Instruction alters the influence of allocentric landmarks in a reach task.

Allocentric landmarks have an implicit influence on aiming movements, but it is not clear how an explicit instruction (to aim relative to a landmark) influences reach accuracy and precision. Here, 12 participants performed a task with two instruction conditions (egocentric vs. allocentric) but with similar sensory and motor conditions. Participants fixated gaze near the center of a display aligned with their right shoulder while a target stimulus briefly appeared alongside a visual landmark in one visual field. After a brief mask/memory delay the landmark then reappeared at a different location (same or opposite visual field), creating an ego/allocentric conflict. In the egocentric condition, participants were instructed to ignore the landmark and point toward the remembered location of the target. In the allocentric condition, participants were instructed to remember the initial target location relative to the landmark and then reach relative to the shifted landmark (same or opposite visual field). To equalize motor execution between tasks, participants were instructed to anti-point (point to the visual field opposite to the remembered target) on 50% of the egocentric trials. Participants were more accurate and precise and quicker to react in the allocentric condition, especially when pointing to the opposite field. We also observed a visual field effect, where performance was worse overall in the right visual field. These results suggest that, when egocentric and allocentric cues conflict, explicit use of the visual landmark provides better reach performance than reliance on noisy egocentric signals. Such instructions might aid rehabilitation when the egocentric system is compromised by disease or injury.

A role for dopamine in control of the hypoxic ventilatory response via D2 receptors in the zebrafish gill.

Dopamine is a neurotransmitter involved in oxygen sensing and control of reflex hyperventilation. In aquatic vertebrates, oxygen sensing occurs in the gills via chemoreceptive neuroepithelial cells (NECs), but a mechanism for dopamine in autonomic control of ventilation has not been defined. We used immunohistochemistry and confocal microscopy to map the distribution of tyrosine hydroxylase (TH), an enzyme necessary for dopamine synthesis, in the gills of zebrafish. TH was found in nerve fibers of the gill filaments and respiratory lamellae. We further identified dopamine active transporter (dat) and vesicular monoamine transporter (vmat2) expression in neurons of the gill filaments using transgenic lines. Moreover, TH- and dat-positive nerve fibers innervated NECs. In chemical screening assays, domperidone, a D2 receptor antagonist, increased ventilation frequency in zebrafish larvae in a dose-dependent manner. When larvae were confronted with acute hypoxia, the D2 agonist, quinpirole, abolished the hyperventilatory response. Quantitative polymerase chain reaction confirmed expression of drd2a and drd2b (genes encoding D2 receptors) in the gills, and their relative abundance decreased following acclimation to hypoxia for 48 h. We localized D2 receptor immunoreactivity to NECs in the efferent gill filament epithelium, and a novel cell type in the afferent filament epithelium. We provide evidence for the synthesis and storage of dopamine by sensory nerve terminals that innervate NECs. We further suggest that D2 receptors on presynaptic NECs provide a feedback mechanism that attenuates the chemoreceptor response to hypoxia. Our studies suggest that a fundamental, modulatory role for dopamine in oxygen sensing arose early in vertebrate evolution.