ABSTRACT
Background: Infectious diseases of young and adult birds with respiratory syndrome are a significant deterrent to the development of industrial poultry farming due to decreased productivity and significant mortality. The only effective method of combating viral diseases is timely and targeted vaccination, which largely depends on laboratory diagnostic results. Aim: This article aims to study the real-time reverse transcription polymerase chain reaction, (RT-PCR) which has the prospect of more effective diagnosis of vaccine strains of chicken infectious bronchitis and Newcastle disease. Methods: The fastest and most accurate method for the differential diagnosis of pathogens in an associative viral infection is RT-PCR. The method proposed in the article for selecting primers for amplification made it possible to use this method for the simultaneous interspecies differential diagnosis of two or more viral agents, significantly accelerating their diagnosis. Results: The correlation of the nucleotide sequence obtained from sequencing to a specific virus strain is complicated by the lack of a single nomenclature mechanism for separating genetic groups. Conclusion: The results of this study will allow easy and fast typing of sequences into known and databased virus strains and avoid further confusion in the nomenclature of genetic groups in the future.
Subject(s)
Bronchitis , Communicable Diseases , Coronavirus Infections , Newcastle Disease , Poultry Diseases , Vaccines , Animals , Chickens , Newcastle Disease/diagnosis , Newcastle Disease/prevention & control , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Poultry Diseases/diagnosis , Poultry Diseases/prevention & control , Coronavirus Infections/diagnosis , Coronavirus Infections/prevention & control , Coronavirus Infections/veterinary , Communicable Diseases/veterinary , Bronchitis/veterinaryABSTRACT
Background: The relevance of the study is determined by the danger that this disease poses to all types of domestic animals in the Republic of Kazakhstan, as well as the significant damage caused by this situation to the entire agriculture of the Republic of Kazakhstan. Timely and competent analysis of the epizootic situation for this disease in the country will reliably determine the development tendencies of this condition and predetermine a set of measures that should be taken to combat this animal disease in agricultural regions of the Republic of Kazakhstan. Aim: This study examines the development of the epizootic situation of Aujeszky's disease within the territory of the Republic of Kazakhstan. Methods: To achieve the set research goal, a combination of quantitative and qualitative research methods are used. Results: The results of this research article can be of significant importance for livestock breeders in the Republic of Kazakhstan, who are working on raising the number of pigs and other types of domestic animals and are interested in a further increase in the number. Conclusion: From a practical standpoint, the significance of this study is conditioned by the possibility of searching and determining the main directions of the spread of the disease under consideration in agricultural lands and regions of the Republic of Kazakhstan, as well as taking a set of measures to eradicate such a disease and prevent its future occurrence in the country.
