ABSTRACT
Three groups of 15 goats each were immunised against contagious caprine pleuropneumonia (CCPP) using sonicated antigens of the F-38 strain of mycoplasma incorporated in incomplete Freund's adjuvant (IFA), emulsified in aluminium hydroxide and phosphate buffered saline respectively. Three months after immunisation, five goats from each group were challenged by the in-contact method. The goats immunised with the antigen incorporated in IFA were all solidly immune to the challenge whereas only two of five of the goats in the other two groups were protected. When the remaining 10 animals from each group were challenged six months after immunisation, those immunised with the antigen in IFA were still solidly immune while only two goats from each of the other two groups were protected. These results show that effective immunity against CCPP caused by the F-38 strain can be induced by vaccination with sonicated F-38 antigens emulsified in IFA.
Subject(s)
Antigens, Bacterial/immunology , Goats/immunology , Mycoplasma Infections/veterinary , Mycoplasma/immunology , Pleuropneumonia, Contagious/prevention & control , Animals , Antibodies, Bacterial/biosynthesis , Hemagglutination Tests/veterinary , Immunity , Immunization/veterinary , Pleuropneumonia, Contagious/immunology , SonicationABSTRACT
Passive haemagglutination and complement fixation tests were used to assess the antibody response of goats to the F-38 strain of mycoplasma. The passive haemagglutination test was found to have a positive correlation (r:0.89) with the complement fixation test in detection of antibody to strain F-38. Whereas passive haemagglutination was found more sensitive than the complement fixation test, it was less specific.
Subject(s)
Complement Fixation Tests/veterinary , Goats , Hemagglutination Tests/veterinary , Mycoplasma Infections/veterinary , Pleuropneumonia, Contagious/diagnosis , Animals , Mycoplasma/immunology , Pleuropneumonia, Contagious/etiology , Species SpecificityABSTRACT
A combination of dihydrostreptomycin sulphate (250 mg/ml) and penicillin G procaine (200,000 iu/ml) was used to treat contagious caprine pleuropneumonia caused by F38 strain of mycoplasma. A single dose of either 20, 30, 40 or 50 mg/kg body weight of the dihydrostreptomycin sulphate led to the recovery of the treated goats. The recovered goats did not transmit CCPP to susceptible goats housed with them for 2 months. The goats which recovered were found to be solidly immune to an in-contact challenge in which all the control goats died of CCPP. The treated and recovered goats were found not to be carriers of the organism.
