Effect of Ag doped MnO2 nanostructures suitable for wastewater treatment and other environmental pollutant applications.

A modest sol-gel method has been employed to prepare the pure and Ag doped MnO2 nanoparticles and methodologically studied their physical, morphological, and photosensitive properties through XRD, TEM, EDAX, Raman, UV, PL and N2 adsorption - desorption study. Tetragonal crystalline arrangement with spherical nanoparticles was found out through XRD and TEM studies. The EDAX studies further supported that formation Ag in the MnO2 crystal matrix. The bandgap energy of Ag doped MnO2 was absorbed through UV spectra. Photo -generated recombination process and surface related defects were further recognized by PL spectra. Through visible light irradiation, the photo - degradation of methyl orange (MO) and phenol dye solutions were observed. The optimum condition of (10 wt% of Ag) Ag doped MnO2 catalyst showed tremendous photocatalytic efficiency towards MO than phenol under same experimental study.

A novel biogenic Allium cepa leaf mediated silver nanoparticles for antimicrobial, antioxidant, and anticancer effects on MCF-7 cell line.

In the present study, Allium cepa leaf extract was utilized to reduce the silver nitrate into the nanoscale range of silver ions (Ag NPs). The biosynthesized Ag NPs were extensively characterized by X-ray diffraction analysis (XRD), Dynamic light scattering analysis (DLS), UV-Visible spectroscopy (UV-vis), Transmission electron microscopy (TEM), Energy dispersive X-ray analysis (EDX) and Fourier transform infrared spectroscopy (FTIR). The antioxidant activity of synthesized Ag NPs was verified by DPPH assay. From the results obtained from XRD and DLS studies, the size of Ag NPs was determined to be around 54.3 nm. The measured zeta potential value of -19.1 mV confirms the excellent stability of biosynthesized Ag NPs. TEM analyses reveal that the biosynthesized Ag NPs have a spherical structure of 13 nm in size. The presence of various functional groups was confirmed through FTIR studies and EDAX verifies the weight percentage of silver content in biosynthesized nanoparticles to be 30.33%. In the present study, anti-cancer activity was carried out by using breast cancer cell line MCF-7. Further, silver nanoparticles exhibited antimicrobial effectiveness against gram-positive Bacillus cereus and gram-negative Escherichia coli. The MTT assay also showed better cytotoxic activity against the MCF- 7 cell line.

Hollow selenium nanoparticles from potato extract and investigation of its biological properties and developmental toxicity in zebrafish embryos.

A facile synthesis of hollow selenium nanoparticles (hSeNPs) was prepared using potato starch as a reducing and capping agent. The morphological and structural characters of the hSeNPs were characterised by ultraviolet-visible spectroscopy (UV), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDX) and zeta potential analyser. The optical characteristics of hSeNPs were confirmed by UV. The presence of various functional groups in the hSeNPs suspension was confirmed by FTIR. The SEM results suggested that the synthesised hSeNPs were uniformly distributed and circular in shape with a hollow. The average size of the hSeNPs was found to be around 115 nm. The EDX analysis also confirmed the presence of hSeNPs in the sample. The zebrafish embryos were treated with hSeNPs of various concentrations ranging from 10 to 50 µg/ml. Abnormalities such as improper heartbeat, embryo sac oedema, ocular oedema and head oedema were noted at higher concentrations (30-50 µg/ml). A concentration-dependent antioxidant activity of hSeNPs was observed. The hSeNPs showed good antibacterial activity against gram-positive Bacillus subtilis and gram-negative Escherichia coli. The results of this study indicate that potato extract reduces the toxicity of hSeNPs and lower concentrations of hSeNPs could be used for various biomedical applications in near future.

A novel biocompatible chitosan-Selenium nanoparticles (SeNPs) film with electrical conductivity for cardiac tissue engineering application.

