Dynorphin administered to newborn rats modulates morphogenesis of the heart.

Proliferation, activity of the nucleolar organizer region, and protein content in cardiomyocytes were studied in 21-day-old rats intraperitoneally treated with kappa-opioid receptor agonist dynorphin A(1-13) in a dose of 100 microg/kg on postnatal days 2-6. The content of catecholamines in the heart and proliferative activity of cardiomyocytes remained unaffected, but the number of nucleoli in cardiomyocyte nuclei and the integral optical density of cardiomyocytes stained with amido black B (protein content) increased. The data suggest that administration of dynorphin to newborn rats considerably modulates morphogenesis of the heart at later terms.

Effect of mu-opiate receptor agonist tetrapeptide A10 on DNA synthesis and protein content in the myocardium of albino rats.

Effect of intraperitoneal injection of tetrapeptide A10 (H-Tyr-D-Orn-Phe-Gly-OH), selective mu-opiate receptor agonist, synthetic analog of dermorphine, in a dose of 100 microg/kg on DNA synthesis and protein content in the myocardium was studied in albino rats. Five injections of tetrapeptide on days 2-6 after birth caused no changes in DNA synthesis 17 days after the last injection, i. e. in 24-day rats. The number of nucleoli and their area increased. In adult males long-term (3-week) treatment with tetrapeptide A10 increased the number of nucleoli and the mean and integral optical density of isolated cardiomyocytes stained with amido black B, which probably attested to activation of protein synthesis in the myocardium. Simultaneously, the content of catecholamines in the heart increased. These data are comparable with delayed effects of kappa-opiate receptor agonist dinorphine A1-13 and indicate that morphogenetic properties of opioid peptides in rat myocardium are realized via the same routes.

[Morphofunctional features of the ovary remaining after unilateral ovariectomy in various stages of the sexual cycle and during pregnancy]. / Morfofunktsional'nye osobennosti iaichnika, ostavshegosia posle odnostoronnei ovariéktomii, v raznye fazy polovogo tsikla i pri beremennosti.

The activity of 3 beta-ol-steroid-dehydrogenase in yellow bodies, theca, athretic bodies of hemi-ovariectomized rats and the mass of steroid-producing ovary structure during diestrus, estrus on the 8th, 20th days of pregnancy was studied. The findings about progesterone concentration, number and mass of fetus and placenta in hemi-ovariectomized rats as those in control are presented.

[Effects of atrial natriuretic peptide AP II on the morphofunctional state of the adrenal cortex of white rats]. / Vliianie predserdnogo natriiureticheskogo peptida AP II na morfofunktsional'noe sostoianie kory nadpochechnikov belykh krys.

The effect of atrial natriuretic peptides synthetic analog AP II on adrenal zona glomerulosa and zona fasciculata physiological regeneration have been studied on male rats. The 3H-thymidine incorporation into DNA in adrenal cortical cells was evaluated in 4 and 24 h after intraperitoneal injection of 10 or 100 mcg/kg AP II. Besides, we have investigated the influence of AP II on adrenal cortical cells karyometric parameter in 4 and 24 h and aldosterone plasma concentration in 1 h after injection. 10 mcg/kg AP II increased the fraction of labelled nuclei in zona glomerulosa and decreased the aldosterone plasma level. No significant changes were seen in zona fasciculata cells proliferation. 100 mcg/kg AP II inhibited the DNA synthesis process in adrenal zona fasciculata, but had no significant influence on zona glomerulosa physiological regeneration and aldosterone plasma concentration. No nuclear morphometric parameter changes were observed in adrenal glomerulosa and fasciculata cells of AP II--treated animals.

[The effect of hydra peptide morphogen on the levels of beta-endorphin and some blood and adrenal hormones in albino rats]. / Vliianie peptidnogo morfogena gidry na uroben' beta-éndorfina i nekotorykh gormonov krovi i nadpochechnikov belykh krys.

The influence of PMH on the level of beta-endorphin and some hormones of blood and adrenal glands was studied. The dose A (10 mkg/kg) and dose W (100 mkg/kg) of PMH were used in our experiments. Earlier it has been discovered, that PMH in such doses stimulated the processes of cell division in 24 hours since the moment of injection. The stimulation was dose-dependent. Within 24 hours PMH in A dose decreased the concentration of beta-endorphin in the blood 2.7-fold, ad in dose W increased it 2 times. The level of corticosterone in blood and adrenal glands after the injection of PMH in dose A exceeded the control data trustworthy in 4 and 24 hours since the moment of injection. In dose B in 4 hours 1.5-fold reduction of corticosterone concentration took place in the blood. Increase in epinephrine level in adrenal glands was observed after PMH administration in two doses. Content of T3 increased in 4 hours after PMH injection in dose B. The role of hormonal changes in stimulating cell division accompanied by PMH injection is discussed. The data received show that PMH influences directly proliferative processes.

[Effects of dalargin on the processes of cell division in the gastric epithelium during stress]. / Vliianie dalargina na protsessy razmnozheniia kletok v épitelii zheludka pri stresse.

Influence of dalargine on cell division in stomach epithelium of male rats, stressed by 4-hour immobilization was studied using autoradiographic 3H-thymidine test. Norepinephrine and diene conjugates content in stomach tissue were also determined in these animals spectrofluorometrically. Dalargine (10 micrograms/kg) gas injected to experimental group 40 min before stressing. Dalargine prevented stress-induced DNA-synthesis disturbances and stabilized proliferating cells pool just after the stress. It also decreased proliferation processes depression in 12 and 24 hours after stress and accelerated compensatory DNA-synthesis.

