Carbamoyl Phosphate Synthase Subunit CgCPS1 Is Necessary for Virulence and to Regulate Stress Tolerance in Colletotrichum gloeosporioides.

Glomerella leaf spot (GLS) is a severe infectious disease of apple whose infective area is growing gradually and thus poses a huge economic threat to the world. Different species of Colletotrichum including Colletotrichum gloeosporioides are responsible for GLS. For efficient GLS control, it is important to understand the mechanism by which the cruciferous crops and C. gloeosporioides interact. Arginine is among one of the several types of amino acids, which plays crucial role in biochemical and physiological functions of fungi. The arginine biosynthesis pathway involved in virulence among plant pathogenic fungi is poorly understood. In this study, CgCPS1 gene encoding carbamoyl phosphate synthase involved in arginine biosynthesis has been identified and inactivated experimentally. To assess the effects of CgCPS1, we knocked out CgCPS1 in C. gloeosporioides and evaluated its effects on virulence and stress tolerance. The results showed that deletion of CgCPS1 resulted in loss of pathogenicity. The Δcgcps1 mutants showed slow growth rate, defects in appressorium formation and failed to develop lesions on apple leaves and fruits leading to loss of virulence while complementation strain (CgCPS1-C) fully restored its pathogenicity. Furthermore, mutant strains showed extreme sensitivity to high osmotic stress displaying that CgCPS1 plays a vital role in stress response. These findings suggest that CgCPS1 is major factor that mediates pathogenicity in C. gloeosporioides by encoding carbamoyl phosphate that is involved in arginine biosynthesis and conferring virulence in C. gloeosporioides.

Molecularly imprinted polymers' application in pesticide residue detection.

Molecularly imprinted polymers (MIPs) are produced using molecular imprinting technology (MIT) and have specific analyte-binding abilities and unique properties, including chemical and thermal stability, reusability, high selectivity, and high sensitivity. The application of MIPs in the detection of pesticides represents an advance and a superior scientific approach owing to their detection and characterization of trace levels in comparison with other methods. In this review, we have summarized the pre-treatment extraction of pesticides with different types of molecularly imprinted polymer for the detection of single and multiple pesticides by elaborating upon their specific extraction efficiency. The importance of different polymerization methods, functional monomers and cross-linkers is highlighted. The aim of this study is to investigate the importance of the application of MIPs in the detection of pesticides and recent advances in the last few years to overcome the limitations of previously developed methods. Existing restrictions and required future aspects are discussed.