Application of GPS occurrence data to understand African white-backed vultures Gyps africanus spatial home range overlaps.

Understanding key overlap zones and habitats which are intensively shared by species in space and time is crucial as it provides vital information to inform spatial conservation with maximum benefits. The advent of high-resolution GPS technologies associated with new analytical algorithms is revolutionizing studies underpinning species spatial and social interaction patterns within ecosystems. Here, using a robust home range estimation algorithm, the autocorrelated kernel density estimator (AKDE) equipped with an equally powerful home range overlap metric, the Bhattacharyya's coefficient (BC), we provide one of the first attempts to estimate and delineate spatial home range overlap zones for critically endangered African white-backed vultures to inform conservation planning. Six vultures were captured in Hwange National Park using a modified cannon net system after which they were tagged and tracked with high-resolution GPS backpacks. Overall, results suggested weaker average home range overlaps based on both the pooled data (0.38 ± 0.26), wet non-breeding seasonal data (0.32 ± 0.23), and dry breeding season data (0.34 ± 0.28). Vultures 4, 5, and 6 consistently revealed higher home range overlaps across all the scales with values ranging between 0.60 and 0.99. Individual vultures showed consistence in space use patterns as suggested by high between-season home range overlaps, an indication that they may be largely resident within the Hwange ecosystem. Importantly, we also demonstrate that home range overlapping geographic zones are all concentrated within the protected area of Hwange National Park. Our study provides some of the first results on African vulture home range overlaps and segregation patterns in the savanna ecosystem based on unbiased telemetry data and rigorous analytical algorithms. Such knowledge may provide vital insights for prioritizing conservation efforts of key geographic overlap zones to derive maximum conservation benefits especially when targeting wide-ranging and critically endangered African white-backed vultures. To this end, spatial overlap zones estimated here, although based on a small sample size, could provide a strong foundation upon which other downstream social and ecological questions can be explored further to expand our understanding on shared space use mechanisms among African vulture species.

Spatial distribution of Mycobacterium Tuberculosis in metropolitan Harare, Zimbabwe.

INTRODUCTION: The contribution of high tuberculosis (TB) transmission pockets in propagating area-wide transmission has not been adequately described in Zimbabwe. This study aimed to describe the presence of hotspot transmission of TB cases in Harare city from 2011 to 2012 using geospatial techniques. METHODS: Anonymised TB patient data stored in an electronic database at Harare City Health department was analysed using geospatial methods. Confirmed TB cases were mapped using geographic information system (GIS). Global Moran's I and Anselin Local Moran's I (LISA) were used to assess clustering and the local Getis-Ord Gi* was used to estimate hotspot phenomenon of TB cases in Harare City for the period between 2011 and 2012. RESULTS: A total of 12,702 TB cases were accessed and mapped on the Harare City map. In both 2011 and 2012, ninety (90%) of cases were new and had a high human immunodeficiency virus (HIV)/TB co-infection rate of 72% across all suburbs. Tuberculosis prevalence was highest in the Southern district in both 2011 and 2012. There were pockets of spatial distribution of TB prevalence across West South West, Southern, Western, South Western and Eastern health districts. TB hot spot occurrence was restricted to the West South West, parts of South Western, Western health districts. West South West district had an increased peri-urban population with inadequate social services including health facilities. These conditions were conducive for increased intensity of TB occurrence, a probable indication of high transmission especially in the presence of high HIV co-infection. CONCLUSIONS AND RECOMMENDATIONS: Increased TB transmission was limited to a health district with high informal internal migrants with limited health services in Harare City. To minimise spread of TB into greater Harare, there is need to improve access to TB services in the peri-urban areas.

Spatial overlaps in the distribution of HIV/AIDS and malaria in Zimbabwe.

BACKGROUND: In most developing economies particularly in Africa, more people are likely to die of HIV/AIDS and malaria compared to other diseases. HIV/AIDS tends to be superimposed on the long standing malaria burden particularly in sub-Saharan Africa. The detection and understanding of spatial overlaps in disease occurrence is important for integrated and targeted disease control. Integrated disease control can enhance efficiency and cost-effectiveness through the development of drugs targeting multiple infections in the same geographic space. METHODS: Using Zimbabwe as a case study, this study tests the hypothesis that malaria clusters coincide with HIV/AIDS clusters in space. Case data for the two diseases were obtained from the Ministry of Health and Child Care in Zimbabwe at district level via the District Health Information System (DHIS). Kulldorff's spatial scan statistic was used to test for spatial overlaps in clusters of high cases of HIV/AIDS and malaria at district level. The spatial scan test was used to identify areas with higher cases of HIV/AIDS and malaria than would be expected under spatial randomness. RESULTS: Results of this study indicate that primary clusters of HIV/AIDS and malaria were not spatially coincident in Zimbabwe. While no spatial overlaps were detected between primary clusters of the two diseases, spatial overlaps were detected among statistically significant secondary clusters of HIV/AIDS and malaria. Spatial overlaps between HIV/AIDS and malaria occurred in five districts in the northern and eastern regions of Zimbabwe. In addition, findings of this study indicate that HIV/AIDS is more widespread in Zimbabwe compared to malaria. CONCLUSIONS: The results of this study may therefore be used as a basis for spatially-targeted control of HIV/AIDS and malaria particularly in high disease burden areas. This is important as previous interventions have targeted the two diseases separately. Thus, targeted control could assist in resource allocation through prioritising areas in greatest need hence maximising the impact of disease control.

Antibacterial activity of aqueous and methanol extracts of selected species used in livestock health management.

CONTEXT: Salvadora persica L. (Salvadoraceae), Colophospermum mopane (J.Kirk ex Benth.) J. Léonard (Leguminosae) and Dichrostachys cinerea (L.) Wight & Arn. (Leguminosae) crude extracts are used by local farmers against many livestock infections with little or no side effects usually associated with synthetic antimicrobials. However, their efficacy has rarely been tested. OBJECTIVE: These plants were tested for potential antibacterial activity against clinical isolates of Staphylococcus aureus ATCC33862 and Escherichia coli ATCC25922. Minimal inhibitory concentrations (MIC) of the crude plant extracts were determined. MATERIALS AND METHODS: Aqueous and methanol extraction of 100 g each of the bark of C. mopane, roots of D. cinerea and leaves of S. persica was done by placing the samples in 250 mL of either water or methanol. Nutrient broth was used as growth medium for the bacteria, and McFarland standard for bacterial standardization. 2,3,5-Triphenyltetrazoliumchloride (TTC) was the indicator salt. Each of the aqueous and methanol extracts (100 µL) was tested. Gentamycin and ampicillin were the controls. RESULTS: MIC of aqueous extracts ranged from 1.03-14.6 mg/mL against S. aureus, and from 12.1-34.3 mg/mL against E. coli. Methanol extracts ranged between 5.31 and 9.64 mg/mL against S. aureus, and between 7.86 and 13.6 mg/mL against E. coli. Aqueous and methanol extracts of S. persica were significantly higher (p < 0.05) than C. mopane and D. cinerea. DISCUSSION AND CONCLUSION: Colophospermum mopane, S. persica and D. cinerea exhibited antibacterial activity, with methanol extracts performing better than aqueous extracts, justifying use as ethnoveterinary medicine. Further study to isolate the active components should be pursued.