[Morphometric analysis of the reversibility of adrenal cortex changes in rabbits following cholesterol withdrawal]. / Morfometricheskii analiz obratimosti izmenenii kory nadpochechnikov krolikov posle otmeny vvedeniia kholesterina.

The morphometric, cytophotometric and biochemical methods were used to study the reversibility of changes in the adrenal cortex of rabbits given cholesterol for 14 days. The majority of changes that develop in experimental hypercholesterolemia were discovered to undergo regression 1.5 months after cholesterol withdrawal.

[Effect of traumatic shock on the cytogenetic processes in the epithelial cells of the cornea and tongue and in the bone marrow cells of white rats]. / Vliianie tramaticheskogo shoka na tsitogeneticheskie protsessy v kletkakh épiteliia rogovitsy i iazyka i v kletkakh kostnogo mozga belykh krys.

Traumatic shock in male albino rats as induced by prolonged (4-hour) compression of soft tissues of the hind limbs. It was found that 3, 6 and 24 hours after the termination of compression the mitotic activity in the corneal epithelium decreased 9.8-, 4.7- and 3-fold, respectively. The level of pathological mitoses in experimental animals rose 2.4 - 3-fold. Experiments in colchicine demonstrated that inhibition of mitotic activity is not related to the changed rate of mitosis. In the tongue epithelium, the depression of cell division was revealed 3 and 6 hours after compression was terminated. The mitotic index fell 2.7- and 3.7-fold, respectively. Radioautography showed a significant decrease in DNA-synthetizing capacity of epithelial cells of the tongue 3 and 6 hours after the compression was terminated. Karyotypic analysis of bone marrow cells showed that chromosome aberrations increased 3.3-fold.

[Effect of repeated sublethal overheating on the cytogenetic processes in the corneal epithelium and bone marrow cells of white rats]. / Vliianie mnogokratnogo subletal'nogo peregravaniia na tsitogeneticheskie protsessy v épitelii rogovitsy i kletkakh kostnogo mozga belykh krys.

A study was made of the effect of five-fold 1 1/2-hour sublethal hyperthermia on cytogenetic processes in the corneal epithelium and bone marrow cells in rats. Hyperthermia exerted a powerful stressful effect on the animals. Immediately after hyperthermia was terminated the mitotic activity (MA) was inhibited almost 10-fold. After 2 hours the MA returned to normal and tended to increase, and after 6 hours it increased almost 5-fold. The level of pathological mitoses (PM) increased 10-fold after overheating and exceeded 2-4-fold the control values at varying times of the observation period. After 2 and 6 hours five-fold sublethal hyperthermia was accompanied by the decrease in the number of DNA-synthesizing cells and in the label intensity. At the same time hyperthermia in adrenalectomized animals brought about the reduction of the colchicine mitotic index after 2 hours. The content of aneuploidy cells in adrenalectomized animals exposed to five-fold hyperthermia increased 6-fold, whereas in the animals with the adrenals it remained unchanged.

[Morphofunctional findings on the reversibility of changes occurring during cold stress]. / Morfofunktsional'nye dannye ob obratimosti izmenenii, voznikaiushchikh pri khronicheskom kholodovom stresses.

White rats exposed to low temperature (5-7 degrees C) over 80 days showed a slow growth of the body weight and liver, lowering of liver glycogen and cholesterol content, increase in the relative weight of the thyroid gland and in the nuclei size of adrenal medulla, fall of blood 11-HOCS level, change in the mitotic phase ratio in the epithelium of the tongue and cornea, and an increase in the number of pathological mitoses in the cornea. A great number of changes did not appear fully reversible after the discontinuation of the exposure to low temperatures.

[Effect of repeated cooling on the state of the adrenals and corneal epithelial proliferation in white rats]. / Vliianie mnogokratnogo okhlazhdeniia na sostoianie nadpochechnikov i proliferatsiiu épiteliia reogovitsy belykh krys.

The effect of repeated cooling on the adrenal glands, on the mitosis and the number of DNA-synthesising cells in the corneal epithelium of albino rats was studied. The animals were cooled by the contact method to 28 degrees C and were exposed to this temperature for an hour daily within 5 days. Strong activation of the suprarenal glands was observed: 2-fold increase in the suprarenal weight, 3-fold decrease in cholesterol content, 4-fold growth of 11-hydroxy-corticosteroid level in the blood and enhanced adrenaline excretion. The average number of mitoses in the corneal decreases 2-fold. Depression was not associated with the change in mitosis rate but with the inhibition of the cell entrance into mitosis in interphase. The level of pathological mitoses did not change. Chronic stress was not accompanied by the change in the number of DNA-synthesising cells or intensity of DNA-symthesis.

[Effect of chronic pyrogenal stress on the mitotic regime and quantity of DNA-synthesizing cells in the epithelium of the cornea and tongue of white rats]. / Vliianie khronicheskogo pirogenalovogo stressa na mitoticheskii rezhim i kolichestvo DNK-sinteziruiushchikh kletok v epitelii rogovitsy i iazyka u belykh krys.

The effect of prolonged stress on mitotic regimen and the number of DNA-synthesizing cells in the corneal and tongue epithelium was studied on a model of pyrogenal stress. A five-day pyrogenal administration led to a 1.7-fold reduction of te number of mitoses in the corneal and tongue epithelium. Reduction in number of dividing cells was not associated with the change in the rate of mitosis. The percentage of pathological mitoses in the corneal epithelium of intact rats was 6.1, and it failed to change in stress. In control rats the labeled nuclei index in the corneal and tongue epithelium constituted 12.6 and 10.8. It was similar with the corresponding indices in experimental animals (12.2 and 12.2).