along the eastern Arabian Sea (EAS), FPD samples were collected by using a surface plankton net. The microplastic size fraction (0.5-5 mm) was the most prevalent accounting for >50% of the total, followed by mesoplastics (5-25 mm; ~40%) and macroplastics (>25 mm; ~10%). The collected FPDs were categorized into five different types and eight colours. Attenuated Total Reflectance-Fourier Transform Infrared Spectrometry (ATR-FTIR) analysis of the plastics revealed that polypropylene, polyethylene, and nylon were the most dominant polymers, and these comprised mostly of fibre/fishing line. The abundance of FPD in the EAS (0.013 ± 0.012 no.s/m3) was found to be very low compared to elsewhere. The prevalent microplastics presence in the oceans might have occurred mainly by the degradation of larger items. It increases bioavailability, and hence, is a risk to marine ecosystems.


Subject(s)
Plastics , Water Pollutants, Chemical , Ecosystem , Environmental Monitoring , Indian Ocean , Water Pollutants, Chemical/analysis
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