1.
J Biochem Biophys Methods
; 20(1): 53-68, 1989.
Article
in English
| MEDLINE
| ID: mdl-2630586
ABSTRACT
Peptide: N-glycosidase from Flavobacterium meningosepticum was isolated in a homogeneous state and its physico-chemical characterization was accomplished. The reliability of the previously recorded assay procedures was assessed. Using an octaglycopeptide derived from ovomucoid a rapid and sensitive FPLC method was developed for the assay of enzymatic activity. Peptide: N-glycosidase was found to effect deglycosylation of glycoproteins bearing complex and/or multiantennary glycans even in their native state. In contrast, glycoproteins with high mannose and/or hybrid carbohydrates required denaturation to become susceptible to deglycosylation by the enzyme.
Subject(s)
Amidohydrolases/metabolism , Flavobacterium/enzymology , Glycoproteins/metabolism , Amino Acids/analysis , Carbohydrates/analysis , Chromatography, High Pressure Liquid , Glycoproteins/analysis , Molecular Weight , Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase , Substrate Specificity
2.
J Chromatogr
; 408: 378-84, 1987 Nov 06.
Article
in English
| MEDLINE
| ID: mdl-3429535