Effect of Raphanus raphanistrum on chronic kidney disease induced by ethanol in animal model rats / Efeito do Raphanus raphanistrum na doença renal crônica induzida por etanol em ratos modelo animal

The aim of this study is to investigate the effect of Raphanus raphanistrum (radish) on chronic kidney disease damage by reactive oxygen species or free radicals in animal model rats. Total of 18 rats were used in this study, divided into 3 groups and each group consist of 6 rats. Group 1 control (C), group 2 model (M) and group 3 test (T). Model and test group were treated with alcohol to produce chronic kidney disease by reactive oxygen spices for 9 weeks a dose of 1 ml. After that test group was treated with Raphanus raphanistrum juice for 4 weeks 80mg/kg body weight to determine it effect. Raphanus raphanistrum juice effect on behavior of rats through increases the locomotor activity and anxiety. The serum creatinine and uric acid level were significantly improved in T group. The reactive oxygen enzyme test shows that Super Oxide Dismutase (SOD) and Glutathione Peroxidase (GPx) was increase in T group. The Glutathione S-Transferases (GST) and Catalase (CAT) enzyme level was nearly same in C and T groups. This study concludes that compound 1,1-diphenyl-2-picrylhydrazyl found in Raphanus raphanistrum juice and possess strong antioxidant activity on Chronic kidney disease induce by ethanol through reactive oxygen species. There is need of more researches to determine the use of natural compound to treat different disease.

O objetivo deste estudo é investigar o efeito de Raphanus raphanistrum (rabanete) nos danos da doença renal crônica por espécies reativas de oxigênio ou radicais livres em ratos modelo animal. Um total de 18 taxas foi utilizado neste estudo, divididas em 3 grupos e cada grupo consistia em 6 ratos. Grupo 1 controle (C), grupo 2 modelo (M) e grupo 3 teste (T). O modelo e o grupo de teste foram tratados com álcool para produzir doença renal crônica por meio de especiarias reativas de oxigênio por 9 semanas, uma dose de 1 ml. Após esse teste, o grupo foi tratado com suco de Raphanus raphanistrum por 4 semanas 80 mg / kg de peso corporal para determinar seu efeito. Efeito do suco de Raphanus raphanistrum no comportamento de ratos por meio do aumento da atividade locomotora e ansiedade. A creatinina sérica e o nível de ácido úrico melhoraram significativamente no grupo T. O teste da enzima oxigênio reativa mostra que a Super Óxido Dismutase (SOD) e a Glutationa Peroxidase (GPx) aumentaram no grupo T. O nível das enzimas Glutationa S-Transferases (GST) e Catalase (CAT) foi quase o mesmo nos grupos C e T. Este estudo conclui que o composto 1,1-difenil-2-picrilhidrazil encontrado no suco de Raphanus raphanistrum possui forte atividade antioxidante na doença renal crônica induzida pelo etanol por meio de espécies reativas de oxigênio. Há necessidade de mais pesquisas para determinar o uso de compostos naturais no tratamento de diferentes doenças.

Assessment of heavy metals in cyprinid fishes: Rivers of district Khuzdar Balochistan Pakistan / Avaliação de metais pesados em peixes ciprinídeos: Rios do distrito de Khuzdar Balochistan, Paquistão

The present study was conducted to measured heavy metals in cyprinid fishes in rivers of District Khuzdar Balochistan, Pakistan. In the present study, 25 fish samples were collected that belonged to 8 order of 13 families, The Cyprinidae family had the largest number of eight fish species. Present study is focused on Heavy metals in cyprinid fishes. Heavy metals accumulation like Zinc, Manganese, Copper, and Nickel was evaluated in water and various organs of fishes. Atomic Absorption Spectroscopy was used for the identification of these heavy metals in fish species and water bodies. The average concentration (mg/L) of Zn 0.26-0.41, Mn 0.030- 0.073, Cu 0.017—0.080 and NI 0.14-0.79 were observed in water. The Concentration (mg/L), of Zn Conc 0.383-.028 Mn Conc .073- .030 Cu Conc 080-.017 NI Conc .79-.14. The concentration of heavy metals was found both similar and varied simultaneously across the whole research area. Zinc concentration was reported highest, whereas Copper was at the lowest concentration in all fish species .The concentration of heavy metals, in all the fish species under this study, was above the threshold of WHO limits.

