Subject(s)
Cell Biology/standards , Early Detection of Cancer/standards , Laboratories/standards , Mass Screening/standards , Practice Guidelines as Topic , Uterine Cervical Neoplasms/diagnosis , Alphapapillomavirus/isolation & purification , Automation, Laboratory/standards , Cell Biology/organization & administration , Cervix Uteri/pathology , Cervix Uteri/virology , Colposcopy/methods , Female , Humans , Information Storage and Retrieval/legislation & jurisprudence , Information Storage and Retrieval/standards , Laboratories/organization & administration , Medical Audit , Medical Laboratory Personnel/education , Medical Laboratory Personnel/organization & administration , Medical Laboratory Personnel/standards , Specimen Handling/standards , United Kingdom , Uterine Cervical Neoplasms/virology , WorkforceABSTRACT
Immunoglobulin heavy chain (IgH) gene rearrangement analysis was performed on 27 fine needle aspiration (FNA) specimens (13 reactive hyperplasia, 11 B cell non-Hodgkin's lymphoma (B-NHL), one Hodgkin's disease and two suspicious of non-Hodgkin's lymphoma). Satisfactory amplification was achieved in 23/27 cases. A polyclonal pattern was seen in 14 cases (11 reactive hyperplasia, one B-NHL, one suspicious of lymphoma, one Hodgkin's disease). A monoclonal band was seen in nine cases (eight B-NHL, one reactive hyperplasia). Amplification was unsuccessful in four cases. Clonal analysis by PCR-based IgH gene rearrangement analysis can be successfully applied to FNA material and can be useful in diagnosis, but the results must be interpreted in conjunction with morphology and other ancillary information.
Subject(s)
Cloning, Molecular , Gene Rearrangement, B-Lymphocyte, Heavy Chain , Polymerase Chain Reaction , Biopsy, Needle/methods , Hodgkin Disease/genetics , Hodgkin Disease/pathology , Humans , Lymphoma, B-Cell/genetics , Lymphoma, B-Cell/pathology , Pseudolymphoma/genetics , Pseudolymphoma/pathologyABSTRACT
A case of cervical adenocarcinoma arising in diffuse mesonephric hyperplasia is presented. The hyperplastic and neoplastic tubules showed focal endocrine cell differentiation. Endocrine cells stained with the Grimelius technique, and were immunoreactive for chromogranin and serotonin. Antisera to additional specific hormones were negative. The natural history of this rare tumor is uncertain; the patient presented in this report is free of disease at 10-year follow-up.
Subject(s)
Mesonephroma/pathology , Uterine Cervical Neoplasms/pathology , Adult , Cell Differentiation , Cervix Uteri/pathology , Chromogranins/analysis , Female , Humans , Hysterectomy , Immunoenzyme Techniques , Keratins/analysis , Mesonephroma/surgery , Serotonin/analysis , Staining and Labeling , Uterine Cervical Neoplasms/surgery , Uterus/pathology , Vimentin/analysisABSTRACT
The cervical cytology and histology specimens from 200 patients referred to colposcopy with borderline nuclear abnormality were reviewed. Human papillomavirus (HPV)-associated changes were identified in 103 of 200 (53%) referral smears and in 139 of 150 (91%) biopsy specimens. Cervical intraepithelial neoplasia (CIN) was less frequently diagnosed on review compared with the original histopathology reports (30.7% vs 45.4%); the discrepancy was largely attributable to a lower incidence of CINI. There was agreement in the grading of borderline nuclear abnormality in 161 of 200 referral smears following review. Twenty-three smears were upgraded to mild dyskaryosis, whereas 16 were reclassified as negative.
Subject(s)
Cervix Uteri/pathology , Vaginal Smears , Female , Humans , Papillomaviridae , Papillomavirus Infections/diagnosis , Papillomavirus Infections/pathology , Tumor Virus Infections/diagnosis , Tumor Virus Infections/pathology , Uterine Cervical Diseases/diagnosis , Uterine Cervical Diseases/pathology , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/pathology , Uterine Cervical Dysplasia/diagnosis , Uterine Cervical Dysplasia/pathologyABSTRACT
The cytological and histological findings of a case of breast carcinoma with osteoclast-like giant cells are presented. A fine needle aspiration specimen demonstrated the characteristic combination of malignant epithelium and reactive multinucleated cells and enabled identification of this rare variant of breast cancer. Immunocytochemical studies using the monoclonal antibody KP1[CD68] support a histiocytic origin for the osteoclast-like cells.
Subject(s)
Breast Neoplasms/pathology , Giant Cells , Osteoclasts/pathology , Aged , Aged, 80 and over , Biopsy, Needle , Female , Humans , ImmunohistochemistryABSTRACT
It has been suggested that the coagulation defect in haemophilia A protects these patients from coronary artery disease. We report two patients with severe haemophilia, who each had severe atheroma localised to the coronary arteries. Both patients had received only small amounts of factor VIII concentrates in their lifetime because of various social reasons. In one case death resulted from myocardial infarction secondary to an occlusive thrombus in a coronary artery. The implications of these findings to the pathogenesis of vascular disease are discussed.