Cardiomyopathy is the leading cause of mortality in the world and economic burdens on national economies. A cardiac patch approach aims at regenerating an infracted heart by providing healthy functional cells to the injured region via a film carrier substrate, and providing mechanical and electrical support. Selenium acts as an important element in the prevention and treatment of cardiovascular diseases but their health-related effects have not been fully explored. Limitation is the fact that cardiac electrophysiology was only globally personalized, thus missing the potential localized pathological features in vivo. The epidemiological aspects of plasma levels of selenium and other lipid parameters in cardiomyopathy patients (30 nos) from South Tamilnadu, India were studied. The epidemiological data showed significant differences between plasma selenium, Glutathione per oxidase (Gpx) and High reactive-C Protein in cardiomyopathy patients when compared to the control. As a novel approach, in the present study chitosan-Selenium nanoparticles (SeNPs) film was used to produce electrical conductivity in the cardiac patches. The prepared chitosan-SeNPs film was characterized by Scanning Electron microscopy with Energy Dispersive X ray spectrum (SEM-EDX). The electrical and mechanical properties of the chitosan-SeNPs film were also studied. The chitosan-SeNPs film had compression of elastic modulus (67.1% elongation) and tensile strength of 419 kPa. The electrical conductivity of chitosan-SeNPs film was measured as 0.0055S cm-1. The H9C2 cells were very well grown in chitosan-SeNPs film and proliferated. In our study, we confirm the potential of SeNPs-chitosan film for use as substrates to grown cellular behavior via electrical stimulation, mechanical strength and as biocompatible film for cardiac tissue engineering applications.

A novel one-pot green synthesis of selenium nanoparticles and evaluation of its toxicity in zebrafish embryos.

Over the last 50 years, compelling evidence has accumulated on the beneficial role of selenium in human health. In the present study, different proteins were evaluated as reducing agents for the eco-friendly synthesis of selenium nanoparticles from an aqueous solution of sodium selenite. This method is a simple, low cost green synthesis alternative to chemical synthesis. The high conversion of selenium ions to selenium nanoparticles (SeNPs) was achieved by a reaction mixture of 0.1 g bovine serum albumin and 0.1 g sodium selenite at a reaction temperature of 121°C for 20 min duration. The selenium nanoparticles were characterized by fourier transform infrared (FTIR), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy. The FTIR spectral bands were sharp with strong absorption peaks at 1649 and 1551 cm(-1). SEM analysis of the synthesized selenium nanoparticles clearly showed the spherical shape with an average size ranging from 500 to 600 nm. The toxicity of SeNPs was evaluated using zebrafish embryos as a model system. SeNPs induced malformations in zebrafish embryos in a concentration-dependent manner. Selenium nanoparticles at 15-25 µg/ml concentration caused pericardial edema, tail malformation and decrease in heart rate in zebrafish embryos. Treatments with lower concentrations did not alter the heart rate or display any heart abnormalities. This study underlines the importance of identifying optimal SeNP concentration that could have potential therapeutic applications.

Comparative analysis of cardiovascular effects of selenium nanoparticles and sodium selenite in zebrafish embryos.

Selenium acts as an important element in the prevention and treatment of cardiovascular diseases but their health-related effects have not been fully explored. As a novel attempt, zebrafish embryos were treated separately with SeNPs (5-25 µg/ml) and sodium selenite (5-25 µg/ml) starting at early blastula stage. Abnormalities were also observed in the morphology of the zebrafish embryos. The SeNPs-treated embryos exhibited concentration-dependent increased in mortality, pericardial edema, and cardiac arrhythmia. In contrast, sodium selenite showed no significant malformation effect in developing zebrafish embryos. The results of the present study conclude that the SeNPs were more toxic than sodium selenite. The results also suggest that lower concentrations of SeNPs and sodium selenite can be used as possible therapeutic agents for cardiovascular-related problems.

Sodium selenite/selenium nanoparticles (SeNPs) protect cardiomyoblasts and zebrafish embryos against ethanol induced oxidative stress.

Alcoholic cardiomyopathy is the damage caused to the heart muscles due to high level of alcohol consumption resulting in enlargement and inflammation of the heart. Selenium is an important trace element that is beneficial to human health. Selenium protects the cells by preventing the formation of free radicals in the body. In the present study, protein mediated synthesis of SeNPs was investigated. Two different sizes of SeNPs were synthesized using BSA and keratin. The synthesized SeNPs were characterized by scanning electron microscopy (SEM) with elemental composition analysis Energy Dispersive X-ray spectroscopy(EDX) and X-ray diffraction (XRD). This study demonstrates the in vitro and in vivo antioxidative effects of sodium selenite and SeNPs. Further selenium and SeNPs were evaluated for their ability to protect against 1% ethanol induced oxidative stress in H9C2 cell line. The selenium and SeNPs were found to reduce the 1% ethanol-induced oxidative damage through scavenging intracellular reactive oxygen species. The selenium and SeNPs could also prevent pericardial edema induced ethanol treatment and reduced apoptosis and cell death in zebrafish embryos. The results indicate that selenium and SeNPs could potentially be used as an additive in alcoholic beverage industry to control the cardiomyopathy.