O presente estudo foi realizado para medir metais pesados em peixes ciprinídeos em rios do Distrito Khuzdar Balochistan, Paquistão. No presente estudo, foram coletadas 25 amostras de peixes pertencentes a 8 ordens de 13 famílias. A família Cyprinidae apresentou o maior número de oito espécies de peixes. O presente estudo está focado em metais pesados em peixes ciprinídeos. O acúmulo de metais pesados como zinco, manganês, cobre e níquel foi avaliado na água e em vários órgãos dos peixes. A Espectroscopia de Absorção Atômica foi utilizada para a identificação desses metais pesados em espécies de peixes e corpos d'água. A concentração em água ((mg/L),) Zn Conc. 0,383-.028 Mn Conc. .073- .030 Cu Conc. 080-.017 NI Conc. 0,79-.14. A concentração de metais pesados foi considerada semelhante e variou simultaneamente em toda a área de pesquisa. A concentração de zinco foi relatada mais alta, enquanto o cobre estava na concentração mais baixa em todas as espécies de peixes. A concentração de metais pesados, em todas as espécies de peixes neste estudo, estava acima do limite dos limites da Organização Mundial da Saúde (OMS).

Antioxidant associated chemoprophylaxis effect of natural spice and green vegetable on hepatotoxicity / Efeito de quimioprofilaxia na hepatotoxicidade associada a antioxidantes de especiarias naturais e vegetais verdes

This is a case control and cohort study, conducted at Department of Biochemistry in FUAST, compressing 24 number of albino Wistar rats. Illicium verum (star anise) natural spice and green vegetable broccoli are used for chemo-preventive herbal treatment in relation with vitamin C antioxidant activity. Hepatotoxicity was induced in liver of model rats by giving interperitoneally single injection of 200 mg/kg bod weight (B.W) of N-nitrosodiethylamine and orally giving 1 mg/kg BW phenobarbital for 14 days during disease prorogation period. The vitamin C analysis from Illicium verum (star anise), green broccoli, orange juice, orange peel and orange pulp was done by iodometric quantitative and qualitative titration method. Rats were given compound according to their bodyweight as 1 mg/kg B.W. After 14 days (Disease Promotion and Propagation Period) and 28 days (Disease cure period) of treatment behavioral activity was monitored by locomotors activity in open field experiment, stimulatory activity in home cage and anxiolytic effects observed in light and dark apparatus and also in T maze. Behavioral activity were significantly increased in Illicium verum and green broccoli treated rats. Hematological study including hemoglobin (Hb), white blood cells (WBC), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW) and Platelets was done by abbot laboratory analyzer cell. The value of Hb and RBC was also significantly increased in star anise and broccoli treated rats, showed proper function of RBC in microcirculation. WBC was also in normal range revealed that no disease regards to bone marrow and leukemia's. The biochemical activity of rats including urea, creatinine, total protein, albumin, globulin, A/G ratio, amylase and calcium in blood serum were analyzed while, liver health or performance was also determined by LFT (liver function test). Behavioral and laboratorial variables were analyzed by using SPSS v. 20 and p-value < 0.05 was considered statistically significant. The study report noticeable significant effect of Illicium verum and broccoli on hepatotoxicity of experimental model. Recent experimental study statistical outcomes show reciprocal relationship between the consumption of vegetable diet and natural spice with the risk of Hepatotoxicity. Thus, the aim of the study is to enhance the function of star anise or illicium verum natural spice and green vegetable broccoli as an anticancer or chemoprophylaxis agent.

Trata-se de um estudo de caso controle e coorte, realizado no Departamento de Bioquímica da FUAST, comprimindo 24 ratos albinos Wistar. A especiaria natural Illicium verum (anis estrelado) e o brócolis vegetal verde geralmente são utilizados ​​para o tratamento fitoterápico quimiopreventivo por conta da atividade antioxidante da vitamina C. A hepatotoxicidade foi induzida no fígado dos ratos modelo por injeção única interperitoneal de 200 mg/kg de peso corporal (PC) de N-nitrosodietilamina e administração oral de 1 mg/kg de peso corporal de fenobarbital por 14 dias durante o período de prolongamento da doença. A análise da vitamina C de Illicium verum (anis estrelado), brócolis verde, suco de laranja, casca de laranja e polpa de laranja foi realizada pelo método de titulação quantitativa e qualitativa iodométrica. Os ratos receberam composto de acordo com seu peso corporal como 1 mg/kg B.W. Após 14 dias (Período de Promoção e Propagação da Doença) e 28 dias (Período de Cura da Doença) de tratamento a atividade comportamental foi monitorada por atividade locomotora em experimento de campo aberto, atividade estimulatória em gaiola e efeitos ansiolíticos observados em aparelhos claros e escuros e também em T Labirinto. A atividade comportamental foi significativamente aumentada em ratos tratados com Illicium verum e brócolis verde. O estudo hematológico incluindo hemoglobina (Hb), glóbulos brancos (WBC), glóbulos vermelhos (RBC), volume corpuscular médio (MCV), hemoglobina corpuscular média (MCH), concentração média de hemoglobina corpuscular (MCHC), largura de distribuição de glóbulos vermelhos (RDW) e Plaquetas foi feito pela célula do analisador de laboratório Ababot. O valor de Hb e RBC também foi significativamente aumentado em ratos tratados com anis estrelado e brócolis, mostraram função adequada de RBC na microcirculação. WBC também estava na faixa normal revelando que nenhuma doença relaciona-se à medula óssea e leucemia. A atividade bioquímica de ratos, incluindo uréia, creatinina, proteína total, albumina, globulina, razão A/G, amilase e cálcio no soro sanguíneo foram analisadas, enquanto a saúde ou desempenho do fígado também foi determinada por LFT (teste de função hepática). As variáveis ​​comportamentais e laboratoriais foram analisadas pelo SPSS v. 20 e valor de p < 0,05 foi considerado estatisticamente significativo. O estudo relata efeito significativo perceptível de Illicium verum e brócolis na hepatotoxicidade do modelo experimental. Resultados estatísticos de estudos experimentais recentes mostram relação recíproca entre o consumo de dieta vegetal e tempero natural com o risco de hepatotoxicidade. Assim, o objetivo do estudo foi potencializar a função do anis estrelado ou da especiaria natural Illicium verum e do brócolis vegetal verde como agente anticancerígeno ou quimioprofilático.

Antioxidant associated chemoprophylaxis effect of natural spice and green vegetable on hepatotoxicity.

This is a case control and cohort study, conducted at Department of Biochemistry in FUAST, compressing 24 number of albino Wistar rats. Illicium verum (star anise) natural spice and green vegetable broccoli are used for chemo-preventive herbal treatment in relation with vitamin C antioxidant activity. Hepatotoxicity was induced in liver of model rats by giving interperitoneally single injection of 200 mg/kg bod weight (B.W) of N-nitrosodiethylamine and orally giving 1 mg/kg BW phenobarbital for 14 days during disease prorogation period. The vitamin C analysis from Illicium verum (star anise), green broccoli, orange juice, orange peel and orange pulp was done by iodometric quantitative and qualitative titration method. Rats were given compound according to their bodyweight as 1 mg/kg B.W. After 14 days (Disease Promotion and Propagation Period) and 28 days (Disease cure period) of treatment behavioral activity was monitored by locomotors activity in open field experiment, stimulatory activity in home cage and anxiolytic effects observed in light and dark apparatus and also in T maze. Behavioral activity were significantly increased in Illicium verum and green broccoli treated rats. Hematological study including hemoglobin (Hb), white blood cells (WBC), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW) and Platelets was done by abbot laboratory analyzer cell. The value of Hb and RBC was also significantly increased in star anise and broccoli treated rats, showed proper function of RBC in microcirculation. WBC was also in normal range revealed that no disease regards to bone marrow and leukemia's. The biochemical activity of rats including urea, creatinine, total protein, albumin, globulin, A/G ratio, amylase and calcium in blood serum were analyzed while, liver health or performance was also determined by LFT (liver function test). Behavioral and laboratorial variables were analyzed by using SPSS v. 20 and p-value < 0.05 was considered statistically significant. The study report noticeable significant effect of Illicium verum and broccoli on hepatotoxicity of experimental model. Recent experimental study statistical outcomes show reciprocal relationship between the consumption of vegetable diet and natural spice with the risk of Hepatotoxicity. Thus, the aim of the study is to enhance the function of star anise or illicium verum natural spice and green vegetable broccoli as an anticancer or chemoprophylaxis agent.

Assessment of heavy metals in cyprinid fishes: Rivers of district Khuzdar Balochistan Pakistan.

The present study was conducted to measured heavy metals in cyprinid fishes in rivers of District Khuzdar Balochistan, Pakistan. In the present study, 25 fish samples were collected that belonged to 8 order of 13 families, The Cyprinidae family had the largest number of eight fish species. Present study is focused on Heavy metals in cyprinid fishes. Heavy metals accumulation like Zinc, Manganese, Copper, and Nickel was evaluated in water and various organs of fishes. Atomic Absorption Spectroscopy was used for the identification of these heavy metals in fish species and water bodies. The average concentration (mg/L) of Zn 0.26-0.41, Mn 0.030- 0.073, Cu 0.017-0.080 and NI 0.14-0.79 were observed in water. The Concentration (mg/L), of Zn Conc 0.383-.028 Mn Conc .073- .030 Cu Conc 080-.017 NI Conc .79-.14. The concentration of heavy metals was found both similar and varied simultaneously across the whole research area. Zinc concentration was reported highest, whereas Copper was at the lowest concentration in all fish species .The concentration of heavy metals, in all the fish species under this study, was above the threshold of WHO limits.